• Title/Summary/Keyword: Antioxidative components

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Chemical Components in Stalks and Leaves of Sasa borealis Makino and Antioxidative and Antimicrobial Activities of Extracts (조릿대 줄기와 잎의 화학성분과 그 추출물의 항산화 및 항균활성)

  • Ko, Myung-Soo
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.125-132
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    • 2008
  • This study investigated the cremical components of 1he s1aks anI leaves of Sasa borealis Makino, and the antioxidative activities, nitrite scavenging levels, and antimicrobial activities of extracts of the stalks and leaves. The moisture contents of stalks and leaves were 59.87%(w/w) and 28.79%(w/w), the crude protein levels 2.09%(w/w) and 6.33%(w/w), the crude fat concentrations 1.21%(w/w) and 3.43%(w/w), and the ash levels 0.99%(w/w) and 3.76%(w/w), respectively. The major mineral components were K, Na, Mg and Mn; and the K contents of stalks and leaves were the highest amongst the minerals tested, at 350.27 mg% and 639.60 mg%, respectively. The principal organic acids of stalks and leaves were acetic acid, citric acid and succinic acid, and the organic acid content of leaves was higher than that of stalks. The antioxidative activity of stalk extracts was higher than that of leaves, and the antioxidative activities of ethanol extracts were higher than those of hot water extracts. The 0.5%(v/v) ethanol stalk extracts showed stronger antioxidative effects than did the 0.02%(w/v) butylated hydroxyanisole. All extracts showed nitrite scavenging activities, and hot water extracts from stalk showed the highest activities. Hot water extracts showed antimicrobial activities against all bacterial strains tested, and ethanol extracts from leaf showed strong antimicrobial activities against most bacteria, except Listeria monocytogenes and Salmonella typhymurium. The antimicrobial activities of most stalk and leaf extracts were higher than those obtained using 1.0%(w/v) sorbic acid.

Identification of Phenolic Antioxidative Components in Crataegus pinnatifida Bunge (산사 항산화성 물질의 분리 및 동정)

  • Kim, Jeong-Sook;Lee, Gee-Dong;Kwon, Joong-Ho;Yoon, Hyung-Sik
    • Applied Biological Chemistry
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    • v.36 no.3
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    • pp.154-157
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    • 1993
  • Based upon the antioxidative effectiveness of ether extracts of defated Crataegus pinnatifida B., phenolic antioxidative components were separated by gel column chromatography and identified by MS and H-NMR. Two or three individual compounds were found in free, soluble and insoluble bound phenolic acids, respectively and they were identified as caffeic acid, protocatechuic acid, phloroglucinol and pyrogallol.

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Constituents Analysis of Amino Acid and Antioxidative Activity from Cultivated Callus and Rhizome in Rhodiola sachalinensis

  • Song, Won-Seob;Chi, Hyung-Joon;Rim, Yo-Sup;Yoon, Jae-Ho
    • Plant Resources
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    • v.5 no.1
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    • pp.78-85
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    • 2002
  • The material of Rhodiola sachatinensis collected from an alpine region of the west-northern China. For analysing the effect, 1 used Rhodiola sachatinensis's rhizome and cultivated callus. In EtOAc, BuOH, $H_2O$ separation the plant showed strong antioxidative activity, but not in Hexane. The radical scavenging effect of EtOAc(RC$_{50}$,35(g), BuOH(RC$_{50}$, 43(g), H$_2$0(RC$_{50}$, 50(g) fraction and MeOH extract(RC$_{50}$, 50(g) of the Rhodiola sachatinensis was comparable to that of synthetic antioxidant BHA(RC$_{50}$, 14(g) and $\alpha$-Tocopherol(RC$_{50}$, 12(g). Total amino acid concentration of plant of In nature condition were 18,009ppm, and major components were arginine, glutamic acid, aspartic acid and valine. The ratio of essential/total amino acid on plant of In nature condition was 46.93%. Total amino acid concentration of callus of In vitro condition were 32,435ppm, and major components were valine, histidine, lysine and leucine. The ratio of essential/total amino acid on callus of In vitro condition was 56.07%. was 56.07%.

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Effect of Cynanchum wilfordii Hemeseley Extract on antioxidative enzyme in rat PC 12 pheochromocytoma.

  • Sung, Mi-Kyung;Kwon, Hye-Soon;Lee, Eun-Ju
    • Proceedings of the KSCN Conference
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    • 2003.05a
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    • pp.143-143
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    • 2003
  • Reactive oxygen species are highly reactive oxidant molecules and react with cellular components, causing oxidative damages. These damages may play a significant role in the causation of several chronic diseases. Antioxidative defence systems are present in the body to protect cells from oxidative damages. The first line of defence include dietary antioxidants and enzymes such as superoxide dismutase. Cynanchum wilfordii Hemeseley has been used for centuries as a tonic nutraceutical in China and Korea. (omitted)

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Effects of Gamma Irradiation on Lipid Components, Antioxidative Enzyme Activities and ${\alpha}-Tocopherol$ in Beef (감마선 조사가 우육의 지질성분과 항산화 효소 활성도 및 ${\alpha}-Tocopherol$에 미치는 영향)

  • Yook, Hong-Sun;Kim, Seung-Ai;Chung, Young-Jin;Kim, Jong-Goon;Chung, Cha-Kwon;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.31 no.1
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    • pp.252-256
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    • 1999
  • Effects of gamma irradiation on lipid components, antioxidative enzyme activities and ${\alpha}-tocopherol$ in beef (M. Semitendinosus and Longissimus dorsi) were investigated. The lipid components (total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride and phospholipid) and the antioxidative enzyme activities (glutathione sulfur transferase, catalase and total superoxide dismutase) were not significantly changed by gamma irradiation up to 10 kGy (p>0.05). However, the contents of ${\alpha}-tocopherol$ in Longissimus dorsi significantly decreased with irradiation dose (P<0.05).

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Studies on the Antioxidative Activities and Active Components of the Extracts from Pleurotus ostreatus (느타리버섯 추출물의 항산화 활성과 그 성분에 대한 연구)

  • Kim, Min-Hee;Jeong, Eun-Jeong;Kim, Yong-Suk
    • Journal of Food Hygiene and Safety
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    • v.31 no.2
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    • pp.119-125
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    • 2016
  • Antioxidative components and activities of the extracts from Pleurotus ostreatus extracted at different ethanol concentration were analyzed and their correlation were investigated. Ergothioneine, total phenolic compounds, and flavonoid contents of the extracts from P. ostreatus extracted with hot water (0% ethanol) were the highest ($2.98{\pm}0.05$, $9.51{\pm}0.45$, and $2.83{\pm}0.03mg/g$, respectively) and the contents were decreased according to increase of ethanol concentration for extraction. DPPH and ABTS radical scavenging activities of the extracts from P. ostreatus extracted with hot water were the highest ($80.41{\pm}0.56$ and $91.47{\pm}0.11%$, respectively). FRAP value also showed the highest reducing power by $8.86{\pm}0.33\;FeSO_4\;eq$. mM in hot water extracts. Positive correlations were found between ergothioneine contents and antioxidative active components and antioxidant activity of the extracts from P. ostreatus. Results indicate that hot water extraction was most efficient for the extracts with high antioxidative activities from P. ostreatus.

Isolation and Identification of Antioxidative Components from Bark of Rhus javanica Linne (붉나무 수피로부터 항산화 물질의 분리 및 동정)

  • Oh, Ji-Young;Choi, Ung;Kim, Yong-Suk;Shin, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.35 no.4
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    • pp.726-732
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    • 2003
  • The crude extracts from Rhus javanica Linne showed comparatively strong antioxidative activity in test oils. Antioxidative components were isolated and identified by column chromatography, thin layer chromatography, UV, and NMR. These antioxidative components were added to several oils to compare antioxidative activity with several commercial antioxidants, such as BHA, BHT, and tocopherol. After the sixth column chromatography, one fraction (R-18-9-3-2-4-2) was separated from chloroform layer of Rhus javanica Linne. The R-18-9-3-2-4-2 fraction was identified as methyl gallate by $^1H-NMR$ and $^{13}C-NMR$ and confirmed with methyl gallate standard as authentic. The R18-9-3-2-4-2 fraction from chloroform layer of Rhus javanica Linne showed stronger activity than that of the ${\alpha}-,\;{\delta}-tocopherol$, BHT, and BHA at the same concentration.

Quantitative Structure-Activity Relationship (QSAR) of Antioxidative Anthocyanidins and Their Glycosides

  • Chang, Hyun-Joo;Choi, Eun-Hye;Chun, Hyang-Sook
    • Food Science and Biotechnology
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    • v.17 no.3
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    • pp.501-507
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    • 2008
  • The quantitative structure-activity relationships (QSAR) study of antioxidative anthocyanidins and their glycosides were evaluated using 4 different assays of Trolox equivalent antioxidant capacity (TEAC), superoxide radical ($O_2^{{\cdot}-}$), hydrogen peroxide ($H_2O_2$), and peroxynitrite radical ($ONOO^-$) scavenging with TSAR software. Four models were developed with significant predictive values ($r^2$ and p value), which indicated that the antioxidant activities were mainly governed by the 3-dimensional structural energy (torsional energy), constitutional properties (the number of hydroxyl and methyl groups), and electrostatic properties (heat of formation, and dipole, quadrupole, and octupole components). This QSAR approach could contribute to a better understanding of structural properties of anthocyanidins and their glycosides that are responsible for their antioxidant activities. It might also be useful in predicting the antioxidant activities of other anthocyanins.

Studies on Biological Activity of Wood Extractives(XIII) - Chemical Components and Antioxidative activity of the leaves of Sophora japonica - (수목추출물의 생리활성에 관한 연구(XIII) - 회화나무잎의 화학성분(化學成分)과 항산화(抗酸化) 활성(活性) -)

  • Park, Youngki;Lee, Hak-Ju;Lee, Sung-Suk;Choi, Don-Ha
    • Journal of the Korean Wood Science and Technology
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    • v.31 no.5
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    • pp.43-48
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    • 2003
  • From the leaves of S. japonica, three compounds were isolated and identified as follows : hypogallic acid, kaempferol and quercetin. The free radical scavenging activities of the isolated compounds were 98.2%, 96.2% and 90.2% at 100 ㎍/ml, respectively. Compound 1 (hypogallic acid) and compound 2 (kaempferol) indicated higher antioxidative activity compared with reference compound, BHT.

Nutritional Components and Antioxidative Activities of Jujube (Zizyphus jujuba) Fruit and Leaf (대추 열매와 잎의 영양성분 및 항산화 활성)

  • Kim, Il-Hun;Jeong, Chang-Ho;Park, Soo-Jeong;Shim, Ki-Hwan
    • Food Science and Preservation
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    • v.18 no.3
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    • pp.341-348
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    • 2011
  • The nutritional components and antioxidative activities of jujube fruit and leaf were investigated and analyzed to provide basic data for functional food materialization and processing. The nitrogen-free extract contents of the fruit and leaf were 71.92% and 41.51%, respectively. The mineral components of the fruit and leaf were rich in Ca (72.14 and 3,252.09 mg/100 g), K (899.82 and 1,708.12 mg/100 g), and P (172.11 and 286.28 mg/100 g), respectively. The major free sugars of the fruit were glucose (13.01 %) and fructose (7.35%); and of the leaf, sucrose (3.94%) and fructose (0.75%). The ascorbic acid contents were higher in fruit (135.73 mg/100 g) than in the leaf (100.43 mg/100 g). The analysis of the component amino acid showed a relatively high ratio of glutamic acid, aspartic acid, proline, and essential amino acids of leucine, but a low methionine and cystine content. The ABTS and FRAP assays indicated that the butanol fraction of the leaf was a more potent radical scavenger and reducing agent than the other five solvent fractions. The butanol fraction of the leaf also presented inhibitory effects against lipid peroxidation in a dose-dependent manner. Therefore, this study verified that the butanol fraction of the leaf has strong antioxidative activities that are correlated with its high level of phenolics, particularly rutin and quercitrin. These phenolics of jujube leaf can be utilized as effective and safe functional food substances, i.e., natural antioxidants.