• Korean Chemical Engineering Research
• /
• v.48 no.1
• /
• pp.88-92
• /
• 2010

DNA structure studies attract many interests in pharmaceutical, biochemical and medical disciplines. Among them, base pairs play a vital role in biological information transfer. Therefore, they need to be analyzed in various ways and the pair of guaninine and cytosine is the present analytical object. Separation of guanine and cytosine was researched by Aspen chromatography simulator and HPLC(High Performance Liquid Chromatography) experiments. Aspen chromatography simulation resulted in various chromatograms with changes of sample concentration, eluent flow rate and number of plate. The resolutions and yields of guanine and cytosine were calculated to obtain a best separation condition. $C_{18}$ HPLC column and water/methanol/acetic acid mixture(90/10/0.2) were used for separation of guanine and cytosine. HPLC parameters(resolution and number of theoretical plate) were calculated under different flow rates and sample concentrations. Aspen chromatography simulation and HPLC experimental results were compared with fair agreement.

• Korean Chemical Engineering Research
• /
• v.47 no.5
• /
• pp.615-619
• /
• 2009

(+)-$Tr{\ddot{o}}ger$'s base in $Tr{\ddot{o}}ger$'s base racemates that inhibits thromboxane A2($T{\times}A2$) synthase has been used to treat arteriosclerosis. Separation of (+)-$Tr{\ddot{o}}ger$'s base by chromatography has become a major concern. However separation experiments of (+)-$Tr{\ddot{o}}ger$'s base need time and consumables so that simulation with Aspen Chromatography could save time and costs by predicting the efficiency of separation. Injection amount and eluent flow rate were varied to compare the resolutions and yields of TB(-) and TB(+). Highest resolution and yield were attained at the eluent rate of 0.25 mL/min. Isotherms representing the relationship between mobile phase concentration and stationary phase concentration were changed to get the best separation with Ideal Adsorbed Solution(IAS) Statistical Lanmuir isotherms.

• KSBB Journal
• /
• v.23 no.2
• /
• pp.135-141
• /
• 2008

SMB (simulated moving bed) chromatography is a very useful utility for the separation of binary system. We simulated the separation of L-arabinose and L-ribose from the mixture by using lab-scale 4(1-1-1-1)-zone SMB chromatography. Preliminary experiments of PIM (pulse input method) were performed to measure adsorption isotherms of L-ribose and L-arabinose in $NH_2$ HPLC column, and experimental and simulated results from ASPEN chromatography were compared. To find the most suitable separation condition in SMB, we carried out a simulation in $m_2-m_3$ plane base on the triangle theory and calculated operating parameters (flow rate of four zone, switching time and feed concentration and so on) using ASPEN chromatography under the conditions of linear isotherms obtained from PIM.

• KSBB Journal
• /
• v.21 no.3
• /
• pp.220-223
• /
• 2006

Lysozyme is an important antibacterial material, as effective food preservative. A number of lysozymes are found in nature such as egg white, where exists about 3.5% of egg proteins. In this study, carboxymethyl cation exchange chromatography has been used for separation of lysozyme. A simulation study by ASPEN was also performed for saving time and cost in chromatography purification experiments. Important parameters in experimental chromatography were sample loading amount, NaCl concentration, and pH of eluent. Simulation results were successfully fitted with chromatograms from experiments with change of parameters mentioned above.

• Korean Chemical Engineering Research
• /
• v.49 no.6
• /
• pp.798-803
• /
• 2011

IgY(Immunoglobulin Yolk) is a specific antibody in egg yolk, and it protects human body from virus and antigen. There are a lot of egg yolk components such as lipoprotein and protein. To separate IgY, HPLC(High Performance Liquid Chromatography) and precipitation were used in a batch mode and SMB(Simulated Moving Bed) was adopted for continuous purification of yolk proteins. IgY and other proteins in yolk were separated by using three-zone SMB chromatography. Before performing SMB experiments, batch chromatography and PIM(pulse input method) were performed to find operation parameters and adsorption isotherms. The results of batch chromatography were compared with simulated results using Aspen chromatography. To find the most suitable separation condition in SMB chromatography, simulations in $m_2$-$m_3$ plane on the triangle theory were carried out. $m_2$ = 0.18, $m_3$ = 1.0 and ${\Delta}$t = 419 s are the best conditions for the highest purity of IgY. With this operating parameters(flow rate in three zone and switching time), the purity of raffinate results in 98.39% from Aspen chromatography simulation. Most of the simulation reached steadystate within second recycle.

• 한국생물공학회:학술대회논문집
• /
• 2005.10a
• /
• pp.558-558
• /
• 2005

• 한국생물공학회:학술대회논문집
• /
• 2005.04a
• /
• pp.373-373
• /
• 2005

• Korean Chemical Engineering Research
• /
• v.49 no.5
• /
• pp.623-627
• /
• 2011

Simulated moving bed(SMB) chromatography was simulated for separating loxoprofen racemates. Aspen chromatography simulator was utilized with Henry's constants of loxoprofen racemates which were obtained by batch chromatography experiments. Raffinate stream concentrations as well as purities were calculated with various $m_2$ and $m_3$ values in the triangle diagram obtained from two Henry's constants 7.9 and 10.1. Purity values are high under the conditions that the $m_2-m^3$ coordinates are near the left central region in the diagram and feed flow rates are lower. Concentration profiles of raffinate and extract streams along SMB columns explain the purity change in the case of increasing the column numbers installed at SMB.

• Korean Chemical Engineering Research
• /
• v.50 no.5
• /
• pp.866-873
• /
• 2012

IgY (Immunoglobulin Yolk) is a specific antibody in egg yolk, and it protects human body from virus and antigen. There are a lot of egg yolk components such as lipoprotein and protein. To separate IgY, HPLC (High Performance Liquid Chromatography) and precipitation were used in a batch mode and SMB (Simulated Moving Bed) was adopted for continuous purification of yolk proteins. IgY and other proteins in yolk were separated by using three-zone and four-zone SMB chromatography. Before performing SMB experiments, batch chromatography simulation parameters and adsorption isotherms were obtained. The parameters of batch chromatography were used to simulate SMB using Aspen chromatography. To compare three-zone and four-zone SMB chromatography, simulations in $m_2-m_3$ plane on the triangle theory were carried out. In terms of concentration and purity of both IgY and other lipoproteins, 3-zone SMB process is considered as ideal at the vertex of triangle ($m_2$, $m_3$=0.1, 1.1). 4-zone SMB yields the highest IgY purity at the coordinate ($m_2$, $m_3$=0.06, 0.5), which is the pure raffinate region. In 3-zone SMB without recycle, other lipoproteins in extract are largely affected in purity by small shift from the vertex of triangle ($m_2$, $m_3$=0.1, 1.1).

• Korean Chemical Engineering Research
• /
• v.52 no.1
• /
• pp.98-105
• /
• 2014

Lysozyme amounts to 0.3% in egg white and functions as an agent of cell lysis and activator of tissue reconstruction. Ion exchange chromatography is the most useful method of separation among affinity chromatography, ion exchange chromatography, and ultra-filtration. The aim of present study is to find the optimum pH and temperature for the separation of lysozyme in egg white within cation exchange gel filled glass column. And we compared results of experiments with those of simulations. Phosphate buffer was used, and pH and temperature were varied as 5~7 and $25{\sim}40^{\circ}C$ respectively. RP-HPLC was the tool for the retention time identification and quantitative analysis of lysozyme. OriginPro 8 measured the peak area of lysozyme chromatogram and quantified the eluted lysozyme. Largest amount of lysozyme was separated under the conditions of pH 5 and T $25^{\circ}C$.