• Environmental Analysis Health and Toxicology
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• 제21권2호
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• pp.103-113
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• 2006

This study examined effects of crude oil on the phase II drug-metabolizing enzymes UDP-glucuronosyl transferase (UDPGT) and glutathione S-transferase (GST) in mussel Mytilus edulis and rockfish Sebastes schlegeli, a representative bivalve and a culture fish, respectively. This work also intended indirectly to evaluate the post impact recovery from the massive oil tanker spillage accidents occurred during the summer of 1995 in the sea area off Yosu City, Chonnam. For these, enzyme activities of UDPGT and GST were examined in the fish and mussel following laboratory exposure to fresh crude oil, weathered oil, field-obtained oil residues, or in the field biota samples. Decreased GST activity was observed in rock fish following exposure to oil-soluble fraction (OSF) of fresh oil. A similar diminished GST activity was also observed after OSF of artificially weathered oil. OSF of field oil residues retrieved from the spillage area approximately 1 year later also exerted a slight inhibition of GST to rockfish. There was neither a change in UDPGT in rockfish, nor were there changes in mussel in both enzymes to any oil fractions. We could not observe any difference in the two enzymes either in rockfish or mussel sampled from the field during $1.5{\sim}2.0$ years post spillage, indicating that their enzyme systems might had been recovered by the sampling time. In conclusion, it seems that the inhibition of GST activity in rockfish is a biomarker response to crude oil exposure. The results, however, must be interpreted with care, as the inhibition nay reflect various factors such as oil concentration, duration and water temperature.

• 디지털융복합연구
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• 제19권10호
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• pp.349-355
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• 2021

본 연구는 유방암 환자에게 수중 운동과 지상운동을 적용하여 비교 분석하고 암환자의 재활에 수중 운동의 효과를 규명하고자 시행하였다. 연구대상자는 유방암 환자 40명을 모집하여 수중 운동을 적용한 실험군 20명, 지상 운동을 적용한 대조군 20명으로 설정하였고, 수중운동과 지상운동을 각각 주 5회 8주간 중재한 후 어깨기능과 폐기능 삶의 질을 비교 분석하였다. 그 결과 그룹 간 어깨 기능장애는 실험군에서 대조군에 비해 더 큰 변화를 보여 통계적으로 유의한 차이를 보였고(p<.001), 노력성 폐활량과 1초간 노력성 호기량도 실험군에서 대조군에 비해 더 큰 변화를 보여 통계적으로 유의한 차이를 보였다(p<.05)(p<.001). 삶의 질의 변화 비교에서도 실험군이 대조군에 비해 더 큰 변화를 보여 통계적으로 유의한 차이를 보였다(p<.001). 본 연구 결과가 유방암 환자의 신체적 건강과 심리적, 사회적 건강을 회복시키고 관리함에 있어 효율적인 방법을 제시할 것으로 생각되며, 향후 연구를 발전시켜 폐암, 대장암 등의 환자들을 대상으로 한 연구와 다양한 수중운동 방법들의 적용으로 연구를 발전시켜 국내 유방 암 재활 치료의 기초자료를 제시하고자 한다.

• 한국어병학회지
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• 제34권2호
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• pp.233-241
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• 2021

Polydeoxyribonucleotide (PDRN)은 특정 adenosine 수용체에 작용하여 상처 치유 과정을 촉진하는 것으로 알려져 있다. 지금까지 어류의 조직에서 얻어진 PDRN과는 다르게, 최근 해초류인 Porphyra로부터 추출된 PDRN이 개발되었다. 본 연구는 나일틸라피아(Oreochromis niloticus)에서 인공적으로 피부의 상처를 유발한 뒤 Porphyra에서 추출한 PDRN을 이용하여 상처 치유 효과를 평가하였다. 상처의 회복은 시험 시작 후 1주내에는 일부만 회복되었지만, 2주내에 거의 완전히 회복되었다. 상처를 유발하기 전 PDRN을 10 및 20 mg/kg의 용량으로 5일간 사료로 투여한 후 상처 치유 효과를 관찰하였다. 상처 치유 효과는 육안적, 면적 및 색도측정으로 평가하였다. 본 연구에서 관찰된 Porphyra 유래의 PDRN에 의한 상처 치유 효과는 다양한 원인에 의해 발생하는 어류의 상처 치유 목적으로의 활용 가능성을 보여준다.

• 한국수산과학회지
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• 제43권4호
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• pp.293-297
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• 2010

To prevent amnesic shellfish poisoning (ASP) resulting from the consumption of shellfish contaminated with domoic acid, the quantitative analysis of domoic acid is very important. We validated a high performance liquid chromatography (HPLC) method for accurate and precise quantification of domoic acid. A clear peak and the isolation of domoic acid resulted on injecting a 50% methanol extract of CRM-ASP-Mus-c mussel reference material using HPLC. The limit of detection of domoic acid under the established HPLC conditions was $0.10\;{\mu}g/g$, and the limit of quantification of the toxin under the same conditions was $0.25\;{\mu}g/g$. The intra-accuracy and precision for domoic acid in CRM-ASP-Mus-c were 90.7-95.7% and 0.28-22.25%, respectively. The inter-accuracy and precision for domoic acid were 89.1-97.1% and 1.7-4.1%, respectively. The mean recovery of domoic acid in methanol extracts from ten species of marine invertebrates was 88.6-1105.1%.

• Fisheries and Aquatic Sciences
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• 제7권3호
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• pp.99-108
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• 2004

Vitellogenin (VTG) was purified from serum of $estradiol-l7{\beta}-treated rockfish$(Sebastes schlegeli) by precipitation with $EDTA-Mg^{2+}$ and ammonium sulfate and two step chromatography (anion exchange chromatography and gel permeation chromatography) was performed on FPLC system. Rockfish VTG (rfVTG) was characterized and its properties were determined. The monomers have apparent, molecular mass of about 188 kDa as indicated by SDS-PAGE. Amino acid composition analysis of rfVTG was similar to VTG from other oviparous teleosts. Cysteine and lysine were present at relatively high level. Leucine was present at relatively lower level than in other species. The N-terminal amino acid sequence was evaluated to identify rfVTG. Western blot analysis using an antibody against the purified VTG showed that the antibody reacted with both plasma of $estradiol-l7{\beta}-treated rockfish$ treated male and purified VTG, whereas there was no reaction with male serum of the control. An ELISA was developed using monoclonal and polyclonal antibodies against rfVTG. The assay range was 3.2 ng/mL and 1,000 ng/mL and the value of the intra and inter assay variations were within $9.7{\%}$ and $11.2{\%}$, respectively. Recovery rate was $96.8{\%}$. The sandwich ELISA could be useful for the detection of VTG and could be good for screening of estrogenic compounds.

• 한국수산과학회지
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• 제41권3호
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• pp.176-181
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• 2008

To evaluate the effective use of endoprotease from squid viscera as a food processing aid, various methods of fractionating endoprotease from viscera of the Argentina shortfin squid (Illex argentinus) were evaluated. The endoprotease-positive fractions of each fractionation were fraction II (30-40%, w/w) with cold acetone, fraction IV (50-60% saturation) with ammonium sulfate, fraction UF with anion exchange chromatography, and fraction II (15-24 kDa) with gel filtration. The specific activities (approximately 25 U/mg) of the fractions using ammonium sulfate and gel filtration were higher than the others. Total azocaseinolytic activity and recovery of the positive fraction using gel filtration were 806.95U and 37.82%, respectively, and were the highest among the positive fractions. Based on the results, gel filtration was the most efficient method for fractionating endoprotease from the viscera of Illex argentinus.

• Fisheries and Aquatic Sciences
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• 제8권1호
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• pp.17-26
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• 2005

The ultrastructure of germ cells during spermatogenesis and the structural changes in the epithelial cells of the seminal vesicle with testicular development in male Neptunea (Barbitonia) arthritica cumingii were investigated monthly based on electron microscopic and histologic observations. N. arthritica cumingii (Gastropod: Buccinidae) undergoes internal fertilization and possesses a modified type of spermatozoon, which is approximately 20$\mu$m long. The axoneme of the tail flagellum consists of nine peripheral pairs of microtubules and one central pair. Many spermatozoa occur in the acini of the testis in the ripe stage and are transported to the seminal vesicles in the accumulating phase. In males, the monthly gonadosomatic index began to increase in September and reached a maximum in February. Subsequently, it decreased rapidly after April. The testis of this species can be classified into four developmental stages: the active (August to September), ripe (October to July), copulation (April to July), and recovery (July to August) stages. Structural changes in the epithelial cells of the seminal vesicles of this species could be classified into three phases: (1) S-I (resting), (2) S-II (accumulating), and (3) S-III (spent) phases. The morphology and structure of the epithelial cells of the seminal vesicle differed in each phase; the cells were cuboidal, squamous, or columnar in the resting, accumulating, or spent phases, respectively.

• Fisheries and Aquatic Sciences
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• 제13권2호
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• pp.190-194
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• 2010

In recent years, several southern coastal fish farms in Korea have experienced 2-30% mortality in striped beakperch suffering from bacterial infections during the spring. In this study, we identified a bacterium isolated from diseased striped beakperch as Pseudomonas anguilliseptica via a biochemical test and 16S rDNA sequence analysis. To evaluate the susceptibility of striped beakperch to P. anguilliseptica, $4.39\times10^7$ or $4.39\times10^5$ CFU/fish of bacteria were injected intraperitoneally at $18\pm1^{\circ}C$ into fish weighing 5.5 g. Cumulative mortalities reached 100% and 45% in the $4.39\times10^7$ and $4.39\times10^5$ CFU/fish infected groups, respectively. Experimentally infected fish showed cell-associated inflammation as well as bacteria in the kidneys and spleen. This study is the first report of striped beakperch mortality caused by P. anguilliseptica, which has pathogenicity in striped beakperch.

• 한국수산과학회지
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• 제33권6호
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• pp.554-558
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• 2000

참서대 암컷의 생식소중량지수는 6월에 가장 높았다. 난모세포의 발달양식은 난군동시발달형이며, 초기 성장기 난모세포에서 난병과 난황핵이 관찰되었다. 생식주기는 성장기 (2${\~}$5월), 성숙기 (5${\~}$6월), 완숙 및 산란기 ($6{\~}8$월) 그리고 회복 및 휴지기 ($8{\~}2$월)로 구분된다 전장 $TL 28.1{\~}30.8 cm$의 개체당 절대포란수는 2,197 개였으며, 체중 g당 상대포란수는 10.0개였다.

• Fisheries and Aquatic Sciences
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• 제6권3호
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• pp.149-154
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• 2003

The characteristics of pollutant loads from the various sources and seawater quality in Kwangyang Bay were evaluated. Total flow rate was estimated to be $10,868,066.8 m^3/day$ with a flow rate of R2l (the Seomjin River) as the highest one. Total COD, TN and TP loads of the input rivers and the ditches were about 27,591.8, 25,029.6 and 586.4 kg/day, respectively. Wastewater discharging loads was the greatest contributors to pollutant loads in the inner part of Kwangyang Bay. COD values in the inner part of the bay was over 3.0 mg/L, which exceeded the seawater quality criteria III of Korea. The average values of DIN and DIP were 8.62 ${\mu}gN/L\;and\;1.26\;{\mu}gP/L$, respectively. The limiting factor for algal growth was DIN. In he total discharging loads of the watershed from unit loading estimations, BOD, TN and TP were 9,132.3, 2,727.2 and 304.2 kg/day, respectively. In addition, municipal sewage by the population as pollution sources and the city of Kwangyang as administrative district had the highest loads. For a appropriate water quality recovery of Kwangyang Bay, it is suggested that it is essential to estimate reduction rate of total pollutant loads by water quality modeling.