$NH_4^+$ 정량을 위한 Amperometric Enzyme Electrode

Amperometric Enzyme Electrode for the Determination of $NH_4^+$

  • 서무룡 (경상대학교 자연과학대학 화학과) ;
  • 김재상 (경상대학교 자연과학대학 화학과) ;
  • 이심성 (경상대학교 자연과학대학 화학과) ;
  • 배준웅 (경북대학교 자연과학대학 화학과) ;
  • 이흥락 (경상대학교 자연과학대학 화학과) ;
  • 박태명 (순천공업전문대학 환경공업과)
  • Moo Lyong Seo (Department of Chemistry, Gyeongsang National University) ;
  • Jae Sang Kim (Department of Chemistry, Gyeongsang National University) ;
  • Shim Sung Lee (Department of Chemistry, Gyeongsang National University) ;
  • Zun Ung Bae (Department of Chemistry, Kyungpook National University) ;
  • Heung Lark Lee (Department of Chemistry, Gyeongsang National University) ;
  • Tae Myung Park (Department of Environmental, Sooncheon Junior Technical College)
  • 발행 : 1993.11.20

초록

Immobilon-AV Affinity membrane에 L-glutamate dehydrogenase를 고정하여 유리질 탄소전극에 부착시킨 전극을 사용하여 암모니아를 전류법으로 정량하였다. 이때 환원형의 NADH가 $NAD^+$로 산화될 때 전류를 +1.0 volt vs. Ag/AgCl에서 측정하였다. 효소 고정화된 membrane을 부착시킨 전극의 감응 특성은 다음과 같다. 곧 직선 감응 농도 범위는 $4.0\;{\times}\;10^{-5}\;{\sim}\;4.0\;{\times}\;10^{-4}$ M이었으며, 정량한계는 2.0 ${\times}\;10^{-6}$ M이었다. 또한 감응 시간은 2분이었으며 효소 고정화된 막의 최적 pH와 수명은 각각 pH 7.3∼7.6 (Dulbecco's buffer 용액)과 25일이었다. 그리고 다른 생리활성 물질의 방해는 없었다.

Enzyme electrodes for amperometric measurement of ammonia was prepared by immobilization of L-glutamate dehydrogenase on an Immobilon-AV Affinity membrane and attachment to a glassy carbon electrode. Reduced nicotinamide adenine dinucleotide (NADH) was used as the electroactive species. The electrochemical oxidation of NADH was monitored at +1.0 volt vs. Ag/AgCl. Response was linear from $4.0\;{\times}\;10^{-5}\;to\;4.0\;{\times}\;10^{-4}$ M. The detection limit was 2.0 ${\times}\;10^{-6}$ M. Response time, the optimum pH and life time of enzyme immobilized membrane were 2 min, pH 7.3∼7.6 (Dulbecco's buffer solution) and about 25 days respectively. When the enzyme electrode was applied to the $NH_4^+$ determination with amperometric method, other physiological materials had no interference.

키워드

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