Browse > Article
http://dx.doi.org/10.15204/jkobgy.2018.31.4.001

Anti-osteoporotic Activity of Mixed Herbal Extract Involving Platycodon Grandiflorum Root in Osteoblastic MC3T3-E1 and Osteoclastic RAW 264.7 Cells  

Jung, Jae-In (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University)
Lee, Hyun-Sook (Dept. of Food Science & Nutrition, Dong-Seo University)
Kim, Hyung-Joon (Korean Medicine College, Se-Myung University)
Kim, Yong-Min (Dept. of Cosmetic Sciences, Se-Myung University)
Kim, Soo-Hyun (Research Institute, Guwoon Food)
Yoo, Dong-Jin (Research Institute, Guwoon Food)
Kim, Eun-Ji (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.31, no.4, 2018 , pp. 1-15 More about this Journal
Abstract
Objectives: Osteoporosis is considered a serious human disease. We developed an extract of mixed herbs containing root of Platycodon grandiflorum (ExMH-PGR), which is expected to be effective in preventing or treating osteoporosis. The aim of this study was to investigate the anti-osteoporotic effect of ExMH-PGR in osteoblastic MC3T3-E1 cells and osteoclastic RAW 264.7 cells. Methods: To examine the anti-osteoporotic effect of ExMH-PGR, osteoblast and osteoclast differentiation were induced and cultured with various concentrations of ExMH-PGR. Alkaline phosphatase (ALP) activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells were analyzed. Tartrate-resistant acid phosphatase (TRAP) activity and the formation of actin ring in RAW 264.7 cells were analyzed. Results: ExMH-PGR at concentration up to $25{\mu}g/mL$ significantly increased ALP activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells. ExMH-PGR at 50 to $200{\mu}g/mL$ significantly inhibited TRAP activity and the formation of actin ring in RAW 264.7 cells. Conclusions: These results demonstrate that ExMH-PGR stimulates osteoblastic activities and inhibits osteoclastic activities in in vitro systems, suggesting that ExMH-PGR might be considered as an anti-osteoporotic candidate for treatment of osteoporosis disease.
Keywords
Osteoporosis; Osteoblast; Osteoclast; Medicinal Herb; Platycodon Grandiflorum;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Jeong HM, et al. Saponins from the roots of Platycodon grandiflorum stimulate osteoblast differentiation via p38 MAPK- and ERK-dependent RUNX2 activation. Food Chem Toxicol. 2010; 48(12):3362-8.   DOI
2 Choi JH, et al. Platycodin D inhibits osteoclastogenesis by repressing the NFATc1 and MAPK signaling pathway. J Cellular Biochem. 2017;118(4):860-8.   DOI
3 Kim EJ, et al. Trans-10,cis-12-conjugated linoleic acid inhibits Caco-2 colon cancer cell growth. Am J Physiol Gastrointest Liver Physiol. 2001;283(2):357-67.   DOI
4 Puchtler H, Meloan SN, Terry MS. On the history and mechanism of Alizarin red S stains for calcium. J Histochem Cytochem. 1969;17(2):110-24.   DOI
5 Soya NS, Alles N. Osteoclast function and bone-resorbing activity: An overview. Biochem Biophys Res Commun. 2016; 476(3):115-20.   DOI
6 Scalbert A, et al. Dietary polyphenols and the prevention of diseases. Crit Rev Food Sci Nutr. 2005;45(4):287-306.   DOI
7 Hwang YP, et al. Saponins, especially platycodin D, from platycodon grandiflorum modulate hepatic lipogenesis in high-fat diet-fed rats and high glucose-exposed HepG2 cells. Toxicol Appl Pharmacol. 2013;267(2):174-83.   DOI
8 Ann JY, et al. A review of antiosteoporosis effects of herbal extracts in Korean journals. The Korean Journal of Oriental Medical Prescription. 2008;16(2):47-69.
9 Kim M. Effect of Platycodon Grandiflorum A. extract in bone metabolism in ovariectomized rats. Korean J. Oriental Physiology & Pathology. 2008;22(1):183-8.
10 Moon NY, Kim DC, Baek SH. Effect of Hyulbuchukeotang on inhibition of proliferation of uterine leiomyoma cells and cell apoptosis. The Journal of Oriental Obstetrics and Gynecology. 2006;19(2):186-98.
11 Tang W, Eisenbrand G. Chinese drugs of plant origin. Heidelberg:Springer. 1992:113-25, 199-201, 539-43, 567-88, 703-10, 1011-5.
12 Gou X, He G, Guo X. Poria cocos (Schw.) Wolf 茯苓(Fuling, Indian Bread). Dietary Chinese Herbs. 2015;87:781-8.
13 Sharma U, Pal D, Prasad R. Alkaline phosphatase: an overview. Indian J Clin Biochem. 2014;29(3):269-78.   DOI
14 Daneault A. Hydrolyzed collagen contributes to osteoblast differentiation in vitro and subsequent bone health in vivo. Osteoarthritis and Cartilage. 2014:22:S131.
15 Lian JB, Stein GS. Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation. Crit Rev Oral Biol Med. 1992;3(3):269-305.   DOI
16 Lee NK, et al. Endocrine regulation of energy metabolism by the skeleton. Cell. 2007;130(3):456-69.   DOI
17 Lee HW, et al. Berberine promotes osteoblast differentiation by Runx2 activation with p38 MAPK. J Bone Miner Res. 2008;23(8):1227-37.   DOI
18 Satue M, et al. Quercitrin and taxifolin stimulate osteoblast differentiation in MC3T3-E1 cells and inhibit osteoclastogenesis in RAW 264.7 cells. Biochem Pharmacol. 2013;86(10):1476-86.   DOI
19 Zhou L, et al. Berberine sulfate attenuates osteoclast differentiation through RANKL induced NF-kappaB and NFAT pathways. Int J Mol Sci. 2015;16(11):27087-96.   DOI
20 Teitelbaum SL. Bone resorption by osteoclasts. Science. 2000;289(5484):1504-8.   DOI
21 Hayman AR. Tartrate-resistant acid phosphatase (TRAP) and the osteoclast/immune cell dichotomy. Autoimmunity. 2008;41(3):218-23.   DOI
22 Teitelbaum SL. The osteoclast and its unique cytoskeleton. Ann. N.Y. Acad. Sci. 2011;1240(1):14-7.   DOI
23 Lee SK, et al. Platycodin D blocks breast cancer-induced bone destruction by inhibiting osteoclastogenesis and the growth of breast cancer cells. Cell Physiol Biochem. 2015;36(5):1809-20.   DOI
24 Schwayer C, et al. Actin rings of power. Dev Cell. 2016;37(6):493-506.   DOI
25 O'Ryan FS, Lo JC. Bisphosphonaterelated osteonecrosis of the jaw in patients with oral bisphosphonate exposure: clinical course and outcomes. J Oral and Maxillofac Surg 2012;70(8):1844-53.   DOI
26 van den Bergh JP, van Geel TA, Geusens PP. Osteoporosis, frailty and fracture: implications for case finding and therapy. Nat Rev Rheumatol. 2012;8(3):163-72.   DOI
27 He JB, Chen MH, Lin DK. New insights into the tonifying kidney-yin herbs and formulas for the treatment of osteoporosis. Arch Osteoporosis. 2017; 12(1):14.   DOI
28 Rossouw JE, et al. Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results from the Women's health initiative randomized controlled trial. JAMA 2002;288(3):321-33.   DOI
29 Zhang L, et al. Platycodon grandiflorus - an ethnopharmacological, phytochemical and pharmacological review. J Ethnopharmacol. 2015;164:147-61.   DOI
30 Ryu CS, et al. Evaluation of the total oxidant scavenging capacity of saponins isolated from Platycodon grandiflorum. Food Chem. 2012;132(1):333-7.   DOI
31 Wu J, et al. Cholesterol metabolism regulation and antioxidant effect of platycodin D on hyperlipidemic emulsionreduced rats. Afr J Pharm Pharmacol. 2011;5(22):2444-53.
32 Ashok BT, Ali R. The aging paradox: free radical theory of aging. Exp Gerontol. 1999;34(3):293-303.   DOI
33 Halliwell B. Oxidative stress and neurodegeneration: where are we now? J Neurochem. 2006;97(6):1634-58.   DOI
34 Zhao HL, et al. Hypocholesterolemic and anti-obesity effects of saponins from Platycodon grandiflorum in hamsters fed atherogenic diets. J Food Sci. 2008; 73(8):H195-200.   DOI
35 Choi YH, et al. Antiproliferative effects of saponins from the roots of Platycodon grandiflorum on cultured human tumor cells. J Nat Prod. 2010;73(11):1863-7.   DOI
36 Zhang Z, et al. Platycodin D, a triterpenoid saponin from Platycodon grandiflorum, suppresses the growth and ivasion of human oral squamous cell carcinoma cells via the NF-kB pathway. J Biochem Mol Toxicol. 2017;31(9) e21934:1-7.   DOI
37 Halliwell B. Oxidative stress and cancer: have we moved forward? Biochem J. 2007;401(1):1-11.   DOI
38 Evans P, Halliwell B. Micronutrients: oxidant/antioxidant status. Br J Nutr. 2001;85(S2):S67-S74.   DOI
39 Zhang L, et al. Anti-fungal and antibacterial activities of ethanol extracts of selected traditional Chinese medicinal herbs. Asian Pac J Trop Med. 2013; 6(9):673-81.   DOI
40 Ahn KS, et al. Platycodin D-induced apoptosis through nuclear factor-${\kappa}B$ activation in immortalized keratinocytes. Eur J Pharmacol. 2006;537(1-3):1-11.   DOI