Browse > Article

Studies on the Processing of Crude Drugs(IX) -Preparing Standardization and Regulation of Stir-Frying Glycyrrhzia root(1)-  

Choi, Hyuck-Jai (East-West Medical Research Institute, Kyung Hee University)
Lee, Woo-Jung (East-West Medical Research Institute, Kyung Hee University)
Park, Sung-Hwan (East-West Medical Research Institute, Kyung Hee University)
Song, Bo-Whan (East-West Medical Research Institute, Kyung Hee University)
Kim, Dong-Hyun (College of Pharmacy, Kyung Hee University)
Kim, Nam-Jae (East-West Medical Research Institute, Kyung Hee University)
Publication Information
Korean Journal of Pharmacognosy / v.36, no.3, 2005 , pp. 209-219 More about this Journal
Abstract
In this study, we carried out the preparing standardization and regulation of processed Glycyrrhizae Radix (PGR) which have been widely used in oriental medicines. Glycyrrhizae Radix(GR) have been generally prepared by the stir-frying, or mix-frying with honey for the purpose of decreasing sweetness and augmenting vitality. Firstly, we tried to standardize PGR prepared by the stir-frying. We purchased 14 kinds of PGR and non-processed GR(NPGR) at oriental physician's offices and oriental pharmacies on a nation scale, respectively. The amounts of dry on loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, glycyrrhizin(GL), glycyrrhetic acid(GA) and liquiritin(LQ) of them were examined. The amounts of dry on loss, GL and LQ in commercial PGRs showed remarkable decrease, while GA showed increased as compared with NPGR. In order to standardize preparing method of PGR, the effect of heating time on physico-chemical parameters and biological activities were examined. Physico-chemical parameters such as dry on loss, extract amount, GL and LQ contents in PGRs showed decrease, however, GA was increased with heating time as compared with NPGR. Also, GA, obtained from heat-treated GR, was found as an artifact in PGRs. PGR was more effective than NPGA in vitro test of DPPH scavenging effect and TBA-Rs reducing effect. PGR and NPGR showed potent hepatoprotective effect on $CCl_4-intoxicated$ rats. Especially, PGR prepared by 80 min of heating was the most effective. Considering these results, the optimal condition for PGR preparation was $150^{\circ}C$ for 80 min.
Keywords
Glycyrrhiza uralensis; herb processing; glycyrrhizin; glycyrrhetic acid; liquiritin; stir-flying;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kun-Ying Yen (1992) Relationship between syndrome pattern and the process of preparing Chinese medicines. 漢方藥. 代謝. 29(臨時增刊號): 108-116
2 홍문화(1972) 한방처방의 통계적 연구(I). 생약의 처방 출현빈도 및 기원분포. 생약학회지. 3: 57-64
3 서부일, 최호영(2004) 임상한방본초학, 787-792, 도서출판 영림사, 서울
4 鳥居塚 和生 緬著(2003) 生藥の藥效藥理, 59-73, 醫齒藥 出版株式會祉, 東京
5 Akao, T., Akao, T. and Kobashi, K. (1988) Glycyrrhzin stimulates growth of Eubacterium sp. strain GLH, a human intestinal anaerobe. Appl. Environ. Microbiol. 54: 2027-2030
6 Blois, M. S. (1958) Antioxidant determinations by the use of a stable free radical. Nature, 181: 1199-1200   DOI   ScienceOn
7 Yokozawa, T., Dong, E., Wu Liu, Z., Dura, H. and Nishioka, I. (1996) Antioxidation activity of Wen- Pi-Tang in vitro, Natural Medicines, 50: 243-246
8 Wroblewski, F. and J. S. LaDue (1955) Lactic dehydrogenase activity in blood, Proc. Soc. Exp. Biol. Med., 90: 210213
9 Kitagawa, I., Chen, W-Z., Taniyama, T., Harada, E., Hori, K., Kobayashi, M. and Ren, J. (1998) Quantitative determination of constituents in various Licorice roots by means of High Performance Liquid Chromatography. Yakugaku Zasshi. 118: 519-528
10 Hayes (1982) Principles and Method of Toxicology, Gabriel L. Plaa and William R. Hewitt Raben Press. 407-445
11 Kind, P. R. N and King, E. J. (1954) Estimation plasma phos- phatase by determination of hydrolysed phenol with aminoantipyrine, J Clin. Path., 7: 322-326   DOI   PUBMED
12 김남재(1997) 생약재의 가공 및 품질관리. 제 20회 한약 개발 심포지움 Proceedings. 생약재의 규격화 방안과 품질 평가법의 확립. 3-28. 한국생약학회
13 동의학연구소 편저 (1994) 동약법제. 61-64, 여강출판사, 서울
14 김호철(2001) 한약재 수치법제의 규격화 연구 보고서, 보건복지부
15 한국약학대학협의회 약전분과회 편저(2003) 대한약전 제8개정 해설서; 1056-1057, 도서출판 신일상사, 서울
16 國家藥典委員會(2005) 中華人民共和國藥典(2005年紋,1部), 59-60, 他學工業出版祉, 北京
17 Reitman, S. and Frankel, S. (1957) A colorimetric method for the determination of serum glutamic oxaloacetic acid and glutamic pyruvic transaminase, Am. J. Clin. Phathol., 28: 5663
18 北川 動, 三川 潮, 庄司順三, 友田正司, 西岡五夫(1992) 生樂學(第 4版). 233-235, 廣川書店, 東京
19 高木敬次郞, 木村正康, 原田正敏, 大塚恭男(1982) 和漢薬物學, 72-74, 南山堂, 東京
20 김남재, 진영호, 홍남두 (1995) 한약수치에 관한 연구(제 4보)-수치에 의한 감초중 Glycyrrhizin의 물리화학적 변화-. 생약학회지 26: 31-39
21 S. Maeda, K. Sudo, Y. Miyamoto, S. Takeda, M. Shinbo, M. Aburada, Y. Ikeya, H. Taguchi and M. Harada (1982) Pharmacological studies on Schzandra Fruits. II. Yakugaku Zasshi, 102: 579-588
22 전국한의과대학 본초학교수 공편저(1991 본초학, 540-541), 도서추판 영림사, 서울
23 Ching, H., Hsiu, S-L., Hou, Y-V., Chen, C-C. and Chao P-D.L. (2001) Comparison of pharmacokinetics between glycyrrhzin and glycyrrhetic acid in rabbits. J. Food. Drug Anal. 9: 67-71
24 생약학연구회(1992) 현대생약학, 274-276, 학창사, 서울
25 안덕균, 김호철 (2000) 한약포제학, 147-151, 일종사, 서울
26 김남재, 홍남두 (1996) 한약수치에 관한 연구(제 5보)-수치에 의한 감초의 성분변화 및 생리활성-. 생약학회지 27: 196-206