• Korean Journal of Food Science and Technology
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• v.49 no.1
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• pp.90-96
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• 2017

The aim of this study was to investigate the anti-inflammatory and ultraviolet (UV)-protective effect of Pleurotus eryngii extract (PEE) in activated RAW 264.7 cells and UV-induced mouse skin damage. The results showed that PEE strongly inhibited the production of inflammatory mediators such as nitric oxide, interleukin $(IL)-1{\beta}$, and IL-6 at high concentrations in LPS-stimulated RAW 264.7 macrophages. In addition, PEE treatment suppressed erythema, melanin index, and epidermal thickness to a greater degree than ascorbic acid (AA) treatment in UV-irradiated mice. Finally, PEE treatment inhibited the infiltration of mast cell to a similar degree of AA treatment. Therefore, these results indicate that PEE could improve inflammation and skin damage in immune cells and UV-irradiated mice. This study may provide positive insights into PEE as a functional food and cosmetic ingredient for treatment of inflammation and skin damage.

• Korean Journal of Food Science and Technology
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• v.48 no.6
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• pp.582-589
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• 2016

The aim of this study was to investigate the synergistic anti-inflammatory and anti-dermatitis effects of grape branch extract (GBE) and Pleurotus eryngii (PEE) combinations on the active immune cells and atopic dermatitis-like skin lesions in mice. The results showed that the combination of GBE ($12.5{\mu}g/mL$) and PEE ($500{\mu}g/mL$) led to much stronger inhibitory effects on the production of inflammatory mediators, such as NO and $PGE_2$ than that exhibited by GBE ($25{\mu}g/mL$) and PEE ($1000{\mu}g/mL$) alone, even at higher concentrations, in LPS-stimulated RAW 264.7 macrophages. The combination of GBE and PEE synergistically inhibited the production of TNF- and IL-6 in LPS-stimulated RAW 264.7 macrophages and PMA plus A23187-activated HMC-1 cells. Furthermore, combined GBE and PEE had a stronger ameliorative effect than GBE and PEE alone by inhibiting the clinical sores, IgE, and IL-4 on atopic dermatitis-like skin lesions in mice. Collectively, these results suggested that the combination of GBE and PEE produced a synergistic anti-inflammatory and anti-atopic dermatitis effect on immune cells and atopic dermatitis-like skin lesions in mice.

• Korean Journal of Pharmacognosy
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• v.45 no.3
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• pp.220-226
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• 2014

Diospyros lotus has been cultivated for its edible fruits and leaves which are considered for its medicinal importance. The aim of this study was to evaluate the anti-melanogenesis of ethyl acetate (EA) fractions from D. lotus leaves in B16 cells. The order of the total polyphenol content with regard to the different solvent fractions from D. lotus leaves was EA>butanol>metahanol>chloroform>n-hexane. The major compounds of EA fraction from D. lotus leaves by HPLC analysis were myricitrin and myricetin. Cellular TYR activity and melanin content in response to treatment with 100 mg/mL of EA fraction was inhibited more strongly than group treated with arbutin. Further, EA fraction exhibited significant anti-melanogenesis effects by reducing the levels of microphthalima-associated transcription factor (MITE), inhibiting the synthesis of TYR, tyrosinase-related protein-1 (TRP-1) and TRP-2. Therefore, EA fractions from D. lotus leaves may be a good source of skin-whitening agents in the future development of medicine-based trouble skin therapy.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.11
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• pp.1635-1641
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• 2014

Diospyros lotus has been cultivated for its edible fruits, which are considered to have medicinal importance. The aim of this study was to evaluate the antioxidant and antipruritic activities of water-soluble, methanol extract, and ethyl acetate (EA) fractions from D. lotus leaves. The EA fraction showed the lowest $IC_{50}$ vale (DPPH: $5.3{\mu}g/mL$, ABTS: $53.8{\mu}g/mL$). Therefore, we further investigated anti-inflammatory and antipruritic effects of the EA fraction. TNF-${\alpha}$ production increased by PMA plus A23187 treatment was significantly inhibited by the EA fraction in a dose-dependent manner. The EA fraction also inhibited histamine release from rat peritoneal mast cells stimulated by compound 48/80, which promotes histamine release. Furthermore, EA fraction had inhibitory effects on scratching behavior induced by compound 48/80 in Balb/c mice. These results suggest that the EA fraction from D. lotus leaves has potential as ameliorative agent against oxidative stress and pruritus-related disease.

• Electronics and Telecommunications Trends
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• v.27 no.5
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• pp.134-149
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• 2012

본고에서는 차세대 스마트 인프라 준거틀로서 '만물지능통신 기반 초연결 인프라' 개념을 제시한다. 동 개념이 대두하게 된 근거 기반으로 기술적 관점에서 스마트 기술혁신과 인프라 혁신 간의 상호관계성, 문명사적 관점에서 정보 교통 에너지 인프라 간의 공진화 과정을 거시적으로 개관한다. 그리고 초연결 클라우드 컴퓨팅, 만물지능통신망과의 선순환 관계에서 기존 인프라의 고도화와 동시에 정전교(情電交) 초연결 인프라의 탄생과 발전 방향을 모색한다. 또한 EU의 중장기 R&D 전략의 공통성과 도출을 위한 분산화된 협업시스템으로 부상하고 있는 SoS(System of Systems) 모델을 초연결 인프라의 프로토타입으로 검토한다. 이러한 작업을 기반으로 '물리적 인프라의 추상화' 그리고 '추상화한 인프라의 현실 세계로의 실체화'라는 관점에서 초연결 인프라로의 재편 메커니즘을 분석하고, 초연결 인프라를 지향한 공통 플랫폼과 그 실현을 위한 기술적 정책적 함의를 도출한다.

• Electronics and Telecommunications Trends
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• v.27 no.4
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• pp.40-53
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• 2012

통신은 기본적으로 사람과 사람 간의 의사소통에서 출발한다. 그러나 만물지능통신은 통신의 대상을 사람-사물-공간-시스템으로 확장한 초연결(hyperconnectivity) 네트워킹을 전제로 한다. 본고에서는 이러한 만물지능통신 기반의 초연결 산업 구도를 분석하기 위하여, 스마트 혁명 이후의 IT 산업 분석틀로 부상한 CPNT(Content, Platform, Network, Terminal) 계층구조를 적용함과 동시에 초연결 산업의 준거틀로 일본 경제산업성 기술전략맵에 포함된 미래 사회의 삽화 내용을 원용했다. 동 기술전략맵에서는 2025년을 실현연도로 상정하여, 기술이 개발된 미래 생활환경을 삽화를 통하여 기술하고 있다. 동 삽화의 구도와 내용을 분석한 결과 초연결 산업은 사람-사물-공간-시스템 간의 초연결로 특징지을 수 있고, 콘텐츠-플랫폼-단말-네트워크 계층구조로 재구성할 수 있음을 확인할 수 있었다. 이러한 작업을 통하여 초연결 산업 생태계의 기본 구도로서 천지인(天地人) 모델을 제안하였다.

• Proceedings of the Korea Information Processing Society Conference
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• 2013.05a
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• pp.806-808
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• 2013

야간에 항로를 알려주는 등명기는 항상 점등 되어 있어야 한다. 따라서 중요한 장소에는 주 등명기 뿐만 아니라 예비 등명기를 추가 설치 운영해야한다. 이는 추가적인 경제적 비용 및 설치공간부족 등 많은 제약조건이 따르게 된다. 하지만 연구 중인 예비기능을 구비한 등명기가 개발되면 이러한 문제점을 해결할 수 있다. 본 논문에서는 예비기능을 구비한 등명기와 통신을 하기 위한 프로토콜을 제안한다.

• Proceedings of the Korea Information Processing Society Conference
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• 2013.05a
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• pp.802-805
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• 2013

현재 국내에서 운용되는 해상용 항로표지는 기존 제품 보다 활용성이 크게 향상되었고, LED전구를 적용하여 운용기간도 연장되었으나, 고장시 대처방법이 없으며, 유지보수에 추가적인 비용이 발생하고 있다. 이와 관련하여 본 논문에서는 항로표지에 주광원과 예비광원을 동시에 형성하여 주광원 고장시 예비광원으로 운용이 가능한 LED 해상용 항로표지에 적용되는 제어회로를 개발하고 구현 하였다.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.112.3-113
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• 2003

More than 30 isolates of Alternaria were obtained from various solanaceous crops in Korea. For all isolates, morphological characteristics of the conidia were determined and compared with those of representative isolates of A. solani and A. tomatophila. Pathogenicity test was performed to Potato, tomato, egg plant and red Pepper and molecular characteristics of them including the representative isolates were determined using sequence analyses of ITS rDNA and histone H3 gene, and URP-PCR analysis. Based on morphological characteristics, the isolates from the solanaceous crops were grouped as identical or very similar to either A. tomatophila(ATO), A. solani(ASO), and unidentified Altemaria sp.(ASP). Among the molecular markers used in this study, the URP-PCR analysis was found to be appropriate for taxonomic resolution of these species. Based on the conidial morphology, pathogenicity test and molecular characteristics, A. tomatophila(early blight of tomato) could be distinguished from A. solani(early blight of potato), and the Alternaria sp.(ASP) from potato, which was closely related to A. solani in conidial morphology, was considered as a new species.

• Journal of Pharmaceutical Investigation
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• v.40 no.6
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• pp.373-378
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• 2010

In order to develop a topical preparation which has a high occlusive property with skin moisturization, nano-structured lipid carrier (NLC) systems along with solid lipid nanoparticle (SLN) were designed. Various NLC dispersions were successfully formulated with Compritol 888 ATO as a solid lipid, Labrafil M 1944 CS as an oil, and Tween 80 as a surfactant. The increase of oil content (5 to 50%) led to the decrease in the occlusion factor in the order of SLN > NLC-5 > NLC-15 = NLC-30 > NLC-50. Particle size of lipid particulates was in the range of 100 to 160 nm. NLC-based carbogels were prepared by the employment of humectants such as urea, glycerin, and Tinocare GL to carbomer gel. NLC-30 gel formulations containing 4 or 8 % of lipid particles showed improved occlusive effect in vitro, compared to NLC-free gel base. Even though NLC-free gel base revealed comparable occlusion effect by itself, the occlusion factor of 4 % NLC-30 gel was about 2-fold higher than that of NLC-free gel base.