Browse > Article

Development of DNA Vaccine Against Red Sea Bream Iridovirus (RSIV)  

PARK SO-JIN (Department of Biotechnology and Bioengineering, Pukyong National University)
SEO HYO-JIN (Interdisciplinary Program of Marine Biotechnology, Pukyong National University)
SON JEONG HWA (Department of Biotechnology and Bioengineering, Pukyong National University)
KIM HYOUNG-JUN (Department of Aquatic Life Medicine, Pukyong National University)
KIM YUN-IM (Department of Biotechnology and Bioengineering, Pukyong National University)
KIM KI-HONG (Department of Aquatic Life Medicine, Pukyong National University)
NAM YOON-KWON (Department of Aquaculture, Pukyong National University)
KIM SUNG-KOO (Interdisciplinary Program of Marine Biotechnology, Pukyong National University)
Publication Information
Journal of Microbiology and Biotechnology / v.15, no.4, 2005 , pp. 873-879 More about this Journal
Abstract
Red sea bream iridovirus (RSIV) obtained from infected rock bream was propagated by Bluegill fry-2 (BF-2) cell culture. The virus titer was determined as $10^{5.5}\;TCID_{50}/ml$ on confluent BF-2 cell monolayers. The integrin binding site of ORF 055L of infectious spleen and kidney necrosis virus (ISKNV) was selected for the construction of a primer to obtain the RSIV ORF 055L gene. The genes were amplified using RSIV gene lyzate by PCR. The homologies of the ORF 055L sequence of RSIV with ISKNV and rock bream iridovirus (RBIV) were approximately $96\%$ and $100\%$, respectively. DNA vaccine was constructed by cloning the ORF 055L of RSN into pcDNA 3.1 (+), containing a cytomegalovirus (CMV) promoter. For antibody production, pcDNA-055 DNA vaccine was injected to BALB/c mice. The production of antibodies against pcDNA-055 DNA vaccine was confirmed by the Western blot analysis. The antibodies produced by the pcDNA-055 DNA vaccine showed efficacy to neutralize the RSIV in the neutralization test in BF-2 cell culture.
Keywords
Red sea bream iridovirus (RSIV); BF-2 cells; DNA vaccine; integrin binding site; pcDNA-055 DNA vaccine;
Citations & Related Records

Times Cited By Web Of Science : 7  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Bergmann-Leitner, E. S. and W. W. Leitner. 2004. Danger, death and DNA vaccines. Microbes Infect. 6: 319-327   DOI   ScienceOn
2 Do, J. W., C. H. Moon, H. J. Kim, M. S. Ko, S. B. Kim, J. H. Son, J. S. Kim, E. J. An, M. K. Kim, S. K. Lee, M. S. Han, S. J. Cha, M. S. Park, M. A. Park, Y. C. Kim, J. W. Kim, and J. W. Park. 2004. Complete genomic DNA sequence of rock bream iridovirus. Virology 325: 351-363   DOI   ScienceOn
3 He, J. G., M. Deng, S. P. Weng, Z. Li, S. Y. Zhou, Q. X. Long, X. Z. Wang, and S. M. Chant. 2001 Complete genome analysis of the Mandarin fish infectious spleen and kidney necrosis iridovirus. Virology 291: 126-139   DOI   ScienceOn
4 Mahy, B. W. J. and H. O. Kangro. 1996. Virology Methods Manual. Academic Press, New York, U.S.A
5 Nakajima, K. and M. Sorimachi. 1994. Biological and physico-chemical properties of the iridovirus isolated from cultured red sea bream, Pagrus major. Fish Pathol. 29: 29-33   DOI
6 Nakajima, K. and M. Sorimachi. 1995. Production of monoclonal antibodies against red sea bream iridovirus. Fish Pathol. 30: 47-52   DOI
7 Shi, C., Q. Wei, K. Y. H. Gin, and T. J. Lam. 2003. Production and characterization of monoclonal antibodies to a grouper iridovirus. J. Virol. Methods 107: 147-154   DOI   ScienceOn
8 Van Regennortel, M. H. V., C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle, and E. B. Wickner. 2000. Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press, New York, U.S.A
9 Seo, J. Y. 2003. Protective immunogenicity of the 0 protein of hirame rhabdovirus (HIRRV) in flounder using DNA vaccine. Master Thesis, Pukyong Natl. Univ., Busan, Korea
10 Johansson, S., O. Svineng, K. Wennerberg, A. Armulik, and L. Lohikangas. 1997. Fibronectin-integrin interactions. Front. Biosci. 2: 126-146   DOI
11 Lai, Y. S., H. C. Chic, S. Murali, J. C. Ouo, S. C. Chen, K. Fang, and C. Y. Chang. 2001. In vitro neutralization by monoclonal antibodies against yellow grouper nervous necrosis virus (YONNV) and immunolocalization of virus infection in yellow grouper, Epinephelus awoara (Temminck & Schlegel). J. Fish Dis. 24: 237-244   DOI   ScienceOn
12 Villen, J., E. de Oliveira, J. J. Nunez, N. Molina, F. Sobrino, and D. Andreu. 2004. Towards a multi-site synthetic vaccine to foot-and-mouth disease: Addition of discontinuous site peptide mimic increases the neutralization response in immunized animals. Vaccine 22: 3523-3529   DOI   ScienceOn
13 Yoo, T. H., H. S. Kim, S. S. Park, E. Y. Bang, Y. K. Oh, L. S. Kim, H. Kim, B. K. Hur, Y. W. Ryu, and J. S. Kim. 2003. Notes: Measurement of free polysaccharide in Tetanus Toxoid-conjugate vaccine using antibody/ammonium sulfate precipitation. J. Microbiol. Biotechnol. 13: 469-472
14 Bruce, M. P., V. Boyd, C. Ouch, and J. R. White. 2002. Dialysis-based bioreactor systems for the production of monoclonal antibodies - alternatives to ascites production in mice. J. Immunol. Methods 264: 59-68   DOI   PUBMED   ScienceOn
15 Jeong, J. B. 2003. Comparison of red sea bream iridoviruses by the characterization of DNA nucleotide sequences. Master Thesis, Pukyong Natl. Univ., Busan, Korea
16 Qoi, L. S. M., H. Wang, C. W. Luk, and V. E. C. Ooi. 2004. Anticancer and antiviral activities of Youngia japonica (L.) DC (Asteraceae, Compositae). J. Ethnopharmacol. 94: 117-122   DOI   ScienceOn
17 Shiokawa, S., Y. Yoshimura, H. Sawa, S. Nagamatsu, H. Hanashi, K. Sakai, M. Ando, and Y. Nakamura. 1999. Functional role of arg-gly-asp (RGD)-binding sites on pi integrin in embryo implantation using mouse blastocysts and human decidua. Biol. Reprod. 60: 1468-1474   DOI   ScienceOn
18 Yong, P. J., H. Kim, H. K. Hwang, S. J. Lee, H. S. Kim, B. K. Hur, Y. B. Ryu, C. N. An, and J. S. Kim. 2004. Largescale production of rotavirus VLP as vaccine candidate using baculovirus expression vector system (BEYS). J. Microbiol. Biotechnol. 14: 35-40
19 Bae, C. S., G Y. Lim, J. S. Kim, and B. K. Hur. 2003. Quadrivalent combined vaccine, including Diphtheria Toxoid, Tetanus Toxoid, detoxified whole cell pertussis, and hepatitis B surface antigen. J. Microbiol. Biotechnol. 13: 338-343
20 Gibson-Kueh, S., P. Netto, O. H. Ngoh-Lim, S. F. Chang, L. L. Ho, Q. W. Qin, F. H. C. Chua, M. L. Ng, and H. W. Ferguson. 2003. The pathology of systemic iridoviral disease in fish. J. Camp. Pathol. 129: 111-119   DOI   ScienceOn
21 Kim, J. O., J. H. Shin, S. Y. Baek, K. I. Min, B. S. Min, S. R. Ryu, B. G Kim, D. K. Kim, M. J. Ahn, M. K. Park, H. W. Song, C. K. Lee, and S. H. Lee. 2004. A collaborative study on Korean standard JE vaccine for potency assay. J. Microbiol. Biotechnol. 14: 745- 750
22 Nakajima, K., Y. Maeno, K. Yokoyama, C. Kaji, and S. Manabe. 1998. Antigen analysis of red sea bream iridovirus and comparison with other fish iridoviruses. Fish Pathol. 33: 73-78   DOI
23 Robinson, H. L. and C. A. T. Torres. 1997. DNA vaccines. Semin. Immunol. 9: 271-283   DOI   ScienceOn
24 Blystone, S. D. 2004. Integrating an integrin: A direct route to actin. Biochim. Biophys. Acta 1692: 47-54   DOI   ScienceOn
25 Inouye, K., K. Yamano, Y. Maeno, K. Nakajima, M. Matsuoka, Y. Wada, and M. Sorimachi. 1992. Iridovirus infection of cultured red sea bream, Pagrus major. Fish Pathol. 27: 19-27   DOI
26 Kaufman, R. J., P. C. Brown, and R. T. Schimke. 1981 .'Loss and stabilization of amplified dihydrofolate reductase genes in mouse sarcoma S-180 cell lines. Mol. Cell. Biol. 1: 1084-1093   DOI