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Experimental and Simulation Study of Lysozyme Separation in Cation Exchange Chromatography  

Kim, Jung-Ae (Department of Chemical Engineering, Chungnam National University)
Seong, Yeon-Kyeong (Department of Chemical Engineering, Chungnam National University)
Kim, In-Ho (Department of Chemical Engineering, Chungnam National University)
Publication Information
KSBB Journal / v.21, no.3, 2006 , pp. 220-223 More about this Journal
Abstract
Lysozyme is an important antibacterial material, as effective food preservative. A number of lysozymes are found in nature such as egg white, where exists about 3.5% of egg proteins. In this study, carboxymethyl cation exchange chromatography has been used for separation of lysozyme. A simulation study by ASPEN was also performed for saving time and cost in chromatography purification experiments. Important parameters in experimental chromatography were sample loading amount, NaCl concentration, and pH of eluent. Simulation results were successfully fitted with chromatograms from experiments with change of parameters mentioned above.
Keywords
Chromatography; lysozyme; ASPEN simulation;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
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3 Aspen Chromatography User Manual (2002)
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