Biodesign

Korean Society for Structural Biology (한국구조생물학회)
권호 표지
DOI QR코드

DOI QR Code

Cloning, expression, purification, and crystallization of Xoo0878, β-ketoacyl-acyl carrier protein synthase III (FabH), from Xanthomonas oryzae pv. oryzae

  • Ngo, Ho-Phuong-Thuy (Department of Biological Sciences, Konkuk University) ;
  • Nguyen, Diem-Quynh (Department of Biological Sciences, Konkuk University) ;
  • Kim, Seunghwan (Genomics Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Kim, Jeong-Gu (Genomics Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Ahn, Yeh-Jin (Department of Biotechnology, Sangmyung University) ;
  • Kang, Lin-Woo (Department of Biological Sciences, Konkuk University)
  • Received : 2019.06.03
  • Accepted : 2019.06.17
  • Published : 2019.06.30
⟨ Previous Next ⟩

Abstract

Xanthomonas oryzae pv. oryzae (Xoo) is a plant pathogen, which causes a bacterial blight of rice. The bacterial blight is one of the most devastating diseases of rice in most of the rice growing countries and there is no effective pesticide against bacterial blight. The β-ketoacyl-acyl carrier protein synthase III (FabH) plays a key role in fatty acid synthesis (FAS) and is a promising drug target for the development of antibacterial agents. Xoo0878 gene, a fabH gene, from Xoo was cloned and its gene product Xoo0878 was expressed, purified and crystallized. Xoo0878 crystal diffracted to 2.1Å resolution and belonged to the triclinic space group P1, with unit-cell parameters a = 57.3Å, b = 64.7Å, c = 104.2Å and α = 81.6°, β = 84.7°, γ = 74.4°. There are four monomers in the asymmetric unit, with a corresponding crystal volume per protein weight of 2.65 Å3 Da-1 and a solvent content of 53.6%. Xoo0878 structure will be useful to develop new antibacterial agents against Xoo.

Keywords

Acknowledgement

We are grateful to the staff members of beamline 5C at the Pohang Accelerator Laboratory (PAL), Republic of Korea. This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ013270012019)" Rural Development Administration, Republic of Korea and the Basic Science Research Program (NRF-2017M3C1B6070572) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning.