• Applied Biological Chemistry
• /
• 제38권6호
• /
• pp.522-527
• /
• 1995

흑조위축병 바이러스(RBSDV)에 대한 antiviral agent를 개발하기위하여 RBSDV 게놈 조각 3번을 절단하는 망치머리형 구조의 라이보자임을 설계하였다. 라이보자임과 기질의 oligonucleotides는 DNA 합성기로 합성 후 서로 합치고, pBluescript KS(+)에 삽입하였다. 라이보자임과 기질 RNA는 $T_3$ RNA polymerase로 기내 전사하여 각각 193과 183 nucleotide의 RNA를 얻었다. 기질 RNA는 라이보자임 RNA에 의해 $55^{\circ}C$에서 2개의 조각으로 절단되었으나 $37^{\circ}C$에서는 절단되지 않았다. 또한 RBSDV의 3번 조각 RNA도 동일한 라이보자임에 의해 절단되었다. 이러한 결과로 합성된 헤머해드 라이보자임은 기내 절단 능력을 가지며 형질전환 식물체에서 antiviral agent로 이용될 수 있을 것이다.

• Archives of Pharmacal Research
• /
• 제26권11호
• /
• pp.887-891
• /
• 2003

Novel trans-2,2-dimethylcyclopropyl nucleosides were synthesized as potential antiviral agents. The key intermediate, 3, was synthesized via five steps from ethyl chrysanthemate and condensed with purine bases using the Mitsunobu reaction to give six cyclopropyl nucleosides. These synthesized nucleosides did not show any significant antiviral activity against HSV-1, HSV-2, EMCV, Cox B3, or VSV, at concentrations up to 100 $\mu M$.

• 한국미생물·생명공학회지
• /
• 제21권5호
• /
• pp.399-405
• /
• 1993

For screening of the antiviral agent from soil, about 520 strains of microorganisms were evaluated for their antiviral activity, About 6.9% of strains showed more than 95% antiviral activity against Herpes Simples Virus (HSV)-1. Two strains among 30 strains active against HSV-1 virus showed a quite strong activity against human immunodeficiency virus.

• Journal of Pharmaceutical Investigation
• /
• 제20권4호
• /
• pp.205-208
• /
• 1990

1-Dodecylazacycloheptan-2-one (Azone) is a new agent that enhances the percutaneous penetration of a number of different chemicals. BVDU and FEAU were evaluated for their potential efficacy in the treatment of cutaneous herpes simplex virus infections by in vitro studies through hairless mouse skin. This study demonstrates the value of penetration enhancing agent (Azone) and the need for a predictable evaluations in the development of topical antiviral agents.

• 약학회지
• /
• 제34권6호
• /
• pp.429-433
• /
• 1990

Transdermal patches and polymer membrane were prepared and evaluated for their ability to antiviral agent in vitro. The membrane perpared with styrene and HEMA by 0.5 and 10% of styrene composition. And the transdermal patches were fabricated with this membrane and silastic silicone sheeting. The antiviral agents used were ACV, BVDU and FEAU. The higher HEMA content membranes exhibited relatively high release rate of each drug. Permeation was enhanced by increasing of drug concentration. The parameters of each drug in diffusion experiment with styrene-HEMA membrane were investigated.

• Bulletin of the Korean Chemical Society
• /
• 제31권9호
• /
• pp.2473-2478
• /
• 2010

Novel 1'-methyl-5'-norcarbocyclic adenosine phosphonic acid analogues were synthesized using an acyclic stereoselective route from commercially available 3,3-diethoxy-propan-1-ol 4. The synthesized nucleoside phosphonate 19 and phosphonic acid 21 were subjected to antiviral screening against various viruses.

• 생약학회지
• /
• 제50권1호
• /
• pp.1-10
• /
• 2019

Recently, companion animal culture has grown rapidly and mature, raising interest in preventing and treating animal diseases. In particular, viral infection was a serious threat to companion animal health because there was no proper antiviral drugs. Synthetic antiviral drugs have limitations such as low efficiency, toxicity, and occurrence of resistant viruses. Therefore, attempts to find new anti-viral drugs from natural sources have continued. This review focused on the natural products and active substances that exhibit antiviral activity against three viruses: canine distemper virus (CDV), canine parvovirus (CPV), and feline calicivirus (FCV) that cause fatal diseases in dogs and cats. Natural plant extracts, flavonoids, polysaccharides, alkaloids and saponins showed antiviral activity with various mechanisms and differences in activity depending on the structure. Especially, quercetin and epigallocatechin-3-gallate (EGCG) showed antiviral activity through a multi-mechanism that interferes with the attachment and penetration stages of the virus and inhibits the viral polymerase within the cell. Some natural plant extracts showed a virucidal activity and showed the potential effect as a preventative agent to prevent the viral infection. This review is expected to provide research trend on the development of antiviral natural products for companion animals.

• Archives of Pharmacal Research
• /
• 제23권6호
• /
• pp.559-563
• /
• 2000

Novel exomethylene cyclopropyl nucleosides were synthesized as potential antiviral agents. The key intermediate 5 was synthesized in 4 steps, from Feists acid 1 and was condensed with purine derivatives by the $S_N2$ type reaction to give some cyclopropyl nucleosides. The synthesized nucleosides did not showed any significant antiviral activity against HSV-1, HSV-2, HCMV, HIV-1, HIV-2, and HBV up to 100 $\mu\textrm{m}$.

• 약학회지
• /
• 제50권2호
• /
• pp.65-69
• /
• 2006

Novel 4'-hydroxymethyl branched thioapiosyl nucleosides were synthesized in this study. The introduction of hydroxymethyl group in the 4'-position was accomplished by a [3,3]-sigmatropic rearrangement. Thioapiosyl sugar moiety was constructed by sequential ozonolysis, reduction and cyclization. The pyrimidine nucleosidic bases (uracil, 5-fluorouracil, 5-iodouracil, 5-chlorouracil, 5-bromouracil) were efficiently coupled by Vorbruggen glycosyl condensation procedure (per-silyated base and TMSOTf). The antiviral activities of the synthesised compounds were evaluated against the HIV-1, HSV-1, HSV-2 and EMCV 5-Iodouracil 18 showed weak antiviral activity against HSV-1 $(EC_{50}=30.7{\mu}M)$.

• 생약학회지
• /
• 제30권1호
• /
• pp.34-39
• /
• 1999

To search for less toxic antiherpetic agents, the inhibitory effects of twelve kinds of flavonoids including chrysin, quercetin, quercitrin, rutin, fisetin, gossypin, kaempferol, morin, naringenin, naringin, hesperetin and hesperidin on the plaque formation of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in Vero cells were examined by plaque reduction assay in vitro. Some flavonoids tested in this study showed potent antiherpetic activity, reducing intracellular replication of herpes simplex viruses when Vero cell monolayers were infected and subsequently cultured in medium containing flavonoids. Naringenin showed the most potent antiviral activity against HSV-1 with selectivity index (SI) of 19.1 and hesperetin showed the most potent antiviral activity against HSV-2 with SI of 9.8. These results suggest that some flavonoids may be a potential therapeutic agent for the treatment and prevention of herpes simplex virus infections.