• 약학회지
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• 제43권6호
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• pp.756-761
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• 1999

Lipid peroxidation mediated by hydroxyl radicals which are generated during myocardial ischermia has suggested as a possible mechanism of ischemic myocardial damage. Recently, it has been reported that anti-arrhythmic action of lidocaine, a local anesthetic, is attributed to its "membrane-stabilizing" properties through scavenging free radicals, thus, inhibiting lipid peroxidation. Aldehyde oxidase and xanthine oxidase which catalyze the oxidation of many purine, pyrimidine and pteridine derivatives are known as free radical generating systems. In this experiment, we studied the effect of lidocaine and procainamide on the hepatic aldehyde and xanthine oxidase activity and antioxidative activities. It was found that lidocaine and procainamide inhibited both NADPH-dependent and independent lipid peroxidation. Both of tested compounds were found to be ineffective in inhibiting xanthine oxidase. Lidocaine and procainamide, however, inhibited aldehyde oxidase activity in vitro as well as in vivo. Based on the above results, lidocaine and procainamide could be employed as a therapeutic agent for aldehyde oxidaserelated disease.d disease.

• 약학회지
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• 제47권5호
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• pp.307-310
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• 2003

Ethanol extract of Actinidia polygama Max. (AP) was examined for hypouricemic activity in potassium oxonateinduced hyperuricemic animal model. Ethanol extract of AP lowered the plasma uric acid level by 20％ when compared to the hyperuricemic control group. AP ether fraction at a dose of 100 mg/kg showed 27％ reduction, whose efficacy was comparable to the probenecid-treated group. AP ether fraction-treated group also showed 23％ reduction of urate in urine and this result suggests that AP ether fraction has anti-gout activity probably due to xanthine oxidase inhibition.

• 한국미생물·생명공학회지
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• 제40권3호
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• pp.208-214
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• 2012

본 연구는 왕겨를 이용한 항산화, 항노화에 효과적인 초액을 대량생산하여 기능성을 검증하고 왕겨초액 내의 기능성물질을 분석하여 사업화의 가능성을 타진하고자 하였다. 정제된 왕겨초액을 분획하여 기능성이 확인된 부분을 HPLC 기기를 이용하여 확인한 결과 caffeic acid 외에 7종의 polyphenol 유래물질에서 기능성을 확인하였다. 기능성 화장품소재 시험으로는 세포 생존율 측정(MTT assay), 주름개선 (collagenase inhibition assay, elastase inhibition assay), 미백(tyrosinase inhibition assay), 항산화 시험(DPPH free radical scavenging assay, SOD-like activity, xanthine oxidase inhibition assay)이 실시하였다. 피부주름형성은 콜라겐의 합성과 분해가 중요한 원인으로 작용한다고 알려져 있지만, 최근 많은 연구에서 엘라스틴의 재형성과 분해 또한 주름형성 기전에서 중요한 작용을 하는 것으로 보고되고 있다. 왕겨초액은 $100{\mu}L/mL$의 농도에서 collagenase 저해효과가 100%, elastase 저해효과가 55% 이상으로 주름개선 효과가 우수하였다. 왕겨초액은 $100{\mu}L/mL$의 농도에서 tyrosine에 대한 tyrosinase 저해효과가 80% 이상으로 미백효과가 우수하게 나타났다. DPPH radical 소거율, xanthine oxidase 저해와 SOD-like 활성을 이용하여 항산화 효과를 측정하였다. 왕겨초액 $100{\mu}L/mL$의 농도에서 DPPH radical 소거율이 80%, xanthine oxidase 저해효과가 80%, 그리고 SOD-like 활성이 100%를 나타내었다. 따라서 본 연구를 통하여 정제 왕겨초액을 이용한 산업화를 위한 가능성을 타진하여 효과가 좋음을 확인할 수 있었다. 이와 같은 결과로 왕겨초액을 이용한 기능성 화장품의 소재로 뿐만 아니라, 식품 및 의약외품의 품목허가를 통하여 고부가가치의 상품화소재로의 가능성이 있다고 사료됩니다.

• 대한본초학회지
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• 제21권4호
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• pp.103-107
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• 2006

Objectives : This study was investigated to analyze the anti-oxidant activity of water and ethanol extracts from the Magnoliae Flos. Methods : The effects on electron donating ability (EDA), SOD-like activity and xanthine oxidase inhibition have been investigated the anti-oxidant activity for utilization as a natural material of cosmetics. Results : This study was investigated to analyze the anti-oxidant activity of water and ethanol extracts from the Magnoliae Flos. In the electron donating ability test, water and ethanol extract from Magnoliae Flos showed an effect of 75.6% and 83.5% at 1,000 ppm concentration, respectively. In the superoxide dismutase (SOD)-like activity, 1,000 ppm of water extract showed an effect of 45.2% and ethanol extract showed an effect of 64.2%. Xanthine oxidase showed a meaningful inhibitory effect of 68.8% in water extract and 23.2% at 1,000 ppm in ethanol extract. Conclusion : From these results, Magnoliae Flos showed the development possibility as a natural material of anti-oxidant cosmetics.

• 한국균학회지
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• 제43권1호
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• pp.47-52
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• 2015

덕유산 무주 리조트 일대에서 수집한 야생화 27점에서 효모 8종 22균주를 분리 동정하였다. 이들 균주 중에서 Cryptococcus magnus 9균주, Cryptococcus flavescens 6균주와 Cryptococcus sp. 2균주 등 Cryptococcus속 균들이 가장 많이 분리되었다. 이들 22균주들을 YPD 배지에서 24시간 배양하여 각각의 배양 상등액과 무세포 추출물을 제조한 후 이들의 항산화 활성과 항통풍성 xanthine oxidase 저해활성 등의 생리기능성들을 측정한 결과, Sporobolomyces phaffii JS00583과 Rhodotorula graminis JS00581의 배양 상등액이 각각 40.4%와 39.1%의 항통풍성 xanthine oxidase 저해활성을 보였다. 또한 Cryptococcus magnus JS00570의 상등액 역시 36.1%의 미백성 tyrosinase 저해활성을 보였다.

• 한국식품저장유통학회지
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• 제13권5호
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• pp.643-648
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• 2006

본 연구는 뜰보리수 열매의 식품영양학적 평가 및 기능성 물질 발굴의 일환으로 뜰보리수 열매를 이용하여 성숙과정 중의 free radical 소거능을 비롯한 SOD유사활성, 아질산염 소거작용, xanthine oxidase 저해활성 등의 항산화 활성을 조사하였다. 뜰보리수 에탄올 추출물의 전자공여능은 성숙함에 따라 감소하는 경향을 나타내었으며, 에탄올 추출물의 농도가 높을수록 전자공여능의 효과는 더욱 높게 나타났다. 에탄올 추출물의 SOD 유사활성은 성숙정도에 따른 증가하였고, 농도에 비례하였다. 뜰보리수 열매의 에탄을 추출물의 아질산염 소거능은 pH가 낮을수록 높았으며, 농도가 증가할수록 아질산염 소거 작용이 높게 나타났다. Xanthine oxidase 활성 저해능은 완숙과 과숙과실의 추출물에 비해서 미숙과실 추출물에서 높았다.

• 한국약용작물학회지
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• 제22권1호
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• pp.8-16
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• 2014

The present study was carried out to evaluate the antioxidant and anti-inflammatory activities of three extracts (hot water, 50% ethanol and mixed solvent;water, ethanol, butylene glycol, propylene glycol) of dried chestnut, chestnut shell, chestnut leaves and dried chestnut leaves obtained from Castanea crenata tree. When conducted DPPH assay, radical scavenging activity of ethanol extract of chestnut shell was the highest with $IC_{50}$ $10.8{\mu}g/mL$ among four extracts from these parts (p < 0.05). In additional results by the xanthine oxidase assay, antioxidant activity showed that water extract of chestnut leaves showed the highest xanthine oxidase inhibitory activity in the tested extracts (p < 0.05). Futhermore, extracts of chestnut shell and leaves exhibited no cytotoxicity in RAW 264.7 cells (p < 0.05). Also, anti-inflammatory activity by NO assay showed LPS-induced NO was significantly inhibited following treatment with extracts of chestnut shell and leaves of 3mg/mL (p < 0.05). These data suggest that extract of chestnut shell have antioxidant and anti-inflamantory activity including chestnut leaves. Therefore, it is considered that Castanea crenata research range and selection of functional material can broaden chestnut shell to other fractions such as chestnut and chestnut leaves.

• Journal of Acupuncture Research
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• 제40권1호
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• pp.53-60
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• 2023

Background: This study aimed to investigate the effects of Oenanthe javanica (OJ) extracts on rats with potassium oxonate (PO)-induced hyperuricemia. Methods: The effects of OJ extract on rats with PO-induced hyperuricemia-induced were monitored. Changes in the body weight and organ indices of hyperuricemic rats were calculated to detect anti-hyperuricemic effects. Blood samples were collected to observe the effect of reducing serum uric acid concentration. Kidney tissues were stained to observe histopathological changes under a microscope. The activity of xanthine oxidase (XO), which catalyzes xanthine to uric acid in the liver, was assessed to observe the inhibitory effect of XO. Results: 1. The body weight of hyperuricemic rats showed no considerable differences between the control group and the treatment group. The OJ group had significantly improved liver index, whereas the allopurinol group had improved liver and kidney indices. 2. Serum uric acid levels increased significantly after PO injection, and the OJ and allopurinol groups showed a significant reduction effect. 3. PO injection led to the inflammation of kidney tissues, and OJ improved it significantly. 4. The activity of XO after PO injection was significantly increased, and allopurinol significantly inhibited XO activity in the liver. Conclusion: In the hyperuricemia rat model, OJ extract reduced uric acid concentration and demonstrated its anti-inflammatory effects. Thus, OJ extracts can be used to lower uric acid levels.

• 대한한방내과학회지
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• 제23권1호
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• pp.57-64
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• 2002

Objective : This study was designed to investigate the anti-oxidative effects of Oldenlandiae Diffusae Herba Water extract (ODHW) on lipid peroxidation by free radicals oxidative hepatic injury. Methods : In order to evaluate anti-oxidative activities of ODHW in the liver cell, cultured normal rat liver cells(Ac2F) were incubated with or without ODHW. After 16 hours to 18 hours of experiment, cells were placed in DMEM medium without serum, and then incubated with 1mM tert-butyl hydro-peroxide(t-BHP) for two hours. Viable cells were detected by MTT assay. The levels of LPO induced by hydroxyl radical derived from H2O2-Fe2+ system in rat liver homogenate were determined by means of TBA. Inhibitory effect of ODHW on superoxide generation was measured by xanthine-xanthine oxidase system. Results : In the linoleic acid autoxidation system, ODHW exhibited antioxidant activity, which inhibited 85% of linoleic acid peroxidation. These effects were similar to those of dl-a-tocopherol. ODHW showed scavenging effects on DPPH radical, inhibited superoxide generation in xanthine-xanthine oxidase system, and also inhibited lipid peroxidation of rat liver tissue with hydroxyl radical derived from $H_2O_2-Fe^{2+}$ system. In addition, ODHW protected the cell death induced by t-BHP and it significantly increased cell viability in a normal rat liver cell(Ac2F)

• Natural Product Sciences
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• 제8권4호
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• pp.111-126
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• 2002

The chemistry of secondary products from Acanthopanax species and their pharmacological activities were reviewed. A nitrogenous compound, a furan compound, a quinoid, benzoids, coumarins, phenylpropanoids, lignans, flavonoids, terpenoids, phytosterols, polyacetylenes, a pyrimidine, cyclitols, monosaccharides and an aliphatic alcohol have been isolated from Acanthopanax species and have been shown to have various levels of activities such as anti-bacterial, anti-cancer, anti-gout, anti-hepatitis, anti-hyperglycemic, anti-inflammatory, anti-leishmanicidic, anti-oxidant, anti-pyretic, anti-xanthine oxidase, choleretic, hemostatic, hypocholesterolemic, immunostimulatory and radioprotectant effects, etc.