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Influence of Different Strains of Agrobacterium rhizogenes on Hairy Root Induction and Rosmarinic Acid Production in Agastache rugosa Kuntze  

Kim, Jong-Se (Department of Biology, Chosun University)
Oh, Eun-Jeong (Department of Biology, Chosun University)
Lee, Sook-Young (Research Center for Oral Disease Regulation of the Aged, Chosun University)
Publication Information
Korean Journal of Plant Resources / v.23, no.1, 2010 , pp. 14-18 More about this Journal
Abstract
Rosmarinic acid, an ester of caffeic acid with 3,4-dihydroxyphenyl lactic acid, is one of the main active constituents of Agastache rugosa Kuntze and has an astringent property, antioxidant capacity, anti-inflammatory activity, antimutagenic ability, antimicrobial capacity, and an antiviral property. Five different strains of Agrobacterium rhizogenes differed in their ability to induce Korean mint (Agastache rugosa Kuntze) hairy roots and also showed varying effects on the growth and rosmarinic acid production in hairy root cultures. A. rhizogenes R1601 is the most effective strain for the induction (72.90%), growth (13.50 g/l) and rosmarinic acid production (22.60 mg/g) in hairy root of Korean mint. Our results demonstrate that use of suitable strains of A. rhizogenes may allow study of the regulation of rosmarinic acid biosynthesis in hairy root cultures of Agastache rugosa.
Keywords
Agastache rugosa Kuntze; Agrobacterium rhizogenes; Hairy root; Rosmarinic Acid;
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