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G-, C-, and NOR-banding of Korean Native Pig Chromosomes (한국재래돼지의 G-, C-, 및 NOR-banding)

  • Sohn, S.H.;Kweon, O.S.;Baik, K.H.;Jung, W.;Cho, E.J.;Kang, M.Y.
    • Journal of Animal Science and Technology
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    • v.45 no.6
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    • pp.901-910
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    • 2003
  • Using the G-, C-, and NOR-banding techniques, a karyotyping for Korean Native Pig was performed. Blood samples were collected from 50 male Korean Native Pigs that had been bred at the National Livestock Research Institute and then blood cells were prepared from in vitro cultures followed by karyotyping; G-, C-, and NOR-banding patterns of metaphase chromosomes were analyzed. The karyotype of Korean Native Pig is 38, XX or XY which consists of 5 pairs of submetacentric chromosomes(Group I), 2 pairs of acrocentric chromosomes with short p-arm(Group II), 5 pairs of medium metacentric chromosomes(Group III), 6 pairs of acrocentric chromosomes(Group IV) and metacentric X and Y sex chromosomes. On GTG-banding, the Korean Native Pig exhibited a typical and identical banding pattern in each homologous chromosomes. Overall chromosomal morphology and positions of typical landmarks of the Korean Native Pig were virtually identical to those of Committee for the Standardized Karyotype of the Domestic Pig(CSKDP). However, numbers of G-bands of the Korean Native Pig chromosomes were more than those of CSKDP. In chromosomes 1, 3, 5, 6, 7, 8, 13, 14, 15, 16, 17, 18 and X, the Korean Native Pig exhibited more separated bands as compared with CSKDP. In C-banding patterns, although the quantity of heterochromatin was variable in each chromosome, most of the Korean Native Pig chromosomes had heterochromatic C-bands on centromeres. However, the heterochromatic C-band was constantly observed on the whole Y chromosome. In AgNOR staining, the NORs were located at centromeres on the chromosomes 8 and 10. The number of NORs per metaphase ranged from 2 to 4 giving a mean value of 2.13. The number of NORs were distributed on all chromosome pair 10 but not on chromosome 8. The sizes of NORs were also differed between homologous chromosomes 8. Numbers of NORs of Korean Native Pig were significantly higher than those of Yorkshire. The pattern of pig NORs was polymorphic in breeds, individuals and cells, especially on chromosome 8.

Torque and mechanical failure of orthodontic micro-implant influenced by implant design parameters (교정용 마이크로 임플란트의 디자인이 토오크와 파절강도에 미치는 영향)

  • Yu, Won-Jae;Kyung, Hee-Moon
    • The korean journal of orthodontics
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    • v.37 no.3 s.122
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    • pp.171-181
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    • 2007
  • Objective: The present study was aimed at an analytical formulation of the micro-implant related torque as a function of implant size, i.e. the diameter and length, screw size, and the bony resistance at the implant to bone interface. Methods: The resistance at the implant to cancellous bone interface $(S_{can})$ was assumed to be in the range of 1.0-2.5 MPa. Micro-implant model of Absoanchor (Dentos Inc. Daegu, Korea) was used in the course of the analysis. Results: The results showed that the torque was a strong function of diameter, length, and the screw height. As the diameter increased and as the screw size decreased, the torque index decreased. However the strength index was a different function of the implant and bone factors. The whole Absoanchor implant models were within the safe region when the resistance at the implant/cancellous bone $(=S_{can})$ was 1.0 or less. Conclusion: For bone with $S_{can}$ of 1.5 MPa, the cervical diameter should be greater than 1.5 mm if micro-implant models of 12 mm long are to be placed. For $S_{can}$ of 2.0 MPa, micro-implant models of larger cervical diameter than 1.5 mm were found to be safe only if the endosseous length was less than 8 mm.

The Polyneuropathy in Patients with Chronic Obstructive Pulmonary Disease (만성 폐쇄성 폐질환 환자들의 다발성 말초신경병변에 대한 연구)

  • Baek, Jong-Cheul;Myung, Jae-Il;Kang, Heon-Seok;Kim, Yeong-Rock;Youm, Houng-Roul;Ryeu, Hyung-Seun;Lee, Soong;Kim, Wan;Noh, Jean-Yee
    • Tuberculosis and Respiratory Diseases
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    • v.44 no.4
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    • pp.806-814
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    • 1997
  • The incidence, type and distribution of polyneuropathy in patients with chronic obstructive pulmonary disease (COPD) were assessed and also analyzed the causative factors. Forty-four patients, mean age 66.1 years (42 male, 2 female), have been investigated with arterial gas analysis, pulmonary function test, clinical and electrodiagnostic studies. None of them had conditions known to affect the peripheral nervous system such as metabolic disorders or drugs. In a selected group of 44 patients, electrophysiological findings of polyneuropathy were found in 22 patients(50%), clinical polyneuropathy were diagnosed in 13 patients(9 patients were diagnosed by electrophysiological studies, 4 patients were normal by electrophysiological studies). These findings indicate that subclinical polyneuropathy(13 patients, 30%) more commonly occurs than clinical polyneuropathy(9 patients, 20%) in associated with COPD. In the patients with polyneuropathy, the lesions were predominant axonal degeneration, the changes were more involved in leg than arm, more frequently affected sensory fibers. We could not find etiologic factor to cause polyneuropathy in COPD patients.

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Assessment of Effect of Pulmonary Rehabilitation on Skeletal Muscle Metabolism by $^{31}P$ Magnetic Resonance Spectroscopy (호흡재활치료 전후 $^{31}P$ 자기공명분석법을 이용한 골격근대사의 변화에 관한 연구)

  • Cho, Won-Kyung;Kim, Dong-Soon;Choe, Kang-Hyeon;Park, Young-Joo;Lim, Tae-Hwan;Shim, Tae-Sun;Lim, Chae-Man;Lee, Sang-Do;Koh, Youn-Suck;Kim, Woo-Sung;Kim, Won-Dong
    • Tuberculosis and Respiratory Diseases
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    • v.44 no.5
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    • pp.1040-1050
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    • 1997
  • Pulmonary rehabilitation has been known to improve dyspnea and exercise tolerance in patients with chronic lung disease, although it does not improve pulmonary function. The mechanism of this improvement is not clearly explained till now; however some authors suggested that the improvement in the skeletal muscle metabolism after the rehabilitation could be a possible mechanism. The metabolc changes in skeletal muscle in patients with COPD are characterized by impaired oxidative phosphorylation which causes early activation of anaerobic glycolysis and excess lactate production with exercise. In order to evaluate the change in the skeletal muscle metabolism as a possible cause of the improvement in the exercise tolerance after the rehabilitation, noninvasive $^{31}P$ magnetic resonance spectroscopy(MRS) of the forearm flexor muscle was performed before and after the exercise training in nine patients with chronic lung disease who have undertaken intensive pulmonary rehabilitation for 6 weeks. 31p MRS was studied during the sustained isometric contraction of the dominant forearm flexor muscles up to the exhaustion state and the recovery period. Maximal voluntary contraction(MVC) force of the muscle was measured before the isometric exercise, and then 30% of MVC force was constantly loaded to each patient during the isometric exercise. After the exercise training, exercise endurance of upper and lower extremities and 6 minute walking distance were significantly increased(p<0.05). There were no differences of baseline intracellular pH (pHi) and inorganic phosphate/phosphocreatine(Pi/PCr). After rehabilitation pHi at the exercise and the exhaustion state showed a significant increase($6.91{\pm}0.1$ to $6.99{\pm}0.1$ and $6.76{\pm}0.2$ to $6.84{\pm}0.2$ respectively, p<0.05). Pi/PCr at the exercise and the recovery rate of pHi and Pi/PCr did not show significant differences. These results suggest that the delayed intracellular acidosis of skeletal muscle may contribute to the improvement of exercise endurance after pulmonary rehabilitation.

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A Study on the Women's Bodysuit Sleeve Block Construction Using Stretch fabrics (Stretch 소재를 사용한 여성용 Bodysuit Sleeve 원형 설계에 관한 연구)

  • Park Gin-Ah
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.12 s.148
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    • pp.1535-1545
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    • 2005
  • The study aimed firstly, to develop the women's bodysuit sleeve block construction method adopting the appropriate pattern reduction rates according to the fabric stretch property. Secondly, the details applied to the bodysuit sleeve block drafting (Dr the educational and industrial usage were proposed. For these, several distinguishing bodysuit sleeve pattern making methods(i.e. Joseph-Armstrong: T1, Shoben & Ward: T2, Esmod: T3 and Mixed Joseph-Armstrong: T4) were analyzed and divided into two categories that adopt 1) the equally (i.e., T1) and 2) the differently(i.e., T2, T3 & T4) distributed front and back armhole length measurements. Women's sleeve samples were made for the research using the same stretch fabric($50\%\;and\; 70\%$ in wale and course each) to the previous research. A group comprising 5 relevant experts evaluated the fit and comfort features of the samples. Experiments analyzed the appearance of sleeve samples focused on total 13 evaluation parts(including the front/side/back fit tolerance, sleeve centre line, sleeve length, appropriateness of the sleeve appearance balanced with the bodysuit and etc.): and performed the comfort test evaluating three kinds(vertical-front/vertical-side/ horizontal) of arm movements. The most appropriate bodysuit sleeve to fulfil the original aims of the study was suggested. The findings and suggestions throughout the study were: 1) the measurements and required reduction rates for the bodysuit sleeve block developed: outer sleeve length (with 1.0 RR), crown height(with 0.7 RR), front and back armhole lengths measured on the bodysuit blocks ($0\%$ ease amount), elbow width(0.9/0.95 RR), wrist girth measurements(from $12\%\;to\;18\%$ tolerances can be given to): and 2) the differently distributed front and back armhole length measurements resulted in the better fit and comfort through the research.

Rock-magnetic Properties of Chimneys from TA25 Seamount in the Tofua Arc, Southwest Pacific (통가 EEZ내 TA25 해저산에서 채취한 열수광체의 암석자기학적 특성 연구)

  • Kim, Wonnyon;Pak, Sang Joon;Lee, Kyeong Yong;Moon, Jai-Woon;Kim, Hyun Sub;Choi, Sun Ki
    • Economic and Environmental Geology
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    • v.46 no.3
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    • pp.207-214
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    • 2013
  • To identify rock-magnetic properties of volcanogenic hydrothermal sulfide deposits, chimneys were obtained from the Tofua Arc in Southwest Pacific, using a remotely operated vehicle (ROV) and Grab with AV cameras (GTVs). Three different types of chimneys used in this study are a high-temperature chimney with venting fluid-temperature of about $200^{\circ}C$ (ROV01), a low-temperature chimney of about $80^{\circ}C$ (GTV01), and an inactive chimney (ROV02). Magnetic properties of ROV01 are dominated by pyrrhotite, except for the outermost that experienced severe oxidation. Concentration and grain-size of ROV01 pyrrhotite are relatively low and fine. For GTV01, both magnetic concentration and grain-size increase from interior to margin. Pyrrhotite, dominant in the core, becomes mixed with hematite in the rim of the chimney due to secondary oxidation. High concentration and large grain-size of magnetic minerals characterize the ROV02. Dominant magnetic phases are pyrrhotite, hematite and goethite. In particular, the outermost rim shows a presence of magnetite produced by magnetotactic bacterial activity. Such distinctive contrast in magnetic concentration, grain-size and mineralogy among three different types of chimney enables the rock-magnetic study to characterize an evolution of hydrothermal deposits.

A Study of Genetic Polymonhisms of HLA-class I and II Genes Using Polymerase Chain Reaction (중합효소연쇄반응을 이용한 HLA-class I, II 유전자군의 유전적 다형성에 관한 연구)

  • Kyung-Ok Lee
    • Biomedical Science Letters
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    • v.4 no.1
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    • pp.11-25
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    • 1998
  • The HLA genes located in the short arm of chromosome 6 specify heterodimeric glycoproteins involved in the regulation of the immune response. Recently, in the elucidation of HLA polymorphism, serological and cellular typing methods have been replaced by DNA typing using polymerase chain reaction (PCR). The purpose of this study was to establish the HLA DNA typing methods and determine gene frequencies of HLA molecules in Koreans. PCR-SSP (sequence specific primers) and PCR-RFLP (restriction fragment length polymorphism) techniques were used for the analysis of HLA-A, -B, -C, DRBl genes and HLA-DQAl, DQBl, DPBl genes, respectively. The results of B-lymphoblastoid cells used for control experiment were consistent with the previous data identified in the 11th International Histocompatibility Workshop. Seventeen, 23, 16, 8, 16, 13 and 37 types of HLA-A, B, C, DQAl, DQBl, DPBl and DRBl alleles were found, respectively, in a total of unrelated 120 Korean individuals. The most frequent HLA alleles were $A^*$02 (27.0%), B$^*$40 (17.6%), Cw$^*$01 (19.2%), DQAl$^*$0301 (32.1%), DQBl$^*$0303 (12.9%), DPBl$^*$0501 (31.3%) and DRBl$^*$1501 (9.2%) among Koreans. This study shows that DNA typing method using PCR technique is a relatively simple, fast and practical tool for the determination of the HLA-class I and II genes. Moreover, the data of HLA gene frequencies could be useful for the Korean database before clinical applications, including organ and unrelated bone marrow transplantation, anthropological study, disease association and individual identification.

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Actual Status of and Measure for False Alarm of Electronic Security in Korea (한국 기계경비업무의 오경보 대응책)

  • Park, Dong-Kyun;Kim, Tae-Min
    • Korean Security Journal
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    • no.30
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    • pp.33-60
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    • 2012
  • False alarm of Electronic security causes various serious side effects such as decrease of electronic security guard's morale caused by unnecessary mobilization, increase of fatigue caused by workload increase, increase of electronic security company owner's management burden and decrease of electronic security service utilization rate caused by customer's distrust. Therefore, the study considered the Korean regulation related with false alarm of electronic security and proposed actual status of false alarm and measure for it. The study proposed systematic resolution assignments and political assignments in relation with the measure for false alarm. Systematic resolution assignments are as follows. First, electronic security company should construct electronic security system accurately from the initial step of security consulting and security planning related with target facility. Second, it is necessary to encourage installation and operation of video monitoring system. Third, sensor wiring should be separated. Fourth, the measures for false alarm depending on main system causes should be prepared. It is necessary to encourage the installation of 'arming disarming alarm sound' generator. In addition, the measures for false arm depending on the characteristics of sensor should be prepared and standardized. Fifth, system maintenance should be reinforced. Political assignments related with the measures for false alarm are as follows. First, it is necessary to reinforce education & training. Individual nurturing & education process should be run by electronic security company or the education focusing on the measure for false alarm should be performed in job training defined in "Security Industry Act". Second, it is necessary to establish and reinforce legal regulation and establish device. If police authority standardizes the documents related with false alarm, provides their forms and requires them for periodical reports or documents, it is expected that good measures for false alarm will be prepared on the basis of actual data in the future. Third, cooperation organization to discuss the measures for false alarm like 'Conference for False Alarm of Electronic Security' should be organized and operated. Fourth, interest and role of electronic security company and electronic security supervisor should be enlarged.

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Analysis of the Kinematic Characteristics at Entrance to the Straight Course from the Curvilinear Course in the 200m-Track Game (육상 200m 경기의 곡선주로에서 직선주로 진입 시 운동학적 특성분석)

  • Oh, Sei-Jin
    • Korean Journal of Applied Biomechanics
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    • v.12 no.2
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    • pp.51-63
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    • 2002
  • This study sought to identify the kinematic characteristics at entrance to the straight course from the curvilinear course in the 200m-track game. For this purpose, this study was conducted for 4 sprinters by setting the 10m-section combined from the curvilenear track to the straight course and shooting them with the camcorder. It was set up to include all the sections of analysis by using the framework of the control point knowing the coordinate of the space and actual analysis was conducted on the motion showing the best records by conducting it for each subject five times. As a result, the following conclusion was drawn: It was found that the subjects showed the average stride of 4.5${\pm}$0.41 times at the 10-meter section and the required time of 1.42${\pm}$0.04sec. They showed the ratio average stride to height of 1.25${\pm}$0.20% and the average speed of 7.06${\pm}$0.19m/s. The displacement in the center of gravity of the human body at the section combined from the curvilinear course to the straight course was moving along the inward course of the curvilinear course, and the displacement of the leg located at the outward direction(right) was found to be larger than that of the leg located at the inward direction(left). In the speed of the left and right hand segments, it was found that the speed of the right hand located in the outward direction was faster than that of the left hand located at the inward, and it was found that the subjects progressed in the curvilinear course. The subjects showed the larger angle of the shoulder joint when the upper arm was located in the forward direction than when the it was located in the backward direction. In the curvilinear course, they showed the lower value of the lateral angle of the trunk when the right foot located at the outward direction left the ground than when the left foot located at the inward direction left the ground. And it was found that the lateral angle of the trunk became lower with approaching the straight course.

The Kinematics Analysis of the Badminton High Clear Motion in Woman Middle School Student (여자중학생 배드민턴 하이클리어 동작의 운동학적 분석)

  • Kim, Chang-Bum;Ryu, Jae-Kwang
    • Korean Journal of Applied Biomechanics
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    • v.12 no.2
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    • pp.91-107
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    • 2002
  • This research got the following conclusion as result that analyzed high clear action kinematically to 4 C girls' junior high school badminton players who are situated in Chungchong-bukdo. 1. Most of the subject didn't rotate their right shoulder and elbow joint at back swing and moved speedy to shuttle cock. And an cooperation action of joint decreases displaying only a good action on both subject`s specification joint part. 2. When the subject S1 and S2 swing slowly and largely the joint of shoulder and elbow and then the speed of right wrist and racket head is fast, the cooperation action of joint is better than other subject. 3. An angle change of right shoulder showed angle that all subjects are great being caused in softness of joint and angular velocity was exposed that load enough Impact force and increase the speed of racket head as angular velocity decreases rapidly in Impact except subject S3. 4. All subjects of right elbow angle change showed similar form and was exposed that subject S2 sees form of impact stage serious bends from back swing and do not use force effectively in angular velocity. 5. Angle of right wrist appeared that the speed of shuttle cock is decelerated because fast bends of wrist is not formed shortly after Impact because all subject do not accomplish big angle shortly after back swing. Angular velocity can assume that the subject S1 and S4 are using and move fast strong snap shot offering angular velocity value of Impact stage sound (-). 6. While size of Impact stage knee angle accomplishes angle that is big both subject, hip joint angles sees small angle and is playing swing that do on upper body and arm without using strong waist force that is composition action with knee and hip joint.