• 한국약용작물학회지
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• 제23권1호
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• pp.37-42
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• 2015

This study was to investigate anti-skin wrinkle effect of Agastache rugosa Kentz extracts by extraction processes. In the comparison of the effect of the solvent extraction, the extracts by 70% ethanol (EE) showed better biological activities that those by hot water. Therefore, further fermented Agastache rugosa was applied to 70% ethanol extraction process (FEE). FEE showed higher DPPH scavenging activity of 62.98% than EE's 62.71% at $1.0mg/m{\ell}$, but there was no significant. Elastase inhibition was measured 23.0% from FEE at $1.0mg/m{\ell}$. Cytotoxicity showed the highest 16.26% from FEE, this value is safe in the cell experiment. Collagen production showed $113.1ng/m{\ell}$ from FEE, on the other hand EE was measured $77.4ng/m{\ell}$ in adding $1.0mg/m{\ell}$. MMP-1 production was observed $1398pg/m{\ell}$ from FEE and EE was measured $1632pg/m{\ell}$. These results were found the highest antioxidant and anti-wrinkle effect. As a result, it was also confirmed that anti-skin wrinkle activities of the Agastache rugosa Kentz extract was correlated with anti-oxidant activities.

• 생명과학회지
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• 제26권7호
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• pp.782-788
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• 2016

본 연구에서는 화장품의 천연소재로 사용 가능함을 알아보기 위해 추출부위가 각각 다른 총 11종의 자생식물의 항산화, 항주름에 관한 생리활성 효능을 스크리닝 하였다. DPPH scavenging assay 측정결과 100 μg/ml 농도에서 70% 넘는 활성을 나타낸 시료는 순비기나무, 송이고랭이, 방울고랭이, 큰고랭이, 자귀풀이었다. Hydrogen peroxide scavenging assay 측정결과 1,000 μg/ml 농도에서 물꼬챙이골을 제외한 10종이 90%넘는 활성을 나타내었다. Xanthine oxidase scavenging assay 측정결과 1,000 μg/ml 농도에서 순비기나무(Leaf; 86.3%)가 가장 높은 활성을 나타냈다. 4가지 항산화 실험에서 모두 효능이 우수하게 나온 시료는 순비기나무, 송이고랭이, 방울고랭이, 큰고랭이, 자귀풀이며 그중 collagenase 저해활성 측정결과 방울고랭이가 가장 높은 저해 활성을 나타내어 항산화 및 항주름에 효능이 있는 시료로 나타났다. 이러한 결과를 바탕으로 활성이 높은 자생식물은 화장품에 사용되는 천연소재로 이용이 가능한 좋은 수종으로 판단된다.

• 동의생리병리학회지
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• 제31권6호
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• pp.380-384
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• 2017

From March 1, 2017 to May 31, 2017, subjects were allowed to apply a wrinkle-improving herbal cosmetic product containing the extract of Douchi for 8 weeks. The effectiveness of herbal cosmetics was evaluated through wrinkle improvement efficacy parameter analysis, subjective wrinkle improvement through questionnaires, and adverse reaction. The herbal cosmetic products containing the extract of Douchi showed a significant decrease in the wrinkle improvement efficacy parameter analysis result. The results of the subjective satisfaction analysis after use were also evaluated positively. We could confirm the possibility of wrinkle improvement effect of Douchi extract, and it will be possible to contribute to the expansion of korean medicine field through more studies in the future.

• 한방안이비인후피부과학회지
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• 제24권2호
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• pp.68-78
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• 2011

Objective : The purpose of this clinical research was to investigate the effects of herbal cosmetics containing Momordica charntia L. extracts on the anti-wrinkle and whitening effects. Methods : A total of 25 subjects who visited Daegu Hanny Oriental Medical Center from November 1st, 2010 to December 31th, 2010 were included in this study. In this study, we observed change of R1, R2, R3, R4, R5 for using products, change of average melanin content and pigmental area, analysis results of subjective wrinkle improvement, Analysis results of subjective whitening improvement. Statistical analysis was performed by using paired t-test and wilcoxon signed ranks test. Statistical significance was achieved if the probability was less than 5%(p<0.05) Results : Statistically, herbal cosmetics containing Momordica charntia L. extracts showed siginificant effect on change of R1, R2, R3, R4, R5 for using products, change of average melanin content and pigmental area, analysis results of subjective wrinkle improvement, Analysis results of subjective whitening improvement(p<0.05). And satisfaction after using herbal cosmetics was near good. Conclusions : Considering the above results, we have concluded that herbal cosmetics containing Momordica charntia L. extracts have the remarkable effects on the anti-wrinkle and whitening effects.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2005년도 춘계학술대회
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• pp.131-148
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• 2005

This study is peformed to investigate the effect of water extract from Vaccinium uliginosum L., on melanin production in B 16 melanoma cells, procollagen production and matrix metalloproteinase-1(MMP-1) inhibition in human fibroblast cells. One hundred grams of the Vaccinium uliginosum L. was extracted with 2000 mL of water($90^{\circ}C$, 16h, 2times). The water extracts were lyophilized and stored at $4^{\circ}C$ until used. Dry weight yields of extracts of Vaccinium uliginosum L. were $3\%$(w/w). Extracts from Vaccinium uliginosum L. showed scavenger activities on DPPH radical, superoxide anion radical, hydroxyl radical, hydrogen peroxide and singlet oxygen radical. And these substances inhibited release of cyiokines from human keratinocyte after UV B exposure. Therefore we confirmed that extracts from Vaccinium uliginosum L. had antioxidative effect. These substances inhibited purified tyrosinase activity and melanogenesis in B 16 melanoma cells treated/untreated IL-$1{\alpha}$. Moreover this extract stimulated procollagen production and inhibited MMP-1 production in human fibroblast cells treated/untreated IL-$1{\beta}$. Therefore we confirmed that extracts from Vaccinium uliginosum L. had whitening effect. And these substances decreased degree of wrinkle in hairless mouse skin that induced by UV B irradiation. Therefore we confirmed that extracts from Vaccinium uliginosum L. had anti-wrinkle effect. From the above results, it is possible that Vaccinium uliginosum L. may be developed to be an anti-melanogenesis agent and anti-wrinkle agent.

• KSBB Journal
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• 제31권3호
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• pp.178-185
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• 2016

Aging of the face is mainly related to the features that are sagging or loss of elasticity of the skin by reducing the volume around the eyes or cheek. Intrinsic aging can be seen to cause thinner dermis, reduction of extracellular matrix and subcutaneous fat. This study was carried out to investigate the skin volume augmentation and anti-wrinkle effects of Tribulus terrestris fruit extract. Skin anti-aging effect of Tribulus terrestris fruit extract was evaluated by using lipid accumulation, expressin of type I procollagen and elastin in preadipocytes and human dermal fibroblasts. Tribulus terrestris fruit extract augmented preadipocytes differentiation about 56% at 100 µg/mL. The type I procollagen and elastin were increased about 35% and 25% by treatment 20% Tribulus terrestris fruit extract, respectively. The clinical study also showed that skin sagging, skin elasticity, and dermal density improved without adverse effect following 4 week application of cream containing 2% Tribulus terrestris fruit extract. We suggest that Tribulus terrestris fruit extract can have the good possibility as skin volume augmenting, skin elasticity and wrinkle improving agent.

• 대한화장품학회지
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• 제30권4호
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• pp.457-462
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• 2004

N-Acetyl-D-glucosamine (NAG)는 보습제로 사용되어지는 히아루론산의 구성물질인 뮤코 다당류의 일종이며, 특히 화장품으로서 응용은 보습제로서의 사용이 최초이다. 본 실험에서는 게나 새우의 껍데기에서 추출된 키틴을 탈아세틸화 하여 얻은 NAG를 화장품 원료로서 적용하고자 하였다. 현재 기능성 주름 원료로 알려진 레티놀(retinol)과 NAG를 비교하기 위하여 섬유아세포의 활성능력 및 콜라겐 생성촉진 효과를 비교 실험하였으며, 제형 내에서의 안정성을 위하여 HPLC로 역가를 측정하였다. 실험결과, 게의 껍질로부터 유도된 NAG는 피부에 자극을 전혀 주지 않으면서 섬유아세포의 세포활성 및 콜라겐의 생성을 촉진시키는 효과를 나타내었으며, 헤어리스 마우스를 대상으로 실험을 실시한 결과 피부층의 변화를 통하여 주름의 감소 효능을 볼 수 있었다.

• Korean Chemical Engineering Research
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• 제48권4호
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• pp.413-416
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• 2010

본 연구에서는 연근의 추출물에서 기능성화장품 소재를 개발하고자 하였다. 용매는 70~100%의 에탄올을 이용하였으며, 연근의 유효성분으로 nuciferine을 확인하였다. 연근 추출물의 화장품소재로서의 응용가능성을 보기 위해 안전성시험(MTT assay), 주름개선 효과시험(Elastase 활성저해 시험) 및 항산화효과시험(DPPH free radical scavenging assay)을 실시하였다. 100% 에탄올로 연근을 추출할 경우 $100{\mu}g/ml$의 농도에서 세포생존율이 80% 이상이어서 연근 추출물은 화장품소재로서 비교적 안전함을 알 수 있었다. 또한 연근 추출물은 $100{\mu}g/ml$의 농도에서 elastase 활성억제가 40~50%를 보여주어서 비교적 우수한 주름개선 효과를 나타내었다. 50% 항산화 저해능을 보이는 농도($FSC_{50}$)는 $5.0{\sim}38{\mu}g/ml$로 연근 추출물의 항산화효과가 매우 우수하였다. 따라서 연근 에탄올추출물은 주름개선 기능성화장품 소재로서 그 가능성이 높음을 알 수 있었다.

• 대한본초학회지
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• 제26권1호
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• pp.111-117
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• 2011

Objectives : The present study was designed to investigate effects of mixed extract from korean medicinal herbs (MIX) on oxidation/reduction reaction-related and aging-related enzyme in vitro. Methods : We performed MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity and xanthine oxidase inhibition assay. Results : Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. The MIX showed 97% inhibition of collagenase activity, and 64% inhibition of elastase activity at 1 mg/ml concentration of MIX, next only to positive control, which indicate good efficacy for anti-wrinkle ingredient. Also it's treatment showed 34% inhibition of tyrosinase activity, to relate whitening effect, at the same dose of MIX. Antioxidant activities were determined by DPPH radical scavenging, xanthine oxidase (XO) inhibiting activity and SOD-like activity. Also these scavenging, XO-inhibiting and SOD-like activities were measured in 91%, 80%, and 63% inhibition, respectively, at a treated dose of 1 mg/ml, compare to control. Conclusions : These results suggest that possibility of mixed korean medicinal herbs as a functional ingredient for anti-wrinkle and whitening, anti-oxidation and anti-aging cosmetic formula.

• 대한화장품학회지
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• 제35권4호
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• pp.317-323
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• 2009

지속적인 자외선 노출은 사람의 피부에 주름 형성을 유발한다. 본 연구에서 생강나무 추출물이 광노화에 의한 피부 주름 형성의 개선에 미치는 효능을 검증해 보고자 하였다. 우선 사람 섬유아세포를 이용하여 생강나무 추출물의 세포증식과 타입 I 콜라겐의 생합성 활성을 측정하였다. 그 결과 생강나무 추출물에 의한 세포증식과 타입 I 콜라겐의 생합성능은 대조군과 비교하여 각각 33.8 %와 91.8 % 증식함을 보였다. 동물실험에서는 SKH-1 무모쥐에 일주일에 3번 UV를 조사하면서 5 % 생강나무 추출액을 국부적으로 도포하였다. 10주 후에는 각각의 무모쥐의 피부 모사판을 제작하여 관찰하였다. 광노화에 의한 주름형성에 미치는 영향을 알아보고자 UVB를 무모쥐의 피부조직에 조사한 후 생강나무 추출액을 도포하여 피부 상태를 관찰하였다. 모사판을 접사카메라를 이용하여 관찰한 결과 5 % 생강나무 추출액의 도포는 생강나무가 포함되지 않은 도포액을 도포한 대조군에 비해 UV에 의해 생성되는 주름 형성 억제에 영향을 주는 것으로 나타났다. 이러한 결과로 생강나무 추출액의 도포는 광노화에 의한 피부주름 생성을 억제하고 피부를 보호하는 효과가 있을 것으로 판단된다.