• Journal of Life Science
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• v.21 no.9
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• pp.1295-1300
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• 2011

The AtPGR gene is induced by pathogen infection, jasmonic acid and salicylic acid treatment and may therefore play a role in plant defense responses. Arabidopsis thaliana Plasma membrane Glucose-responsive Regulator (AtPGR) was previously isolated from Arabidopsis, which confers glucose insensitivity on plants. To study its biological functions directly, we have characterized both loss-of-function RNAi mutant and gain-of-function transgenic overexpression plants for AtPGR in Arabidopsis. The AtPGR-overexpressing plants displayed enhanced resistance to a virulent strain of the bacterial pathogen Pseudomonas syringae as measured by a significant decrease in both bacterial growth and symptom development as compared to those in wild-type and RNAi plants. The enhanced resistance in the gain-of-function transgenic plants was associated with increased induction of SA-regulated PDF1.2 and JA-regulated PR1 by the bacterial pathogen. Thus, pathogen-induced AtPGR plays a positive role in defense responses to P. syringae.

• Development and Reproduction
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• v.23 no.3
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• pp.277-284
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• 2019

ARID1A and PGR plays an important role in embryo implantation and decidualization during early pregnancy. Uterine specific Arid1a knockout ($Pgr^{cre/+}Arid1a^{f/f}$) mice exhibit in non-receptive endometrium at day 3.5 of gestation (GD 3.5). In previous studies, using transcriptomic analysis in the uterus of $Pgr^{cre/+}Arid1a^{f/f}$ mice, we identified proline-rich acidic protein 1 (PRAP1) as one of the down-regulated genes by ARID1A in the uterus. In the present study, we performed RT-qPCR and immunohistochemistry analysis to investigate the regulation of PRAP1 by ARID1A and determine expression patterns of PRAP1 in the uterus during early pregnancy. During early pregnancy, PRAP1 expression was strong at day 0.5 of gestation (GD 0.5) and then decreased at GD 3.5 in the epithelium and stroma. After implantation, PRAP1 expression was remarkably reduced in the uterus. However, the expression of PRAP1 at GD 3.5 was remarkably increased in the $Pgr^{cre/+}Arid1a^{f/f}$ mice. To determine the ovarian steroid hormone regulation of PRAP1, we examined the expression of PRAP1 in ovariectomized control, $Pgr^{cre/+}Arid1a^{f/f}$, and progesterone receptor knock-out (PRKO) mice treated with progesterone. While PRAP1 proteins were strongly expressed in the luminal and glandular epithelium of control mice treated with vehicle, progesterone treatment suppressed the expression of PRAP1. However, PRAP1 was not suppressed in both the $Pgr^{cre/+}Arid1a^{f/f}$ and PRKO mice compared to controls. Our results identified PRAP1 as a novel target of ARID1A and PGR in the murine uterus.

• The Journal of Korean Obstetrics and Gynecology
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• v.31 no.4
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• pp.1-15
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• 2018

Objectives: Osteoporosis is considered a serious human disease. We developed an extract of mixed herbs containing root of Platycodon grandiflorum (ExMH-PGR), which is expected to be effective in preventing or treating osteoporosis. The aim of this study was to investigate the anti-osteoporotic effect of ExMH-PGR in osteoblastic MC3T3-E1 cells and osteoclastic RAW 264.7 cells. Methods: To examine the anti-osteoporotic effect of ExMH-PGR, osteoblast and osteoclast differentiation were induced and cultured with various concentrations of ExMH-PGR. Alkaline phosphatase (ALP) activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells were analyzed. Tartrate-resistant acid phosphatase (TRAP) activity and the formation of actin ring in RAW 264.7 cells were analyzed. Results: ExMH-PGR at concentration up to $25{\mu}g/mL$ significantly increased ALP activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells. ExMH-PGR at 50 to $200{\mu}g/mL$ significantly inhibited TRAP activity and the formation of actin ring in RAW 264.7 cells. Conclusions: These results demonstrate that ExMH-PGR stimulates osteoblastic activities and inhibits osteoclastic activities in in vitro systems, suggesting that ExMH-PGR might be considered as an anti-osteoporotic candidate for treatment of osteoporosis disease.

• Animal Bioscience
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• v.37 no.8
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• pp.1377-1386
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• 2024

Objective: Embryonic interferon-tau (IFNT) and progesterone affect expression of interferon-stimulated genes (ISGs), progesterone receptor (PGR) and progesterone-induced blocking factor (PIBF) in the ovine thyroid. Methods: Thyroids of ewes were sampled at day 16 of nonpregnancy, days 13, 16, and 25 of pregnancy, and real-time quantitative polymerase chain reaction assay, western blot and immunohistochemistry were used to detect expression of ISGs, PGR, and PIBF. Results: Free ISG15 protein was undetected, but ISG15 conjugated proteins upregulated at day 16 of pregnancy, and expression levels of ISG15 conjugated proteins, PGR isoform (70 kDa), PIBF, interferon-gamma-inducible protein 10 and myxovirusresistance protein 1 peaked, but expression level of signal transducer and activator of transcription 1 was the lowest at day 16 of pregnancy. In addition, the expression levels of PGR isoform (70 kDa) and signal transducer and activator of transcription 1 (STAT1) decreased, but levels of PGR isoform (43 kDa), 2',5'-oligoadenylate synthetase, IP-10 and MX1 increased at day 25 of pregnancy comparing with day 16 of the estrous cycle. Conclusion: Early pregnancy affects expression of ISGs, PGR, and PIBF in maternal thyroid through IFNT and progesterone, which may regulate thyroid autoimmunity and thyroid hormone secretion in ewes.

• Asian Journal of Turfgrass Science
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• v.3 no.1
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• pp.39-52
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• 1989

Native Kerean lawngrass (Zeysia japonica steud.) and Manilagrass ( Zeysia matrella Merr.) are frequently used as sport turf and lawn in Kerea and ether Asian countries because of its excellent summer qualities and superior winter-hardiness compared to ether warm-season turfgrass . The objective of this study was to investigate the effect of the plant growth retardants ( PGR ) en the inhibition (d the growth of the two Kerean native turfgrasses. Kerean lawngrass and Manilagrass were treated with [2 RS , 3 RS] -1- [4- chlorophenyl ] -4, 4-dimethyl -2- [1 H -1, 2, 4- tiazol -1- y] pentan -3- ol ( PP -333) at 93 and 930 gha -j ; 5-[4- chlorphenyl ]-3, 4, 5, 9, 10- peiitaaza - tetracyclo [5, 4, 1, 0 2 6 . o ~'~I -3, 9- diene ( BAS -106) at 16 and 160 gha ; [ E I-] - cyclohexyl -4, 4- dimethyl -2- [1, 2, 4- triazol -1- yl ]-1-pentene - 3- ol ( NTN -821) at 56 and 560 gha ', and 4- chloro -2- [$\alpha$- hydrozybenzy I isonicotin anilide ( CGR -811) at 92 gha in pot study . All PGR treatments were effective in reducing the plant height, with the exception of BAS -106 at 16 gha for Korean lawngrass ; however , all increased quality to some degree , with the exception of PP -333 at 93 gha . The low PGR rates significantly increased Korean lawngrass height during late jtily aitd mid-August . On the other hand , BAS -106 significantly increased Manilagrass height {rom 24 Septeml)er onwards . All PGR treatments haol higher turfgrass quality than untreatments , and also the high rates of PGIt treatments were more effective in increasing quality than the low rates observed on 8 October . Meanwhile , Manilagrass quality was increased to some extent than Korean lawegrass . PP - 333 was significantly effective in reducing clipping yield and the same results were found with the application of NTN -821 at 560 gha -l for both Korean lawngrass and Manilagrass , BAS -106 at 160 gha for Korean lawegrass , and CGR -811 at 92 gha for Manilagrass . The high PGR rates had higher turfgrass shoot weight observed on 8 October than the low rates , and there were little increases obtained with the low rates of PP -333 for Korean lawngrass and BAS - 1 06 for Manilagrass . Effeets on stolon growth varied between growth retardants , and between retardant treatments ; the high rates of PGR as well as the low rate of NTN -821 elongated stolon of Korean lawngrass and manoilagrass . However , PP -333 at 93 gha and BAS -106 at 16 gha - shortened stolons of Korean lawugrass , resulting in inhibition or little difference of stolon dry weight . NTN -821 at 560 gha and CGR -811 had no positive effect on the stolon length of Manilagrass . 101) -333 and the low rate of BAS -106 and NTN -821 decreased root weight of Korean lawng - rass All PGR rates had negative effect on the root weight of Manilagrass with the exception of BAS -106 CR -811 and the high rate of NTN -821 which made no difference in root weight between retarolant treatments and the control .

• Korean Journal of Pharmacognosy
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• v.36 no.3 s.142
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• pp.209-219
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• 2005

In this study, we carried out the preparing standardization and regulation of processed Glycyrrhizae Radix (PGR) which have been widely used in oriental medicines. Glycyrrhizae Radix(GR) have been generally prepared by the stir-frying, or mix-frying with honey for the purpose of decreasing sweetness and augmenting vitality. Firstly, we tried to standardize PGR prepared by the stir-frying. We purchased 14 kinds of PGR and non-processed GR(NPGR) at oriental physician's offices and oriental pharmacies on a nation scale, respectively. The amounts of dry on loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, glycyrrhizin(GL), glycyrrhetic acid(GA) and liquiritin(LQ) of them were examined. The amounts of dry on loss, GL and LQ in commercial PGRs showed remarkable decrease, while GA showed increased as compared with NPGR. In order to standardize preparing method of PGR, the effect of heating time on physico-chemical parameters and biological activities were examined. Physico-chemical parameters such as dry on loss, extract amount, GL and LQ contents in PGRs showed decrease, however, GA was increased with heating time as compared with NPGR. Also, GA, obtained from heat-treated GR, was found as an artifact in PGRs. PGR was more effective than NPGA in vitro test of DPPH scavenging effect and TBA-Rs reducing effect. PGR and NPGR showed potent hepatoprotective effect on $CCl_4-intoxicated$ rats. Especially, PGR prepared by 80 min of heating was the most effective. Considering these results, the optimal condition for PGR preparation was $150^{\circ}C$ for 80 min.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1176-1182
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• 2018

Objective: Progesterone receptor (PGR) and AT-rich interactive domain 1A (ARID1A) have important roles in the establishment and maintenance of pregnancy in the uterus. In present studies, we examined the expression of mitochondrial tumor suppressor 1 (MTUS1) in the murine uterus during early pregnancy as well as in response to ovarian steroid hormone treatment. Methods: We performed quantitative reverse transcription polymerase chain reaction and immunohistochemistry analysis to investigate the regulation of MTUS1 by ARID1A and determined expression patterns of MTUS1 in the uterus during early pregnancy. Results: The expression of MTUS1 was detected on day 0.5 of gestation (GD 0.5) and then gradually increased until GD 3.5 in the luminal and glandular epithelium. However, the expression of MTUS1 was significantly reduced in the uterine epithelial cells of $Pgr^{cre/+}Arid1a^{f/f}$ and Pgr knockout (PRKO) mice at GD 3.5. Furthermore, MTUS1 expression was remarkably induced after P4 treatment in the luminal and glandular epithelium of the wild-type mice. However, the induction of MTUS1 expression was not detected in uteri of $Pgr^{cre/+}Arid1a^{f/f}$ or PRKO mice treated with P4. Conclusion: These results suggest that MTUS1 is a novel target gene by ARID1A and PGR in the uterine epithelial cells.

• Korean Journal of Environmental Biology
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• v.34 no.4
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• pp.353-360
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• 2016

Effect of heavy metals(Cd, Cu, Zn) on the survival and population growth rates(PGR) of marine rotifer, Brachionus plicatilis were examined. B. plicatilis were exposed to Cd, Cu and Zn for 24 h to determine their survival and 72 h to determine their PGR. Survival rates in the control groups were greater than 90%. They were decreased with increasing concentrations of Cd, Cu and Zn. Survival rates were reduced in a concentration-dependent manner. Significant reduction in survival rates after exposure to Cd, Cu and Zn at concentration greater than 40.00, 0.13 and $10.00mg\;L^{-1}$, respectively. PGR in the control groups were greater than 0.50. They were decreased with increasing concentrations of heavy metals. PGR were reduced in a concentration-dependent manner. Significant reduction in PGR after exposure to Cd, Cu and Zn occurred at concentration greater than 12.5, 0.06 and $1.00mg\;L^{-1}$, respectively. The order of heavy metal toxicity based on PGR was Cu>Zn>Cd, with $EC_{50}$ (50% Effective Concentration) values of 0.12, 6.15 and $21.41mg\;L^{-1}$, respectively. The lowest-observed-effective-concentrations(LOEC) of PGR after exposure to Cd, Cu and Zn were 12.50, 0.06 and $1.00mg\;L^{-1}$, respectively. The No-observed-effective-concentrations(NOEC) of PGR after exposure to Cd, Cu and Zn were 6.25, 0.03 and $0.01mg\;L^{-1}$, respectively, in marine ecosystems have toxic effects on PGR of B. plicatilis. These results suggest that the PGR of B. plicatilis are useful tool to assess the effect of heavy metals on primary consumers in marine natural ecosystems.

• Journal of Ginseng Research
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• v.35 no.3
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• pp.261-271
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• 2011

Chemical radiation protection is an important strategy to protect living beings against the deleterious effects of radiation. In the present study, the radioprotective effect of hydro-alcoholic extract of Panax ginseng extract (PGR-HAE) was studied on radiation-induced deleterious alterations in Swiss albino mice. Oral administration of such extract (25 mg/kg b wt/day/animal) for 5 consecutive days, half an h. before whole-body exposure to 6 Gy gamma radiation, enhanced the 30 days survival and also inhibited the radiogenic sickness, weight loss and life shortening. PGR-HAE ameliorated radiation induced depletion in blood constituents at different necropsy intervals between 12 h to 30 d, and significantly increased the number of femoral spleen colony forming units that survived after irradiation. Furthermore, it checked depletion of glutathione and antioxidant enzymes (superoxide dismutase, catalase, and glutathione S-transferase) as well as elevation of lipid peroxidation (LPO) level in blood and liver. The significant reduction in the yield of LPO demonstrates that PGR-HAE protects the membranes against radiation-induced oxidative damage. These findings conclude that such plant extract provides significant radioprotection, and it may be potentially valuable in the prevention of injuries caused during planned and unplanned radiation exposure.

• Journal of Animal Science and Technology
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• v.44 no.3
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• pp.297-304
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• 2002

This study was conducted to investigate the influence of dietary Panax Ginseng Refuse(PGR), Dioscorea Japonica Refuse(DJR) and Oriental Medicine Refuse(OMR) on performance(feed intake, body weight gain, feed conversion, mortality) of Korean Native Chicken(KNC). KNC were randomly assigned to one of the 4 dietary treatment : 1) control(commercial feed), 2) PGR(commercial feed supplemented with 5% panax ginseng leaves) 3) DJR(commercial feed with 5% dioscorea japonica hulls and 4) OMR (commercial feed with 5% oriental medicine refuse). 160 KNC were fed one of the above experimental diet for 12 weeks and slaughtered at 20 weeks of age. Daily DM intake of control, PGR, DJR and OMR diet were 125.79g, 122.26g, 122.30g and 123.72g, respectively, with no significantly difference(p$>$0.05). The DM utilizability of control and DJR(62.89% and 61.20%) diets were higher(p$<$0.05) than those of PGR and OMR(55.44% and 59.76%)(p$<$0.05). 16 weeks weight of bird fed control, PGR, DJR and OMR diets were 1,436.6g, 1,427.2g, 1,546g and 1,422.0g respectively with DJR being the highest than other treatments(p$<$0.05). Total feed intake of control, PGR, DJR and OMR diets were 6,087g, 5,947g, 5,816g and 5,852g, respectively where control was higher than other treatments. The feed conversion of PGR, DJR and OMR were higher than the control. Chicken housed mortality of control, PGR, DJR and OMR were respectively 24, 15, 13 and 20 birds with rather lower mortality in the supplented groups(p$<$0.05). The body weight, carcass weight and carcass yield were not different among the treatments. But abdominal fat of chicken in control and OMR groups were higher than that of DJR and PGR groups.