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Isolation, Identification, and Characteristics of Lactic Acid Bacteria for Production of Fermented Soymilk which Has Improved Sensory Quality (관능이 개선된 발효두유 제조를 위한 젖산균 분리·동정 및 특성)

  • Jung, Min-Gi;Kim, Su-In;Hur, Nam-Youn;Seong, Jong-Hwan;Lee, Young-Geun;Kim, Han-Soo;Chung, Hun-Sik;Kim, Dong-Seob
    • Microbiology and Biotechnology Letters
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    • v.44 no.1
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    • pp.74-83
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    • 2016
  • In order to improve the sour taste and foul odor of fermented soymilk, bacteria were isolated from kimchi and identified. Of the 89 bacterial strains isolated from kimchi, 3 isolates produced fermented soymilk with a sour taste and foul odor. The selected bacterial strains R53, R83, and R84 were identified by morphological, biochemical, and 16S rRNA analyses as Weissella koreensis. The strain R83, which produced fermented soymilk having the mildest sour taste and foul odor, was selected for further investigation and named W. koreensis KO3. The optimum culture condition for the fermentation of soymilk by W. koreensis KO3 was at $30^{\circ}C$ for 12 h. When soymilk was fermented under the optimum culture conditions, the viable cell count reached up to $8.71{\times}10^8CFU/ml$ and pH and acidity reached as low as 6.02 and as high as 0.33%, respectively. Twenty-seven amino acids and their derivatives were detected in fermented soymilk. The amounts of serine, glycine, threonine, alanine, and aspartic acid, which contribute to a sweeter taste, increased during fermentation. Orinithine, which was not detected before fermentation, increased during fermentation. Sensory evaluation showed that W. koreensis KO3-fermented soymilk has improved bean, roasted nut, and sour flavors as well as an enhanced mouthfeel, appearance, preferability, and overall acceptability compared with those of standard fermented soymilk. With further study and development, soymilk fermented by W. koreensis KO3 could serve as a health-promoting food with favorable sensory qualities.

Variation of Fresh Shoot Quality by Storage Temperature after Harvesting in Local Strain of Sedum sarmentosum (돌나물 수집계통의 수확후 저장온도에 따른 품질변이)

  • Kim, Hyo-Jin;Bae, Jong-Hyang;Lee, Seung-Yeob
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.240-246
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    • 2007
  • To evaluated the effect of low temperature storage on quality of fresh shoot of Sedum sermentosum, weight loss, Hunter L, a and b value, chlorophyll, water content, and general appearance were investigated during storage at 20, 10, and $5^{\circ}C$. Just after harvesting, fresh shoots ($10{\pm}2cm$ length) were stored with wrap film (linear LDPE)-packaged condition using the Styrofoam dish ($20{\times}10cm$, which 40 fresh shoots per dish). The fresh weight of 40 shoots was gradually decreased according to days after storage and higher storage temperature. The weight loss of fresh shoots showed significant difference (8.7-25.3%) between just before storage and 10 days after storage, and the degree of weight loss was more severer in higher storage temperature. Wanju local strain showed the lowest weight loss, and the most severe case was Pohang local strain. SPAD value related to chlorophyll content was rapidly decreased after 4 and 6 days at 20 and $10^{\circ}C$ storage, respectively. It was slowly decreased according to days after storage at $5^{\circ}C$. Yellowish color in bottom leaves of shoot appeared after 4, 6 and 8 days at 20, 10, and $5^{\circ}C$ storages, respectively. Hunter L and a values were gradually increased after storage, and it showed significant difference between just before storage and 10 days after storage. The total rate of discoloration over 50% or decay shoot was largely increased as higher temperature. It was significantly correlated to the water content of fresh shoot among local strains $(P{\leq}0.01)$. The freshness of shoot after storage was maintained in order of Wanji, Gunsan, Wando and Pohang local strain. The results indicated that the freshness was maintained for 8 days after $5^{\circ}C$ storage, when the fresh shoot was stored with wrap film-packaged condition.

A Study on the Solubilizing and Emulsifying Action of Tocopheryl Acetate using Plant Surfactant (식물성계면활성제를 사용한 토코페릴아세테이트의 가용화와 유화력에 관한 연구)

  • Kim, In-Young;Bae, Bo-Hyeon
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.4
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    • pp.893-905
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    • 2020
  • This study is a study on solubilization and emulsifying power of tocopheryl acetate using vegetable surfactants. High purity polyglyceryl-10 isostearate and polyglyceryl-10 oleate were mixed to synthesize a vegetable surfactant with excellent solubilizing power and emulsifying power. The mixed raw material was named Solubil EWG-1100. The appearance of this raw material was a pale yellowish paste with a specific smell, specific gravity of 1.12, and acid value of 0.085. The HLB value of this surfactant was calculated by the Griffin's equation with an average value of 15.17. The behavior of this surfactant to solubilize tocopheryl acetate was mechanically verified. The performance of solubilization was evaluated by a method of visual evaluation and was measured by a transmittance rate at 650 nm using a UV spectrophotometer. As a result, in the formulation using 3% ethanol as a co-solvent, the concentration of surfactant was required to solubilize tocopheryl acetate was required about 5 times of natural surfactant. In the formulation without ethanol as a co-solvent, the concentration of surfactant was required to solubilize tocopheryl acetate required about 7 times of natural surfactant. In addition, the concentration of surfactant required to make an emulsifivation 10 % of tocopheryl acetate was 1 wt% of Solubil EWG-1100, and the emulsified particle size was 3.5 mm in cream formula. In order to obtain stable and fine emulsified particles, it was found that as the concentration of tocopheryl acetate increased, the concentration of Solubil EWG-1100 also was to increase. As a result of testing the solubilizing power of the surfactant according to the pH various change, it showed stable solubilizing power in the acidic region of pH=3.2, the neutral region of pH=7.0, and the alkaline region of pH=11.8. As application, based on these results, it is expected that it can be widely applied to the cosmetics field that develops skin care prescriptions, sensitive skin products, and heavy dry skin products.

Study on the Environment and Benthos in the Intertidal zone of Haenam peninsula, Korea (해남반도 조간대의 환경과 저서동물상에 관한 연구)

  • SHIN Sook
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.26 no.1
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    • pp.63-75
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    • 1993
  • We identified the benthos collected from intertidal zone in Sacho, Naedong, Oran and Kusong of Haenam peninsula in October, 1990 and May, 1991 and examined the distribution pattern on the bases of the analysis of community structure and the physicochemical analysis of sediment including the grain component. The identified benthos consisted of 141 species and 4,641 individuals. The number of species and individuals of Mollusca, Arthropoda and Annelida occupy more than $85\%$ of the total number. When the intertidal zone were divided into soft area and rocky area the species found in rocky area(114 species) were present in a more diverse way than that in soft area(69 species). The composition of species found in 4 localities turned out to be very similar in soft area while in rocky area the most diverse species were found in Kusong. The seasonal appearance frequency of species and individuals showed that the number collected in the spring(106 species, 3,002 individuals) was higher than that in the fall(85 species, 1,639 individuals). The most dominant species was Cerithideopsilla djadjariensis belonging to Gastropoda, Mollusca(412 individuals) and the species collected more than 200 individuals were 5 species of Mollusca and 1 species of Arthropoda. The species diversity index of 4 localities was in the order of Kusong, Oran, Naedong and Sacho revealed no significant differences among regions. The dominance index was low in Kusong and was high in Sacho. The index of interstational species similarity in soft sediment was very similar with $0.44{\sim}0.53$ value and the index was quite high in Kusong and Oran. This agrees very well with the similarity of the grain component of sediment. In case of rocky area the index was quite low with 0.27 for Kusong and Sacho situated far away from each other. Thus the present study suggests that the distribution of the species was subjected to the physicochemical environmental factor such as the grain component of sediment and the content of organic materials including the geographical factor.

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Hepatogenic Potential of Umbilical Cord Derived-Stem Cells and Human Amnion Derived-Stem Cells (사람의 제대 및 양막유래 줄기세포의 간세포로의 분화)

  • Kim, Ji-Young;Lee, Yoon-Jung;Park, Se-Ah;Kang, Hyun-Mi;Kim, Kyung-Sik;Cho, Dong-Jae;Kim, Hae-Kwon
    • Clinical and Experimental Reproductive Medicine
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    • v.35 no.4
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    • pp.247-265
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    • 2008
  • Objectives: Many types of liver diseases can damage regenerative potential of mature hepatocytes, hepatic progenitor cells or oval cells. In such cases, a stem cell-based therapy can be an alternative therapeutic option. We examined whether human amnion-derived mesenchymal stem cells (HAM) and human umbilical cord-derived stem cells (HUC) could differentiate into hepatocyte-like cells as therapeutic cells for the liver diseases. Methods: HAM and HUC were isolated from the amnion and umbilical cord of the volunteers after a caesarean section with informed consent. In order to differentiate these cells into hepatocyte-like cells, cells were cultivated in hepatogenic medium using culture plates coated with fibronectin. Effects of hepatocyte growth factor, L-ascorbic acid 2-phosphate, insulin premixture fibroblast growth gactor 4, dimethylsulfoxide, oncostatin M and/or dexamethasone were examined on the hepatic differentiation. After differentiation, the cells were analyzed by RT-PCR, immunocytochemistry, immunoblotting, albumin ELISA, urea assay and periodic acid-schiffs staining. Results: Initial fibroblast-like appearance of HAM and HUC changed to a round shape during culture in the hepatogenic medium. However, in all hepatogenic conditions examined, HUC secreted more amounts of albumin or urea into medium than HAM. Expression of some of hepatocyte-specific genes increased and expression of new genes were observed in HUC following cultivation in hepatogenic medium. Results of immunocytochemistry and immunoblotting analyses demonstrated that HUC secreted albumin into the culture medium. PAS staining further demonstrated that HUC could store glycogen inside of the cells. Conclusions: Both HUC and HAM could differentiate into albumin-secreting, hepatocyte-like cells. Under the same hepatogenic conditions examined, HUC more efficiently differentiated into hepatocyte-like cells compared with the HAM. The results suggest that HUC and HAM could be used as sources of stem cells for the cell-based therapeutics such as in liver diseases.

The Analysis for the Regulation related to Chinese Online Game Review -Focused on NHN Chinese Game- (중국 온라인 게임 심의에 관한 규제 분석 -중국 NHN 게임을 중심으로-)

  • Song, Seung-Keun;Kim, Chee-Yong
    • The Journal of the Korea Contents Association
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    • v.9 no.11
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    • pp.151-162
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    • 2009
  • The objective of this study is to uncover the standard and procedure of the review for the online game to export to China and try to find a plan how to solve a problem such as regulation for the online game imported into China depending on a protective policy for homemade game. The literatures related to the policy of China online game industry and the present condition of the wholistic restriction are reviewed and the standard and procedure of the review of China online game which is veiled was able to find definitely by using the case study on Korean company which was a great success as localization in China. As a result of this study, we found that the key factor on the review standard of China online game is the anti-social personality, such as 'the overthrow of Chinese socialist system', 'the territorial issue as the eviction of Hong Kong or the acknowledgment of Taiwan government', 'independence problem of the minorities', and 'damage to property as national treasure', et cetera. While the review of the homemade game in China takes about a month through just two steps in review process on online game, that of foreign game is required over at least three months through four steps in it. Moreover, it is difficult for foreign games to obtain the license due to total amount regulation for online game by 'General Administration of Press and Publication of the People's the Republic of China'. In the case of the joint-venture company, it is most important to the smooth communication between Korean company and Chinese company in order to overcome these strong regulation for online game review in China. Furthermore, as Chinese company in outward appearance it is need to be positioned absolutely in Chinese marketplace. The definite positioning strategy enables foreign companies to avoid the strong regulation about the foreign game in China. To understand, finally, deeply politics, society, and culture presented in Chinese ethics textbook permits the foreign companies to predict the exact standard of the review for online game. Therefore, this study is concluded with key implications to sustain guidelines on the deliberation for foreign companies which want to export online game to China to improve the predictability and accountableness about the review of the online game in China.

A study of conception of pyo(標).bon(本).joong(中) in the part of woongihak(運氣學) in negeong(內徑) (내경(內徑) 운기편(運氣篇)의 표(標).본(本).중(中) 개념에 대한 연구(硏究))

  • Baik, You Sang;Park, Chan-Guk
    • Journal of Korean Medical classics
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    • v.11 no.2
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    • pp.114-134
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    • 1998
  • The conception of pyo(標) bon(本) joong(中) in the part of woongihak(運氣學) of negeong(內徑) one of the important thing that decides the relation between six gi(六氣) and samyum and samyang(三陰三陽) or between each other's of samyum and samyang itself, it says that the relation of Pyo-rce(表裏). So this conception from the ancient times have been used to explain the theory of meridian(經絡) and organs(五臟六腑) and in other important field of oriental medicine - Sanghannon(傷寒論), it became basis of explanation of pcthoiogical principles in the system of six kyung(六徑). At first, the subject or this study is limited to the rament of $\ll$Somun(素問)$\gg$ in order to find the accurate and original meanings of pyo(標) bon(本) joong(中). And the meanings are studied by the way of expanding it's meaning with basic conceptions of woongihak(運氣學) and astronomy included in negeong(內徑). In this study, the results are summarized as the followings. 1. The contents of - the 68th chapter of negeong(內徑), concerning pyo(標) and joong(中) come under chogi(初氣) and joonggi(中氣) of the same chapter, after consideration of astronomical knowledge. And they become active during the period that last about 30days, a haft of one step(一步) of kaekgi(客氣). 2. Bon(本) as a kind of six gi(六氣) that is revealed from internal principle of something, that is to say Ohhaeng(五行), comes mainly under the kaekgi(客氣) of woongihak(運氣學) with the meaning of 'sign' is thai the specific properties of six gi(六氣) are revealed to our sight, so we can feel that through the change of nature, Joong(中) is the other property hidden in the inside of six gi(六氣), that is a portion of original nature(本性) like the bon(本). 3. The relation of pyo(標) and bon(本) is like that bctween the principle hidden inside in all things(理) and it's expression into the real world(氣) also similar to thai of yumyang(陰陽) and ohhaeng(五行). Therefore bon(本), though it means one of the six gi(六氣), hale the property of ohhaeng(五行) and pyo(標) is revealed, with an appearance of samyum-samyang(三陰三陰). 4. pyo(標) and joong(中) are also the both sides of yum(陰) and yang(陰) that revealed under the change of yumyang-ohhaengl(陰陽五行) in the nature. For example, if the one is yang(陰), the other is yum(陰). In the process that the change of all things is revealed out, first the property of pyo(標) appears strongly and then that of joong(中) appears comparatively weakly. But, in spite of the inhibitive relation of yumyang(陰陽), pyo(標) and joong(中) promote each other. 5. Under the course of change. It happens according to the bon(本), the property of ohhaeng(五行) in the case of soyang(少陽) and taeyum(太陰), because the effect of moisture(濕) and fire(火) that makes hyung(形) and gi(氣) is very strong in the universe. In the case of taeyang(太陽) and soyum(少陰), it happens according to the bon(本) and pyo(標) because they hare the polarity of water and fire(火水), at the same time, are not separated each other. In the case of yangmeong(陽明) and gualyum(厥陰), the change appears only according to the joong(中), but not strongly because the phase of yangmeong(陽明) and gualyum(厥陰) is a lull phase processing to the next one.

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Relationship between Movements of the Foot and Electromyographic Activities of Lower Leg Muscles in Young Women (젊은 여성의 발동작과 몇몇 하퇴근 근전도와의 관계)

  • Choe, Myoung-Ae;Shin, Dong-Hoon
    • The Korean Journal of Physiology
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    • v.18 no.1
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    • pp.81-96
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    • 1984
  • As the crippled persons work mostly in a sitting position and would be engaged in a foot-pressing job, it is necessary to assess their degree of participation of important muscles in various modes of foot activities. In this regard, it deems to be urgent to establish the reference standards for healthy persons. The present study has been undertaken to determine the degree of participation of the M. tibialis anterior, M. gastrocnemius and M. soleus in heel pressing, foot-flat pressing and forefoot pressing motion under varying forces, and in order to compare the electrical activities of three muscles with each other, and to analyse the time sequence between force and appearance or disappearance of EMG recording. Sixty-three healthy young women ranging from age of 18 to 23 were examined. The results obtained were as follows: 1. Participation of three muscles in foot movement under varying forces: A) Both gastrocnemius muscles or left soleus muscle did not contribute to heel pressing motion. Activity of both tibialis anterior muscles was the greatest among three muscles at heel pressing motion and the degree of their activities was proportional to force. B) Activities of left tibialis anterior muscle and both gastrocnemius muscles were negligible under 3 kg force at foot-flat pressing movement. Left gastrocnemius muscle did not contribute to foot-flat pressing under 6 or 9 kg force. Although activities of both soleus muscles and both tibialis anterior muscles were small, the degree of their activities increased with force at foot-flat pressing movement. C) Activities of both tibialis anterior muscles were negligible under 3 kg force at forefoot pressing motion. Activity of both soleus muscles was the greatest among 3 muscles and the degree of their activities increased with force at forefoot pressing motion. Both tibialis anterior muscles participated in forefoot pressing motion with severe exertion. 2. Electrical activities by foot movement under varying forces : A) Electrical activities were prominent in both tibialis anterior muscles and the level of their activities was linear with force at heel pressing motion. The degree of participation of both soleus muscles was small at heel pressing motion. B) Electrical activity of tibialis anterior muscle was the greatest among 3 muscles at foot-flat pressing movement and was followed by that of soleus muscle. Level of electrical activities increased with force in left soleus muscle and right tibialis anterior muscle at foot-flat pressing movement. C) Electrical activity of both soleua muscles was the greatest among 3 muscles at forefoot pressing movement and that of tibialis anterior muscle was next to soleus muscle. Level of electrical activities was proportional to force in left tibialis anterior muscle, right gastrocnemius muscle and both soleus muscles at forefoot pressing movement. 3. Time between starting signal and initiation of contraction of heel pressing and forefoot pressing motion in 3 muscles was longer than that of foot-flat pressing movement. Time of relaxation in 3 muscles was longer than that of contraction under varying forces. EMG recording appeared before initiation of contraction in both tibialis anterior muscles at heel pressing motion and in both soleus muscles at forefoot pressing movement under varying forces. Time of initiation of contraction was similar in both sides of tibialis anterior muscles under varying forces and time of onset of contraction at foot-flat pressing motion was the shortest. 4. Forefoot pressing movement would be encouraged in paralysis of tibialis anterior muscle, while heel pressing motion would be encouraged in paralysis of triceps surae muscle.

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Studies on the mechanism in the induced to unfertilized eggs(male sterility of Silkworm) by protected environment during pupae period (용기의 보호환경에 따른 불수정란(웅성불임잠)의 유발기구에 관한 조사연구)

  • 윤종관;오준식
    • Journal of Sericultural and Entomological Science
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    • v.15 no.2
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    • pp.9-14
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    • 1973
  • In order to find out effects of the generative power of silk worm moth which have been brought up in the high temperature accommodation at their pupa stage. For this specific study, 9 different kinds of male silk worms have been selected as specimen. All those specimen were brought up in the normal temperature at their larvae stage and after the pupation period they have been accommodated in the condition of high temperature for a certain length in accordance with the study programme. Afterwards, those mlae specimen were copulated with Suwon jam 103${\times}$104 which were all brought up in normal conditions. This study was carried out to find the copulation function as well as the ratio of unfertilized eggs(male sterility test). Results of study have been revealed as follows: 1) Although some differences were observed, male pura which have been brought up in the condition of high temperature shown the low rate of unfertilized eggs rather than those were brought up in the normal conpition. 2) In this group the eclosion(emergency) has been found to be poor rather, than those specimen brought up in normal conditions. 3) The copulation function of Moran, Daedong, J124 and C108 specimen were found to be poor than those of Suwon jam. 4) Fertility rate of Moran, Daedong, J124 and C108 was found to be around 65%. This figure is rather lower than what we normally expect. 5) Unfertilized egg ratio of Moran, Daedong and C108 were found to be around 20% if they were brought up in the condition of high temperature for one day from the time of pupation: 40% at 2 days, and 70% at 3 days duration. More than 3 days treatment has shown no progress in the unfertilized egg ratio. 6) One day's treatment for the pupa at the later stage has shown the unfertilized egg ratio of about 10%; 20% at 2 day's treatment, 35% at 3 day's treatment, 40-60% at 4 day's treatment, more than 60% at 5 day's treatment, and the 70% of fertilized egg ratio was only observed when the treatment days come to 7 days. It was understood that the unfertilized egg ratio was high at the antepupa stage rather than that of post-pupa stage. 7) According to the result of observation the sperm in copulatory pouch and seminal receptacle out of the normal female silk worm which have been copulated with the male brought up in the condition of high temperature at their moth stage. The reproduction system found in the control zone has been found to be normal and the sperm is amountful and active in motion while the sperm found in the treatment to be limited in amount and slow in motion. The observation was made within 5 hours from the copulation. 8) According to the result of observation of sperm of seminal receptacles of the female silkworm moth, and according to that observation of sperm in the seminal receptacle in female silkworm moth, the amount of sperm and mobility in the female moth brought up in high temperature were poor comparing that were brought up in normal temperature zone. Some of them are even found to be no trace of such. 9) Appearance and mosle of the copulatory organ of the male silkworm moth was found to be no anatomical change. 10) Testis of the later pupa stage which was brought up in the high temperature was found to be almost net developed to anucleate sperm and they are degenerated at stage of between maturation division and sperm abnormal stage. Mean time at control zone, the formation of anucleate sperm was already observed.

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Determine the effects of drying temperature on the quality change and antioxidant activity characteristics of blueberry (건조 온도에 따른 블루베리의 품질변화 및 항산화특성)

  • Shin, Dong-Sun;Yoo, Yeon-Mi;Kim, Ha-Yun;Han, Gwi-Jung
    • Food Science and Preservation
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    • v.22 no.4
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    • pp.505-511
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    • 2015
  • This study was performed to investigate the effect of drying temperature on the quality characteristics and antioxidant activity of blueberry. Blueberries were dried at different times and temperatures, including A ($40^{\circ}C$, 72 hr), B ($40^{\circ}C$, 48 h and $50^{\circ}C$, 24 hr), C ($50^{\circ}C$, 72 hr), and D ($60^{\circ}C$, 72 hr). The yield and pH ranges of blueberry were determined to be 17.73~31.17% and 3.20~3.25, respectively. The yield rate of A treatments, soluble solid of D treatments, was the highest. The yield rate and soluble content was the highest in the treatment of A and D, respectively. The moisture content and water activity were significantly decreased with the increase in drying temperature (p<0.05). The L value of A treatment, and a and b values of D treatment were the greatest. In the analysis of texture analyzer, hardness, springiness, cohesiveness, gummiess and chewiness were significantly increased with the increase in drying temperature (p<0.05). In the sensory evaluation of blueberry, the appearance and color were the highest in the A treatment. The moisture, texture and taste was highest in the B treatment. The overall acceptability was in the order of B > A > C >D. The total polyphenol content and DPPH radical-scavenging activity were 9.21~13.05 mg/GAEg and 61.90~81.42%, respectively, which were significantly decreased with the increase in drying temperature (p<0.05). Therefore, the optimum time and temperature for blueberry drying was founded to be B treatment ($40^{\circ}C$, 48 hr and $50^{\circ}C$, 24 hr) among other treatments.