• 한국의류학회지
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• 제42권3호
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• pp.516-529
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• 2018

This study investigated the dyeing properties and bio-functions of cotton fabrics dyed with naturally fermented Ecklonia cava extract in order to compare it with a comparison of unfermented extract. Hot water-extracted Ecklonia cava was fermented naturally under the various conditions of a fermenting period (2-8 days) and amount of molasses (0.1-1.8% v/v); in addition, it was also tested for characterization by FT-IR, antioxidant activity, total polyphenol content, and anti-microbial activity. For dyed cotton fabrics, color strength (K/S), physical color properties, dyeing fastness, sun protective property, and anti-microbial activity were evaluated considering dyeing conditions. As a result, the fermented dye under fermentation condition of 0.1% v/v with molasses during 4 days was revealed as having a similar chemical structure to the unfermented one and showed a total polyphenol content with 32.88mg/g and better antioxidant activity than the unfermented one. As for dyed fabrics, the color strength value by K/S was the highest under the condition of 0.1% v/v of molasses during 4 days among all fermenting conditions. The dyed fabrics had a reasonably good fastness (except for light). Anti-microbial activity against K. pneumoniae was better for the fermented extract-dyed fabric especially with lower dye concentrations.

• 한국식품영양학회지
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• 제36권1호
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• pp.42-49
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• 2023

To enhance the efficacy of Abeliophyllum distichum leaves, extracts were prepared using different solvents for hydrolytic enzyme-treated Abeliophyllum distichum leaves. Physicochemical quality and antioxidant activity were measured. Soluble solids, reducing sugar, ascorbic acid, flavonoids, and polyphenols contents showed the lowest values in the control without enzyme treatment. However, they showed high contents in ethanol extract. In the case of enzyme treatment, their values were higher than those of the control. In particular, verbascoside content increased about 220 times more than that of the control group when treated with enzymes and extracted with 50% ethanol. pH was lowered upon enzymatic treatment. Regarding DPPH radical scavenging activity, for enzyme-free, 25% ethanol extract showed the highest activity among extracts with different solvents. For cellulase and pectinase-treated leaves, water extract showed the highest DPPH radical scavenging activity among extracts with different solvents. For leaves treated with enzyme combination, 50% ethanol extract showed the highest DPPH radical scavenging activity among extracts with different solvents. Regarding ABTS radical scavenging activity, it was generally higher in the 50% ethanol extract than in the water extract and 25% ethanol extract. In particular, verbascoside content was increased when the extract was prepared by co-treatment with enzymes and 50% ethanol.

• 한국전기전자재료학회논문지
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• 제28권1호
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• pp.51-56
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• 2015

The object of this study is to obtain the optimum mix proportion of halogen free compound with flame resistance and, for the purpose, thermal/electrical characteristics test are conducted using compatibilizing agents, flame resistance agents, hydroxide aluminum, sunscreen, antioxidant and silicon oil on the base resin of linear low density polyethylene (LLDPE), Ethylene vinyl acetate copolymer (EVA). Existing compound method accompanies many requirements to be satisfied including a lot of addition of flame resistance agents, prohibition of impact on mixing capability with base and property and etc. In this study, different from the existing method, the optimum mix proportion is determined and experimented by adding nano clay. Oxygen index test shows no difference between specimens while T-6, T-9 shows oxygen index of 29[%] and 26[%], respectively. This is concluded that hydroxide aluminum, which is a flame resistance agent, leads low oxygen index. From UL94-V vertical flame resistance test, the combustion behavior is determined as V-0, Fail based on UL94-V decision criteria. Viscometry shows low measurements in specimens with separate addition of compatibilizing agents or nano clay. Volume resistivity test shows low measurement mainly in specimens without compatibilizing agents. Therefore, with the flame resistance compound shows better performance for thermal/electrical property and the optimum mix proportion are achieved among many existing materials.

• 한국자원식물학회지
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• 제23권1호
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• pp.14-18
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• 2010

rosmarinic acid 생산에 미치는 영향을 알아보기 위하여 5계통의 strain (13333, 15834, R1000, R1200, R1601)을 이용하여 조사한 결과 A. rhizogenes R1601이 가장 높은 72.9%의 모상근 유도율을 보였다. 모상근 유도시 모상근 발생 수와 길이 신장에 미치는 영향을 조사한 결과도 A. rhizogenes R1601으로 감염시킨 잎 조직에서 평균 5.4개의 모상근이 발생하였으며, 평균 2.0 cm의 길이 신장으로 가장 좋은 결과를 나타냈다. 다섯 계통의 A. rhizogenes감염으로부터 유도된 각각의 모상근의 생육은 서러 다른 양상을 보였으나 경향은 모상근유도 결과와 유사하였다. A. rhizogenes R1601 감염으로 유도된 모상근의 생육은 배양 2주후 건물중을 조사한 결과 13.5 g/l로 다른 계통에서 유도된 모상근 보다 생육이 양호하였으며, Rosmarinic acid 생산량도 22.6 mg/g D.W.로 다른 계통에 비하여 가장 높게 나타났다. A. rhizogenes R1601이 배초향 모상근 유도, 생육과 rosmarinic acid 생산에 가장 좋은 적합한 계통이었음을 알 수 있었다.

• 통합자연과학논문집
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• 제9권1호
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• pp.72-79
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• 2016

Hexane, ethyl acetate and methanol extracts of whole plant of Boerhavia diffusa were screened for phytochemical and biological activities. Qualitative phytochemical screening via colorimetric method and the quantitative estimation of phenolic and flavonoid content were performed. Antioxidant assay using DPPH scavenging method was studied. Antimicrobial screening of plant extracts was done by cup diffusion technique. Cytotoxic activity of B. diffusa was studied by brine shrimp bioassay and anthelminthic activity was evaluated in vitro in Pheretima posthuma. This study revealed B. diffusa as a source of various phyto-constituents such as alkaloids, glycosides, saponins, tannins, carbohydrates, cardiac glycosides, flavonoids and terpenoids. Quantitative estimation of total phenol was found to be maximum in BEE i.e. $29.73{\pm}0.88$, BME $19.8{\pm}2.02$ and in BHE $9.15{\pm}0.304mgGAE/g$. Similarly, the total flavonoid content was found to be $17.44{\pm}0.75$ in BEE, $14.43{\pm}0.23$ in BHE and 3.678 mg QE/g in BME. Ethyl acetate extract showed its antibacterial activity against all tested pathogens except Escherichia coli whereas Staphylococcus aureus and Salmonella Typhi were resistant to methanol and hexane extract. The zone of inhibition (ZOI) of ethyl acetate extract against S. Typhi and B. cereus was found to be 18 mm and 14 mm respectively. The MIC value of BEE in S. Typhi was $3.125{\mu}g/ml$ and in B. cereus was $12.5{\mu}g/ml$. The preliminary screening of anticancer property of B. diffusa i.e. BSLT in methanol was found to be $165.19{\mu}g/ml$. B. diffusa was also found to contain anthelmintic property. The study helped in further exploration of medicinal properties of B. diffusa by phytochemical screening and biological activities paving the path for study and investigation in this plant.

• 한국작물학회지
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• 제60권4호
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• pp.484-490
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• 2015

본 연구는 완두나물로 활용 가능한 유전자원을 선발하고, 그에 적합한 재배조건을 설정하고자 재배 온도 및 기간에 따른 완두나물의 재배특성 및 항산화 활성을 분석하여 완두를 이용한 채소용 나물 최적 재배 조건을 구명하고자 실시하였다. 1. 완두 유전자원 38점 중 완두 나물로 이용 가능한 자원으로 PI269803, PI343278, PI343283, PI343300와 PI343307을 1차적으로 선발하였다. 2. 1차 선발된 완두자원을 대상으로 완두나물 재배 조건을 설정하기 위하여 $20^{\circ}C$, $23^{\circ}C$와 $25^{\circ}C$에서 각각 7일간 재배하였다. 그 결과 전장과 배축장은 $20^{\circ}C$와 $25^{\circ}C$에 비해 $23^{\circ}C$에서 가장 높게 증가하였다. 3. 재배온도별 나물수율은 $23^{\circ}C$에서 가장 높게 나타났으며, 유전자원 PI269803와 PI343300이 다른 자원에 비해 높은 나물수율을 나타내었다. 4. 재배온도별 완두나물의 조단백질 함량을 분석한 결과 온도에 따른 조단백질 변화는 나타나지 않았다. 5. 재배 온도별 완두나물의 폴리페놀 함량과 자유 라디칼 소거능을 측정함으로써 항산화 특성을 조사하였다. 그 결과 PI343278와 PI343283에서는 재배 온도가 증가함에 따라 폴리페놀 함량과 자유 라디칼 소거능이 감소하는 경향을 나타내었다. 그러나 PI269803와 PI343300에서는 $20^{\circ}C$와 $25^{\circ}C$에 비해 $23^{\circ}C$에서 높은 폴리페놀 함량과 항산화 활성을 나타내었다. 6. 높은 나물수율과 항산화 특성을 보이는 PI269803과 PI343300은 완두나물 이용에 적합한 자원으로 예상된다.

• 한국식품과학회지
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• 제48권2호
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• pp.159-164
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• 2016

박력분 밀가루에 대한 감잎 분말 대체량을 0-4%로 달리하여 쿠키를 제조한 후 물리 화학적 품질특성, 산화방지 활성 및 소비자 기호도에 대한 실험과 조사를 진행하였다. 쿠키 반죽의 pH와 밀도는 감잎 분말 첨가량이 증가함에 따라 유의적으로 증가하였으며(p<0.05), 수분 함량은 유의적인 차이를 나타내며 감소하는 경향을 보였다(p<0.05). 쿠키의 퍼짐성과 손실률은 감잎 분말 첨가량이 증가함에 따라 유의적으로 감소하는 경향을 보였다(p<0.05). 명도($L^*$), 적색도($a^*$) 및 황색도($b^*$) 모두 감잎 분말의 첨가량이 증가함에 따라 유의적으로 감소하는 경향을 보였으며(p<0.05), 경도는 유의적으로 증가하는 경향을 보였다(p<0.05). 산화방지 활성을 나타내는 DPPH에 대한 전자공여능 및 ABTS에 대한 라디칼 소거능은 유의적인 차이를 나타내며 증가하였고(p<0.05), 두 지표 간의 상관관계가 매우 높은 것으로 나타났다. 소비자 기호도 검사 결과 색과 부드러운 정도는 1% 첨가군이 가장 높았고, 향과 맛은 대조군, 전체적인 기호도는 2% 첨가군이 가장 높았으나, 대부분 항목에서 대조군과 1% 및 2% 첨가군 사이에서 유의적인 차이가 발견되지 않았으며 감잎 분말의 최적 첨가량은 2%인 것으로 판단된다. 한편 본 연구는 활용 범위가 작아 대부분 폐기되는 농산폐기물인 감잎을 활용하여 고부가가치 상품인 감잎 쿠키를 상품화하는데 필요한 실험적 data를 제공하고 있으며, 따라서 이를 활용한다면 소비자들에게 기능성과 기호성 모두 가진 쿠키를 제공할 수 있을 것으로 생각된다.

• KSBB Journal
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• 제25권3호
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• pp.277-282
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• 2010

새송이 버섯 (Pleurotus eryngii) 재배배지에 글루탐산나트륨 (monosodium glutamate, MSG)을 혼합 첨가하여 재배한 자실체의 아미노산 및 $\gamma$-aminobutyric acid (GABA)함량 변화, 그리고 항산화 작용 등의 생리활성을 분석하여 새송이버섯의 기능성 향상 가능 여부를 조사하였다. 실험결과 MSG 첨가량이 증가함에 따라 arginine, glutamic acid, alanine, glycine 등 아미노산은 함량이 증가되었으며 histidine, serine, methionine, isoleucine, leucine, phenylalanine 등은 함량 변화는 거의 없었다. 한편 MSG 첨가량을 증가시킴에 따라 자실체의 GABA함량은 전체적으로 증가되었으며 MSG 6 g/bottle 첨가군에서 자실체내에 가장 높은 GABA함량 (1.18 mg/g)을 얻을 수 있었다. 또한 배지에 MSG첨가량을 증가시킴에 따라 자실체 추출물의 DPPH (1,1-diphenyl-2-picrylhydrazyl) radical 소거능 및 아질산염 소거능 (nitrite scavenging activity)도 증가됨을 알 수 있었다. 이러한 결과로부터 새송이버섯을 재배할 때 배지에 소량의 저렴한 MSG (~US $1/kg)를 첨가하면 아미노산 조성 및 항산화 작용 등 생리활성이 개선되는 고 기능성 새송이버섯 식품소재가 개발될 수 있을 것으로 사료된다.

• BMB Reports
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• 제38권2호
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• pp.167-176
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• 2005

Nuclear factor-E2-related factor 2 (Nrf2) is known as a key regulator of ARE-mediated gene expression and the induction of Phase II detoxifying enzymes and antioxidant enzymes, which is also a common property of many chemopreventive agents. In the present study, we investigated the regulatory role of different chemopreventive agents including sulforaphane (SUL), allyl isothiocyanate (AITC), indole-3-carbinol (I3C), and parthenolide (PTL), in the expression and degradation of Nrf2 and the induction of the antioxidant enzyme HO-1. SUL strongly induced Nrf2 protein expression and ARE-mediated transcription activation, retarded degradation of Nrf2 through inhibiting Keap1, and thereby activating the transcriptional expression of HO-1. AITC was also a potent inducer of Nrf2 protein expression, ARE-reporter gene and HO-1 but had little effect on delaying the degradation of Nrf2 protein. Although PTL and I3C could induce ARE reporter gene expression and Nrf2 to some extent, they were not as potent as SUL and AITC. However, PTL dramatically induced the HO-1 expression, which was comparable to SUL, while I3C had no effect. In addition, when treated with SUL and PTL, inhibition of proteasome by MG132 did not cause additional accumulation of Nrf2, suggesting the involvement of other degradation mechanism(s) in the presence of these compounds such as SUL and PTL. In summary, the results of our current study indicated that different chemopreventive compounds have different regulatory properties on the accumulation and degradation of Nrf2 as well as the induction of cellular antioxidant enzyme HO-1.

• 생약학회지
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• 제47권3호
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• pp.264-272
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• 2016

This study was carried out to evaluate the preventive effect of the Corni Fructus (SSU) 50 % EtOH extract (SSU-E50) against bisphenol A (BPA) toxicity in Leydig cells and improving testosterone deficiency syndrome in orchidectomized Sprague-Dawly (SD) rats. Antioxidant properties were measured by radical scavenging activity of SSU-E50 in ABTS assay and DPPH assay. Also, real-time polymerase chain reaction(real-time PCR) was performed to quantify the mRNA expression levels of antioxidant enzyme. SD rats were divided into eight group: normal, sham operation (Sham), orchidectomized (ORX), ORX treated with testosterone 1 mg/kg (Tes. 1), ORX treated with SSU water extract 100 mg/kg (SSU-A 100) and 300 mg/kg (SSU-A 300), ORX treated with SSU 50 % EtOH extract 100 mg/kg (SSU-E 100) and 300 mg/kg (SSU-E 300). On a comparative basis, the SSU showed better activity quenching ABTS with an IC50 value of 0.29 mg/ml and DPPH with an IC50 value of 0.33 mg/ml. Cell viability was evaluated by MTS assay as described not cytotoxic at the highest concentration of $500{\mu}g/ml$. Cytotoxicity of BPA showed in $200{\mu}M$, but definitely survived by treatment with SSU in Leydig cells. In addition, SSU increased the mRNA expression levels of antioxidant enzyme in BPA induced Leydig cells. Superoxide dismutase (SOD) level was slightly increased and malondialdehyde (MDA) level was decreased with SSU-A 100 in in-vivo. These results suggest that Corni Fructus extracts have the greatest property as a natural anti-oxidative and improves testosterone deficiency syndrome source.