• Title/Summary/Keyword: B.C.G

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In Vivo Analysis of fadB Homologous Enzymes Involved in Biosynthesis of Polyhydroxyalkanoates in Recombinant Escherichia coli (재조합 대장균에서 fadB 유사효소의 Polyhydroxyalkanoates 합성에 미치는 역할의 규명)

  • 최종일;박시재;이상엽
    • KSBB Journal
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    • v.19 no.4
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    • pp.331-334
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    • 2004
  • In vivo characterization of FadB homologous enzymes including PaaG, YdbU and YgfG for medium-chain-length (MCL) polyhydroxyalkanoate (PHA) biosynthesis was carried out in fadB mutant Escherichia coli. Previously, it was reported that amplification of FadB homologous enzymes such as PaaG and YdbU in fadB mutant E. coli resulted in enhanced biosynthesis of MCL-PHA by greater than two fold compared with control strain. In this study, we constructed paaG fadB double mutant E. coli WB114 and ydbU fadB double mutant E. coli WB115 to investigate the roles of PaaG and YdbU in biosynthesis of MCL-PHA. Inactivation of paaG and ydbU genes in fadB mutant E. coli harboring Pseudomonas sp. 61-3 phaC2 gene reduced the MCL-PHA production to 0.16 and 0.16 PHA g/L, respectively from 2 g/L of sodium decanoate, which are much lower than 0.43 PHA g/L obtained with fadB mutant E. coli WB101 harboring the phaC2 gene. Also, we identified new FadB homologous enzyme YgfG, and examined its roles by overexpression of ygfG and construction of ygfG fadB double mutant E. coli WB113.

A Study on the Occurrence of Benzo(a)pyrene in Fats and oils by Heat Treatment(I) (유지가열시 Benzo(a)pyrene 생성에 관한 연구(I) -대두유가열시-)

  • 김인숙;안명수;장대경
    • Korean journal of food and cookery science
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    • v.9 no.4
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    • pp.323-328
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    • 1993
  • Benzo(a)pyrene [B(a)P], one of the polycyclic aromatic hydrocarbons (PAHs) is known as a potent carcinogen. As lipid consumption increases recently, the toxic effect of overheated lipid foods and fats & oils were reported increasingly. In this study, the contents of B(a)p, other PAHs and rancidities of soybean oil were determined, and then the proper heating temperature, time and frequency were recommended, The work was carried out using soybean oil heated at $180\pm$5^{\circ}C$$, $200\pm$5^{\circ}C$$, and $300\pm$5^{\circ}C$$,for 50 hours. Acid Value(AV) and Conjugated Diene Value of samples were determined. The contents of B(a)P and other PAHs contents of all samples were masured by HPLC/UV method. The results obtained were as follows; Each content of PAHs in the fresh soybean oil was: Pyr 1.093, B(a)A 0.986, Ch 1.147, DMBA 1.082, B(e)P 0.664, Per 1.135, B(a)P 0.146, DBA 1.053, 3-MC 0.05 rg/kg. When the soybean oil was heated at $180\pm$5^{\circ}C$$, for 10, 20, 30, and 50 hours, B(a)P conterlts in heated soybean oils were 0.391, 0.692, 0.451, and 0.372 $\mu\textrm{g}$/kg respectively. Acid value of them were 0.26, 0.26, 0.29, and 0.33, and conjugated diene value was 0.67, 0.76, 0.99, and 1.04, respectively. When the soybean oil was heated at $200\pm$5^{\circ}C$$,and $300\pm$5^{\circ}C$$,for 10, 20, 30, and 50 hours, B(a)P coiltents in soybean oil heated at $200\pm$5^{\circ}C$$,were 0.844, 0.512, 0.479 and 0.247 Ig/kg respectively, Acid value 0.22, 0.21, 0.23 and 0.51 and CDNV 0.39, 0.49, 3.27, and 3.89. B(a)P contents in soybean oil heated at $300\pm$5^{\circ}C$$,were 0.466, 0.706, 0.607 and 0.247$\mu\textrm{g}$/kg respectively, Acid value 0.47, 1.57, 3.90, and 6.42 and CDNV 0.65, 2.15, 3.00, and 3.88.

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On the Basis Number of the Semi-Strong Product of Bipartite Graphs with Cycles

  • Jaradat, M.M.M.;Alzoubi, Maref Y.
    • Kyungpook Mathematical Journal
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    • v.45 no.1
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    • pp.45-53
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    • 2005
  • A basis of the cycle space C (G) is d-fold if each edge occurs in at most d cycles of C(G). The basis number, b(G), of a graph G is defined to be the least integer d such that G has a d-fold basis for its cycle space. MacLane proved that a graph G is planar if and only if $b(G)\;{\leq}\;2$. Schmeichel showed that for $n\;{\geq}\;5,\;b(K_{n}\;{\bullet}\;P_{2})\;{\leq}\;1\;+\;b(K_n)$. Ali proved that for n, $m\;{\geq}\;5,\;b(K_n\;{\bullet}\;K_m)\;{\leq}\;3\;+\;b(K_n)\;+\;b(K_m)$. In this paper, we give an upper bound for the basis number of the semi-strong product of a bipartite graph with a cycle.

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Applications of Convolution Operators to some Classes of Close-to-convex Functions

  • Noor, Khalida Inayat
    • Honam Mathematical Journal
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    • v.10 no.1
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    • pp.23-30
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    • 1988
  • Let C[C, D] and $S^{*}[C,\;D]$ denote the classes of functions g, g(0)=1-g'(0)0=0, analytic in the unit disc E such that $\frac{(zg{\prime}(z)){\prime}}{g{\prime}(z)}$ and $\frac{zg{\prime}(z)}{g(z)}$ are subordinate to $\frac{1+Cz}{1+Dz{\prime}}$ $z{\in}E$, respectively. In this paper, the classes K[A,B;C,D] and $C^{*}[A,B;C,D]$, $-1{\leq}B<A{\leq}1$; $-1{\leq}D<C{\leq}1$, are defined. The functions in these classes are close-to-convex. Using the properties of convolution operators, we deal with some problems for our classes.

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INDEX AND STABLE RANK OF C*-ALGEBRAS

  • Kim, Sang Og
    • Korean Journal of Mathematics
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    • v.7 no.1
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    • pp.71-77
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    • 1999
  • We show that if the stable rank of $B^{\alpha}$ is one, then the stable rank of B is less than or equal to the order of G for any action of a finite group G. Also we give a short proof to the known fact that if the action of a finite group on a $C^*$-algebra B is saturated then the canonical conditional expectation from B to $B^{\alpha}$ is of index-finite type and the crossed product $C^*$-algebra is isomorphic to the algebra of compact operators on the Hilbert $B^{\alpha}$-module B.

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Soil Microarthropods at the Kwang Yang Experiment Plantation -1. Composition of Oribatid Mites (Acari: Cryptostigmata)- (서울대 광양연습림내 토양 미소 절지동물에 관한 연구 -1. 날개응애의 종조성)

  • Kwak, Joon Soo
    • The Korean Journal of Ecology
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    • v.10 no.1
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    • pp.23-31
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    • 1987
  • This study was carried out to investigate by the MGP analysis the composition of oribatid mite in coniferous and broad-leaved forests, Kwang Yng experiment plantation, Chola Nam Do Province, southern part of Korea. In these study area, 157 species of oribatid mites (Acari: Cryptogimata) were identified. Among them 6 species such as; Brachychochthonius jugatus JACOT, B hungaricus BALOGH, Eremulus translamealtus BALOGH et. MAHUNKA, Brachioppiella ctenifera GOLOSOVA, Striatoppia opuntiseta BALOGH et. MAHUNKA, Suctobelba perdentata baculifer BALOGH et. MAHUNKA have not been described in Korea. Species compositions were as follows; Among them 69 species were found in all the six sampling sites, 33 species scattered sporadically in all the area, 12 species found in coniferous forest, 10 species in broad-leaved forest only, and 33 species sampled at one site. According to the MGP analysis I, site B-1, B-2, and B-3 were found to be "Type G" and site C-1, C-2, and C-3 were "Type MG". According to the MGP analysis II, site B were found to be all "Type G". But site C-1 was "Type MG", C-2 was "Type M" and C-3 was "Type G" suggesting that "Group M" increased in the site C.e G" suggesting that "Group M" increased in the site C. in the site C.

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A Study on the Prediction Methods of Domestic e-Commerce Market Size (국내전자상거래 시장규모 예측방법에 관한 연구)

  • Choi, Kyo-Won
    • The Journal of Society for e-Business Studies
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    • v.9 no.4
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    • pp.1-17
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    • 2004
  • We guarantee the significance of the provided prediction model and predicted figures from the experts consulting group and we product the prediction figures of the domestic e-commerce market size in future by business subjects, BtoB, BtoG and BtoC. Besides, we do predict by the high raked 6 merchandises in the case of BtoC market size prediction. We use the KNSO(Korea National Statistical Office) BtoB, BtoG and BtoC data to ensure the significance of data.

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The Structure of Econazole Nitrate (에코나졸 나이트레이트의 구조)

  • Seo, Il-Hwan;Jo, Seong-Il;Park, Gwon-Il
    • Korean Journal of Crystallography
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    • v.1 no.1
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    • pp.14-18
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    • 1990
  • Econazole nitrate, 1-{2-[(4-chlorophenyl)methoxy]-2-(2,4-dichlorophenyl) ethy1}-1H-imidazole mono-nitrate.C18 H16 CI13 N3 O4 Mw=444.7 Monoclinic P/2₁c,a=17.337(4)A, b=15.191(5), c=7.601(3)A, β=91.72(2)', V=2000.9A3, Z=4, Dc=1.49g/cm3, Dm=1.45g/cm3(mo-ka)= 0.7107A, μ=4.31cm-1, F(000)=912.0, T=298'K, final R=0.061 for 1330 unique observed reflection. Each of the three ring system for the stars with B,A and C ring in order whilst A and C ring of econazole lie close to the same plane which is nearly 60˚with B ring. The hydrogen binding nitrogen of C ring and oxygen of nitrate contributes to stailization of econazole nitrate. Intr and intermolecular distances and angles are within the values recorded for simiar compounds.

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Studies on the Ginseng Plants(III) -Radioactive Sodium $Acetate-U-C^{14}$ Feeding Experiments- (인삼식물(人參植物)에 관한 연구(III) -동위원소화합물(洞位元素化合物) Sodium $Acetate-U-C^{14}$을 투여한 실험-)

  • Kim, Jung-Yun;Staba, E. John
    • Korean Journal of Pharmacognosy
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    • v.5 no.2
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    • pp.111-124
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    • 1974
  • The radioactive compound sodium $acetate-U-C^{14}\;(C^{14}-acetate)$ was administered to two- and four-year-old July and September American ginseng (Araliaceae, Panax quinquefolium L.) plants and cuttings. The $C^{14}-acetate$ uptake was approximately 99%. The autoradiochromatograms suggest that the saponins isolated by preparative thin-layer chromatography contained impurities, especially those isolated from the leaf and stem extracts. The root and fruit methanol extracts yielded relatively pure saponins. The large amounts of panaquilin B and its proximity to panaquilin C on preparative thin-layer plates resulted in some admixing. The average concentration (% plant dry weight) of semi-purified saponins were high in the leaves (13.8%), as compared to fruits (9.8%), stems (7.9%) and roots (6.3%). The average percentage of $C^{14}-acetate$ incorporation into panaquilins was 4.8%. The average percentage of $C^{14}-acetate$ incorporation into panaquilins B and C was higher (1.40% and 1.13%, respectively) than that into panaquilins C, (d), G-1 and G-2 (0.75%, 0.65%, 0.13% and 0.53%, respectively). Panaquilin synthesis may be depending upon the part, collection period and age of the plant. The average percentage of $C^{14}-acetate$ incorporation into panaquilin B is high in roots (0.58%) and stems (0.48%); that into panaquilins C and (d) high in leaves (0.40% and 0.45%, respectively); and that into panaquilin E high in roots and leaves (0.55% and 0.50%, respectively). Panaquilin G-2 was synthesized in all parts of plants. The panaquilins appear to be biosynthesized more actively in July than September (exception-panaquilin G-1). Panaquilins B, C and G-1 may be biosynthesized more actively in four-year-old plants and panaquilins (d) and E more actively in two-year-old plants. The results from expectance with cuttings suggest that the panaquilins are synthesized de novo in the above-ground parts of ginseng plants, and that panaquilin G-1 may be synthesized de novo in the leaf. It is known from the tissue culture studies that panaquilins are produced by leaf, stem and root callus tissues and cailus-root cultures of American and Korean ginseng plants. Panaquilins may actively be synthesized de novo in most any cell or organ of the ginseng plants. It was verified that $C^{14}-acetate$ was incorporated into the panaxadiol portions of the panaquilins of two-year-old plants (sp. act. 0.56 mmcCi/mg) and four-year-old plants $(sp.\;act.\;0.54\;m{\mu}Ci/mg)$.

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Real-time Implementation of Variable Transmission Bit Rate Vocoder Improved Speech Quality in SOLA-B Algorithm & G.729A Vocoder Using on the TMS320C5416 (TMS320C5416을 이용한 SOLA-B 알고리즘과 G.729A 보코더의 음질 향상된 가변 전송률 보코더의 실시간 구현)

  • Ham, Myung-Kyu;Bae, Myung-Jin
    • Speech Sciences
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    • v.10 no.3
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    • pp.241-250
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    • 2003
  • In this paper, we implemented the vocoder of variable rate by applying the SOLA-B algorithm to the G.729A to the TMS320C5416 in real-time. This method using the SOLA-B algorithm is that it is reduced the duration of the speech in encoding and is played at the speed of normal by extending the duration of the speech in decoding. But the method applied to the existed G.729A and SOLA-B algorithm is caused the loss of speech quality in G.729A which is not reflected about length variation of speech. Therefore the proposed method is encoded according as it is modified the structure of LSP quantization table about the length of speech is reduced by using the SOLA-B algorithm. The vocoder of variable rate by applying the G.729A and SOLA-B algorithm is represented the maximum complexity of 10.2MIPS about encoder and 2.8MIPS about decoder in 8kbps transmission rate. Also it is evaluated 17.3MIPS about encoder, 9.9MIPS about decoder in 6kbps and 18.5MIPS about encoder, 11.1MIPS about decoder in 4kbps according to the transmission rate. The used memory is about program ROM 9.7kwords, table ROM 4.69kwords, RAM 5.2kwords. The waveform of output is showed by the result of C simulator and Bit Exact. Also, the result of MOS test for evaluation of speech quality of the vocoder of variable rate which is implemented in real-time, it is estimated about 3.68 in 4kbps.

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