• Journal of Microbiology and Biotechnology
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• v.7 no.4
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• pp.229-236
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• 1997

A gene, $phbC_{2.4.1}$ encoding poly-3-hydroxybutyric acid (PHB) synthase of Rhodobacter sphaeroides 2.4.1 was cloned by employing heterologous expression in Escherichia coli. R. sphaeroides chromosomal DNA partially digested with MboI was cloned in pUC19 followed by mobilization into E. coli harbouring $phbA,B_{AC}$ in pRK415, which code for ${\beta}$-ketothiolase and acetoacetyl CoA reductase of Alcaligenes eutrophus, respectively. Two E. coli clones carrying R. sphaeroides chromosomal fragment of $phbC_{2.4.1}$ in pUC19 were selected from ca. 10,000 colonies. The PHB-producing colonies had an opaque white appearance due to the intracellular accumulation of PHB. The structure of PHB produced by the recombinant E. coli as well as from R. sphaeroides 2.4.1 was confirmed by [$H^{+}$]-nuclear magnetic resonance (NMR) spectroscopy. Restriction analysis of the two pUC19 clones revealed that one insert DNA fragment is contained as a part of the other cloned fragment. An open reading frame of 601 amino acids of $phbC_{2.4.1}$ with approximate M.W. of 66 kDa was found from nucleotide sequence determination of the 2.8-kb SaiI-PstI restriction endonuclease fragment which had been narrowed down to support PHB synthesis through heterologous expression in the E. coli harbouring $phbA,B_{AC}$. The promoter (s) of the $phbC_{2.4.1}$ were localized within a 340-bp DNA region upstream of the $phbC_{2.4.1}$ start codon according to heterologous expression analysis.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.3C
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• pp.234-240
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• 2007

In this paper, low complexity block constrained trellis coded quantization (BC-TCQ) structures are introduced, and a predictive BC TCQ encoding method is developed for quantization of line spectrum frequencies (LSF) parameters for narrowband speech coding applications. Trellis-coded quantization(TCQ) is a form of VQ that builds the VQ codebook from interleaved constituent scalar quantization codebooks. The performance is compared to the other VQ, demonstrating reduction in spectral distortion and significant reduction in encoding complexity. The predictive BC-TCQ is about 0.47107 dB superior to the IS-641 split-VQ, 26bits/frame, in spectral distortion sense. The BC-TCQ is 64.54%, 76.93%, 2.35% of the IS-641 split-VQ, respectively, in the complexity of the additions, multiplies, comparisons.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.5C
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• pp.503-511
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• 2006

H.264 adopts new coding tools such as intra-prediction, loop filter, etc. The adoption of these tools enables an H.264-coded bitstream to have more information compared with previous standards. In this paper we proposed an effective spatial error concealment method for H.264. Among the information included in an H.264-coded bitstream, we use intra-mode for recovering a damaged block. This is because prediction direction in intra-mode is highly correlated to the edge direction of a lost macroblock. We first estimate the edge direction using intra-modes of blocks adjacent to a lost macroblock, and classify the area in a damaged macroblock into the edge and the flat area. And then our method recovers pixel values in the edge area using edge-directed interpolation, and recovers pixel values in the flat area using weighted interpolation. Simulation results show the proposed method yields better video quality than conventional approaches by 0.35 to 5.48 dB.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.42 no.5 s.305
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• pp.115-122
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• 2005

There are some problems in directly applying the conventional MPEG bit-stream based watermarking schemes to the bit-stream of a new compression standard, H.264/AVC. In this paper we analyze the effects of the conventional DCT-based watermarking scheme to H.264/AVC, especially in terms of image distortions and bit-rate changes. It turns out that the intra-frame prediction md CAVLC of H.264/AVC with the watermarking worsen the image distortions and bit-rate changes. The experiment results show on average 28.17dB decrease in PSNR and 56.71% increase in bit-rate over all QPs.

• BMB Reports
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• v.40 no.1
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• pp.22-28
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• 2007

MyoD, expressed in skeletal muscle lineages of vertebrate embryo, is one of muscle-specific basic helix-loop-helix (bHLH) transcription factors, which plays a key role in the determination and differentiation of all skeletal muscle lineages. In this study, a cDNA of grass carp MyoD was cloned and characterized from total RNA of grass carp embryos by RT-PCR. The full-length cDNA of grass carp MyoD is 1597 bp. The cDNA sequence analysis reveals an open reading frame of 825 bp coding for a protein of 275 amino acids, which includes a bHLH domain composed of basic domain (1-84th amino acids) and HLH domain (98-142th amino acids), without signal peptide. Then the MyoD cDNA of grass carp was cloned to yeast expression vector pPICZ$\alpha$A and transformed into P. pastoris GS115 strain, the recombinant MyoD protein with a molecular weight of about 31KD was obtained after inducing for 2d with 0.5% methanol in pH 8.0 BMGY medium, and the maximum yield was about 250 mg/L in shaking-flask fermentation. The results were expected to benefit for further studies on the crystal structure and physiological function of fish MyoD.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.12C
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• pp.1055-1061
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• 2008

H.264 is the prominent video coding standard in various applications such as real-time streaming and digital multimedia broadcasting, since it provides enhanced compression performance, error resilience tools, and network adaptation. Compression efficiency of H.264 has been improved, however, it requires more computing and memory access than traditional methods. In this paper we proposed adaptive intra fast algorithm for real-time video surveillance system reducing the encoding complexity of H264/A VC. For this aim, temporal interrelationship between macroblock in the previous and the current frame is used to decide the encoding mode of macroblock fast. As a result, though video quality was deteriorated a little, less than 0.04dB, and bit rate was somewhat increased in suggested method, however, proposed method improved encoding time significantly and, in particular, encoding time of an image with little changes of neighboring background such as surveillance video was more shortened than traditional methods.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.26 no.12A
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• pp.2027-2035
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• 2001

In this paper, we present an audio stream delivery using the AMR (Adaptive Multi-Rate) coder that was adopted by ETSI and 3GPP as a standard vocoder for next generation IMT-2000 service in which includes combined sender (FEC) and receiver reconstruction technique in the Internet. By use of the media-specific FEC scheme, the possibility to recover lost packets can be much increased due to the addition of repair data to a main data stream, by which the contents of lost packets can be recovered. The AMR codec is based on the code-excited linear predictive (CELP) coding model. So we use a frame erasure concealment for CELP-based coders. The proposed scheme is evaluated with ITU-T G.729 (CS-ACELP) coder and AMR - 12.2 kbit/s through the SNR (Signal to Noise Ratio) and the MOS (Mean Opinion Score) test. The proposed scheme provides 1.1 higher in Mean Opinion Score value and 5.61 dB higher than AMR - 12.2 kbit/s in terms of SNR in 10% packet loss, and maintains the communicab1e quality speech at frame erasure rates lop to 20%.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.39 no.11
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• pp.14-23
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• 2002

Two implementation methods for SOVA (Soft Output Viterbi Algorithm)of Turbo decoder are applied and verfied. The first method is the combination of a trace back (TB) logic for the survivor state and a double trace back logic for the weight value in two-step SOVA. This architecure of two-setp SOVA decoder allows important savings in area and high-speed processing compared with that of one-step SOVA decoding using register exchange (RE) or trace-back (TB) method. Second method is adjusting the reliability value with a scaling factor between 0.25 and 0.33 in order to compensate for the distortion for a rate 1/3 and 8-state SOVA decoder with a 256-bit frame size. The proposed schemes contributed to higher SNR performance by 2dB at the BER 10E-4 than that of SOVA decoder without a scaling factor. In order to verify the suggested schemes, the SOVA decoder is testd using Xillinx XCV 1000E FPGA, which runs at 33.6MHz of the maximum speed with 845 latencies and it features 175K gates in the case of 256-bit frame size.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.9B
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• pp.1742-1754
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• 1999

In this paper, we propose a rate control scheme, using histogram based rate-distortion (R-D) estimation, which produces a consistent picture quality between consecutive frames. The histogram based R-D estimation used in our rate control scheme offers a closed-form mathematical model that enable us to predict the bits and the distortion generated from an encoded frame at a given quantization parameter (QP) and vice versa. The most attractive feature of the R-D estimation is low complexity of computing the R-D data because its major operation is just to obtain a histogram or weighted histogram of DCT coefficients from an input picture. Furthermore, it is accurate enough to be applied to the practical video coding. Therefore, the proposed rate control scheme using this R-D estimation model is appropriate for the applications requiring low delay and low complexity, and controls the output bit-rate ad quality accurately. Our rate control scheme ensures that the video buffer do not underflow and overflow by satisfying the buffer constraint and, additionally, prevents quality difference between consecutive frames from exceeding certain level by adopting the distortion constraint. In addition, a consistent considering the maximum tolerance BER of the voice service. Also in Rician fading channel of K=6 and K=10, considering CLP=$10^{-3}$ as a criterion, it is observed that the performance improment of about 3.5 dB and 1.5 dB is obtained, respectively, in terms of $E_b$/$N_o$ by employing the concatenated FEC code with pilot symbols.

• The Journal of Korean Society of Virology
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• v.30 no.1
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• pp.71-81
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• 2000

We have determined and analyzed the full-length cDNA sequence of a coxsackievirus B3 (CVB3) Korean isolate (CVB3-Korea/97) which has been known as a general human pathogen. The whole genome contains 7,400 nucleotides and has a single large open reading frame with 6,555 nucleotides that encodes a potential polyprotein precursor of 2,185 amino acids. The genome also contains a 5' non-coding region (NCR) of 741 bases and a 3' NCR of 104 bases followed by poly(A) tail. Sequence homologies of nucleotides and deduced amino acids between the CVB3-Korea/97 strain and the prototype (Nancy strain) were 81.7% and 91.5%, respectively. The genes encoding the functional proteins including viral protease and RNA dependent RNA polymerase showed higher homology than those encoding the structural proteins. We have further analyzed the sequences of 5' NCR, VP1 and VP2 of CVB3-Korea/97, which are known as cardiovirulent determining factors at the nucleotide and amino acid levels. Although the CVB 3-Korea/97 strain was isolated from an aseptic meningitis patient without cardiomyopathy, its 234th nucleotide and 165th amino acid were uracil and Asn as same as those of other cardiovirulent strains one. However, the 155th amino acid of VP1, which closely associated with cardiovirulence, was replaced with $Arg^{155}$ by single nucleotide substitution from $A^{2916}$ to $T^{2916}$. Moreover, additional amino acid substitutions were observed in the flanking region of $Asp^{155}$. Taken together, amino acid(s) substitution in VP1 may playa critical role in determining cardiovirulence of the CVB3-Korea/97 strain rather than individual nucleotide replacements in the 5' NCR and/or an amino acid substitution in VP2.