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http://dx.doi.org/10.5657/fas.2010.13.2.190

Recovery of Pseudomonas anguilliseptica from Diseased Striped Beakperch (Oplegnathus fasciatus) in Korea  

Kim, Seok-Ryel (Pathology Division of National Fisheries Research and Development Institute)
Park, Myoung-Ae (Pathology Division of National Fisheries Research and Development Institute)
Kitamura, Shin-Ichi (Center for Marine Environmental Studies, Ehime University)
Jung, Sung-Ju (Division of Food Science and Aqualife Medicine, Chonnam National University)
Oh, Myung-Joo (Division of Food Science and Aqualife Medicine, Chonnam National University)
Publication Information
Fisheries and Aquatic Sciences / v.13, no.2, 2010 , pp. 190-194 More about this Journal
Abstract
In recent years, several southern coastal fish farms in Korea have experienced 2-30% mortality in striped beakperch suffering from bacterial infections during the spring. In this study, we identified a bacterium isolated from diseased striped beakperch as Pseudomonas anguilliseptica via a biochemical test and 16S rDNA sequence analysis. To evaluate the susceptibility of striped beakperch to P. anguilliseptica, $4.39\times10^7$ or $4.39\times10^5$ CFU/fish of bacteria were injected intraperitoneally at $18\pm1^{\circ}C$ into fish weighing 5.5 g. Cumulative mortalities reached 100% and 45% in the $4.39\times10^7$ and $4.39\times10^5$ CFU/fish infected groups, respectively. Experimentally infected fish showed cell-associated inflammation as well as bacteria in the kidneys and spleen. This study is the first report of striped beakperch mortality caused by P. anguilliseptica, which has pathogenicity in striped beakperch.
Keywords
Pseudomonas anguilliseptica; Striped beakperch; 16S rDNA; Pathogenicity;
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1 Oh SY, Nor CH, Kang RS and Myoung JG. 2006. Effects of water temperature and photoperiod on the oxygen consumption rate of fasted juvenile parrot fish, Oplegnathus fasciatus. Ocean and Polar Res 28, 407-413.   과학기술학회마을   DOI   ScienceOn
2 Saitou N and Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406-425.
3 Tort L, Gomez E, Montero D and Sunyer JO. 1996. Serum hemolytic and agglutinating activity as indicators of fish immunocompetence: their suitability in stress and dietary studies. Aqua Int 4, 31-41.
4 Wakabayashi H and Egusa S. 1972. Characteristics of a Pseudomonas sp. from an epizootic of pond-cultured eels (Anguilla japonica). Bull Jap Soc Sci Fish 38, 577-587.   DOI
5 Weisburg WG, Bams SM, Pelletier DA and Lane DJ. 1991. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697-703.
6 Wiklund T and Bylund G. 1990. Pseudomonas anguilliseptica as a pathogen of salmonid fish in Finland. Dis Aquat Org 8, 13-19.   DOI
7 Haenen OL and Davidse MA. 2001. First isolation and pathogenicity studies with Pseudomonas anguilliseptica from diseased European eel Anguilla anguilla (L.) in The Netherlands. Aquaculture 196, 27-36.   DOI   ScienceOn
8 Jung SJ and Oh MJ. 2000. Iridovirus-like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Temminck et Schlegel), in southern coastal areas of the Korean peninsula. J Fish Dis 23, 223-226   DOI   ScienceOn
9 Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111-120.   DOI
10 Kusuda R, Dohata N, Fukuda Y and Kawai K. 1995. Pseudomonas anguilliseptica infection of striped jack. Fish Pathol 30, 121-122.   DOI
11 Oh MJ, Kitamura SI, Kim WS, Park MK, Jung SJ, Miyadai T and Ohtani M. 2006. Susceptibility of marine fish species to a megalocytivirus, turbot iridovirus, isolated from turbot, Psetta maximus (L.). J Fish Dis 29, 415-421.   DOI   ScienceOn
12 Muroga K, Jo Y and Yano M. 1973. Studies on red spot disease of pond-cultured eels-I. The occurrence of the disease in eel culture ponds in Tokushima prefecture in 1972. Fish Pathol 8, 1-9.   DOI
13 Nakai T, Kanemori Y, Nakajima K and Muroga K. 1985. The fate of Pseudomonas anguilliseptica in arti-ficially infected eels Anguilla japonica. Fish Pathol 19, 253-258.   DOI
14 Nakajima K, Muroga K and Hancock REW. 1983. Comparison of fatty acid, protein and serological properties distinguishing outer membranes of Pseudomonas anguilliseptica strains from those of fish pathogens and other Pseudomonads. Int J Systemat Bacteriol 33, 1-8.   DOI
15 Domenech A, Fernandez-Garayzabal JF, Lawson P, Garcia JA, Cutuli MT, Blanco M, Gibello A, Moreno MA, Collins MD and Dominguez L. 1997. Association of Pseudomonas anguilliseptica infection with 'winter disease' in sea bream, Sparus aurata L.. Aquaculture 156, 317-326.   DOI   ScienceOn
16 Berthe FCJ, Michel C and Bernardet JF. 1995. Identification of Pseudomonas anguilliseptica isolated from several fish species in France. Dis Aquat Org 21, 151-155.   DOI
17 Chao CB, Chen CY, Lai YY, Lin CS and Huang HT. 2004. Histological, ultrastructural, and in situ hybridization study on enlarged cells in grouper Epinephelus hybrids infected by grouper iridovirus in Taiwan (TGIV). Dis Aquat Org 58, 127-142.   DOI
18 Domenech A, Fernandez-Garayzabal JF, García JA, Cutuli MT, Blanco M, Gibello A, Moreno MA and Dominguez L. 1999. Association of Pseudomonas anguilliseptica infection with 'winter disease' in sea bream, Sparus aurata L.. J Fish Dis 22, 69-71.   DOI   ScienceOn
19 Ferguson HW, Collins RO, Moore M, Coles M and MacPhee DD. 2004. Pseudomonas anguilliseptica infection in farmed cod, Gadus morhua L.. J Fish Dis 27, 249-253.   DOI   ScienceOn