• 한국수산과학회지
• /
• 제49권5호
• /
• pp.582-588
• /
• 2016

Seventy-nine Vibrio parahaemolyticus isolates from surface seawater from Gomso Bay, west coast of Korea, were analyzed for the presence of virulence genes and their susceptibility to 30 different antimicrobials. All 79 isolates were examined for the presence of two virulence genes (tdh or trh) using polymerase chain reaction (PCR); however, no isolates possessed either the tdh or trh gene. According to a disk diffusion susceptibility test, all of the strains studied were resistant to oxacillin, penicillin, and vancomycin, followed by ticarcillin (97.5%), ampicillin (96.2%), clindamycin (86.1%), erythromycin (10.1%), streptomycin (7.6%), cefoxitin (6.3%), amikacin (2.5%), and cephalothin (2.5%). However, all of the strains were susceptible to 19 other antimicrobials including cefepime, cefotaxime, chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/trimethoprim, and trimethoprim. All 79 isolates (100%) were resistant to four or more classes of antimicrobials, and two strains exhibited resistance to eight antimicrobial agents. The average minimum inhibitory concentrations (MICs) for V. parahaemolyticus for ampicillin, penicillin, ticarcillin, and vacomycin were 946.5, 1,305.9, 1,032.3, and 45.0 µg/mL, respectively.

• 대한의생명과학회지
• /
• 제26권4호
• /
• pp.302-309
• /
• 2020

Human Coxsackievirus B5 (HuCoxV-B5) infection has been associated with various diseases such as myocarditis, aseptic meningitis, hand-foot-and mouth-disease, and insulin-dependent diabetes. HuCoxV-B5 is a virus transmitted through the fecal-oral route and is detected in clinics, aquatic environments, food, shellfish, etc. and is one of the more important viruses in public health because of its incidence rate reported worldwide. In this study, a combination of SYBR Green-based real-time PCR primers for molecular diagnosis including monitoring of HuCoxV-B5 was selected and the optimal reaction conditions were established. Compared with the previously reported TaqMan probe-based real-time PCR method, assessments including a sample applicability test were performed. Results showed that the real-time PCR method developed in this study was suitable for a molecular diagnostic technique for detecting HuCoxV-B5. This study is expected to contribute to efforts in responding to safety accidents in public health because the proposed method facilitates rapid diagnosis of clinical patients. It can also be used as a specific monitoring tool of HuCoxV-B5 in non-clinical areas such as aquatic environments among others.

• 디지털융복합연구
• /
• 제18권10호
• /
• pp.181-194
• /
• 2020

유통시장의 변화에 따라 농·수·축산물의 보관과 유통 그리고 가공식품 및 신선식품의 보관과 운송에 대한 이슈가 급부상하고 있으며, 이에 따라 콜드체인이 물류 서비스의 한축으로 자연스럽게 주목을 받고 있다. 본 연구는 최근 들어 활기를 띠고 있는 냉동냉장 식품 등 콜드체인 유통과 관련하여 관심을 끌고 있는 수도권 지역 콜드체인 클러스터 구축에 대한 입지 경쟁력 평가를 목적으로 한다. 이를 위해 본 연구는 전문가의 지식을 효율적으로 추출하여 계량화할 수 있는 CFPR(Consistent Fuzzy Preference Relations) 분석기법을 활용하여 수도권 콜드체인 클러스터 후보지에 대한 경쟁력을 평가하였다. 연구 결과, 입지 경쟁력은 인천신항 배후부지, 경기 남부권(용인), 경기 서부권(김포 물류단지), 평택오성물류단지 순으로 우수한 것으로 나타났다. 특히 본 연구는 냉동·냉장 물류창고 운영 전문가의 지식을 추출하여, 수도권 콜드체인 클러스터 후보지에 대한 세부적인 경쟁력 평가를 실시하고, 최적의 클러스터 후보지를 제시하였다는 점에서 기존 연구들과 차별성을 가진다. 향후 연구에서는 설문 대상을 신선식품 물류센터의 소유권을 확보하기 위한 구매 및 구축 결정권을 가진 기업경영자, 대기업군, 공공기업 군 등으로 구분하여 조사할 필요가 있다.

• 한국양식학회:학술대회논문집
• /
• 한국양식학회 2002년도 춘계 한국양식학회 학술대회 발표요지
• /
• pp.171-172
• /
• 2002

Genomic DNA from the muscle of marsh clam (Corbicula leana)from Gochang was extrected in order to identify genetic differences and similarity by randomly amplified polymorphic DNAs-polymerase chain reaction. 3.28 of the 23.0 polymorphic bands per lane were found to be polymorphic in marsh clam. Also, about 4.34% of total polymorphic bands were either specific to marsh clam. The major common bands of 0.28 kb generated by primer OPB-15 (GGAGGGTGTT) were present in every individuals, respectively, which were polymorphic. This common bands which present in every individuals should be diagnostic of specific strains, species and-or their relatedness. Primer OPB-19 (ACCCCCGAAG) produced the highest number of specific bands, which was 12. The specific minor band of 0.07 kb was present in lane 22, which were polymorphic. Especially, only a specific band (1.35kg) identifying individuals was observed in lane 22.

• KSBB Journal
• /
• 제27권2호
• /
• pp.91-96
• /
• 2012

In this study, a newly synthesized thiazolidinedione derivative, TD49 with a highly selective algicide to red tide, was examined in order to evaluate the bioconcentration on aquatic organisms of coast. BAF (accumulation of TD49 by aquatic food chain) and BCF (accumulation of TD49 by sea water) were examined employing the shrimp (Fenneropenaeus chinensis) as the feed organism, and the olive flounder Paralichthys olivaceus as a consumer in marine ecosystem. Bioconcentration degree in sea water showed that the order in P. olivaceus was viscera > gill > muscle. The average BCF values of TD49 were 67.70, 63.32 and 20.25 at viscera, gill and muscle, respectively. Bioaccumulation degree using feed showed that the order in the organs of P. olivaceus was viscera > gill > muscle. The average BAF values of TD49 were 175.89, 114.88 and 32.59 at viscera, gill and muscle, respectively. When compared with two results, the accumulation by the food and water was higher than that by water. After the elimination experiment in sea water, the TD49 concentration was 2.81 nmole/g in the viscera and were not found in the gill and the muscle. More than 50% of the accumulated TD49 were eliminated from viscera in 7 days and all the accumulated TD49 were eliminated from gill and muscle in 7 days. On the other hand, the octanol/water partition coefficient (log $K_{ow}$) was measured to be 3.66 and experimental BCF of this study was 67.7.

• 한국수산과학회지
• /
• 제52권6호
• /
• pp.587-595
• /
• 2019

Eighty-three Vibrio parahaemolyticus isolates from surface seawater in Gomso Bay on the west coast of Korea, and commercial fisheries from Gunsan fisheries center were analyzed for the presence of virulence genes and susceptibility to 30 different antimicrobials. All 83 isolates were examined for the presence of two virulence genes (tdh or trh) using polymerase chain reaction; however, neither gene was found in any of the isolates. A disk diffusion susceptibility test, showed that all of the strains studied were resistant to clindamycin, oxacillin, ticarcillin, and vancomycin, and also revealed varying levels of resistance to ampicillin (98.8%), penicillin G (95.2%), streptomycin (20.5%), cefoxitin (14.5%), amikacin (6.0%), cephalothin (4.8%), and erythromycin (3.6%). However, all of the strains were susceptible to 19 other antimicrobial agents, including cefepime, cefotaxime, chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/trimethoprim, and trimethoprim. All 83 isolates (100%) were resistant to five or more classes of antimicrobials, and two strains exhibited resistance to ten antimicrobial agents. The average minimum inhibitory concentrations against V. parahaemolyticus of clindamycin, oxacillin, ticarcillin, and vancomycin were 55.9, 98.3, 499.3, and 44.3 ㎍/mL, respectively. These results provide new insight into the necessity for seawater sanitation in Gomso Bay and commercial fisheries, and provide evidence to help reduce the risk of contamination by antimicrobial-resistant bacteria.

• Fisheries and Aquatic Sciences
• /
• 제13권1호
• /
• pp.79-83
• /
• 2010

Since 1998, a new viral disease with high mortality has been consistently recorded in Korea in cultured carp, Cyprinus carpio. In this study, we investigated an epizootic of the disease that caused high mortality rates in carp obtained from 11 farms in Korea between 1999 and 2007. Assessment of koi herpesvirus (KHV) levels in diseased carp was carried out to determine if this virus was the etiologic agent of disease in this instance. High mortality rates in carp were recorded mainly in the spring and autumn at water temperatures between $19^{\circ}C$ and $24^{\circ}C$. Diseased fish typically showed surface discoloration, with a thick opaque mucus covering the body and gills. Protozoan parasites and bacteria were recovered from 7/29 (24%) and 2/26 (8%) of fish, respectively. Evidence of viral infection was marked; cytopathic effects (CPEs), characterized by cell rounding and an extended cytoplasm in fathead minnow (FHM) cells, were detected in 40/41 fish (98%). A high mortality rate (80%) resulted when supernatants of cell cultures showing CPEs were applied to previously healthy fish. KHV was detected by polymerase chain reaction in 6/41 fish (15%), but was not detected in supernatants obtained from cell cultures showing CPEs. These results suggest that KHV may not be the etiologic agent of the high mortality occurring among cultured carp in Korea; therefore, some other-as yet unidentified-infective agent must be responsible.

• Environmental Analysis Health and Toxicology
• /
• 제28권
• /
• pp.16.1-16.8
• /
• 2013

Objectives Fish vitellogenin (VTG) is produced in the female liver during oogenesis through the estradiol cycle and produced in the male liver by endocrine disrupting chemicals (EDCs) such as alkylphenols. In this study, we propose that the VTG concentration in the pale chub could be detected using monoclonal antibodies and polyclonal antibodies against vitellin (Vn) in a VTG enzyme-linked immunosorbent assay (ELISA) system. Methods Monoclonal antibodies and polyclonal antibodies were produced using the Vn extracted from the matured ovum of the ovary. The VTG was extracted from the plasma of the male pale chub. The Vn and VTG were confirmed by measuring the molecular weight of their proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the specificity of the antibodies was checked through western blotting methods. The assay system was validated with respect to optimal assay concentrations, specificity, recovery, and intra- and inter-assay variations. Results The Vn consisted of two protein bands with apparent molecular weights of 64 and 37 kDa. The SDS-PAGE indicated protein weights of 146 and 77 kDa in the VTG. The assay range was 15.6 ng/mL to 2,000 ng/mL, and the value of the intra- and inter-assay variations were within 10.0% and 14.7%, respectively. The recovery rate was $99.5{\pm}5.5%$. Conclusions A sandwich ELISA was developed that could be used to qualify the VTG of pale chub in screening for EDCs. Pale chub is an ideal species for observing estrogen activity in the environment because of its extensive habitat and extensive food chain. The ELISA developed here would be more favorable than those for other species for determining the effect of long-term food chain accumulation of EDCs in aquatic environments.

• 한국습지학회지
• /
• 제9권1호
• /
• pp.21-29
• /
• 2007

국내에서는 독성이 강한 화학물질 중 535종을 유독물로 지정하고 있다. 이들 유독물 중 약 10%만이 수질관련 기준으로 관리하고 있다. 타르색소도 화학물질이지만, 수계에 미치는 영향에 대한 자료가 미비한 상태이다. 본 연구에서는 형광 박테리아(Vibrio fischeri)와 큰물벼룩(Daphnia magna)을 이용하여 타르색소에 대한 생물검정을 수행하고자 한다. 국내에서 식용으로 허가되어 있는 7종 중 5종에 대한 타르색소의 독성을 평가하여 향후 연구의 기초자료를 제공하고자 한다. 물벼룩을 이용한 결과 독성순위는 적색 제2호, 황색 제5호, 적색 제3호, 황색 제4호, 청색 제1호순으로 그리고 형광성 박테리아를 이용한 Micorotox 독성시험은 15min-EC50을 기준으로 황색 제5호, 적색 제3호, 적색 제2호, 황색 제5호, 청색 제1호 순으로 나타났다. 타르색소들은 종류에 따라 수서생태계에 미치는 영향이 다르며, 수계에 타르색소들의 독성치 이상이 유출되었을 경우 먹이사슬에 의한 생물농축현상(bioconcentration)으로 수계와 인간에게 영향을 줄 것으로 생각된다.

• 한국환경복원기술학회지
• /
• 제18권2호
• /
• pp.1-17
• /
• 2015

Ecological reservoir is a multifunctional space where provides the functions of retention, animal habitat and improvement of ecosystem health and landscape. The ecological reservoir of Anteo Eco Park located in Gwangmyeong-si has established to functions for water purification, maintenance of healthy aquatic ecosystem. Because the Anteo Eco Park is located in the site where nonpoint pollutant materials flow in, Anteo Eco Park has potential factors which aquatic ecosystem health deteriorates and damages the habitat of golden frog(Rana plancyi chosenica) which is restoration target species. Therefore, the purpose of this study is to suggest the plan to manage the variables which impede the right functions of aquatic ecosystem by understanding the causal loop diagram for the change of water quality environment and the interaction of predator-prey through system thinking. The results are as follows. First, the study showed that the individual number of golden frog which is an indicator species of Anteo Eco Park is threatened by snakeheaded fish, which is an upper predator. Therefore, balanced food chain should be hold to protect golden frog by capturing the snakeheaded fish which is individual number's density is high, and the monitoring management of the individual number for predator(snakeheaded fish)-prey(golden frog) should be performed. Second, the study represented that water pollution and carnification is caused by the sediment as the dead body of the large emergent vegetation in the winter cumulates as sediment. Ecological reservoir in Anteo Eco Park has been managed by eliminating the dead body of the large emergent vegetation, but the guideline for the proper density maintenance of vegetation community is additionally needed. Lastly, the study showed that aquatic ecosystem of Anteo Eco Park where is contaminated from the inflow of nonpoint pollutants affects the individual number's decline of golden frog and snakeheaded fish. Accordingly, the creation of a buffer area and a substitution wetland is needed in the periphery of the Anteo Eco Park to control the inflow of nonpoint pollutants including organic matters, nutrients and heavy metals. This study will be helpful that Anteo Eco Park improves the regional landscape and maintain healthy aquatic ecosystem space for the park visitors including local residents.