• Biomolecules & Therapeutics
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• v.12 no.4
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• pp.242-249
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• 2004

The main aim of this study was to investigate stress related activities of Sun-ginseng extract as a candidate for anti-stress-related functional supplement by comparing its effect to those of red ginseng, which is also known to alleviate stress. Normal group was not exposed to stress while the control group was exposed to stress. Rats were orally administered once a day with 200 mg red ginseng (RG) extract, 100 or 200 mg Sun-ginseng (SG) extract/kg body weight. Mice were given water containing 400 mg red ginseng extract, 200 or 400 mg SG/100 mL potable water. Rats were given supplements for 5 days without stress, and 5 days with restraint and electroshock stress. After final stress, stress-related behavioral changes of experimental animals were recorded and levels of blood corticosterone were measured. Mice were given supplements for 5 days through drinking water, and then fatigue related motor activity were recorded. SG-supplementation partially blocked stress effect on locomotion and elevated plus maze test in rats, and also partially blocked stress-induced behavioral changes such as freezing, burrowing, smelling, facewashing, grooming and rearing behavior in rats. SG-supplementation decreased blood corticosterone level which is increased by stress in rats. Effects of SG may not be modulated by GABAnergic nervous system. SG-supplementation prolonged swimming time and staying time on the wire and rotarod wheel in mice. These results suggest that SG partially protects living organisms from stress attack in some cases and thus has the potential to be used as a functional food to alleviate stress response.

• The Journal of Internal Korean Medicine
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• v.22 no.4
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• pp.683-689
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• 2001

In order to study the anti-stress effect of hyangbujapalmultang, several measures of stress, including body weight changes, organ weight changes and catecholamine changes. The following result have been obtained: 1. The weight loss of contol group was $28.5{\pm}1.8g$, that of sample group was $20.3{\pm}1.6g$. This differance was statistcally significant. 2.The organ weight(Liver,Spleen,Kidney,Adrenal gland) was the only significant change in the spleen, in sample group comparing to control group. 3. The norepinephrine contents of control group was $695.5{\pm}22.7pg/ml$, that of sample group was $607.4{\pm}21.7pg/ml$. This shows significant difference in sample group comparing to contol group. 4. The epinephrine contents of control group was $212.8{\pm}9.8pg/ml$, that of sample group was $182.6{\pm}8.4pg/ml$. This shows significant differance in sample group comparing to control group. 5. The dopamine contents of control group was $504.5{\pm}31.3$, that of sample group was $463.4{\pm}27.8pg/ml$. This shows the value decreased, but none of it is significant. Based on avove results, it may be conculed that hyangbujapalmultang has anti-stress effects.

• CELLMED
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• v.13 no.6
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• pp.7.1-7.6
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• 2023

Raphanus sativus L. has been reported to have anti-inflammatory and anti-tumor activity. However, the anti-inflammatory effect and mechanism of action of the Raphanus sativus L. seeds (RSS) with or without oil are still unknown. This study was undertaken to investigate the in-vitro anti-inflammatory effect with or without oil in the RSS on RAW 264.7 cells stimulated by lipopolysaccharide (LPS). Results showed the suppressed LPS-induced secretion of pro-inflammatory mediators such as nitric oxide (NO), inflammatory cytokine (IL-6, TNF-α). Additionally, a decrease in protein expression of iNOS was observed, but nuclear translocation of NF-κB p65 was not inhibited. To elucidate the underlying mechanism of the anti-inflammatory effect of RSS, the involvement of mitogen-activated protein kinase (MAPK) signaling pathways was examined. We also found that RSS blocked LPS-induced phosphorylation of c-Jun N-terminal kinase/stress-activated protein kinase (JNK) signaling but did not affect the phosphorylation of p38 MAPK and extracellular signal-regulated kinase (ERK) 1/2. These results suggest that RSS may have potential as an anti-inflammatory agent through the inhibition of LPS-induced inflammatory cytokine production via regulation of the JNK pathway.

• Journal of Oriental Neuropsychiatry
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• v.24 no.3
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• pp.293-308
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• 2013

Objectives : The Bambusae Caulis in Taeniam has been used in traditional oriental medicine to treat a variety of mental disorders including anxiety and depression. The purpose of present study is to observe the changes which take place in the body in stressful situations and to compare the anti-depressive, anxiolytic and anti-stress effect of Bambusae Caulis in Taeniam in different doses. Methods : This study was performed to evaluate the effects of Bambusae Caulis in Taeniam in the immobilization stress model in rats. Twenty-five rats were divided into 5 groups of 5: control, stress, low dose administration (75 mg/kg), medium dose administration (150 mg/kg) and high dose administration (225 mg/kg). The four groups other than the control group were placed in an immobilization stress test, and distilled water (control) or Bambusae Caulis in Taeniam extract was administration orally for 2 weeks. After treatment, the despair and anxiety behavior of rats were measured by open field test, forced swimming test, weight gain, contents of 5-HT in raphe nucleus and adrenal gland weight. Results & Conclusions : As a result of evaluation by measuring five aspects, FST, OFT, weight gain, 5-HT contents and adrenal gland weight, Bambusae Caulis in Taeniam has significant antidepressant, anxiolytic, anti-stress effects.

• Nutrition Research and Practice
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• v.9 no.3
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• pp.235-241
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• 2015

BACKGROUND/OBJECTIVES: Doenjang, Korean traditional fermented soybean paste has been reported to have an anti-obesity effect. Because adipose tissue is considered a major source of inflammatory signals, we investigated the protective effects of Doenjang and steamed soybean on oxidative stress and inflammation in adipose tissue of diet-induced obese mice. MATERIALS/METHODS: Male C57BL/6J mice were fed a low fat diet (LF), a high-fat diet (HF), or a high-fat containing Doenjang diet (DJ) or a high-fat containing steamed soybean diet (SS) for 11 weeks. RESULTS: Mice fed a DJ diet showed significantly lower body and adipose tissue weights than those in the HF group. Although no significant differences in adipocyte size and number were observed among the HF diet-fed groups, consumption of Doenjang alleviated the incidence of crown-like structures in adipose tissue. Consistently, we observed significantly reduced mRNA levels of oxidative stress markers (heme oxygenase-1 and $p40^{phox}$), pro-inflammatory adipokines (tumor necrosis factor alpha and macrophage chemoattractant protein-1), macrophage markers (CD68 and CD11c), and a fibrosis marker (transforming growth factor beta 1) by Doenjang consumption. Gene expression of anti-inflammatory adipokine, adiponectin was significantly induced in the DJ group and the SS group compared to the HF group. The anti-oxidative stress and anti-inflammatory effects observed in mice fed an SS diet were not as effective as those in mice fed a DJ diet, suggesting that the bioactive compounds produced during fermentation and aging may be involved in the observed health-beneficial effects of Doenjang. CONCLUSIONS: Doenjang alleviated oxidative stress and restored the dysregulated expression of adipokine genes caused by excess adiposity. Therefore, Doenjang may ameliorate systemic inflammation and oxidative stress in obesity via inhibition of inflammatory signals of adipose tissue.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.26 no.2
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• pp.193-198
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• 2017

Rubber materials are widely used for anti-vibration in various industries such as railways, automobile, and aviation. However, various factors hinder the accurate prediction of mechanical properties and lifetime of these materials. Particularly, a stress softening phenomenon Mullins effect greatly affects the accuracy of test results by reducing the initial peak stress. Although the Mullins effect has been studied previously, research on its temperature dependence is lacking. In this study, we performed experiments to estimate the temperature dependence of the Mullins effect. Dumbbell specimens made of natural rubber (NR65) was mounted on a stress softening tester and placed in a heat chamber, where they were tested at temperature of 25, 50, and $80^{\circ}C$. Further, five test sets, each consisting of 10 loading/unloading cycles were sequentially performed at predetermined time intervals. Based on the test results, we assessed the effect of temperature and time interval on stress softening and recovery.

• Journal of the Korean Society of Safety
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• v.32 no.4
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• pp.22-27
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• 2017

In this study, the authors aim to evaluate a structural and fatigue safety of a new type anti-loose nut system with dual nuts composed of main nut and outer nut to enhance the long-term workability and durability so as to improve the performance of conventional anti-loose nut system. Also, a three-dimensional finite-element method analysis was performed to consider the actual geometry and material property of anti-loose nut system with dual nuts and the effect of static and dynamic loads and loading directions. The analytical results showed that the overall static and dynamic stress of the components of the anti-loose nut system with dual nuts were found to be less than that of the fatigue limit of Goodman-smith diagram and allowable stress of each materials, therefore the anti-loose nut system with dual nuts was sufficient to ensure a structural and fatigue safety.

• Journal of the Korean Wood Science and Technology
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• v.43 no.2
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• pp.224-237
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• 2015

We hypothesized that the extract from steam exploded Q. variabilis might be cytoprotective for tenofibroblasts cells during oxidative stress. In the present study, the extracts obtained from steam exploded (severity log Ro 4.68) Q. variabilis contained high quantities of phenolics and flavonoids contents. Also, the extracts appear to have, on these tenofibroblasts, a protective effect against oxidative stress. Tenofibroblasts cells incubated with the extracts and stressed with $H_2O_2$ showed an increase in cell viability by MTT assay. The extracts is found to inhibit $H_2O_2$-induced apoptosis in tenofibroblasts cells, as shown by Annexin V/PI double staining analysis. Western blot data showed that in the extracts/$H_2O_2$-treated cells, the extracts inhibited the $H_2O_2$-dependent phosphorylation of ERK and p38. From these results, it is suggested that the extracts showed the protective effect on $H_2O_2$-mediated oxidative stress. The main chemical compounds of the extract was identified as 1,8-cineole by GC-MS analysis. The anti-apoptosis activity is accordingly believed to be attributable to the 1,8-cineole.

• The Korean Journal of Physiology and Pharmacology
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• v.24 no.5
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• pp.373-384
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• 2020

Paeonol, quercetin, β-sitosterol, and gallic acid extracted from Moutan Cortex had been reported to possess anti-oxidative, anti-inflammatory, and anti-tumor activities. This work aimed to illustrate the potential anti-oxidative mechanism of monomers in human liver hepatocellular carcinoma (HepG2) cells-induced by hydrogen peroxide (H₂O₂) and to evaluate whether the hepatoprotective effect of monomers was independence or synergy in mice stimulated by carbon tetrachloride (CCl₄). Monomers protected against oxidative stress in HepG2 cells in a dose-response manner by inhibiting the generation of reactive oxygen species, increasing total antioxidant capacity, catalase and superoxide dismutase (SOD) activities, and activating the antioxidative pathway of nuclear factor E2-related factor 2/Kelch-like ECH-associated protein 1 (Nrf2/Keap1) signaling pathway. We found that the in vitro antioxidant capacities of paeonol and quercetin were better than those of β-sitosterol and gallic acid. Furthermore, paeonol apparently diminished the levels of alanine transaminase and aspartate aminotransferase, augmented the contents of glutathione and SOD, promoted the expressions of Nrf2 and heme oxygenase-1 proteins in mice stimulated by CCl₄. In HepG2 cells, paeonol, quercetin, β-sitosterol, and gallic acid play a defensive role against H₂O₂-induced oxidative stress through activating Nrf2/Keap1 pathway, indicating that these monomers have anti-oxidative properties. Totally, paeonol and quercetin exerted anti-oxidative and hepatoprotective effects, which is independent rather than synergy.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.4-4
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• 2010

The aim of this study is to investigate anti-stress effect of Nelumbo nucifera extract (NNE) inimmobilization - stressed ICR mice. Stress exposure has induced adrenal gland weight, plasma glucose and level in NNE. The experiments were performed with the use of 9 weeks of age male ICR mice (20-25g) at the time of first treatment with NNE. Animals of the normal group were not exposed to any stress and the control group were exposed to stress. The rats of NNE and Diazepam (BZ) supplementary group were orally administered once a day, 100 mg of NNE, 5 mg of BZ extract/kg body weight and they were exposed to stress. Animals were given supplements for 7 days without stress, and then were given supplements for 5 days with restraining. NNE was effective in decreasing scopolamine induced deficit in passive avoidance test and levels of blood corticosterone which was increased by stress in the mice.