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http://dx.doi.org/10.5658/WOOD.2015.43.2.224

Anti-apoptotic Effect of Steam Exploded Quercus variabilis  

Jo, Jong-Soo (Department of Interior Materials Engineering, Gyeongnam National University of Science and Technology)
Jung, Ji Young (Division of Environmental Forest Science, Institute of Agriculture & Life Scienece, Gyeongsang National University)
Nam, Jeong Bin (Division of Environmental Forest Science, Institute of Agriculture & Life Scienece, Gyeongsang National University)
Park, Hyung Bin (Department of Orthopaedic Surgery, School of Medicine, Gyeongsang National University)
Yang, Jae-Kyung (Division of Environmental Forest Science, Institute of Agriculture & Life Scienece, Gyeongsang National University)
Publication Information
Journal of the Korean Wood Science and Technology / v.43, no.2, 2015 , pp. 224-237 More about this Journal
Abstract
We hypothesized that the extract from steam exploded Q. variabilis might be cytoprotective for tenofibroblasts cells during oxidative stress. In the present study, the extracts obtained from steam exploded (severity log Ro 4.68) Q. variabilis contained high quantities of phenolics and flavonoids contents. Also, the extracts appear to have, on these tenofibroblasts, a protective effect against oxidative stress. Tenofibroblasts cells incubated with the extracts and stressed with $H_2O_2$ showed an increase in cell viability by MTT assay. The extracts is found to inhibit $H_2O_2$-induced apoptosis in tenofibroblasts cells, as shown by Annexin V/PI double staining analysis. Western blot data showed that in the extracts/$H_2O_2$-treated cells, the extracts inhibited the $H_2O_2$-dependent phosphorylation of ERK and p38. From these results, it is suggested that the extracts showed the protective effect on $H_2O_2$-mediated oxidative stress. The main chemical compounds of the extract was identified as 1,8-cineole by GC-MS analysis. The anti-apoptosis activity is accordingly believed to be attributable to the 1,8-cineole.
Keywords
anti-apoptotic; anti-oxidant; Quercus variabilis; steam explosion; achilles tenofibroblasts;
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