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http://dx.doi.org/10.3746/jkfn.2014.43.11.1635

Antioxidant and Antipruritic Activities of Ethyl Acetate Fraction from Diospyros lotus Leaves  

Jeon, In Hwa (Ato Q&A Incoperation)
Kang, Hyun Ju (Ato Q&A Incoperation)
Kim, Sang Jun (Jeonju Biomaterials Institute)
Jeong, Seung Il (Jeonju Biomaterials Institute)
Lee, Hyun-Seo (Department of Healthcare & Science, Jeonju University)
Jang, Seon Il (Ato Q&A Incoperation)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.43, no.11, 2014 , pp. 1635-1641 More about this Journal
Abstract
Diospyros lotus has been cultivated for its edible fruits, which are considered to have medicinal importance. The aim of this study was to evaluate the antioxidant and antipruritic activities of water-soluble, methanol extract, and ethyl acetate (EA) fractions from D. lotus leaves. The EA fraction showed the lowest $IC_{50}$ vale (DPPH: $5.3{\mu}g/mL$, ABTS: $53.8{\mu}g/mL$). Therefore, we further investigated anti-inflammatory and antipruritic effects of the EA fraction. TNF-${\alpha}$ production increased by PMA plus A23187 treatment was significantly inhibited by the EA fraction in a dose-dependent manner. The EA fraction also inhibited histamine release from rat peritoneal mast cells stimulated by compound 48/80, which promotes histamine release. Furthermore, EA fraction had inhibitory effects on scratching behavior induced by compound 48/80 in Balb/c mice. These results suggest that the EA fraction from D. lotus leaves has potential as ameliorative agent against oxidative stress and pruritus-related disease.
Keywords
Diospyros lotus leaf; ethyl acetate fraction; antioxidant; mast cells; antipruritus;
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