• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.26 no.6
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• pp.620-627
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• 2000

The purpose of this study is to investigate the clinical and histologic changes in distraction osteogenesis according to different distraction rates in the rat's tibia. Eighteen adult rats underwent open osteotomy and attachment of an external unilateral distraction device in the middle of left tibia. Latency was allowed for 7 days before distracton began. The distraction device was activated with varying distraction rates of 0.5mm, 1mm, 2mm and same rhythm of twice a day until 5mm length gain was achieved. The animals were sacrificed at post-distraction 4, 8 weeks to observe the bony healing states. At each group, clinical, radiographic and histologic studies were done. The results obtained from this study were as follows: 1. The 0.5mm group showed excellent osteogenesis than other groups. The new bone was formed by intramembranous bone formation mostly and endochondral bone formation partly. 2. The 1mm group showed delayed osteogenesis and incomplete bony healing at 8 weeks. 3. The 2mm group showed weak osteogenesis and fibrous union or nonunion at 8 weeks. From these results, it could be stated that distraction rate of 0.5mm per day was most useful in rat's tibia. The rate of 1mm showed delayed bony healing and needed more consolidation period. Distraction osteogenesis is a excellent clinical method for regenerating local bone deficiencies in limbs and craniofacial area. The more studies needed for the higher animals and human about distraction rates and other biomechanical factors on the basis of this study.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.35 no.1
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• pp.7-12
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• 2009

Purpose: Gelatin-hydroxyapatite nanocomposite is similar to inorganic nanostructure of bone. To make a scaffold with osteoinductivity, bone marrow derived stem cells from rabbit femur were impinged into the nanocomposite. This vitro study was to test osteogenic differentiation of the stem cells in the nanocomposite, which was made by authors. Material & Methods: Gel-HA nanocomposite with 10g of HA, 3 g of Gel has been made by co-precipitation process. Bone marrow was obtained from femur of New Zealand White rabbits and osteogenic differentiation was induced by culturing of the BMSCs in an osteogenic medium. The BMSCs were seeded into the Gel-HA nanocomposite scaffold using a stirring seeding method. The scaffolds with the cells were examined by scanning electron microscopy (SEM), colorimetry assay, biochemical assay with alkaline phosphatase (ALP) diagnostic kit, osteocalcin ELISA kit. Results: Gel-HA nanocomposite scaffolds were fabricated with relatively homogenous microscale pores ($20-40{\mu}m$). The BMSCs were obtained from bone marrow of rabbit femurs and confirmed with flow cytometry, Alizarin red staining. Attachment and proliferation of BMSCs in Gel-HA nanocomposite scaffold could be identified by SEM, ALP activity and osteocalcin content of BMSCs. Conclusion: The Gel-HA nanocomposite scaffold with micropores could be fabricated and could support BMSCs seeding, osteogenic differentiation.

• Korean Journal of Childcare and Education
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• v.10 no.5
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• pp.273-298
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• 2014

The purpose of the study was to examine the image types of the traditional child rearing by mothers with infancy and early childhood through Q-methodology. To conduct that, Q-group was first formed from mothers who raised their child in traditional way after their brainstorming about the method and interviewing them. Based on the subject's shared statements, Q-standard was made with 40 subjects. The 40 subjects were asked to grade each statement from one to nine and then their responses were analyzed by QUANL program. The image types were classified into 4 groups. There were 'Type 1: Relationship-oriented parenting through harmony' 'Type 2: Archetypal Parenting based on the Korean traditional values,' 'Type 3: Practice-oriented Parenting to respect life,' and 'Type 4: Attachment Parenting modern variants necessary.' This study serves as a momentum to see the impact of traditional child rearing on modern child upbringing by examining mother's viewpoints on traditional child rearing.

• Child Health Nursing Research
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• v.20 no.4
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• pp.350-358
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• 2014

Purpose: The purpose of this study was to define and clarify the concept of developmental care for preterm infants. Methods: The hybrid model method was used to identify the main attributes and indicators. In the field work stage, data were collected in Seoul and Cheonan, Korea. The participants were 5 nurses working in the NICU. Results: The concept of developmental care was found to have six attributes and ten indicators in 2 dimensions. For the nursing practice dimension, four attributes were derived. They were being like intrauterine state, individualization, interaction, and integrative care with awareness of development. For the family centeredness dimension, supporting parental attachment and professional alliance were attributes of developmental care. Conclusion: Developmental focused care can facilitate the identification of behavioral responses of newborns and provide individualized interventions for fostering growth and development. This concept analysis could provide guidelines for "developmental care" nursing practice and be useful for research in the neonatal field.

• Journal of Technologic Dentistry
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• v.23 no.2
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• pp.75-93
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• 2002

This research was preformed for the purpose of preparing the items of standard model of the national dental technician test base on the duty analysis of the dental technician. The results of the duty analysis for the dental technician follows. 1. The dental technician is a profession to make the oral function smooth through the dental supplement and equipment in a scientific method and the skilled technique. 2. The duty of the dental technician are determined as A. preparation for manufacture B. manufacture C. management of the place of the dental technology D. self-development. A. The field of "the preparation for manufacture" are determined as 1. to confirm work authorization 2. To confirm the working model, B. The field of "In manufacture" are determined as 1. to manufacture the temporary crown 2. to manufacture the inlay and crown & bridge prosthesis 3. to manufacture the porcelain fused metal crown prosthesis 4. to manufacture the all ceramic crown prosthesis 5. to manufacture the temporary denture prosthesis 6. to manufacture the partial denture prosthesis 7. to manufacture the complete denture prosthesis 8. to manufacture the attachment prosthesis 9. to manufacture implant prosthesis 10. to manufacture the removable orthodontic device, 11. to manufacture the fixed orthodontic device, 12. to manufacture the orthodontic study cast C. The field of "in management of the dental lab." are determined as 1. management 2. to control the dental lab. D. The field of "In the self-development" are determined as 1. to improve the professionalism 2. self-control. 3. The developing items selected under the duty evaluation of the dental technician are l7s in the manufacture preparation, 1,011s in the manufacture, 7s in the management for the dental technology, 5s in self-development, and in all together 1,040s

• Journal of Korean Academy of Nursing
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• v.27 no.3
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• pp.588-600
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• 1997

Postpartum depression is one of the most serious problems in maternal health because it affects not only the mother but also her family. Postpartum depression disturbs the maternal-infant interaction and attachment. However, most postpartum depression patients ignore this problem and do not seek treatment. Many clinicians and researchers realiza there is a need to develop a postpartum depression scale. Thus, this study has been designed to development of a postpartum depression scale. Data were collected through a survey over a period of three months. Subjects who participated in the study were 167 Korean mothers in their postpartum period. The author used a convenience sampling method. The analysis of the data was done with SPSS PC/sup ＋/ for descriptive statistics, item analysis and factor analysis. Initially 62 items were generated from the interview data of eight postpartum depression patients and from a literature review. This preliminary scale was analyzed for reliability and validity. The results of this analysis are as follows. 1. Initially 62 items were analyzed through the Index of Content Validity(CVI) and 48 items were selected. 2. Seven factors were extracted through the principal component analysis, and these contributed 61% of the variance in the total score. Finally 46 items in the scale loaded .41∼ .84 on one of seven factors. 3. Each factor was labeled. Factor 1 was labeled 'emotional phenomena-emotional upset' and included 13 items, factor 2 was labeled' cognitive phenomena-self concept disturbance' and included seven items, factor 3 was labeled 'relationship to baby-negative feeling' and included six items, factor 4 was labeled 'relationship to baby- overload' and included eight items, factor 5 was labeled 'negative maternal identity' and included five items, factor 6 was labeled 'biophysiological phenomena-disturbance of physical functioning' and included four items, and factor 7 was labeled' interpersonal relationship phenomena-blamed others' and included three items. 4. Cronbach Coefficient Alpha for internal consistency was .95 for the total 46 items. Finally, the author suggests that this scale could be adequately applied in assessing the postpartum depression of mothers during the postpartum period. The results of this study can contribute to designing an appropriate postpartum depression prevention strategy.

• Journal of Korean Dental Science
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• v.9 no.1
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• pp.19-27
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• 2016

Purpose: Laser treatment has become a popular method in implant dentistry, and lasers have been used for the decontamination of implant surfaces when treating peri-implantitis. This study was performed to evaluate the effects of an Erbium-doped:Yttrium-Aluminum-Garnet (Er:YAG) laser with different settings on machined (MA), sand-blasted and acid-etched (SA), and resorbable blast media (RBM) titanium surfaces using scanning electron microscopy and confocal microscopy. Materials and Methods: Four MA, four SA, and four RBM discs were either irradiated at 40 mJ/20 Hz, 90 mJ/20 Hz, or 40 mJ/25 Hz for 2 minutes. The specimens were evaluated with scanning electron microscopy and confocal microscopy. Result: The untreated MA surface demonstrated uniform roughness with circumferential machining marks, and depressions were observed after laser treatment. The untreated SA surface demonstrated a rough surface with sharp spikes and deep pits, and the laser produced noticeable changes on the SA titanium surfaces with melting and fusion. The untreated RBM surface demonstrated a rough surface with irregular indentation, and treatment with the laser produced changes on the RBM titanium surfaces. The Er:YAG laser produced significant changes on the roughness parameters, including arithmetic mean height of the surface (Sa) and maximum height of the surface (Sz), of the MA and SA surfaces. However, the Er:YAG laser did not produce notable changes on the roughness parameters, such as Sa and Sz, of the RBM surfaces. Conclusion: This study evaluated the effects of an Er:YAG laser on MA, SA, and RBM titanium discs using confocal microscopy and scanning electron microscopy. Treatment with the laser produced significant changes in the roughness of MA and SA surfaces, but the roughness parameters of the RBM discs were not significantly changed. Further research is needed to evaluate the efficiency of the Er:YAG laser in removing the contaminants, adhering bacteria, and the effects of treatment on cellular attachment, proliferation, and differentiation.

• The Journal of Korean Academy of Prosthodontics
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• v.39 no.4
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• pp.336-350
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• 2001

The purpose of this study was to investigate corrosion tendency and to compare corrosion resistance of three dental magnetic attachments and its keeper alloy by coercive, electrochemical method. By using petentiodynamic polarization technique, magnetic elements and its keeper alloy of Magfit EX600 system(MF, MFK), Dyna ES regular system(DN, DNK) and Shiner SR magnet system(SR, SRK) were corroded electrochemically in 0.9% NaCl electrolytic solution. Open-circuit potential and anodic polarization curve was measured with Potentiostat(model 273 EG&E) and polarization curve was created by current density per square area following scanning of increased series of voltage in the rate of 1.0mV per second. Before and after electrochemical corrosion, the surface roughness test was done. Thereafter the change of mean surface roughness value(Ra) and mean peak value(Rt) of surface roughness was compared one another. In order to observe the corroded surface of each specimen, metallurgical light microscopic(${\times}37.5$) and scanning electron microscopic view(SEM ${\times}100$) was taken and compared one another. Conclusion is followings. 1. All of six covering metal of dental magnetic attachments and its keeper alloy were corroded in various degree after electrochemical corrosion. 2. The corrosion resistance of which used in this experiment is the following in high order; DNK, MFK, DN, MF, SRK and SR. 3. Especially Shiner magnet system and its keeper alloy were more severely corroded after electrochemical corrosion and the change of Ra Rt value were more increased than others. 4 Metallurgical and scanning electron microscopic view showed the pitting corrosion tendency of all experimental alloy but DNK and SR. 5. Covering metal of magnet was more corroded than its keeper alloy.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.11
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• pp.1659-1676
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• 2002

Ruminant animals develop a diverse and sophisticated microbial ecosystem for digesting fibrous feedstuffs. Plant cell walls are complex and their structures are not fully understood, but it is generally believed that the chemical properties of some plant cell wall compounds and the cross-linked three-dimensional matrix of polysaccharides, lignin and phenolic compounds limit digestion of cell wall polysaccharides by ruminal microbes. Three adaptive strategies have been identified in the ruminal ecosystem for degrading plant cell walls: production of the full slate of enzymes required to cleave the numerous bonds within cell walls; attachment and colonization of feed particles; and synergetic interactions among ruminal species. Nonetheless, digestion of fibrous feeds remains incomplete, and numerous research attempts have been made to increase this extent of digestion. Exogenous fibrolytic enzymes (EFE) have been used successfully in monogastric animal production for some time. The possibility of adapting EFE as feed additives for ruminants is under intensive study. To date, animal responses to EFE supplements have varied greatly due to differences in enzyme source, application method, and types of diets and livestock. Currently available information suggests delivery of EFE by applying them to feed offers the best chance to increase ruminal digestion. The general tendency of EFE to increase rate, but not extent, of fibre digestion indicates that the products currently on the market for ruminants may not be introducing novel enzyme activities into the rumen. Recent research suggests that cleavage of esterified linkages (e.g., acetylesterase, ferulic acid esterase) within the plant cell wall matrix may be the key to increasing the extent of cell wall digestion in the rumen. Thus, a crucial ingredient in an effective enzyme additive for ruminants may be an as yet undetermined esterase that may not be included, quantified or listed in the majority of available enzyme preparations. Identifying these pivotal enzyme(s) and using biotechnology to enhance their production is necessary for long term improvements in feed digestion using EFE. Pretreating fibrous feeds with alkali in addition to EFE also shows promise for improving the efficacy of enzyme supplements.

• Journal of the Korean Chemical Society
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• v.37 no.11
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• pp.937-942
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• 1993

Enzyme electrodes for amperometric measurement of ammonia was prepared by immobilization of L-glutamate dehydrogenase on an Immobilon-AV Affinity membrane and attachment to a glassy carbon electrode. Reduced nicotinamide adenine dinucleotide (NADH) was used as the electroactive species. The electrochemical oxidation of NADH was monitored at ＋1.0 volt vs. Ag/AgCl. Response was linear from $4.0\;{\times}\;10^{-5}\;to\;4.0\;{\times}\;10^{-4}$ M. The detection limit was 2.0 ${\times}\;10^{-6}$ M. Response time, the optimum pH and life time of enzyme immobilized membrane were 2 min, pH 7.3∼7.6 (Dulbecco's buffer solution) and about 25 days respectively. When the enzyme electrode was applied to the $NH_4^+$ determination with amperometric method, other physiological materials had no interference.