Environmental Analysis Health and Toxicology

환경독성보건학회 (The Korean Society of Environmental Health and Toxicology)
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원유의 노출이 담치와 조피볼락의 phase II 약물대사효소 UDP-glucoronosyl transferase 및 glutathione S-transferase의 활성에 미치는 영향

Activity Changes in Phase II Drug-metabolizing Enzymes UDP-Glucoronosyl Transferase and Glutathione S-Ttansferase to Crude Oil Exposure in Mussel and Rockfish

  • 박관하 (군산대학교 해양과학대학 수산생명의학과 및 식품공학과) ;
  • 김주완 (군산대학교 해양과학대학 수산생명의학과 및 식품공학과) ;
  • 박음미 (군산대학교 해양과학대학 수산생명의학과 및 식품공학과) ;
  • 임철원 (군산대학교 해양과학대학 수산생명의학과 및 식품공학과) ;
  • 최민순 (군산대학교 해양과학대학 수산생명의학과 및 식품공학과) ;
  • 최선남 (군산대학교 해양과학대학 수산생명의학과 및 식품공학과) ;
  • 황인영 (인제대학교 자연과학대학 환경공학부) ;
  • 김정상 (경북대학교 농업생명과학대학 동물공학과)
  • Park Kwan-Ha (Departments of Aquatic Life Medicine and Food Science & Engineering, Kunsan National University) ;
  • Kim Ju-Wan (Departments of Aquatic Life Medicine and Food Science & Engineering, Kunsan National University) ;
  • Park Eum-Mi (Departments of Aquatic Life Medicine and Food Science & Engineering, Kunsan National University) ;
  • Lim Chul-Won (Departments of Aquatic Life Medicine and Food Science & Engineering, Kunsan National University) ;
  • Choi Min-Soon (Departments of Aquatic Life Medicine and Food Science & Engineering, Kunsan National University) ;
  • Choe Sun-Nam (Departments of Aquatic Life Medicine and Food Science & Engineering, Kunsan National University) ;
  • Hwang In-Young (School of Environmental Science & Engineering, Inje University) ;
  • Kim Jung-Sang (Department of Animal Science & Biotechnology, Kyungpook National University)
  • 발행 : 2006.06.01
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초록

This study examined effects of crude oil on the phase II drug-metabolizing enzymes UDP-glucuronosyl transferase (UDPGT) and glutathione S-transferase (GST) in mussel Mytilus edulis and rockfish Sebastes schlegeli, a representative bivalve and a culture fish, respectively. This work also intended indirectly to evaluate the post impact recovery from the massive oil tanker spillage accidents occurred during the summer of 1995 in the sea area off Yosu City, Chonnam. For these, enzyme activities of UDPGT and GST were examined in the fish and mussel following laboratory exposure to fresh crude oil, weathered oil, field-obtained oil residues, or in the field biota samples. Decreased GST activity was observed in rock fish following exposure to oil-soluble fraction (OSF) of fresh oil. A similar diminished GST activity was also observed after OSF of artificially weathered oil. OSF of field oil residues retrieved from the spillage area approximately 1 year later also exerted a slight inhibition of GST to rockfish. There was neither a change in UDPGT in rockfish, nor were there changes in mussel in both enzymes to any oil fractions. We could not observe any difference in the two enzymes either in rockfish or mussel sampled from the field during $1.5{\sim}2.0$ years post spillage, indicating that their enzyme systems might had been recovered by the sampling time. In conclusion, it seems that the inhibition of GST activity in rockfish is a biomarker response to crude oil exposure. The results, however, must be interpreted with care, as the inhibition nay reflect various factors such as oil concentration, duration and water temperature.

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