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http://dx.doi.org/10.7747/JFES.2016.32.3.311

Cryopreservation of Forest Tree Seeds: A Mini-Review  

Gantait, Saikat (AICRP on Groundnut, Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya)
Kundu, Suprabuddha (Department of Agricultural Biotechnology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya)
Wani, Shabir Hussain (Division of Genetics and Plant Breeding, Faculty of Agriculture, Wadura, Sher-E-Kashmir University of Agriculture Sciences and Technology of Kashmir)
Das, Prakash Kanti (Department of Agricultural Biotechnology, Faculty Centre for Integrated Rural Development and Management, School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda University, Ramakrishna Mission Ashrama)
Publication Information
Journal of Forest and Environmental Science / v.32, no.3, 2016 , pp. 311-322 More about this Journal
Abstract
Since forest trees form the basis of forest ecosystem, their prolong subsistence is crucial for various flora and fauna. The foremost challenges to sustain the forest ecosystem comprise of the declining forest tree population accompanied with structural changes due to afforestation and exploitation of forest area, environment changes, pests, pollution, and introgressive hybridization. For ex situ conservation approach, in vitro techniques encompass basic role for conserving tree genetic resources, predominantly where natural propagules like recalcitrant seed might not be appropriate for long-term conservation. The practice includes restricted growth techniques, conventional micropropagation, production and storage of synthetic seeds, and cryopreservation. Even though these practices have been applied chiefly to herbaceous species, but recently, woody species were also focused upon. Key conceptions, challenges and techniques for forest tree seed conservation are discussed briefly in this review with special emphasis on some successful cryopreservation approaches for long-term storage.
Keywords
conservation; desiccation; liquid nitrogen; orthodox seed; recalcitrant seed;
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