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Long-Term Cold Storage and Plant Regeneration of Suspension Cultured Somatic Embryos of Eleutherococcus senticosus Maxim  

Li, Cheng Hao (Forest Genetics and Tree Breeding Lab., College of Foresry, Mortheast Forestry Uni.)
Lim, Jung-Dae (Division of Applied Plant Sci., Kangwon Natl. Univ.)
Heo, Kwon (Division of Applied Plant Sci., Kangwon Natl. Univ.)
Kim, Myong-Jo (Division of Applied Plant Sci., Kangwon Natl. Univ.)
Lee, Chan-Ok (Division of Applied Plant Sci., Kangwon Natl. Univ.)
Lee, Jae-Geun (Division of Applied Plant Sci., Kangwon Natl. Univ.)
Cui, Xue Shu (Division of Applied Plant Sci., Kangwon Natl. Univ.)
Yu, Chang-Yeon (Division of Applied Plant Sci., Kangwon Natl. Univ.)
Publication Information
Korean Journal of Medicinal Crop Science / v.12, no.6, 2004 , pp. 494-499 More about this Journal
Abstract
A method for long-term conservation of somatic embryos of Eleutherococcus senticosos was described. Suspension cultured globular somatic embryos were successfully conservated for 36 months at $4^{\circ}C$. The embryos resumed growth within two weeks when returned to MS liquid medium containing $0.2\;mg/{\ell}$. 2,4-dichlorophenoxy acetic acid. The optimal condition for cell proliferation was achieved when somatic embryos cultured at $32^{\circ}C$ in 1/3 MS liquid medium, and about 1.2 g of embryogenic cell was induced from 150 globular embryos after 6 weeks of suspension culture. The embryogenic cells produced from these somatic embryos exhibited normal plant regeneration on auxin-free medium.
Keywords
Eleutherococcus senticosus; suspension culture; somatic embryo; 2,4-dichlorophenoxy acetic acid;
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