• Title/Summary/Keyword: B.C.G

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Survival, isolation and characterization of Bacillus cereus from Sunshik (선식에서 Bacillus cereus의 분리 및 특성 조사와 열에 대한 사멸률 연구)

  • Cho, Yong-Sun;Jung, Eun-Young;Lee, Myung-Ki;Yang, Cheul-Young;Shin, Dong-Bin
    • Journal of Food Hygiene and Safety
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    • v.23 no.4
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    • pp.343-347
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    • 2008
  • Recently, Sunshik has been issued because of easy-cook and well-being food. Sunshik basically was made of the heated cereals. Amount of spore-forming Bacillus cereus was detected and it has been caused some problem of food safety. B. cereus was isolate from 57 out of 161 Sunshik samples resulting in the isolation rate of 35.4%. Quantitative analysis of 57 samples showed that 21 samples were less than 100 CFU/g, 33 samples were between 100 and 1,000 CFU/g and distinctively even 3 (1.9%) samples had over 1,000 CFU/g. Typical morphology of B. cereus isolated from Sunshik was observed on MYP agar and then further characteristics was identified by using VITEK 2 (Biomeriux, France). 53 strains out of 57 strains isolated from Sunshik (about 93.0%) produced diarrheal enterotoxin in brain heart infusion broth which was detected by the Bacillus cereus enterotoxin reversed passive latex agglutination test kit (Oxoid England). The D-values of the B. cereus spores were $75^{\circ}C$ (37.1mim), $80^{\circ}C$ (22.5mim), $85^{\circ}C$ (4.9mim), and $90^{\circ}C$ (3.1mim) respectively. The Z-value was calculated $12.8^{\circ}C$ in Sunshik sample inoculated with B. cereus. Therefore, the management of B. cereus in Sunshik is required for the food-safety.

The Current Distribution of Revolution-Body Using Conjugate Gradient Method (C. G. M을 이용한 회전형 완전도체의 전류분포해석)

  • 이광천;김정기
    • Proceedings of the Korean Institute of Communication Sciences Conference
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    • 1987.04a
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    • pp.184-187
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    • 1987
  • The method of Conjugate Gradient(C.G.M) is applied to the solution of current distribution from body of revolution (B.O.R).Using the C.G.M it is possible to analyze electrically large arbtrarily oriented B.O.R. The fundamental differences between C.G.M and moment method are outlined. This method converges for any initial guess and this techniqe gurantees a monotonic convergence. Numerical results are presented for electromagnetic case which show good agreement with moment solution.

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Determination of Li generated from 10B(n·α)7Li reaction in Boric acid solution (붕산수용액에서 10B(n·α)7Li 핵반응에 의해 생성된 Li 정량)

  • Choi, Ke-Chon;Jung, Yongju;Yoon, Jei-Won;Kim, Won-Ho
    • Analytical Science and Technology
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    • v.16 no.6
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    • pp.443-449
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    • 2003
  • Thermal neutron irradiation experiment of boric acid solution was carried out using HANARO in following three conditions: (A) $^{10}B$ concentration = $203.0{\mu}g/mL$, irradiation time = 1 hr; (B) $^{10}B$ concentration = $381.4{\mu}g/mL$, irradiation time = 1 hr; (C) $^{10}B$ concentration = $381.4{\mu}g/mL$, irradiation time = 0.5 hr. The amount of lithium produced from $^{10}B(n{\cdot}{\alpha})^7Li$ reaction which was generated on neutron irradiation, was measured by flameless atomic absorption spectroscopy. The concentration of $^7Li$ measured in the three experiments was $0.18{\mu}g/mL$ (78.3% of theoretical value, $0.23{\mu}g/mL$) in (A), $0.31{\mu}g/mL$ (70.5% of theoretical value, $0.44{\mu}g/mL$) in (B) and $0.16{\mu}g/mL$ (71.6% of theoretical value, $0.22{\mu}g/mL$) in (C). The pH value of irradiated boric acid was shifted to considerably low. It is estimated that boric acid would be transformed into the polyborate fonn, by radiolysis products of water, which has high dissociation constant.

In vivo Radioprotective Effects of Basic Fibroblast Growth Factor in C3H Mice (Basic Fibroblast Growth Factor (bFGF)의 방사선보호작용에 대한 실험적 연구)

  • Kim, Yeon-Shil;Yoon, Sei-Chul
    • Radiation Oncology Journal
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    • v.20 no.3
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    • pp.253-263
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    • 2002
  • Purpose : In order to understand in vivo radiation damage modifying of bFGF on jejunal mucosa, bone marrow and the effect of bFGF on the growth of transplanted mouse sarcoma 180 tumor in mice. Materials and Methods : Mice were treated with $6\;{\mu}g$ of bFGF at 24 hours and 4 hours before exposing to 600 cGy, 800 cGy and 1,000 cGy total body irradiation (TBI), and then exposed to 3,000 cGy local radiation therapy on the tumor bearing thigh. Survival and tumor growth curve were plotted in radiation alone group and combined group of bFGF and irradiation (RT). Histologic examination was performed in another experimental group. Experimental groups consisted of normal control, tumor control, RT (radiation therapy) alone, $6\;{\mu}g$ bFGF alone, combined group of $3\;{\mu}g$ bFGF and irradiation (RT), combined group of $6\;{\mu}g$ bFGF and irradiation (RT). Histologic examination was peformed with H-E staining in marrow, jejunal mucosa, lung and sarcoma 180 bearing tumor. Radiation induced apoptosis was determined in each group with the DNA terminal transferase nick-end labeling method ($ApopTag^{\circledR}$ S7100-kit, Intergen Co.) Results : The results were as follows 1) $6\;{\mu}g$ bFGF given before TBI significantly improved the survival of lethally irradiated mice. bFGF would protect against lethal bone marrow syndrome. 2) $6\;{\mu}g$ bFGF treated group showed a significant higher crypt depth and microvilli length than RT alone group (p<0.05). 3) The bone marrow of bFGF treated group showed less hypocellularity than radiation alone group on day 7 and 14 after TBI (p<0.05), and this protective effect was more evident in $6\;{\mu}g$ bFGF treated group than that of $3\;{\mu}g$ bFGF treated group. 4) bFGF protected against early radiation induced apoptosis in intestinal crypt cell but might have had no antiapoptotic effect in bone marrow stem cell and pulmonary endothelial cells. 5) There was no significant differences in tumor growth rate between tumor control and bFGF alone groups (p>0.05). 6) There were no significant differences in histopathologic findings of lung and mouse sarcoma 180 tumor between radiation alone group and bFGF treated group. Conclusions : Our results suggest that bFGF protects small bowel and bone marrow from acute radiation damage without promoting the inoculated tumor growth in C3H mice. Improved recovery of early responding normal tissue and reduced number of radiation induced apoptosis may be possible mechanism of radioprotective effect of bFGF.

A NOTE ON DERIVATIONS OF A SULLIVAN MODEL

  • Kwashira, Rugare
    • Communications of the Korean Mathematical Society
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    • v.34 no.1
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    • pp.279-286
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    • 2019
  • Complex Grassmann manifolds $G_{n,k}$ are a generalization of complex projective spaces and have many important features some of which are captured by the $Pl{\ddot{u}}cker$ embedding $f:G_{n,k}{\rightarrow}{\mathbb{C}}P^{N-1}$ where $N=\(^n_k\)$. The problem of existence of cross sections of fibrations can be studied using the Gottlieb group. In a more generalized context one can use the relative evaluation subgroup of a map to describe the cohomology of smooth fiber bundles with fiber the (complex) Grassmann manifold $G_{n,k}$. Our interest lies in making use of techniques of rational homotopy theory to address problems and questions involving applications of Gottlieb groups in general. In this paper, we construct the Sullivan minimal model of the (complex) Grassmann manifold $G_{n,k}$ for $2{\leq}k<n$, and we compute the rational evaluation subgroup of the embedding $f:G_{n,k}{\rightarrow}{\mathbb{C}}P^{N-1}$. We show that, for the Sullivan model ${\phi}:A{\rightarrow}B$, where A and B are the Sullivan minimal models of ${\mathbb{C}}P^{N-1}$ and $G_{n,k}$ respectively, the evaluation subgroup $G_n(A,B;{\phi})$ of ${\phi}$ is generated by a single element and the relative evaluation subgroup $G^{rel}_n(A,B;{\phi})$ is zero. The triviality of the relative evaluation subgroup has its application in studying fibrations with fibre the (complex) Grassmann manifold.

Aptness for the Preparation of Liquid Yogurt by Lactobacilli isolated from Korean Liquid Yogurts (국내 액상발효유에서 분리한 유산균의 제품제조 적성조사)

  • So, Myeong-Hwan
    • Korean Journal of Food Science and Technology
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    • v.17 no.3
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    • pp.197-202
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    • 1985
  • In order to evaluate the aptness for the preparation of liquid-yogurt, microbiological properties of 8 Lactobacilli isolated from 7 Korean liquid-yogurts were investigated. To accelerate acid-producing ability in skim milk, L. bulgaricus D, L. casei A and L. casei E need to supply with glucose, and L. Casei A and L. csaei E also need milk-protein hydrolyzate, but L. jugurti B, L. jugurti C, L. jugurti G, L. helveticus F and L. acidophilus B did not need those supplements. In cultivation in skim milk supplemented with glucose, L. jugurti B, L. jugurti C, L. jugurti G. L. helveticus F and L. bulgaricus D showed rapidity in acid development and weakness in cell viability, but L. acidophilus B, L. casei A and L. casei E showed slowness in acid development and highness in cell viability. Liquid-yogurts made by L. casei A or L. casei E had no sedimentation during storage but those made by L. jugurti B, L. jugurti C, L. jngurti G, L. helveticus F, L. bulgaricus D, or L. acidophilus B had much sedimentation. In sensory evaluation, Liquid-yogurts made by L. casei A or L. casei E had good response but those made by L. jugurti B, L. jugurti C, L. jugurti G, L. helveticus F, L. bulgaricus D or L. acidophilus B did not. Among 8 Lactobacilli examined, L. casei A and L. casei E were thought to have more suitable properties for the preparation of liquid-yogurt though they required a long period of cultivation.

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Study on Utilization of Heterosis in Layer Chicken (산란계종의 잡잡강세리용에 관한 연구)

  • 오봉국;여정수;이정구;이문연
    • Korean Journal of Poultry Science
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    • v.7 no.2
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    • pp.28-36
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    • 1980
  • This study was carried out to estimate combining abilitie or economic traits in layer chickens. The data used in this study were the record of 10 single crosses produced by half diallel cross of 5 lines of Single Comb White Leghorns, such as A, B, C, K and S lines. Total 720 progenies of the crosses were reared at the Poultry Breeding Farm, College of Agriculture, Seoul National University from Feb. 1979 to August, 1980. Combining abilities were estimated by Grilling's mathematical model for the traits; age the first egg, total egg number, egg weight and body weight. The results attained from the studies were summarized as follows; In estimate of combining ability, an age at first egg of BS cross was largely due to significiantly higher general combining ability (G. C. A.) effect of B and S strains than Cand K strains in G. C. A. effect, and to specific combining ability (S. C. A) effect of B and S-trains. AB and BS crosses showed the highest egg Production. AB cross performance was result from high G.C.A. effect of A ana B strains. BS cross performance was result from high G. C. A. effect of B and high S. C. A. effect of BS cross. Specific combining ability effect in egg. weight was not statiscally significiant, but S strain showed high G. C. A. effect. A and B strains in body weight showed significantly low C. C. A. effect. From the above results, BS cross in an age at first egg, AB and BS crosses in egg Production, S strain in egg weight and AB cross in body weight were superior to other strains or crosses.

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Effects of Crown Diameter on Plant Growth and Fruit Yield in Strawberry (Fragaria × ananassa Duch.) (딸기 묘의 크라운 크기가 정식 후 식물체 생육 및 과실 수량에 미치는 영향)

  • Kang, Ho-Jong;Song, Hyun-Jin;Park, Soo-Jeong;Kim, Zhoo-Hyeon;Lee, Sang-Woo
    • Journal of agriculture & life science
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    • v.45 no.4
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    • pp.81-86
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    • 2011
  • This study was conducted to evaluate the influence of crown diameter on plant growth after planting and fruit yield. All nursery plants were classified into three classes: A, > 9 mm; B, 8~9 mm; C, < 8 mm. The A-class nursery plant plot showed better root and plant growth after planting compared to the B-class and C-class nursery plants. The A-class nursery plant plot was 10 days faster in first flowering time then those of the B-class and the C-class. Also, the A-class nursery plant plot was 2~3 days faster in the mean flowering time. The accumulative yield of the A-class nursery plant plot was 481 g per plant, that of the B-class was 445 g, and the C 422 g, which the nursery plant plot of A-class yielded more 8.1% and 13.9% respectively than B-class plot and C-class plot per plant. In early yield of to February; A-class nursery plant plot showed 23.3% higher yield than B-class nursery plant plot, and 39.1% higher the C-class nursery plant.

Chemical Components of Red, White and Extruded Root Ginseng (홍삼 . 백삼 및 압출성형 건조수삼의 성분특성)

  • Ha, Dae-Chul;Ryu, Gi-Hyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.2
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    • pp.247-254
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    • 2005
  • The objective of this study is to compare the chemical properties of red ginseng, white ginseng, and extruded ginseng. Six kinds of samples were prepared and examined their chemical components. The comparison among crude ash, crude lipid, and total sugar resulted insignificant difference. White ginseng had lower content of reducing sugar than those of extruded ginseng and red ginseng. Total amino acid was found relatively low in treatment A (sliced whole root and dried at 7$0^{\circ}C$). Total amino acid of treatment C (extruded dry whole root ginseng slices, moisture content 30%, barrel temperature 11$0^{\circ}C$, and screw speed 200 rpm) was higher than that of treatment B (extruded dry whole root slices, moisture content 25%, barrel temperature 11$0^{\circ}C$, and screw speed 200 rpm). Crude saponin of treatments A, B, C, D (white ginseng with skin), E (skinless white ginseng), and F (red ginseng) were 4.02, 4.77, 4.12, 3.56, 3.25, and 4.02%, respectively. Ginsenoside was contained similarly as crude saponin. The amount of ginsenoside in the treatment of A, B, C, D, E, and F was recorded respectively at 6.031, 8.108, 6.876, 7.978, 5.591, and 9.834 mg/g. A specific component in red ginseng, $R_{g3}$ was detected in treatment F. Maltol was detected in treatment Band F. Acidic polysaccharide was increased 2∼3% by extrusion process. In conclusion, extruded ginseng had similar components to those of red ginseng.