• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.598-607
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• 2016

This study was conducted to evaluate the amount of nitrogen (N) top dressing based on the normalized difference vegetation indices (NDVI) by ground based sensors for leaf perilla under the polyethylene house. Experimental design was the randomized complete block design for five N fertilization levels and conventional fertilization with 3 and 4 replications in Gumsan-gun and Milyang-si field, respectively. Dry weight (DW), concentration of N, and amount of N uptake by leaf perilla as well as NDVIs from sensors were measured monthly. Difference of growth characteristics among treatments in Gumsan field was wider than Milyang. SPAD-502 chlorophyll meter reading explained 43.4% of the variability in N content of leaves in Gumsan field at $150^{th}$ day after seedling (DAS) and 45.9% in Milyang at $239^{th}$ DAS. Indexes of red sensor (RNDVI) and amber sensor (ANDVI) at $172^{th}$ day after seedling (DAS) in Gumsan explained 50% and 57% of the variability in N content of leaves. RNDVI and ANDVI at $31^{th}$ DAS in Milyang explained 60% and 65% of the variability in DW of leaves. Based on the relationship between ANDVI and N application rate, ANDVI at $172^{th}$ DAS in Gumsan explained 57% of the variability in N application rate but non significant relationship in Milyang field. Average sufficiency index (SI) calculated from ratio of each measurement index per maximum index of ANDVI at $172^{th}$ DAS in Gumsan explained 73% of the variability in N application rate. Although the relationship between NDVIs and growth characteristics was various upon growing season, SI by NDVIs of ground based remote sensors at top dressing season was thought to be useful index for recommendation of N top dressing rate of leaf perilla.

• Journal of Intelligence and Information Systems
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• v.20 no.3
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• pp.1-18
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• 2014

Over the past years RFID/SN has been an elementary technology in a diversity of applications for the ubiquitous environments, especially for Internet of Things. However, one of obstacles for widespread deployment of RFID technology is the inherent unreliability of the RFID data streams by tag readers. In particular, the problem of false readings such as lost readings and mistaken readings needs to be treated by RFID middleware systems because false readings ultimately degrade the quality of application services due to the dirty data delivered by middleware systems. As a result, for the higher quality of services, an RFID middleware system is responsible for intelligently dealing with false readings for the delivery of clean data to the applications in accordance with the tag reading environment. One of popular techniques used to compensate false readings is a sliding window filter. In a sliding window scheme, it is evident that determining optimal window size intelligently is a nontrivial important task in RFID middleware systems in order to reduce false readings, especially in mobile environments. In this paper, for the purpose of reducing false readings by intelligent window adaption, we propose a new adaptive RFID data cleaning scheme based on window sliding for a single tag. Unlike previous works based on a binomial sampling model, we introduce the weight averaging. Our insight starts from the need to differentiate the past readings and the current readings, since the more recent readings may indicate the more accurate tag transitions. Owing to weight averaging, our scheme is expected to dynamically adapt the window size in an efficient manner even for non-homogeneous reading patterns in mobile environments. In addition, we analyze reading patterns in the window and effects of decreased window so that a more accurate and efficient decision on window adaption can be made. With our scheme, we can expect to obtain the ultimate goal that RFID middleware systems can provide applications with more clean data so that they can ensure high quality of intended services.

• Journal of Korean Home Economics Education Association
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• v.22 no.2
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• pp.101-113
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• 2010

The purpose of this study is to develop and evaluate the practical problem-based lesson plan for high school home economics class focusing on consumer choice of genetically modified(GM) food. The ADDIE instructional design model was applied. The first analysis step, practical problems were chosen based on the previous studies and the educational goal suggested in the 2007 revised curriculum. The next design and development steps, 4 hours of lesson plans were developed by reconstitution a chapter of Food Safety. Additional learning materials(17 student activity papers, 17 student reading materials, 3 teacher reading materials) were also developed. The implementation step, the lesson plans developed were applied to the S high school students in Bundang, Geyonggi-do. The last evaluation step, the perception change about GM food and the adequacy of teaching method were evaluated. As results, the studens' attitudes and minds towards the GM food were changed. The level of concern on GM food increased, and the ratio of students who would check whether GM food or not was increased. Also students showed high level of satisfaction on teaching method and materials.

• International Journal of Internet, Broadcasting and Communication
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• v.10 no.3
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• pp.26-34
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• 2018

In this paper, we propose a scheduling scheme that can efficiently serve I/O requests having deadlines in flash storage. The I/O requests with deadlines, namely, real-time requests, are assumed to be issued for streaming services of continuous media. Since a Web-based streaming server commonly supports downloads of HTMLs or images, we also aim to quickly process non-real-time I/O requests, together with real-time ones. For this purpose, we adopt the well-known rate-reservation EDF (RR-EDF) algorithm for determining scheduling priorities among mixed I/O requests. In fact, for the use of an EDF-style algorithm, overhead of task's switching should be low and predictable, as with its application of CPU scheduling. In other words, the EDF algorithm is inherently unsuitable for scheduling I/O requests in HDD storage because of highly varying latency times of HDD. Unlike HDD, time for reading a block in flash storage is almost uniform with respect to its physical location. This is because flash storage has no mechanical component, differently from HDD. By capitalizing on this uniform block read time, we compute bandwidth utilization rates of real-time requests from streams. Then, the RR-EDF algorithm is applied for determining how much storage bandwidth can be assigned to non-real-time requests, while meeting deadlines of real-time requests. From this, we can improve the service times of non-real-time requests, which are issued for downloads of static files. Because the proposed scheme can expand flexibly the scheduling periods of streams, it can provide a full usage of slack times, thereby improving the overall throughput of flash storage significantly.

• The Journal of Korean Academic Society of Nursing Education
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• v.7 no.1
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• pp.126-142
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• 2001

Although a call for the implementation of PBL in nursing education is getting increased, it has not been actively implemented as it could be. The main reason for this situation seems to be the lack of well designed learning packages. Well designed PBL packages can be the core factor for the successful implementation of PBL. However, this seems to be the hardest task for teachers wanting to implement PBL. Therefore, the purpose of this study is to develop a systematic framework of PBL package development process and provide the examples of its application. This framework of the process of PBL package development includes thirteen steps. First of all, the team needs to decide a topic to be explored in the package and then clusters concepts related to the topic. Second, the team selects a real situation and writes it as a story. Third, knowledge, skills, and attitudes that practitioners need to know to deal with the situation will be explored. Fourth, learning objectives will be written. The next, the team will check if the situation includes multidisciplinary concepts and content. Sixth, the story will be divided into several parts. Seventh, part 1 will be written. Eighth, clinical documents related to part 1 need to be prepared. Ninth, the team will write a suggested approach for students. Then, they need to prepare a tutor's guide for part 1. Eleventh, the team will prepare a list of reading materials and plan for lectures and clinical laboratory sessions. Twelfth, they will write part 2 ~ part N following the steps from the seventh to the eleventh. The last step is evaluating the package and amending it as needed. These thirteen steps are very detailed and easy to follow for beginners. It is expected that this framework will contribute to accelerate the implementation of PBL in nursing education.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.9
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• pp.1845-1850
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• 2009

Generally, small and medium scale enterprises have conventionally been performing diligence and indolence management by hand, but many of them have been recently costing a lot of money for their diligence and indolence management and security maintenance. But yet, they have annoying sides due to the initial stage cost for the introduction of the system which is consisted of a terminal for reading a card, an RFID card, an administrative sewer and an application program for the diligence and indolence management as well as the insufficiency of the fixing skill being able to cope with the problems originating from hardware and software troubles. For this reasons, we developed a new diligence and indolence management system that the initial stage cost is moderate because it is needless to purchase a new server and to issue a new card, and the operation and management of the system is convenient because an RFID card reader communicates with a central administrative server in IDC(Internet Data Center) over internet for the diligence and indolence management.

• Journal of Microbiology and Biotechnology
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• v.24 no.4
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• pp.545-555
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• 2014

It has been previously demonstrated that laccases exhibit great potential for use in several industrial and environmental applications. In this paper, two laccase isoenzyme genes, lccB and lccC, were cloned and expressed in Pichia pastoris GS115. The sequence analysis indicated that the lccB and lccC genes consisted of 1,563 and 1,584 bp, and their open reading frames encoded 520 and 527 amino acids, respectively. They had 72.7% degree of identity in nucleotides and 86.7% in amino acids. The expression levels of LccB and LccC were up to 32,479 and 34,231 U/l, respectively. The recombinant laccases were purified by ultrafiltration and $(NH_4)_2SO_4$ precipitation, showing a single band on SDS-PAGE, which had a molecular mass of 58 kDa. The optimal pH and temperature for LccB were 2.0 and $55^{\circ}C$ with 2,2'-azinobis-[ 3-ethylbenzthiazolinesulfonic acid (ABTS) as a substrate, whereas LccC exhibited optimal pH and temperature at 3.0 and $60^{\circ}C$. The apparent kinetic parameters of LccB were 0.43 mM for ABTS with a $V_{max}$ value of 51.28 U/mg, and the Km and $V_{max}$ values for LccC were 0.29 mM and 62.89 U/mg. The recombinant laccases were able to decolorize five types of dyes. Acid Violet 43 (100 g/ml) was completely decolorized by LccB or LccC (2 U/ml), and the decolorization of Reactive Blue KN-R (100 g/ml) was 91.6% by LccC (2 U/ml). Thus, the study characterizes useful laccase isoenzymes from T. versicolor that have the capability of being incorporated into the treatment of similar azo and anthraquinone dyes from dyeing industries.

• Korean Journal of Childcare and Education
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• v.8 no.2
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• pp.139-155
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• 2012

This study aimed to glean lessons for desirable early childhood physical education programs by contouring the current paradigms on body or physical activities in the area of early education for young children. It also aimed to analyze their postures while participating in daily activities. The research method for analysis of children's postures while doing daily activities was OWAS(Ovako Working Posture Analysing System) which has been extensively used in ergonomics. Components of data analysis have been redesigned on the basis of information gathered from OWAS with the help of ergonomic experts. The results showed different percentages of children's movements according to activities such as whole class meeting, small group activity and outdoor activity. Small group and outdoor activities can be considered the most desirable physical activities not only for cognitive and social development but also for physical development. And it is recommended to reconstruct the quality and nature of whole class meetings in order to coordinate the physical nature of young children.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2004.06a
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• pp.278-281
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• 2004

The $\alpha$1,6-mannosyltransferase encoded by Saccharomyces cerevisiae OCH1 plays a key role for the outer chain initiation of the N-linked oligosaccharides. A search for Hansenula polymorpha genes homologous to S. cerevisiae OCHI (ScOCH1) has revealed seven open reading frames (ORF100, ORF142, ORF168, ORF288, ORF379, ORF576, ORF580). All of the seven ORFs are predicted to be a type II integral membrane protein containing a transmembrane domain near the amino-terminal region and has a DXD motif, which has been found in the active site of many glycosyltransferases. Among this seven-membered OCH1 gene family of H. polymorpha, we have carried out a functional analysis of H. polymorpha ORF168 (HpOCH2) showing the highest identity to ScOCH1. Inactivation of this protein by disruption of corresponding gene resulted in several phenotypes suggestive of cell wall defects, including hypersensitivity to hygromycin B and sodium deoxycholate. The structural analysis of N-glycans synthesized in HpOCH2-disrupted strain (Hpoch2Δ) and the in vitro $\alpha$1,6-mannosyltransferase activity assay strongly indicate that HpOch2p is a key enzyme adding the first $\alpha$1,6-mannose residue on the core glycan Man$_{8}$GlcNAc$_2$. The Hpoch2Δ was further genetically engineered to synthesize a recombinant glycoprotein with the human compatible N-linked oligosaccharide, Man$_{5}$GlcNAc$_2$, by overexpression of the Aspergillus saitoi $\alpha$1,2-mannosidase with the 'HDEL” ER retention signal.gnal.

• Korean Journal of Veterinary Research
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• v.43 no.1
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• pp.129-137
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• 2003

Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failures in sows and respiratory problems in piglets. The nucleocapsid(N) protein, encoded by the open reading frame 7 (ORF7) gene, is known to be the most abundant and antigenic protein in PRRS virus. Therefore, it was suggested that the N protein could be a suitable candidate for the detection of PRRS virus-specific antibodies and diagnosis of PRRS. In the present study, the ORF7 gene encoding the N protein was cloned and expressed as a fusion protein with the glutathione S-transferase (GST) in Escherichia coli. The resulting GST-N recombinant protein was used as an antigen for an indirect sandwich enzyme-linked immunosorbent assay (i-ELISA). Expressed GST-N recombinant protein was migrated at 41 kDa and reacted with ORF7-specific monoclonal antibody by Western blotting. In order to increase the specificity of the ELISA for the detection of PRRS virus-specific antibodes, an i-ELISA was developed using an anti-GST antibody as a capture antibody. The sensitivity and specificity of developed i-ELISA were 92% and 96%, respectively. Based on these results, it was suggested that the i-ELISA is a simple and rapid test for screening a large number of swine sera for the anti-PRRS virus antibodies.