• Advances in Traditional Medicine
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• 제9권1호
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• pp.90-96
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• 2009

The antibacterial activity-guided fractionation of the MeOH extract of seeds of traditional medicinal plant Prunus japonica resulted in the isolation of linoleic acid and cis-11-eicosenoic acids, and their methyl esters. Linoleic acid inhibited the growth of Propionibacterium acnes, the acne-causing anaerobic bacterium, but cis-11-eicosenoic acid, methyl linoleate, and cis-11-eicosenoate were found to be inactive. Together with isolated linoleic acid, authentic saturated and unsaturated fatty acids were also tested against P. acnes with other bacteria and fungi. Most of the unsaturated fatty acids possessed anti-acne (MIC $16-128{\mu}g/mL$) and antimicrobial properties.

• Mycobiology
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• 제36권2호
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• pp.93-98
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• 2008

Two Schiff base ligands $L_1\;and\;L_2$ were obtained by the condensation of glycylglycine respectively with imidazole-2-carboxaldehyde and indole-3-carboxaldehyde and their complexes with Zn(II) were prepared and characterized by microanalytical, conductivity measurement, IR, UV-Vis., XRD and SEM. The molar conductance measurement indicates that the Zn(II) complexes are 1:1 electrolytes. The IR data demonstrate the tetradentate binding of $L_1$ and tridentate binding of $L_2$. The XRD data show that Zn(II) complexes with $L_1\;and\;L_2$ have the crystallite sizes of 53 and 61 nm respectively. The surface morphology of the complexes was studied using SEM. The in vitro biological screening effects of the investigated compounds were tested against the bacterial species Staphylococcus aureus, Escherichia coli, Klebsiella pneumaniae, Proteus vulgaris and Pseudomonas aeruginosa and fungal species Aspergillus niger, Rhizopus stolonifer, Aspergillus flavus, Rhizoctonia bataicola and Candida albicans by the disc diffusion method. A comparative study of inhibition values of the Schiff base ligands and their complexes indicates that the complexes exhibit higher antimicrobial activity than the free ligands. Zinc ions are proven to be essential for the growth-inhibitor effect. The extent of inhibition appeared to be strongly dependent on the initial cell density and on the growth medium.

• Journal of Microbiology and Biotechnology
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• 제18권5호
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• pp.990-996
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• 2008

In a previous study, we found an antifungal effect on human pathogenic fungi by the cell-penetrating peptide Tat (47-58) derived from HIV-1. Tat (47-58) immediately entered into the fungal nucleus and affected some physiological changes on the intracellular condition. In this study, Tat (47-58) showed a broad spectrum of antibacterial activity against pathogenic bacteria including bacterial clinical isolates. To improve resistance against proteases for use in vivo, we synthesized an analog of Tat (47-58) by substituting the L-amino acid for the D-amino acid. The D-enantiomer of Tat (47-58) also exhibited a broad spectrum of antibacterial activity at almost the same level of L-Tat (47-58) concentration. Unlike L-Tat (47-58), D-Tat (47-58) showed a significant proteolytic resistance against all proteases tested and antimicrobial activities in the presence of trypsin. Moreover, D-Tat (47-58) inhibited MRSA infection in human HeLa cells whereas L-Tat (47-58) partially allowed MRSA infection, and the results were due to the proteolytic resistance of D-Tat (47-58).

• 펄프종이기술
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• 제33권3호
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• pp.69-74
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• 2001

Diaper rash is found on the skin inside of babys diaper area. Most diaper rashes are caused by prolonged contact with the moisture, germs, and ammonia of the stool and urine. Some diaper rashes are caused by fungi infection such as candida albicans and Preteus vulgaris. In this study, Candida albicans was used as a test microorganism and experiment was carried out to inhibit diaper rash. Handsheets were treated with chitosan oligosaccharide, bamboo extractives, as well as mixture of monolaurin as an emulsifier and bamboo extractives. Both shake flask method and halo test were applied in order to examine the antimicrobial activity of each sample. It was found from both results of the halo test and shake flask method that handsheets treated with chitisan oligosaccharide or bamboo extractives showed antimicrobial property. In the treatment of bamboo extractives without emulsifier, no distinct difference in antimicrobial effect was found between bamboo branch and bamboo leaves extractives. In case of mixture of bamboo extractives and emulsifier, the result also suggested that bamboo branch showed better syngergistic effect than bamboo leaves. Both chitosan oligosaccharide and bamboo extractive can be used as antifungal agents against Candida albicans for making diaper.

• 디지털융복합연구
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• 제18권4호
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• pp.309-314
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• 2020

Polymethyl methacrylate (PMMA)는 치과 교정장치 재료로 사용해 왔다. 하지만 낮은 항균력으로 인한 구강감염을 촉진시킬 염려가 있다. 본 연구에서는 이를 극복하기 위하여 최근 발표된 Ag-MSN, graphene-oxide nanosheets(nGO), Cerium nanoparticle(CNP)의 나노입자를 교정레진인 Dentarum 회사의 Orthocryl를 사용하여 레진 파우더 대비 중량비 0, 0.25, 0.5, 1.0, 2.0%를 MMA 액에 혼합한 후 제조사의 지시에 따라 PMMA 분말: MMA 액 (1.2 : 1) 비율로 혼합하여 교정용 시편을 제작하였고 교정용 레진 항진균 실험, 통계분석을 하였다. 항균실험을 한 결과 교정용 PMMA레진이 Ag-MSN을 함유할 때 항(진)균적 성질이 증가하였다. nGO를 PM에 넣으면 표면이 거칠어지고 친수성을 높일 수 있었고 항균 접착제 효과가 표면 거칠기의 변화가 아닌 친수성이 증가하였다. CNP 농도가 높을수록 항부착 성질이 최대 60%까지 관찰되었다. 나노입자함유 교정용 레진은 항균효과가 나타남을 알 수 있었다. 이는 의치, 치열 교정 장치 및 임시 수복물에 대한 유망한 항균 치과 재료로서의 잠재적 유용성을 시사한다.

• Applied Biological Chemistry
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• 제51권3호
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• pp.183-187
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• 2008

본 연구에서는 마늘 중의 항미생물 작용을 가지고 있는 allyl-2-propenyl-1-thiosulfinate의 유사체들인 alkyl thiosulfinate 및 이의 산화물인 thiosulfonate화합물들을 합성하고 이들의 생물 활성을 검사하였다. Alkylsulfinate는 이황화 화합물(disulfide)를 유기 과산화산으로, 또 alkyl thiosulfonate는 thiosulfinite를 sodium periodate로 산화시켜 합성하였다. 합성한 모든 alkylthiosulfinate 및 alkyl thiosulfonate들은 Staphylococcus aureus B33에 대해서는 항세균성을, Candida utilis ATCC42416에 대해서는 항곰팡이성을 나타내었다. 나아가 이들 화합물들은 항산화성과 항응고 활성도 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제10권3호
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• pp.394-398
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• 2000

A methanol extract of the root of Peucedanum japonicum, used as a medicinal herb, showed an inhibitory effect on mammalian topoisomerase I activity. The methanol extract was suspended in ethyl acetate, and a topoisomerase I inhibitor in the organic soluble fraction was then isolated by silica gel and thin layer chromatography. The topoisomerase I inhibitory compound was indentified as falcarindiol based on the analysis of EI-MS, $^1$H and \ulcornerC NMR spectroscopy. This inhibitory showed cytotoxicity against human leukemia Jurkat T and HL60 cells with an IC\ulcorner value of 7 $\mu\textrm{g}$/ml. These results suggest the possibility of falcarindiol as a new anticancer agent which can be expected to have a synergistic effect on other anticancer drugs. In addition, the present data show that falcarindiol has antifungal, yet not antibacterial, activity.

• 한국식품과학회지
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• 제37권2호
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• pp.206-213
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• 2005

본 연구에서는 32종 생약재로부터 70% 에탄올과 물 추출물을 제조하고, 이들의 항균, 항곰팡이, 항암, 항고혈압활성을 조사하였다. 생약재외 추출물 제조는 32종의 생약재를 100에서 2시간동안 추출한 후 동결 건조하였다. 항균활성은 6종의 병원성 미생물을 사용하여 32종 생약재의 물 추출물과 70% 에탄올추출물에 대해 조사하였다. 그중 가자의 물 추출물과 70% 에탄올 추출물을 $125{\mu}g$ 첨가했을 때 14mm 이상의 항균 억제환을 나타내어 가장 좋은 항균활성을 보였다. 항곰팡이 활성은 5종의 곰팡이를 사용하여 32종 생약재의 물 추출물과 70% 에탄올추출물에 대해 paper disc확산법으로 조사하였다. 그 결과 계피의 70% 에탄올추출물을 $1000{\mu}g$ 첨가했을 때 10mm이상의 항균 억제환을 보여 가장 좋은 항곰팡이 활성을 나타내었다. 32종의 생약재 물 추출물과 70% 에탄을 추출물에 대한 ACE저해 활성을 조사하였다. 그 결과 파고지의 물 추출물이 10mf/mL의 농도에서 65.2%의 억제율을 나타내었으며, 파고지의 물 추출물로부터 sephacryl S-300 컬럼으로 분획한 Fr I의 분획물이 5mg/mL의 농도에서 81.1%의 억제율을 나타내었다. 생약재의 물 추출물과 70%에탄올 추출물의 세포독성을 조사하였다. 선정된 6종의 생약재중 정향의 에탄올 침전분획이 암세포에 대해 가장 우수한 세포독성을 나타내었다.

• Mycobiology
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• 제35권4호
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• pp.210-214
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• 2007

Antibacterial and antifungal activities of liquid culture filtrate, water and ethanol extract (solid culture) of Stereum ostrea were evaluated against 5 bacteria and 3 plant pathogenic fungi. To determine the minimal inhibitory concentration (MIC), we studied $5{\sim}300\;mg/ml$ concentrations against bacteria and fungi separately. The MIC was 10 mg/ml for Bacillus subtilis and 40 mg/ml for Colletotrichum gloeosporioides and Colletotrichum miyabeanus. Liquid culture filtrate was more effective against Gram positive than Gram negative bacteria, and Staphylococcus aureus was the most inhibited (20.3 mm) bacterium. Water and ethanol extracts were effective against both Gram positive and Gram negative bacteria, and water extract was better than ethanol extract. In water and ethanol extract, inhibition zones were 23.6 and 21.0 mm (S. aureus) and 26.3 and 22.3 mm (Pseudomonas aeruginosa), respectively. For plant pathogenic fungi, the highest and lowest percent inhibition of mycelial growth (PIMG) was found 82.8 and 14.4 against C. miyabeanus and Botrytis cinerea in liquid culture filtrate, respectively. In water extract, the PIMG was found to be the highest 85.2 and lowest 41.7 for C. miyabeanus and C. gloeosporioides, respectively. The inhibitory effect of ethanol extract was better against C. miyabeanus than C. gloeosporioides and B. cinerea. Among 3 samples, water extract was the best against tested pathogenic fungi. This study offers that the extracts isolated from S. ostrea contain potential compounds which inhibit the growth of both bacteria and fungi.

• 대한화학회지
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• 제65권2호
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• pp.93-105
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• 2021

Mononuclear Cu(II), Ni(II), Co(II), Mn(II), Zn(II), Fe(III), Ru(III), and UO2(II) complexes of 2-hydroxy-4-(p-tolyldiazenyl)benzylidene)-2-(p-tolylamino)acetohydrazide (H2L) were prepared by direct method. The ligand and its complexes were isolated in solid state and characterized by analytical techniques such as elemental and thermal analyses, molar conductance, magnetic susceptibility measurements and spectroscopic techniques such as UV-Visible, IR, 1H-NMR and 13C-NMR. The spectral data indicated that the ligand acted as neutral/monobasic bidentate or monobasic/dibasic tridentate ligand bonded to the metal ions through the oxygen atom of ketonic or enolic carbonyl group, azomethine nitrogen atom and deprotonated/protonated phenolic oxygen atom forming either tetragonally distorted octahedral or octahedral. Antimicrobial activities of the ligand and its complexes were evaluated against Escherichia coli, Bacillus subtilis and Aspergillus niger by well diffusion method. The results of antifungal activity showed that the Fe(III) complex (10) exhibited higher antifungal against Aspergillus niger than the other complexes. However, the results of antibacterial activity revealed that Cu(II) complex (4) is the most active against Escherichia coli while the Cu(II) complex (5) and Fe(III) complex (10) exhibited higher antibacterial effect on Bacillus subtilis than the other complexes.