• Title/Summary/Keyword: Applied Genetics and Breeding

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Metabolism Characteristics of Acifluorfen Tolerant in Somaclones of Eastern Black Nightshade (Solanum ptycanthum Dun.)

  • Yu, Chang-Yeon;Li, Cheng Hao;Lee, Dong-Wook;Lim, Jung-Dae;Kim, Jae-Kwang
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.2
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    • pp.126-130
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    • 2005
  • Acifluorfen tolerance charateristics determined the involvement of absorption, translocation, and metabolism in acifluorfen tolerance. Less than 6% of the applied $^{14}C-acifluorfen$ was absorbed. There were no differences in acifluorfen absorption between susceptible and tolerant somaclones. More $^{14}C-acifluorfen$ was translocated in the susceptible than the tolerant somaclones. The susceptible somaclone did not metabolize acifluorfen while some somaclones (i.e.,EBN-3A) metabolized $^{14}C-acifluorfen$. Nomenclature: Acifluorfen, 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid; eastern black night shade, Solanum ptycanthum Dun.,$#^3$ SOLPT.

Physico-chemical Properties of Giant Embryo Brown Rice (Keunnunbyeo)

  • Choi, In-Duck;Kim, Deog-Su;Son, Jong-Rok;Yang, Chang-Inn;Chun, Ji-Yeon;Kim, Kee-Jong
    • Journal of Applied Biological Chemistry
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    • v.49 no.3
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    • pp.95-100
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    • 2006
  • Brown rice with a giant embryo (GE) was observed on the quality parameters of the enlargement of embryo, nutritional components, and physical properties, in comparison to normal embryo brown rice (NE). Also, the effects of germination on the quality parameters were examined. GE embryo was approximately 2.68 times larger than of NE rice. Total free sugars were significantly higher in GE rice (71.96 vs. 41.17 mg/100 g), and germinated rice increased in fructose, but decreased in sucrose and maltose. No significant difference in mineral contents was found in GE and NE rice and their germinated rice, whereas a significant increment was observed on reducing sugars and gamma-amino butyric acid (GABA) contents in GE rice. The lower water absorption index (WAI) of GE rice resulted in relatively lower pasting viscosity, whereas the increased WSI in germinated rice might be attributable to the significant increment of soluble components in GE rice.

Virulence Differentiation of Eight Turnip mosaic virus Isolates Infecting Cruciferous Crops

  • Choi, Hong-Soo;Sohn, Seong-Han;Yoon, Moo-Kyoung;Cheon, Jeong-Uk;Kim, Jeong-Soo;Were, Hassan Karakacha;Cho, Jang-Kyung;Kim, Kook-Hyung;Takanami, Yoichi
    • The Plant Pathology Journal
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    • v.21 no.4
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    • pp.369-376
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    • 2005
  • Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Th 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pfl, r 9-10, Rhcql-2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r9-10, Stock and Rh-cql-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pfl isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.

Study on Waxy Corn IX. Amylogram Properties, Antioxidant Activity and Texture Analysis on the Developed Waxy Corn Hybrids (찰옥수수 연구 IX. 찰옥수수 교잡종의 아밀로그램 특성, 항산화성 및 식미관련 종실의 물성)

  • Bok, Tae-Gyu;Lee, Moon-Sub;Choi, Yun-Pyo;Cha, Hui-Jung;Baek, Seoung-U;Jo, Yang-Hee;Lee, Hee-Bong
    • Korean Journal of Breeding Science
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    • v.43 no.1
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    • pp.62-67
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    • 2011
  • This study was carried out to gain basic informations about amylogram, antioxidant activity function and physicochemical properties for kernel of the newly developed waxy corn hybrids. The used materials were produced in 2008 and cultivated at breeding farm of CNU in 2009. From amylogram analysis, peak and minimum viscosity of the used hybrids were appeared in CNU08H-71 and CNU08H-69 hybrid, respectively. DPPH free radical scavenging effect marked as election donating ability was highly appeared in CNU08H-15 and CNU08H-69 hybrid, while CNU08H-h102 hybrid was the lowest. Hardness of kernel was highly appeared in CNU08H-35 hybrid, and chewiness and gumminess were also the highest in this hybrid, but those of CNU08H-h105 hybrid were the lowest. In these facts, we confirmed that the used hybrids were very different among traits related to amylogram, functions and table qualities. Accordingly, development of the new waxy corn hybrid will be profitable to select and develop as a crossing combination including many excellent traits.

Genetic Study on the Resistance of Blast Disease in the Rice Variety Tongil (II) (수도 통일품종의 도열병저항성에 관한 유전학적 연구(II))

  • Kwon Shin Han;Oh Jeung Haing
    • Korean journal of applied entomology
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    • v.15 no.4 s.29
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    • pp.163-168
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    • 1976
  • The present study was conducted to determine the number of genes controlling resistance of variety Tongil to three prevalent blast strains and any relationships among the genes in the inheritance of resistance of the variety to blast. Tongil was crossed with five local varieties and the progenies were tested with blast isolates E-7 and 100-14 by injection inoculation method. Results indicated that a tingle dominant gene conditioned resistance of variety Tongil and resistance to the isolate E-7 was controlled by a gene different from that controlling resistance to the isolate 100-14. In previous study it was identified that the gene controlling resistance to the isolate E-7 was different from the gene for resistance to isolate T-1. Therefore based on the foregoing results it may be inferred that the variety Tongil has at least three or more resistant genes to blast.

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Isolation and Characterization of Dikaryotic Mutants from Pleurotus ostreatus by UV Irradiation

  • Joh, Joong-Ho;Kim, Beom-Gi;Kong, Won-Sik;Yoo, Young-Bok;Chu, Kyo-Sun;Kim, Nam-Kuk;Park, Hye-Ran;Cho, Bong-Gum;Lee, Chang-Soo
    • Mycobiology
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    • v.32 no.2
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    • pp.88-94
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    • 2004
  • Protoplasts of the wild type strain of Pleurotus osteatus were mutagenized with UV light, and 3,000 colonies were examined for abnormal mycelial and fruiting phenotypes. Forty one strains displayed variant phenotypes in mycelia and fruiting processes. The variant phenotypes were classified into 6 groups: (1) auxotrophic strains, which are incapable of growing on minimal media and can only grow when provided with their specific requirements; (2) abnormal vegetative strains, which grow very slowly on minimal and complete media; (3) primordiumless strains, which fail to develop to the formation of primordia; (4) maturationless strains, which form primordia, but do not form mature fruiting bodies; (5) specifically colored strains, which have Specific bluish grey or bluish white pileus; (6) poorly spored strains, which fail to produce basidiospore or which produce few spores. These variant strains may be useful in genetic breeding programs and for the studies of fungal development and genetics.

Long-Term Cold Storage and Plant Regeneration of Suspension Cultured Somatic Embryos of Eleutherococcus senticosus Maxim (가시오갈피 현탁배양 체세포배의 저온장기저장 및 식물체 재분화)

  • Li, Cheng Hao;Lim, Jung-Dae;Heo, Kwon;Kim, Myong-Jo;Lee, Chan-Ok;Lee, Jae-Geun;Cui, Xue Shu;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.6
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    • pp.494-499
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    • 2004
  • A method for long-term conservation of somatic embryos of Eleutherococcus senticosos was described. Suspension cultured globular somatic embryos were successfully conservated for 36 months at $4^{\circ}C$. The embryos resumed growth within two weeks when returned to MS liquid medium containing $0.2\;mg/{\ell}$. 2,4-dichlorophenoxy acetic acid. The optimal condition for cell proliferation was achieved when somatic embryos cultured at $32^{\circ}C$ in 1/3 MS liquid medium, and about 1.2 g of embryogenic cell was induced from 150 globular embryos after 6 weeks of suspension culture. The embryogenic cells produced from these somatic embryos exhibited normal plant regeneration on auxin-free medium.

Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

  • Ragil Angga, Prastiya;Tri Wahyu, Suprayogi;Aldea Erian, Debora;Ani, Wijayanti;Anny, Amalia;Deny, Sulistyowati;Aras Prasetiyo, Nugroho
    • Animal Bioscience
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    • v.36 no.2
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    • pp.209-217
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    • 2023
  • Objective: The conservation of Bali bulls, the Indonesian native breed of cattle, is crucial for cattle breeding in Indonesia. To guarantee the spread of Bali bulls through artificial insemination the quality of the frozen semen must be high. To this end, adding an extender material to semen that increases spermatozoa's survival during cryopreservation is important. Green tea extract (GTE) can be used as cryoprotectant because its high antioxidant activity can help avoid reactive oxygen species formation. Methods: Semen of five Bali bulls from the National Artificial Insemination Center at Singosari, Indonesia was collected routinely twice a week. First, fresh semen inspection was performed to determine the feasibility of using Bali bulls as animal samples. The extender used in this study was Tris-based egg yolk. The samples were divided into four treatments: T0, no GTE added to the extender; T1, 0.05 mg GTE plus 100 mL extender; T2, 0.10 mg GTE plus 100 mL extender; and T3, 0.15 mg GTE plus 100 mL extender. The semen freezing process was conducted according to standard procedures and sperm quality parameters, i.e., sperm motility, viability, abnormalities, and membrane integrity observed pre-freezing and post-thawing. Results: There were significant differences in total motility, progressive motility, viability, and integrity membrane of Bali bull sperm at both pre-freezing and post-thawing after adding GTE into the extender. In contrast, there were no differences in abnormalities among treatments. Conclusion: Adding GTE at a 0.15 mg into 100 mL Tris-based egg yolk extender can improve the quality of cryopreserved Bali bull sperm.

ADDITIVE AND HETEROSIS EFFECTS ON MILK YIELD AND BIRTH WEIGHT FROM CROSSBREEDING EXPERIMENTS BETWEEN HOLSTEIN AND THE LOCAL BREED IN BANGLADESH

  • Hirooka, H.;Bhuiyan, A.K.F.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.8 no.3
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    • pp.295-300
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    • 1995
  • Data from purebred and crossbred cattle involving Holstein and the Local breed in Bangladesh were used to estimate the genetic effects on average daily milk yield and birth weight A total of 877 records on average daily milk yield for 4 types of breed groups and a total of 418 records on birth weight for 5 breed groups were analyzed. Two different methods were applied in this study; the least squares analysis of variance approach and the linear regression approach. Breed group effects were highly significant for both average daily milk yield and birth weight. The result showed that straightbred Holstein produced the highest milk yield and the 7/8 crosses ranked highest in birth weight For the two traits, the additive breed effect was highly significant, whereas the individual heterosis effect was not significant. Furthermore, this study showed a negative maternal heterosis for average daily milk yields and a positive maternal heterosis for birth weight Comparing the breed least squares means obtained from the linear regression approach revealed that straightbred Holstein produced the highest average milk yield and the 3/4 crosses were predicted to have the largest birth weight. It is indicated that the linear regression approach can adequately separate the genetic component of performance, estimate unknown crossbreeding parameters and predict unknown performance of crosses which are not include in the original data.

Cryopreservation of Forest Tree Seeds: A Mini-Review

  • Gantait, Saikat;Kundu, Suprabuddha;Wani, Shabir Hussain;Das, Prakash Kanti
    • Journal of Forest and Environmental Science
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    • v.32 no.3
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    • pp.311-322
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    • 2016
  • Since forest trees form the basis of forest ecosystem, their prolong subsistence is crucial for various flora and fauna. The foremost challenges to sustain the forest ecosystem comprise of the declining forest tree population accompanied with structural changes due to afforestation and exploitation of forest area, environment changes, pests, pollution, and introgressive hybridization. For ex situ conservation approach, in vitro techniques encompass basic role for conserving tree genetic resources, predominantly where natural propagules like recalcitrant seed might not be appropriate for long-term conservation. The practice includes restricted growth techniques, conventional micropropagation, production and storage of synthetic seeds, and cryopreservation. Even though these practices have been applied chiefly to herbaceous species, but recently, woody species were also focused upon. Key conceptions, challenges and techniques for forest tree seed conservation are discussed briefly in this review with special emphasis on some successful cryopreservation approaches for long-term storage.