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Effects of immersion vaccination in different concentration of edwardsiellosis vaccine on olive flounder, Paralichthys olivaceus  

Gwon, Mun-Gyeong (국립수산과학원)
Bang, Jong-Deuk (국립수산과학원)
Publication Information
Journal of fish pathology / v.17, no.3, 2004 , pp. 171-177 More about this Journal
Abstract
This study was performed to investigate the effects of prolonged immersion vaccination in a dilute vaccine on the farmed olive flounder, Pararlichthys olivaceus. Juvenile olive flounders were immersed in the several diluted ($10^0$, $10^{-1}$, $10^{-2}$ and $10^{-3}$) commercial edwardsiellosis bacterins (5㎎/ℓ) for 2min (short immersion, SI) and 24 h (prolonged immersion, PI). After immunization, fish were analyzed to investigate on non-specific (serum lysozyme and bactericidal activity), specific immune responses (antibody agglutinin titer) and resistances against Edwardsiella tarda infection. The PI of the commercial vaccine diluted with $10^{-1}$ and $10^{-2}$ showed better results over the untreated control in terms of serum lysozyme, bactericidal activities and resistance against E. tarda infection. These results suggest that the prolonged treatments of the diluted vaccine might be a new approach improving the vaccination potential.
Keywords
Edwardsiella tarda, Vaccine, Immune response, Immersion, Olive flounder
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