• Journal of Plant Biotechnology
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• v.31 no.2
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• pp.103-108
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• 2004

This study was carried out to develop the efficient methods for the subsequent progeny of intersectional hybrid between Oriental and Asiatic lily hybrids. The pollen fertility and germination ability of 3 different allotetraploids (OAOA) after somatic chromosome doubling was ranged from 0 to 80 percent on artificial pollen germination medium. The number of BC$_1$ progeny using allotetraploid of F$_1$ OA-hybrid as male and female parent was different. The efficiency of BC$_1$ progeny production was increased when F$_1$OA-hybrids was used as male rather than as female parent. And in back crosses of F$_1$ OA-hybrids with the Asiatic and Oriental hybrids, Asiatic hybrids showed higher efficiency on BC$_1$ progeny production. The ploidy level between 2x or 4x Asiatic hybrid and allotetraploid F$_1$ OA-hybrid was determined and showed higher progeny production in 2x-4x crosses rather than 4x-4x.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.7 no.1
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• pp.145-151
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• 1969

To make clear the species of Korean Asiatic cotton, 21 Asiatic cotton varieties collected from China, Manchuria, Japan and Korea and preserved at suwon Crop Experiment Station were crossed to the tester stocks and cytological studies were made for their $F_1$ pollen mother cells. The results were summarized as follows: 1. In the all $F_1$ hybrids between the 21 collections and G. herbaceum tester stocks always one ring-four association was observed. 2. In the $F_1$ hybrids between additional 5 collections and G. arboreum tester stock ring-four or chain-four was not observed. 3. In the $F_1$ hybrids between G. herbaceum tester stocks and between G. arboreum tester stocks no ring-four or chain-four was observed, while in the $F_1$ hybrids between G. herbaceum tester stock and G. arboreum tester stock always one ring-four was observed. In the $F_1$ hybrids between collections also no ring-four or chain-four was observed. 4. From above results and together with the results reported in previous paper the species of Asiatic collections was inferred to the D.U. Gerstel's G. arboreum and their race was inferred to the J. Hutcinson's G. arboreum L. race sinense.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.221-225
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• 2009

Interspecific crossing was conducted to obtain hybrid lilly with better quality of adapting to the unfavorable environment using oriental Lilium spp. as a female parent. Average interspecific crossing rate between oriental and asiatic lines by cut-style pollination was as low as 29%. Although the average germination rate of zygotic hybrid embryo between oriental 'Rodolfa' ${\times}$ asiatic 'Toronto' was increased from 76.6% to 78.3%. on 114 or 1/6 strength MS medium compared to the control, the rate of zygotic embryos formation between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' was significantly enhanced up about 86~90% on 1/2 strength MS medium. Meanwhile, germination rate of interspecific hybrid embryo between oriental 'Snow Cristal' ${\times}$ asiatic 'Royal Trinity' showed up 100%. Germination rate of interspecific hybrids slightly increased by addition of $0.5-1.0mg{\cdot}L^{-1}$ GA. Germination rate of zygotic embryos between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' and oriental 'Belcanto' ${\times}$ L. callosum was highest on the MS medium containing $0.01mg{\cdot}L^{-1}$ NAA and $0.1mg{\cdot}L^{-1}$ kinetin, as 66.6% and 45.2% respectively.

• Korean Journal of Breeding Science
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• v.42 no.1
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• pp.100-108
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• 2010

This study was carried out to confirm the chromosome constitution and homoeologous recombination of progenies derived from various cross combination using tetraploid OA interspecific hybrid originated from mitotic chromosome doubling. Based on the chromosome analysis of progenies crossed reciprocally, there were only triploid progenies when crossed with diploid Asiatics as male or female parent. While only tetraploid progenies were produced when crossed tetraploid Asiatics or tetraploid OA hybrid with tetraploid OA hybrid, respectively. However, two types of progenies, that is, diploid and triploid plants, were produced from cross combinations between diploid Oriental hybrid and tetraploid OA hybrid. From the GISH analysis of OA hybrid, it was confirmed that diploid $F_1$ OA hybrid was consisted of 24 chromosomes (12 Oriental and 12 Asiatics) showing authentic OA hybrid. On the other hand, it was notified that triploid plants (3x=36) were consisted of 24 Asiatics lily chromosomes and 12 Oriental lily chromosomes by analysis of backcross progenies derived from either $A{\times}OA$ or $OA{\times}A$ crosses. In cross between tetraploid OA and OA, all the progenies were tetraploid with equal number of chromosomes without any homoeologous recombination, i.e. each 24 chromosomes of Oriental and Asiatics. In 2x-4x ($O{\times}OA$) cross combination, some progenies had 2x=24 chromosomes originated from only Oriental hybrid, and other progenies had 3x=36 chromosomes derived from 24 chromosomes of Oriental hybrid and 12 chromosomes of Asiatic hybrid. Only tetraploid Asiatics chromosomes without any Oriental one were produced in all the progenies from 4x-4x ($AA{\times}OA$) cross combination.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.353-357
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• 2003

We studies seed set in the interspecific F1 /backcross hybrids of Lilium species. In the interspecfic hybrid of L. longiflorum cv. Gelria with L.${\times}$ fomolongi cv. Raizan,93％ fruit set was obtained by stigmatic pollination in comparison to 53％ from cut-style pollination. Accordingly, the number of seed set resulting from stigmatic and cut-style pollination was 147 and 53, respectively. Pollination o( both stigmatic and cut-style pollination resulted in 47％ fruit set in the hybrid of L. longiflorum cv. Lorina with L.${\times}$ fomolongi cv. Raizan. However, stigmatic pollination formed 413 seeds, whereas only 24 seeds were obtained by cut-style pollination in this cross. The hybrid of L.${\times}$ fomolongi cv. Raizan with L. longiflorum cv. Come set 40％ fruit with a total of 43 seeds by stigmatic pollination. However, no fruit set was observed in cut-style pollination in this hybrid. Backcrossing the F1 hybrid by cut-style pollination of L. longiflorum cv. Lorina ${\times}$ Asiatic hybrid cv. Chicago with the latter parent led to 53％ fruit set, and 109 embryos were obtained. Likewise, backcrossing following cut-style pollination of L. longiflorum cv. Lorina ${\times}$ Asiatic hybrid cv. Corsia with the latter parent formed 67％ fruits and 107 embryos. However, in the remaining interspecific hybrids, cut-style pollination set no fruit.

• Journal of Practical Agriculture & Fisheries Research
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• v.24 no.2
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• pp.23-28
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• 2022

There is a need to breed and distribute of domestic varieties for Lilium Asiatic hybrids because most of the lily bulbs cultivated in Korea bred from foreign country. The collected Lilium Asiatic hybrids 'Butter Pixie' for a maternal line, 'Mona' for a paternal line based on the genetic resources from 2000 had been crossed self-fertilization from 2000 to 2006. In 2008, We developed Lilium Asiatic 'Yellow LiASong' as a F₁ hybrid which was crossed 'L₂-18-1' and 'L₂-21-4' those obtained by 4th self-fertilization. The flower of 'Yellow LiASong' is a bowl shaped single flower with no spots that 5.8 flowers per plant, which is a relatively many number compared other cultivars, and 15.5cm flower diameter with a medium size. And the flower inflorescence has umbellate and racemose while the attitude of longitudinal axis is erect. The plant height is 36.2cm with 35.5 leaves per plant and 10.7cm of the leaf length. Thus, The 'Yellow LiASong' showed a high consumer preference. Which has a longer flowering period due to having a relatively larger diameter and number of flowers than that of 'Tiny Bee', which is a comparable variety.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.2
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• pp.5-11
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• 2018

There is a need to breed and distribute domestic varieties for Lilium Asiatic hybrids because most of the lily bulbs cultivated in Korea imported from foreign country. The genetic resources of Lilium Asiatic hybrids, 'Buff Pixie', 'Orange Matrix' and 'Tiny Puppet', which were collected from 2001, had been self-pollinated from 2004 to 2012. In 2012, Lilium Asiatic 'Lovely LiASong' as an F₁ hybrid was bred through crossing 'L₂-12-1' and 'L₂-35-4' obtained by 4th self-fertilization. 'Lovely LiASong' had a bowl shaped single flower with a few spots and medium-sized flower, 8.7cm in flower diameter. The number of flowers per plant was 4.9, which was relatively more flowers than other cultivars. And the flower inflorescence had umbellate and racemose while the attitude of longitudinal axis was erect. The plant height was 33.4cm with 30.4 leaves per plant and 7.2cm in leaf length. The 'Lovely LiASong' showed a high consumer preference because it had a relatively longer flowering period, larger flower diameter and number of flowers than those of the control, 'Tiny Ghost'.

• FLOWER RESEARCH JOURNAL
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• v.16 no.1
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• pp.76-84
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• 2008

This study was conducted to find out the cause of discoloration after pressing yellow-colored flowers. Plant materials used were Lilium Asiatic Hybrids, Oncidium spp., Rosa hybrida, $Tulipa{\times}gesneriana$, Antirrhinum majus, Dianthus caryophyllus, Freesia hybrida, Zantedeschia elliottiana, Gerbera jamesonii, Helianthus annuus, and Ranunculus asiaticus. Thickness, ratio of length to width, and water content of petals did not considerably affect discoloration of pressed flowers. Expression of yellow color in petals was caused by carotenoids in Lilium Asiatic Hybrids, Oncidium spp., Rosa hybrida and $Tulipa{\times}gesneriana$, by flavonoids in Antirrhinum majus, Dianthus caryophyllus, Freesia hybrida and Zantedeschia elliottiana, and by coexistence of carotenoids and flavonoids in Gerbera jamesonii, Helianthus annuus and Ranunculus asiaticus. Carotenoids that expressed yellow color in petals affected significantly on discoloration in pressed flowers containing carotenoids alone or both carotenoids and flavonoids. However, discoloration was hardly ever found in yellow-colored species as affected by flavonoids. Dark treatment was effective for almost perfect preservation in color of pressed flowers, while light treatment resulted in severe discoloration.

• FLOWER RESEARCH JOURNAL
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• v.16 no.2
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• pp.118-123
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• 2008

For effective production of interspecific hybrids of Lilium and valuable new cultivars, we used Lilium Asiatic hybrid 'Tiny Ghost' as the female parent, and Oriental group, and Longiflorum group and Martagon as the male parents. Percentage pollen germination in Oriental hybrid 'Sorbonne' was the highest with 64%. As results of using normal style pollination and cut style pollination methods, all seed sets were formed in normal style pollinated treatment, except in 'Aktiva'. Pollen germination percent of Longiflorum hybrid 'Norina' was higher than that 'Gelria'. However, all crossings using 'Norina' failed to set seeds. Corresponding fertilization ratio was different from genetic compatibility, even in the same group. From the crossing using 'Aktiva' as the male parent one bulb was obtained derived from one embryo sac. And using 'Sorbonne' as the male parent, one plantlet was obtained after four ovule cultures. Crossing using 'Gelria' as the male parent five embryos, 15 embryo sacs, and seven ovules per one ovary were obtained. Among them, 18 plantlets germinated, with the germination percent of 66.7%. Crossings using L. hansonii, Martagon group, as the male parent resulted in 40% germination from five ovules.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.279-284
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• 2009

In order to recover fertility from sterile interspecific OA-1 $F_1$ hybrid (Oriental hybrid 'Mero Star' ${\times}$ Asiatic hybrid 'Connecticut King'), various concentrations (0.1, 0.3 and 0.5%) of caffeine were injected directly into flower buds and then confirmed the viability of OA-1 $F_1$ hybrid at the flowering time. After the caffeine treatment, fertilized $F_1$ hybrids were crossed as female with Asiatic hybrid 'Lanzarote' as male. Five plantlets were obtained from seven embryos of 16 pollinated flowers at 0.3% treatment of caffeine while 0.5% treatment of caffeine obtained one plant let and 0.1 % treatment of caffeine plantlet did not produce at all. Thus 0.3% of caffeine treatment was considered as optimum concentration to produce subsequent progenies and the OA-l $F_1$ hybrid treated with caffeine produced 51% of putative 2n gametes. Pollen germination of OA-2 ('Romero Star' ${\times}$ 'Lady Rosa') and OA-3 ('Expression' ${\times}$ 'Lady Rosa') was not differ between temperature treatment alone and in combination with caffeine and temperature treatment. In the reciprocal crosses of OA-1 and Asiatic hybrid 'Lanzarote' or Oriental hybrid 'Sorbonne', A ('Lanzarote') ${\times}$ OA-1 or OA-1 ${\times}$ A crosses showed better results than O ('Sorbonne') ${\times}$ OA-1 or OA-1 ${\times}$ a crosses in plant obtaining. All progenies obtained from A ${\times}$ OA-1 or OA-1 ${\times}$ A crosses were confirmed as triploids by GISH analysis.