• YAKHAK HOEJI
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• v.42 no.1
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• pp.89-94
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• 1998

Propionibacterium acnes is the pharmacological target site of antiacne drugs. We have examined the antiacne activity of ninety seven natural products which have been used as Korean traditional medicines in various skin disorders. The antibacterial activity of extracts from the natural products were evaluated against P. acnes ATCC 9616 by disc method. Twelve natural products showed the potent antibacterial activity against P. acnes, and were, selected for the minimal inhibitory concentration(MC) against P. acnes. MICs of nine extracts were below 0.3% (w/v) and Sophora flavescens showed the most potent activity with a MIC of less than 0.008%(w/v) against P. acnes. Thus, the results suggest that nine natural products including S. flavescens can be developed as sources of promising potent antiacne agents.

• Archives of Pharmacal Research
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• v.17 no.2
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• pp.131-133
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• 1994

New istain-3-isonicotinylhydrazones, isationazine and its Mannich bases and spiro (indoline-3,2'-thiadiazoline)-2-one have been synthesized. These compounds have been screenced for their antibacterial activity against the Gram-positive and Gram-negative bacteria.

• Korean journal of applied entomology
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• v.62 no.2
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• pp.95-102
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• 2023

The number of households with pets has increased in recent years. Consequently, interest in mites and their odors has increased. In this study, we investigated whether pyrethrum extract and water-soluble phytoncides are effective tick repellents, deodorizing agents, and antibacterial agents. In the tick-climbing test, the pyrethrum extract (23.33 points) and water-soluble phytoncide (22.00 points) showed a high repelling effect. To confirm the tick avoidance effect of the composite extract, a tick climbing test was conducted with a pest control extract, a composite extract (referred to as a sample) containing a water-soluble phytoncide, and a third-party product (control). The sample showed a high avoidance effect (13.00 points and Control 26.67 points. An indoor repellent efficacy test for ticks confirmed that the sample showed an excellent tick-repellent effect (88%) compared to the control (12%). As a result of a deodorization test, ammonia was shown to have a deodorizing effect of 98.3% and trimethylamine of 99.5%, whereas, in the antibacterial test, the E. coli, Staphylococcus aureus, and pneumonia groups showed an antibacterial effect of 99.9%. Combining the results of this study, it is judged that the complex extract including the above material has high utility value as a material that is effective in mite repellents, deodorization, and antibacterial activity.

• Journal of Food Hygiene and Safety
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• v.8 no.1
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• pp.17-23
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• 1993

In an attempt to quantitate and qualitate residual antibiotics and antibacterial agents n meat simultaneously, we studied a gas chromatogrphy-mass spectrometry (GC/MS) analysis. For a simultaneous analysis of macrolide antibiotics such as erythromycin and tylosin in meat, the homogenization with MeOH, defatting with n-hexane, extraction with CHCl3, elution with CHCl3 : MeOH=2:1 from Sep-Pak silica cartridge, acid gydrolysis, back extraction with CHCl3, and quantitation by selected ion monitoring(SIM) mode after trimethylsilyl derivatization were performed. The recoveries of erythromycin and tylosin (CV,%) at 10 ppm fortification level were 90.59(4.89) and 45.91(0.20) , and the detection limits of those were 0.02 and 2.0 $\mu\textrm{g}$/g beef, respectively. From these results, the developed analytical method using GC/MS-SIM mode allows excellent detection and quantitation of residual macrolide antibiotics in meats, using complementary method with bio-assay.

• KCI Concrete Journal
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• v.14 no.4
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• pp.171-177
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• 2002

Antifungal agents are used to impart antibacterial or bactericidal properties to commodities and various articles used in industries and can be classified into two broad groups i.e organic and inorganic. Inorganic antifungal agents comprise of Ag, Zn, or Cu, etc. These elements tend to exhibit high level of antifungal activities, non-uniform dispersion in substrates, and have poor properties in expensive and cheap adhesiveness. In this study, the organic antifungal agent was used for the purpose of investigating the antifungal activity of antifungal agent activated-cement mortar (AACM) on the aspergilus niger of various fungus which can be easily discovered in the interiors and exteriors of buildings. In addition, an experiment on the basic physical properties of AACM such as compressive and flexural strength was carried out. The conclusion of this investigation revealed that a dosage increase of antifungal agent exhibits a high inhibitory effect on the aspergilus niger, and although there is a slight decrease in the strength of AACM, the strength of AACM was almost equal to that of inactivated cement mortar.

• Journal of the Korean Chemical Society
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• v.65 no.5
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• pp.320-326
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• 2021

This study investigated the effect of the structure of the crosslinking agent used in contact lens polymerization on the physical properties and drug dissolution of contact lenses.es Contact lenses were manufactured using 0.3% and 3% of 4 types of crosslinking agents, respectively, and ofloxacin was used as the drug. Contact lenses using hydrophilic crosslinking agents improved water contents and wettability, and the more hydrophilic functional groups, the greater the effect. Contact lenses with a high concentration of crosslinking agent had a low concentration of eluted drug and a longer release time. The cross-linking agent structure of contact lenses had an effect on improving the performance of contact lenses and controlling drug release.

• Mycobiology
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• v.46 no.4
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• pp.370-381
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• 2018

Bioactive natural compounds, isolated from fungal endophytes, play a promising role in the search for novel drugs. They are an inspiring source for researchers due to their enormous structural diversity and complexity. During the present study fungal endophytes were isolated from a well-known medicinal shrub, Berberis aristata DC. and were explored for their antagonistic and antioxidant potential. B. aristata, an important medicinal shrub with remarkable pharmacological properties, is native to Northern Himalayan region. A total of 131 endophytic fungal isolates belonging to eighteen species and nine genera were obtained from three hundred and thirty surface sterilized segments of different tissues of B. aristata. The isolated fungi were classified on the basis of morphological and molecular analysis. Diversity and species richness was found to be higher in leaf tissues as compared to root and stem. Antibacterial activity demonstrated that the crude ethyl acetate extract of 80% isolates exhibited significant results against one or more bacterial pathogens. Ethyl acetate extract of Alternaria macrospora was found to have potential antibacterial activity. Significant antioxidant activity was also found in crude ethyl acetate extracts of Alternaria alternata and Aspergillus flavus. Similarly, antagonistic activity of the fungal endophytes revealed that all antagonists possessed inhibition potential against more than one fungal pathogen. This study is an important step towards tapping endophytic fungal diversity for bioactive metabolites which could be a step forward towards development of novel therapeutic agents.

• The Plant Pathology Journal
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• v.35 no.5
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• pp.473-485
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• 2019

Kiwifruit (Actinidia spp.) is an economically important crop and a bacterial canker disease, caused by Pseudomonas syringae pv. actinidiae (Psa), is the most destructive disease in kiwifruit production. Therefore, prevent and control of the disease is a critical issue in kiwifruit industry worldwide. Unfortunately, there is no reliable control methods have been developed. Recently, interest in disease control using microbial agents is growing. However, kiwifruit microbiota and their roles in the disease control is mainly remaining unknown. In this study, we secured bacterial libraries from kiwifruit ecospheres (rhizosphere, endospere, and phyllosphere) and screened reliable biocontrol strains against Psa. As the results, Streptomyces racemochromogenes W1SF4, Streptomyces sp. W3SF9 and S. parvulus KPB2 were selected as anti-Psa agents from the libraries. The strains showed forcible antibacterial activity as well as exceptional colonization ability on rhizosphere or phyllosphere of kiwifruit. Genome analyses of the strains suggested that the strains may produce several anti-Psa secondary metabolites. Our results will contribute to develop biocontrol strains against the kiwifruit canker pathogen and the disease management strategies.

• Biodesign
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• v.7 no.2
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• pp.35-37
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• 2019

Xanthomonas oryzae pv. oryzae (Xoo) is a plant pathogen, which causes a bacterial blight of rice. The bacterial blight is one of the most devastating diseases of rice in most of the rice growing countries and there is no effective pesticide against bacterial blight. The β-ketoacyl-acyl carrier protein synthase III (FabH) plays a key role in fatty acid synthesis (FAS) and is a promising drug target for the development of antibacterial agents. Xoo0878 gene, a fabH gene, from Xoo was cloned and its gene product Xoo0878 was expressed, purified and crystallized. Xoo0878 crystal diffracted to 2.1Å resolution and belonged to the triclinic space group P1, with unit-cell parameters a = 57.3Å, b = 64.7Å, c = 104.2Å and α = 81.6°, β = 84.7°, γ = 74.4°. There are four monomers in the asymmetric unit, with a corresponding crystal volume per protein weight of 2.65 ų Da^-1 and a solvent content of 53.6%. Xoo0878 structure will be useful to develop new antibacterial agents against Xoo.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.100-106
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• 2020

Coix lacryma-jobi (Hanjeli) is known to posses anti-microbial properties. Therefore, phytochemical compounds of C. lacryma-jobi have been studied to produce novel antimicrobial agents as treatments against antibiotic-resistant bacteria.The objective of this study was to determine the phytochemical composition and antibacterial activity of the C. lacryma-jobi oil against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. The phytochemical composition of the oil was determined via gas chromatography mass spectrophotometry (GC-MS). Moreover, agar disk and agar well diffusion were employed to screen the antibacterial activity of the oil. An agar well diffusion test was implemented to determinate MIC's (minimum inhibitory concentrations). Dodecanoic acid, tetradecanoic acid, 2,3-dihydroxypropylester, 1,3-dioctanoin, N-methoxy-N-methyl-3,4-dihydro-2H-thiopyran6-carboxamide, propanamide, 5-Amino-1-(quinolin-8-yl)-1,2,3-triazole-4-carboxamide, and pyridine were identified in the C. lacryma-jobi oil. The MIC value of the oil was 0.031 g/L and the MBC of the oil was 0.125 g/L effective in all test bacteria. Dodecanoic acid displayed inhibitory activity against gram-positive and gram-negative bacteria. Therefore, our research demonstrated C. lacryma-jobi (Hanjeli) oil exhibited antibacterial activity against E. coli, S. aureus, and B. subtilis. These research suggest that C. lacryma-jobi root oil could be used for medicinal purposes; however clinical and in vivo tests must be performed to evaluate its potential as an antibacterial agent.