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http://dx.doi.org/10.5657/KFAS.2019.0587

Antimicrobial Resistance and Minimum Inhibitory Concentrations of Vibrio parahaemolyticus Strains Isolated from Seawater and Commercial Fisheries  

Cho, Eui-Dong (Department of Food Science and Biotechnology, Kunsan National University)
Kim, Hee-Dai (Department of Biotechnology and Biomedicine, Chungbuk Provincial College)
Park, Kwon-Sam (Department of Food Science and Biotechnology, Kunsan National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.52, no.6, 2019 , pp. 587-595 More about this Journal
Abstract
Eighty-three Vibrio parahaemolyticus isolates from surface seawater in Gomso Bay on the west coast of Korea, and commercial fisheries from Gunsan fisheries center were analyzed for the presence of virulence genes and susceptibility to 30 different antimicrobials. All 83 isolates were examined for the presence of two virulence genes (tdh or trh) using polymerase chain reaction; however, neither gene was found in any of the isolates. A disk diffusion susceptibility test, showed that all of the strains studied were resistant to clindamycin, oxacillin, ticarcillin, and vancomycin, and also revealed varying levels of resistance to ampicillin (98.8%), penicillin G (95.2%), streptomycin (20.5%), cefoxitin (14.5%), amikacin (6.0%), cephalothin (4.8%), and erythromycin (3.6%). However, all of the strains were susceptible to 19 other antimicrobial agents, including cefepime, cefotaxime, chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/trimethoprim, and trimethoprim. All 83 isolates (100%) were resistant to five or more classes of antimicrobials, and two strains exhibited resistance to ten antimicrobial agents. The average minimum inhibitory concentrations against V. parahaemolyticus of clindamycin, oxacillin, ticarcillin, and vancomycin were 55.9, 98.3, 499.3, and 44.3 ㎍/mL, respectively. These results provide new insight into the necessity for seawater sanitation in Gomso Bay and commercial fisheries, and provide evidence to help reduce the risk of contamination by antimicrobial-resistant bacteria.
Keywords
Antimicrobial resistance; Minimum inhibitory concentration; Vibrio parahaemolyticus; Virulence genes;
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1 Munita JM and Arias CA. 2016. Mechanisms of antibiotic resistance. Microbiol Spectr 4, 1-37. https://doi.org/10.1128/microbiolspec.VMBF-0016-2015.
2 NCCLS (National Committee for Clinical Laboratory Standards). 2002. Performance standards for antimicrobial susceptibility testing. Twelfth informational supplement M100-S12. Wayne, Pennsylvania, U.S.A., 19087-19098.
3 No AR, Okada K, Kogure K and Park KS. 2011. Rapid detection of Vibrio parahaemolyticus by PCR targeted to the histone-like nucleoid structure (H-NS) gene and its genetic characterization. Lett Appl Microbiol 53, 127-133. https://doi.org/10.1111/j.1472-765X.2011.03072.x.   DOI
4 Ottaviani D, Leoni F, Talevi G, Masini L, Santarelli S, Rocchegiani E, Susini F, Montagna C, Monno R, D'Annibale L, Manso E, Oliva M and Pazzani C. 2013. Extensive investigation of antimicrobial resistance in Vibrio parahaemolyticus from shellfish and clinical sources, Italy. Int J Antimicrob Agents 42, 191-193. https://doi.org/10.1016/j.ijantimicag.2013.05.003.   DOI
5 Park K, Mok JS, Ryu AR, Kwon JY, Ham IT and Shim KB. 2018. Occurrence and virulence of Vibrio parahaemolyticus isolated from seawater and bivalve shellfish of the Gyeongnam coast, Korea, in 2004-2016. Mar Pollut Bull 137, 382-387. https://doi.org/10.1016/j.marpolbul.2018.10.033.   DOI
6 Park KS, Iida T, Yamaichi Y, Oyagi T, Yamamoto K and Honda T. 2000. Genetic characterization of DNA region containing the trh and ure genes of Vibrio parahaemolyticus. Infect Immun 68, 5742-5748.   DOI
7 Park KS, Ono T, Rokuda M, Jang MH, Okada K, Iida T and Honda T. 2004. Functional characterization of two type III secretion systems of Vibrio parahaemolyticus. Infect Immun 72, 6659-6665.   DOI
8 Takeda Y. 1988. Thermostable direct hemolysin of Vibrio parahaemolyticus. Method Enzymol 165, 189-193.   DOI
9 Tanil GB, Radu S, Nishibuchi M, Rahim RA, Napis S, Maurice L and Gunsalam JW. 2005. Characterization of Vibrio parahaemolyticus isolated from coastal seawater in peninsular Malaysia. Southeast Asian J Trop Public Health 36, 940-945.
10 Wang R, Zhong Y, Gu X, Yuan J, Saeed AF and Wang S. 2015. The pathogenesis, detection, and prevention of Vibrio parahaemolyticus. Front Microbiol 6, 144. https://doi.org/10.3389/fmicb.2015.00144.   DOI
11 Xie T, Wu Q, Xu X, Zhang J and Guo W. 2015. Prevalence and population analysis of Vibrio parahaemolyticus in aquatic products from South China markets. FEMS Microbiol Lett 362, fnv 178. https://doi.org/10.1093/femsle/fnv178.   DOI
12 Zhang L and Orth K. 2013. Virulence determinants for Vibrio parahaemolyticus infection. Curr Opin Microbiol 16, 70-77. https://doi.org/10.1016/j.mib.2013.02.002.   DOI
13 MFDS (Ministry of Food and Drug Safety). 2019. Food poisoning statistics. Retrieved from http://www.foodsafetykorea.go.kr/portal/healthyfoodlife/foodPoisoningStat.do?menu_grp=MENU_NEW02&menu_no=2786 on Oct 2, 2019.
14 Pazhani GP, Bhowmik SK, Ghosh S, Guin S, Dutta S, Rajendran K, Saha DR, Nandy RK, Bhattacharya MK, Mukhopadhyay AK and Ramamurthy T. 2014. Trends in the epidemiology of pandemic and non-pandemic strains of Vibrio parahaemolyticus isolated from diarrheal patients in Kolkata, India. PLoS Negl Trop Dis 8, e2815. https://doi.org/10.1371/journal.pntd.0002815.   DOI
15 Rowe-Magnus DA and Mazel D. 2002. The role of integrons in antibiotic resistance gene capture. Int J Med Microbiol 292, 115-125.   DOI
16 Ryu SH, Hwang YO, Park SG and Lee YK. 2010. Antibiotic susceptibility of Vibrio parahaemolyticus isolated from commercial marine products. Korean J Food Sci Technol 42, 508-513.
17 Ryu AR, Park K, Kim SH, Ham IT, Kwon JY, Kim JH, Yu HS, Lee HJ and Mok JS. 2017. Antimicrobial resistance patterns of Escherichia coli and Vibrio parahaemolyticus isolated from shellfish from the west coast of Korea. Korean J Fish Aquat Sci 50, 662-668. https://doi.org/10.5657/KFAS.2017.0662.   DOI
18 Sakazaki R, Tamura K, Kato T, Obara Y and Yamai S. 1968. Studies on the enteropathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus. 3. Enteropathogenicity. Jpn J Med Sci Biol 21, 325-331.   DOI
19 Shirai H, Ito H, Hirayama T, Nakamoto Y, Nakabayashi N, Kumagai K, Takeda Y and Nishibuchi M. 1990. Molecular epidemiological evidence for association of thermostable direct hemolysin (TDH) and TDH-related hemolysin of Vibrio parahaemolyticus with gastroenteritis. Infect Immun 58, 3568-3573.   DOI
20 Silva IP, Carneiro CS, Saraiva MAF, Oliveira TAS, Sousa OV and Evangelista-Barreto NS. 2018. Antimicrobial resistance and potential virulence of Vibrio parahaemolyticus isolated from water and bivalve mollusks from Bahia, Brazil. Mar Pollut Bull 131, 757-762. https://doi.org/10.1016/j.marpolbul.2018.05.007. Epub 2018 May 11.   DOI
21 Honda T and Iida T. 1993. The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysins. Rev Med Microbiol 4, 106-113.   DOI
22 Jones JL, Ludeke CH, Bowers JC, Garrett N, Fischer M, Parsons MB, Bopp CA and DePaola A. 2012. Biochemical, serological, and virulence characterization of clinical and oyster Vibrio parahaemolyticus isolates. J Clin Microbiol 50, 2343-2352. https://doi.org/10.1128/JCM.00196-12.   DOI
23 Kang CH, Shin Y, Kim W, Kim Y, Song K, Oh EG, Kim S, Yu H and So JS. 2016. Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from oysters in Korea. Environ Sci Pollut Res Int 23, 918-926. https://doi.org/10.1007/s11356-015-5650-9.   DOI
24 Kang CH, Shin Y, Jang S, Yu H, Kim S, An S, Park K and So JS. 2017. Characterization of Vibrio parahaemolyticus isolated from oysters in Korea: Resistance to various antibiotics and prevalence of virulence genes. Mar Pollut Bull 118, 261-266. https://doi.org/10.1016/j.marpolbul.2017.02.070.   DOI
25 Kang CH, Shin YJ, Yu HS, Kim SK and So JS. 2018. Antibiotic and heavy-metal resistance of Vibrio parahaemolyticus isolated from oysters in Korea. Mar Pollut Bull 135, 69-74. https://doi.org/10.1016/j.marpolbul.2018.07.007.   DOI
26 Kim SK, An SR, Park BM, Oh EG, Song KC, Kim JW and Yu HS. 2016. Virulence factors and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from the oyster Crassostrea gigas. Korean J Fish Aquat Sci 49, 116-123. https://doi.org/10.5657/KFAS.2016.0116.   DOI
27 Kim TO, Eum IS, Jo SM, Kim HD and Park KS. 2014. Antimicrobial-resistance profiles and virulence genes of Vibrio parahaemolyticus isolated from seawater in the Wando area. Korean J Fish Aquat Sci 47, 220-226. https://doi.org/10.5657/KFAS.2014.0220.   DOI
28 Acar JF and Goldstein FW. 1991. Disk susceptibility test. In: Antibiotics in laboratory medicine, Lorian V, ed. Williams & Wilkins, Baltimore, U.S.A., 17-52.
29 Deepanjali A, Kumar HS, Karunasagar I and Karunasagar I. 2005. Seasonal variation in abundance of total and pathogenic Vibrio parahaemolyticus bacteria in oysters along the southwest coast of India. Appl Environ Microbiol 71, 3575-3580.   DOI
30 Ellingsen AB, Olsen JS, Granum PE, Rorvik LM and Gonzalez-Escalona N. 2013. Genetic characterization of trh positive Vibrio spp. isolated from Norway. Front Cell Infect Microbial 3, 1-10. https://doi.org/10.3389/fcimb.2013.00107.
31 Gutierrez West CK, Klein SL and Lovell CR. 2013. High frequency of virulence factor genes tdh, trh, and tlh in Vibrio parahaemolyticus strains isolated from a pristine estuary. Appl Environ Microbiol 79, 2247-2252. https://doi.org/10.1128/AEM.03792-12.   DOI
32 Han AR, Yoon YJ and Kim JW. 2012. Antibiotic resistance and plasmid profile of Vibrio parahaemolyticus strains isolated from Kyunggi-Incheon coastal area. Korean J Microbiol 48, 22-28.   DOI
33 Kim TO, Um IS, Kim HD and Park KS. 2016. Antimicrobial resistance and minimum inhibitory concentrations of Vibrio parahaemolyticus strains isolated from Gomso bay, Korea. Korean J Fish Aquat Sci 49, 582-588. https://doi.org/10.5657/KFAS.2016.0582.   DOI
34 Kim YB, Okuda J, Matsumoto C, Takahashi N, Hashimoto S and Nishibuchi M. 1999. Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. J Clin Microbiol 37, 1173-1177.   DOI
35 Kuhl SA, Pattee PA and Baldwin NJ. 1978. Chromosomal map location of the methicillin resistance determinant in Staphylococcus aureus. J Bacteriol 135, 460-465.   DOI
36 Lee H, Oh YH, Park SG and Choi SM. 2007. Antibiotic susceptibility and distribution of Vibrio parahaemolyticus isolated from the seafood. Kor J Env Hlth 33, 16-20.
37 Lee HW, Lim SK and Kim MN. 2009. Characteristics of ampicillin-resistant Vibrio spp. isolated from a west coastal area of Korea peninsula. J Kor Fish Soc 42, 20-25.
38 Lee KW and Park KS. 2010. Antibiotic-resistance profiles and the identification of the ampicillin-resistance gene of Vibrio parahaemolyticus isolated from seawater. Korean J Fish Aquat Sci 43, 637-641.   DOI
39 Lee NH, Song HJ, Park CS, Kim HD and Park KS. 2011. Genetic characterization of ${\beta}$-lactamase (VPA0477) in Vibrio parahaemolyticus. Korean J Fish Aquat Sci 44, 597-604. https://doi.org/10.5657/KFAS.2011.0597.   DOI
40 Letchumanan V, Chan KG and Lee LH. 2014. Vibrio parahaemolyticus: a review on the pathogenesis, prevalence, and advance molecular identification techniques. Front Microbiol 5, 705. https://doi.org/10.3389/fmicb.2014.00705.   DOI
41 Li L, Meng H, Gu D, Li Y and Jia M. 2019. Molecular mechanisms of Vibrio parahaemolyticus pathogenesis. Microbiol Res 222, 43-51. https://doi.org/10.1016/j.micres.2019.03.003.   DOI
42 Lopatek M, Wieczorek K and Osek J. 2015. Prevalence and Antimicrobial Resistance of Vibrio parahaemolyticus Isolated from Raw Shellfish in Poland. J Food Prot 78, 1029-1033. https://doi.org/10.4315/0362-028X.JFP-14-437.   DOI
43 Makino K, Oshima K, Kurokawa K, Yokoyama K, Uda T, Tagomori K, Iijima Y, Najima M, Nakano M, Yamashita A, Kubota Y, Kimura S, Yasunaga T, Honda T, Shinagawa H, Hattori M and Iida T. 2003. Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae. Lancet 361, 743-749.   DOI
44 Martinez JL, Fajardo A, Garmendia L, Hernandez A, Linares JF, Martinez-Solano L and Sanchez MB. 2009. A global view of antibiotic resistance. FEMS Microbiol Rev 33, 44-65. https://doi.org/10.1111/j.1574-6976.2008.00142.x.   DOI