Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Recovery of Pseudomonas anguilliseptica from Diseased Striped Beakperch (Oplegnathus fasciatus) in Korea

  • Kim, Seok-Ryel (Pathology Division of National Fisheries Research and Development Institute) ;
  • Park, Myoung-Ae (Pathology Division of National Fisheries Research and Development Institute) ;
  • Kitamura, Shin-Ichi (Center for Marine Environmental Studies, Ehime University) ;
  • Jung, Sung-Ju (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Oh, Myung-Joo (Division of Food Science and Aqualife Medicine, Chonnam National University)
  • Received : 2009.12.29
  • Accepted : 2010.06.04
  • Published : 2010.06.30
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Abstract

In recent years, several southern coastal fish farms in Korea have experienced 2-30% mortality in striped beakperch suffering from bacterial infections during the spring. In this study, we identified a bacterium isolated from diseased striped beakperch as Pseudomonas anguilliseptica via a biochemical test and 16S rDNA sequence analysis. To evaluate the susceptibility of striped beakperch to P. anguilliseptica, $4.39\times10^7$ or $4.39\times10^5$ CFU/fish of bacteria were injected intraperitoneally at $18\pm1^{\circ}C$ into fish weighing 5.5 g. Cumulative mortalities reached 100% and 45% in the $4.39\times10^7$ and $4.39\times10^5$ CFU/fish infected groups, respectively. Experimentally infected fish showed cell-associated inflammation as well as bacteria in the kidneys and spleen. This study is the first report of striped beakperch mortality caused by P. anguilliseptica, which has pathogenicity in striped beakperch.

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References

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