• Korean Journal of Veterinary Service
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• v.32 no.4
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• pp.353-359
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• 2009

Interest in marine organisms as potential sources of bioactive agents has increased in recent years. The red seaweed, Callophyllis (C.) japonica, is abundant in the coastal regions of Jeju Island in South Korea. A previous study shows that C. japonica extracts have antioxidant activity and radioprotective effects. In this study, an methanol extract of C. japonica was tested whether it has antibacterial effects against the bacteria from swine. In vitro antibacterial activities of the crude extracts prepared from the C. japonica using 80 % methanol were tested for inhibitory activity against the Escherichia (E.) coli (S175), Enterococcus (E.) faecium (ATCC 51558), Salmonella (S.) Typhimurium and Staphylo-coccus (S.) aureus (ATCC 25923) by using broth dilution method. All organisms were incubated in brain heart infusion medium containing 1% extract at 0, 4, 8, 12 and 24 hrs. The 3 days-old piglets were fed an experimental diet supplemented with 1% C. japonica for 1 week. And the change of the coliform bacteria in feces were examined after supplement of C. japonica for 1 week. When the inocula containing $10^2{\sim}10^3$CFU/ml of each organism were used the extracts of C. japonica showed various degrees of antibacterial effects on all bacteria tested. The CFU value ($6.3\times10^8$CFU/ml) of C. japonica for E. coli was decreased 30% compared with vehicle controls ($9.0\times10^8$CFU/ml) after 8 hrs incubation. The proliferation rate of E. faecium was inhibited about 68% at 4 hrs, 81% at 8 hrs and 76% at 12 hrs after incubation, respectively. The proliferation rate of S. Typhimurium was inhibited about 96% at 4 hrs, 90% at 8 hrs and 72% at 12 hrs after incubation with extracts of C. japonica. The proliferation rate of S. aureus was inhibited more than 90% each time courses. Conclusively, a red seaweed extract of C. japonica was found to be effective against a number of gram negative and gram positive bacteria such as E. coli, E. faecium, S. Typhimurium, and S. aureus. The number of coliform bacteria was increased in the 1% C. japonica-treated group, as compared to those of controls. This result suggests that C. japonica extracts be added as an effective natural antibacterial agent. The precise mechanism of antibacterial effects and its application on swine industry remains to be further studied.

• The Research Journal of the Costume Culture
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• v.17 no.3
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• pp.424-430
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• 2009

Taegwondo uniforms need powerful durability in fabrics against friction and shock because of higher exercising power. Therefore, in this study we developed more comfortable Taegwondo uniform for functional fabrics(deodorization rate, antibacterial activity and static charge). Chitosan is a deacetylated product of chitin, has widely been used in the biomedical sector, food industry, and textile industry. For the purpose of this study, we used the chitosan, which was congenial to the human body. Then, we compared the differences between the chitosan treated fabrics and non treated fabrics of Taegwondo uniform(100% polyester, 65% polyester/35% cotton). Chitosan was dissolved in 1% acetic acid. Also, the fabrics were washed using distilled water. Afterward, its treatment with chitosan was completed by padding the sample to its wet pick-up at $80{\pm}5%$ and by heating $150^{\circ}$ for 3 minutes. The chitosan treated Taegwondo uniform was improved on deodorization rate than non treated uniform. And the chitosan treated Taegwondo uniform was improved remarkably the antibacterial activity in all samples. In the further researches, the static charge was reduced in chitosan treatment uniform.

• Textile Coloration and Finishing
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• v.27 no.1
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• pp.80-95
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• 2015

In this study, the properties such as color, dye uptake and antibacterial function were examined through the application of combination dyeing and mixed dyeing with extracts from Humulus japonicus(hwansam) and the pruned branches of Prunus persica(peach) tree. In terms of dye uptake and surface color changes subject to the kind of mordants, methods and mixing ratio of dye, it was found that fabrics dyed by the pre-mordanting method with $CuSO_4$ showed the red green yellow group colors, which were not seen in fabrics dyed with hwansam only. Color fastness was found strong at grade 4-5 with 96.3% of UV protection ratio. While the fastness to light was as fair as grade 3, it showed an excellent result of 99.9% bacteria reduction rate against Staphylococcus aureus with fair 58% deodorization rate and 21.2% antibacterial rate against Klebsiella pneumoniae. As stated above, the combination and mixed dyeing resulted in the meaningful outcomes with regard to color variations, dye uptake, color fastness and functional properties.

• Textile Coloration and Finishing
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• v.33 no.1
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• pp.24-30
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• 2021

In this study, PET containing 3% silver ion-exchange zeolite was mixed with cotton in a ratio of 6:4 to prepare a spun yarn to evaluate the tensile strength, absorption speed, absorption rate, antibacterial property, and the efficiency of deodorization. As a result, the following conclusions were obtained. First, it can be confirmed that silver ion exchange zeolite is evenly distributed inside and on the surface of the antimicrobial PET-SF through SEM. It was found that the tensile strength between the CVC sample mixed with silver ion zeolite PET and cotton and the normal cotton 100% sample was slightly lower in the CVC sample. Although the absorption speed and water absorption rate were measured to find out the moisture characteristics, it was confirmed that there was no significant difference. The contact angle was slightly larger in the antimicrobial CVC sample, but the time it took for the moisture to completely penetrate into the knit fabric was 0.85 seconds. In addition, it was found that out of the total mixing ratio, 40% of antibacterial PET was spun with regular cotton to produce yarn, which had an excellent bacteria reduction rate of 99.9% and a deodorization efficiency of 85%.

• Journal of the Korea Fashion and Costume Design Association
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• v.24 no.3
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• pp.63-71
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• 2022

This paper aims to compare and verify the antibacterial effects of cotton fabrics naturally dyed with extracts of Ulmus davidiana var. japonica Nakai, Caesalpinia sappan, Saururus chinensis, and Artemisia princeps against antibiotic-resistant strains of bacteria. After natural dyeing of the test fabric, Al, Cu, Fe mordants were used. The color fastness against washing, rubbing, and perspiration of the cotton fabrics dyed with the 4 types of extracts were mostly excellent. However, the color fastness against light showed poor results for all four types. As for the antibacterial test method, MRSA (ATCC 33591) was applied to the cotton fabrics dyed with the four kinds of extracts and cultured for 24 hours. After that, the bacteria that proliferated on the fabrics were collected and spread on a solid medium. The bacteria were measured to find out the bacteriostatic reduction rate for the antibiotic-resistant strains. As a result of the analysis, all four extracts showed a high bacteriostatic reduction rate of more than 99% when the copper mordant was used. Even with the lack of a mordant, the bacteriostatic reduction rate was high, at 99.9% for Caesalpinia sappan and 94.6% for Saururus chinensis.

• Journal of dental hygiene science
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• v.23 no.1
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• pp.51-59
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• 2023

Background: Tea tree oil has antiviral, antimicrobial and antifungal effects and Mastic oil has antifungal and anticancer effects. For synergistic effects of oils, blending oil containing a mixture of two to three oils is recommended. This study aimed to determine the antibacterial effects of Tea tree oil, Mastic oil, and Blending oil containing the two oils in a mixture, to verify and suggest the potential use of these oils as a substance to prevent dental caries. Methods: Tea tree oil, Mastic oil, and Blending oil with a 1:1 blend of the two oils were diluted in liquid medium to 0% (negative control), 0.5%, 1.0%, and 2.0%. Streptococcus mutans was applied to each experimental group of the three diluted oils and after 8 h culture, the optical density (OD) was measured and the growth inhibition rate for S. mutans was estimated. Results: Tea tree oil had significantly low OD values across all concentrations (p<0.05) without significant variation among different concentrations (p>0.05). Mastic oil did not significantly vary in OD compared to the negative control across all concentrations (p>0.05) without significant variation among different concentrations (p>0.05). Blending oil, compared to the negative control, did not significantly vary in OD at 0.5% (p>0.05) but significant variation was found as the concentration increased (p<0.05). Additionally, for Tea tree oil and Mastic oil, the growth inhibition rate showed no significant variation according to concentration (p>0.05), whereas for Blending oil, the growth inhibition rate for S. mutans showed a significant difference at 1.0% (p<0.05) and at higher concentrations. Conclusion: Blending oil containing a Tea tree oil and Mastic oil demonstrated a significant growth inhibition effect on S. mutans from the concentration of 1.0%, which suggested its potential use as an effective antibacterial agent for dental caries.

• KSBB Journal
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• v.19 no.4
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• pp.291-294
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• 2004

The aim of this study was to figure out the antibacterial pattern of leucocin A transformed yeast with culture. Dry cell weight, total secreted protein, and antibacterial activity were increased to 12 hour, after then they showed decrease while protease activity represented the opposite pattern. This implied the production of leucocin A was growth-related. Compared to the result of one hour culture broth, antibacterial activity was about 3.24 fold at 12 hour culture. Maximum growth inhibition rate was 70.57% compared to nontransformed yeast. As the increase of protease in the supernatant, the antibacterial activity was diminished. This study could permit the mass production of bacteriocin to use as antibiotics or food preservatives.

• Journal of the Korea Institute of Building Construction
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• v.23 no.4
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• pp.393-404
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• 2023

This research evaluates the antibacterial and transparency properties of UV-curable paint augmented with 20wt% copper particles. The transparency assessment indicated that the paint maintained a total luminous transmittance of 90% or above, thereby matching or surpassing the performance of standard UV-curing paints. To further test its antibacterial capabilities, wooden flooring boards were coated with the UV-curable paint laced with 20wt% copper particles, which had been surface-modified with a silane coupling agent. Following the fatigue tests of these treated boards, an impressive bacterial reduction rate of 99.9% was noted after a span of 6 hours, demonstrating the paint's exceptional antibacterial performance.

• Textile Coloration and Finishing
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• v.16 no.2
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• pp.25-33
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• 2004

Melange yams were prepared by varing the ratio of raw cotton dyed with Indigo, Gardenia and Sappan wood colorants, respectively. The antibacterial activity and deodorization on melange yarns were evaluated for bacteria reduction rate and deodorization rate. The bacterial reduction rate on melange yarns was explored with Staphylococcus aureus, Klebsiella pneumoniae. The melange yarns on the ratio of raw cotton dyed with Sappan wood and Indigo showed high Staphylococcus aureus reduction rate of 99% and 90%, regardless of Al-mordanting treatment, but those with Gadenia hardly showed bacteria reduction rate. The melange yarn showed high Klebsiella pneumoniae reduction rate of 99% for Al-mordanting treatment with Sappan wood. The others were poor. The deodorization of 81% and 98∼99% obtained with melange raw sliver and melange sliver dyed with Indigo, Gardenia and Sappan wood colorants, but dyeing did not significantly affect deodorization rate.

• Journal of The Korean Society of Integrative Medicine
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• v.11 no.2
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• pp.149-157
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• 2023

Purpose : In this study, to prove the antibacterial effect of Disocorea batatas, which is widely used for food, and to confirm the growth inhibitory effect, the antibacterial activity against L. gasseri, S. mutans, and P. gingivalis was verified. Based on this, it is intended to verify the utility as a preventive and therapeutic composition for dental caries and periodontal disease. Methods : RAW 264.7 cells were used to verify the cell survival rate and NO (Nitric Oxide) inhibitory effect on Disocorea batatas ethanol extract (DBEE). In order to verify the antibacterial effect against L. gasseri, S. mutans, and P. gingivalis, concentrations of 125, 250, and 500 ㎎/㎖ of DBEE were used and measured by the disk diffusion method. In order to confirm the growth inhibitory effect, the absorbance was measured at 600 ㎚ at 3, 6, 12, 18, and 24 hours using the liquid medium dilution method, and the growth inhibitory effect was measured compared to the control group. Results : The cell viability for DBEE was 91 % at 50 ㎎/㎖, and there was no cytotoxicity. The NO production inhibitory effect was shown from 10 ㎍/ml, and the higher the concentration, the greater the inhibitory effect. As for the antimicrobial effect using the disk diffusion method, the higher the concentration, the higher the antibacterial effect. At 125 ㎎/㎖ and 250 ㎎/㎖, S. mutans and L. gasseri showed high antimicrobial activity, and at 500 ㎎/㎖, the antibacterial effect was higher in L. gasseri. The growth inhibitory effect in DBEE was concentration-dependent as the higher the concentration, the higher the growth inhibitory effect, and all of them began to show growth inhibitory effects from 6 hours. Conclusion : Considering that it is widely used as an edible and medicinal material, Disocorea batatas has shown the potential to be used as a substance to prevent and alleviate dental caries and periodontal diseases, and it is believed that further research can be applied to oral health care products.