• Asian-Australasian Journal of Animal Sciences
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• v.14 no.5
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• pp.677-679
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• 2001

Effect of Growell a herbomineral premix was evaluated on performance, organ weight, humoral immune response, tissue and serum trace element profile of boilers. Day old 50 Babcock broiler chicks were randomly divided in two groups (C and T) and reared on deep litter system for 6 weeks of age. Chicks from group C were given basal diet while chicks from T group were fed basal diet supplemented with Growell at the rate 0.35 g/Kg. The chicks were vaccinated with Lasota strain of NCDV at $4^{th}$ and $28^{th}$ day of age. The birds receiving Growell treatment had higher body weight with better feed conversion ratio as compare to that of control chicks. Growell treatment had significantly reduced per cent weight of spleen and kidney, whereas that of bursa was increased. There was no effect of treatment on relative weight of thymus. HI antibody titer against NCDV in Growell treated chicks were higher as compare to untreated chicks indicating better humoral immune status. Growell treatment had no effect on serum Fe and Zn concentration. Dietary supplementation of Growell had significantly increased iron content of liver, kidney and muscle; zinc content of kidney and muscle; copper content of kidney and muscle and Mn content of kidney. Growell treatment improved the body weight, FCR and humoral immune status of broilers. Similarly, deposition of trace minerals in various organs was also increased in comparison to control.

• The Journal of the Korean Society for Microbiology
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• v.7 no.1
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• pp.17-20
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• 1972

A strain of Brucella showing the characteristics of Br. suis was isolated from a 55 day-old aborated fetus of a German Shepherd and the cause of the abortion was considered due to brucellosis. A considerable number of dog sera collected at the slaughter house in Taegu city showed antibody titers of 1:80 or more to Br. suis, and serum from the aborted dog 1:320 to Br. suis in titer.

• Journal of fish pathology
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• v.17 no.3
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• pp.171-177
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• 2004

This study was performed to investigate the effects of prolonged immersion vaccination in a dilute vaccine on the farmed olive flounder, Pararlichthys olivaceus. Juvenile olive flounders were immersed in the several diluted ($10^0$, $10^{-1}$, $10^{-2}$ and $10^{-3}$) commercial edwardsiellosis bacterins (5㎎/ℓ) for 2min (short immersion, SI) and 24 h (prolonged immersion, PI). After immunization, fish were analyzed to investigate on non-specific (serum lysozyme and bactericidal activity), specific immune responses (antibody agglutinin titer) and resistances against Edwardsiella tarda infection. The PI of the commercial vaccine diluted with $10^{-1}$ and $10^{-2}$ showed better results over the untreated control in terms of serum lysozyme, bactericidal activities and resistance against E. tarda infection. These results suggest that the prolonged treatments of the diluted vaccine might be a new approach improving the vaccination potential.

• Journal of Preventive Medicine and Public Health
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• v.23 no.1 s.29
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• pp.57-64
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• 1990

Since the reservoir of leptospires organism is consisted of a broad spectrum of animals, the best method of prevention is vaccination. The clinical trial of leptospires vaccine conducted on human volunteer for its immunogenicity and safety. Summarized results are as following : 1. The Oral temperature among vaccinated group ranged from $36.7{\pm}0.46^{\circ}C\;to\;37.0{\pm}0.34^{\circ}C$, while in placebo injected group it ranged from $36.4{\pm}0.46^{\circ}C\;to\;36.7{\pm}0.53^{\circ}C$. There was no association between vaccination and fever (p>0.05) 2. Mild local reactions revealed in vaccinees were swelling (50-75% ), Redness($75{\sim}90%$), and induration ($25{\sim}40%$). Placebo injected group revealed only redness in 12.5% in 1st injection and 37.5% in second injection. The duration local reactions on injection site for th vaccinees and place groups disappeared within 48 hours. 3. Generalized Symptoms complained by the vaccinees were myalgia (25%), back pain(15%), headache (15%), pruritus(15%), and abdominal pain(10%), whereas placebo group complained of headache (25%), myalgia(12.5%), back pain(12.5%), pain in eyes(12.5%), abdominal pain(12.5%) pruritus (12.5%) and nausea(12.5%). 4. The serological test(MAT) of vaccinees showed geometric mean antibody titer as follows : a. L. icterohemorrhagiae lai 1 week after 1st vaccination : 22.45 1 week after 2nd vaccination : 111.23 3 week after 2nd vaccination : 266.64 b. L. canicola canicola 1 week after 1st vaccination : 24.62 1 week after 2nd vaccination : 123.92 3 week after 2nd vaccination : 276.55 c. L. icterohemorrhagiae copenhageni 1 week after 1st vaccination : 28.28 1 week after 2nd vaccination : 128.55 3 week after 2nd vaccination : 247.88 Whereas all of the place injected group showed below 1:20 titers. The sero-conversion rate of vaccinees were 100 percent.

• Journal of Microbiology and Biotechnology
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• v.15 no.4
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• pp.873-879
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• 2005

Red sea bream iridovirus (RSIV) obtained from infected rock bream was propagated by Bluegill fry-2 (BF-2) cell culture. The virus titer was determined as $10^{5.5}\;TCID_{50}/ml$ on confluent BF-2 cell monolayers. The integrin binding site of ORF 055L of infectious spleen and kidney necrosis virus (ISKNV) was selected for the construction of a primer to obtain the RSIV ORF 055L gene. The genes were amplified using RSIV gene lyzate by PCR. The homologies of the ORF 055L sequence of RSIV with ISKNV and rock bream iridovirus (RBIV) were approximately $96\%$ and $100\%$, respectively. DNA vaccine was constructed by cloning the ORF 055L of RSN into pcDNA 3.1 (+), containing a cytomegalovirus (CMV) promoter. For antibody production, pcDNA-055 DNA vaccine was injected to BALB/c mice. The production of antibodies against pcDNA-055 DNA vaccine was confirmed by the Western blot analysis. The antibodies produced by the pcDNA-055 DNA vaccine showed efficacy to neutralize the RSIV in the neutralization test in BF-2 cell culture.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.3
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• pp.410-415
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• 2002

Adrenal glucocorticoids, secreted by the stimulus of adrenocorticotropic hormone (ACTH), are catabolic hormones in the pig. The present study was conducted to find whether active immunization against ACTH would suppress cortisol secretion accompanied by an increased growth rate in growing-finishing barrows. ACTH was conjugated to keyhole limpet hemocyanin or human histone using glutaraldehyde or 3-maleimidobenzoic acid N-hydroxysuccinimide, under a 2 (ACTH vs no hapten)${\times}$2 (carrier)${\times}$2 (crosslinker) factorial arrangement of treatments. Cross-bred barrows weighing approximately 25 kg were injected with an ACTHcarrier or carrier only conjugate every 4th wk and slaughtered at approximately 110 kg body weight. Antibodies against ACTH were detected in serum, as determined by $[^{125}I]$ACTH-binding activity, in most animals immunized against the ACTH conjugate, but not in carrier only-injected animals, except for the animals which had received the hapten conjugated to histone via glutaraldehyde. The $[^{125}I]$ACTH-binding activity of serum increased after the second booster injection, but overall ACTH antibody titer was very low. Main effect was not detected not only for the carrier and crosslinker but for the hapten in serum cortisol concentration, ADG, loin muscle area, backfat thickness and longissimus muscle composition including fat and protein. In addition, bound $[^{125}I]$ACTH percentage had no relation to cortisol concentration or to any of the above growth-related variables. Results suggest that ACTH or its conjugates used in the present study were not immunogenically potent enough to affect the glucocorticoid secretion and thus the growth of the immunized pigs.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.12
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• pp.6208-6214
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• 2012

The most essential but missing components to understand and use toxic substances from marine microalgae are developing the fast, easy and economical determining technology for detecting it. In this paper we produced the antibodies against saxitoxin (STX). Mariculture keyhole limpet hemocyanin (mcKLH) and ovalbumin (OVA) were used as carrier proteins. mcKLH-STX conjugates were injected into the peritonial cavity of BALB/c mouse for immunization. After bleeding from mouse, anti-STX antiserum was isolated. Indirect enzyme-linked immunosorbent assays (ELISA) was performed to determine antiserum titer using the microtiter plate coated with free STX and OVA-STX. A goat anti-mouse IgG-phosphatase conjugate was used as secondary antibody to enable chromogenic reaction. Reactions of anti-STX antiserum were very specific on the OVA-STX and free STX. Sensitivity of anti-STX antiserum on STX was very high and STX detection limit was to be 64.9 ng/kg for indirect ELISA.

• Korean Journal of Veterinary Service
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• v.31 no.3
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• pp.331-338
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• 2008

The study was carried out to evaluate the diagnostic efficacy of the tube agglutination test (TAT), enzyme-linked immunosorbent assay (ELISA) and the 2-Mercaptoethanol agglutination test (2-MAT) to detect human brucellosis patients in Korea. We examined 87 serum samples of people in the rural farm areas where bovine brucellosis had been reported. People in this study were divided into seven groups- farmers and their families, veterinarians, veterinary quarantine workers, livestock health control officers, artificial inseminators, livestock traders and healthy control individuals. Among 87 people, 65 were males and 22 were females ranging in age from 13 to 72 years. Of 87 serum samples, ELISA detected 21.84%, TAT detected 11.50% and 2-MAT detected 8.05% Brucella positive sera. Brucella specific IgG ELISA antibody titer was recorder higher in the individuals between the ages of 50 and 65 years. The highest prevalence rate of brucellosis(29.4%) was recorded in the cattle farmers and their family members followed by quarantine veterinary office workers (25%) and practicing veterinarians 01.1%). The majority of the Brucella sero-positive individuals in this study had a history of direct contact with animals.

• KSBB Journal
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• v.32 no.3
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• pp.193-198
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• 2017

Sialylation is important in producing therapeutic proteins such as antibody, cytokine and fusion protein. Thus, enhancement of sialylation is usually performed in CHO cell cultures. ${\alpha}2,6$-Sialyltransferase (ST), which plays a key role in the attachment of ${\alpha}2,6-sialic$ acid, is present in human cells but not in Chinese hamster ovary (CHO) cells. Overexpression of ${\alpha}2,6-ST$ can be used for enhancing the degree of sialylation and achieving human-like glycosylation. In this study, we constructed CHO cells producing human cytotoxic T-lymphocyte antigen4-immunoglobulin (hCTLA4-Ig) as well as ${\alpha}2,6-ST$. Transfected CHO cells were selected using G418 and stable cell line was established. Profiles of viable cell density and hCTLA4-Ig titer in an overexpressed cell line were similar to those of a wild-type cell line. It was confirmed that the total amount of sialic acid was increased and ${\alpha}2,6-sialic$ acid was attached to the terminal residues of N-glycan of hCTLA4-Ig by ESI-LC-MS. Compared to 100% of ${\alpha}2,3-sialic$ acid in wild type cells, 70.9% of total sialylated N-glycans were composed of ${\alpha}2,6-sialic$ acid in transfected cells. In conclusion, overexpression of ${\alpha}2,6-ST$ in CHO cells led to the increase of both the amount of total sialylated N-glycan and the content of ${\alpha}2,6-sialic$ acid, which is more resemble to human-like structure of glycosylation.

• The Journal of the Korea Contents Association
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• v.12 no.12
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• pp.304-312
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• 2012

In this study, for behavior clinical signs and blood tests when autumn sudden fevered disease, Tsutsugamushi Disease is Single infected with mixing hemorrhagic fever with renal syndrom.221 positive patients, who have antibody titer more than 1:80 in serologic examination of Tsutsugamushi Disease in a general hospital, Jeollanam-do province, were adopted as a Analysis. in respect of frequency. Single infection patients of Tsutsugamushi Disease were 183 and mixed infection patients with Hemorrhagic Fever with Renal Syndrome were 38. In respect of age, patients over the 70's were mostly infected. In respect of sex, male patients were 70(31.7%) and female patients were 151(66.8%) Women were more infected than men. In respect of outbreak time, the more than 70% disease occurrence of all was charged in October and November and it also occurred in spring. In the clinical signs, fever(p=0.028), urtication(p=0.000) and muscle pain(p=0.000) of mixed infection was more higher than single infection. And in the blood tests, AST(p=0.000), ALT(p=0.000), blood urea nitrogen(p=0.002), total bilirubin(p=0.000) value of mixed infection was more higher than single infection.