• 한국응용과학기술학회지
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• 제34권3호
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• pp.602-612
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• 2017

본 연구에서는 잣나무 잎으로 70% 에탄올 추출물과 증류수 추출물을 얻은 후 항산화 효과, 항염증, 미백효과 등 다양한 효과를 검증하고자 연구를 수행하였다. 항산화 실험 결과, 폴리페놀, 플라보노이드 효과에서는 폴리페놀과 플라보노이드 함량이 농도 의존적으로 증가하였다. 폴리페놀과 플라보노이드 함량은 증류수 추출물에 비해 에탄올 추출물에서 높은 것으로 확인되었다. B16F10, RAW264.7 세포에서 농도별로 세포독성이 나타나지 않았으며, NO 생성 억제능 측정 결과, LPS로 유도된 NO 생성을 효과적으로 억제하여 항염증 활성이 있을 것으로 예측되었다. 멜라닌 생합성 억제능 측정결과, 유의한 감소효과를 확인하였으며, western blot을 수행하여 MITF, Tyrosinase 단백질 발현억제 실험결과, 농도 의존적으로 현저히 억제됨을 확인할 수 있었다. 따라서 본 연구결과로부터 잣나무 잎추출물은 화장품 소재로서의 다양한 활용 가능성이 있을 것으로 사료된다.

• Biomolecules & Therapeutics
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• 제26권3호
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• pp.328-334
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• 2018

Because of the unsatisfactory treatment options for breast cancer (BC), there is a need to develop novel therapeutic approaches for this malignancy. One such strategy is chemotherapy using non-toxic dietary substances and botanical products. Studies have shown that Panduratin A (PA) possesses many health benefits, including anti-inflammatory, anti-bacterial, anti-oxidant and anticancer activities. In the present study, we provide evidence that PA treatment of MCF-7 BC cells resulted in a time- and dose-dependent inhibition of cell growth with an $IC_{50}$ of $15{\mu}M$ and no to little effect on normal human MCF-10A breast cells. To define the mechanism of these anti-proliferative effects of PA, we determined its effect critical molecular events known to regulate the cell cycle and apoptotic machinery. Immunofluorescence and flow cytometric analysis of Annexin V-FITC staining provided evidence for the induction of apoptosis. PA treatment of BC cells resulted in increased activity/expression of mitochondrial cytochrome C, caspases 7, 8 and 9 with a significant increase in the Bax:Bcl-2 ratio, suggesting the involvement of a mitochondrial-dependent apoptotic pathway. Furthermore, cell cycle analysis using flow cytometry showed that PA treatment of cells resulted in G0/G1 arrest in a dose-dependent manner. Immunoblot analysis data revealed that, in MCF-7 cell lines, PA treatment resulted in the dose-dependent (i) induction of $p21^{WAF1/Cip1}$ and p27Kip1, (ii) downregulation of Cyclin dependent kinase (CDK) 4 and (iii) decrease in cyclin D1. These findings suggest that PA may be an effective therapeutic agent against BC.

• Journal of the Korean Wood Science and Technology
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• 제43권2호
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• pp.224-237
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• 2015

We hypothesized that the extract from steam exploded Q. variabilis might be cytoprotective for tenofibroblasts cells during oxidative stress. In the present study, the extracts obtained from steam exploded (severity log Ro 4.68) Q. variabilis contained high quantities of phenolics and flavonoids contents. Also, the extracts appear to have, on these tenofibroblasts, a protective effect against oxidative stress. Tenofibroblasts cells incubated with the extracts and stressed with $H_2O_2$ showed an increase in cell viability by MTT assay. The extracts is found to inhibit $H_2O_2$-induced apoptosis in tenofibroblasts cells, as shown by Annexin V/PI double staining analysis. Western blot data showed that in the extracts/$H_2O_2$-treated cells, the extracts inhibited the $H_2O_2$-dependent phosphorylation of ERK and p38. From these results, it is suggested that the extracts showed the protective effect on $H_2O_2$-mediated oxidative stress. The main chemical compounds of the extract was identified as 1,8-cineole by GC-MS analysis. The anti-apoptosis activity is accordingly believed to be attributable to the 1,8-cineole.

• Toxicological Research
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• 제30권3호
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• pp.211-220
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• 2014

Resveratrol has received considerable attention as a polyphenol with various biological effects such as anti-inflammatory, anti-oxidant, anti-mutagenic, anti-carcinogenic, and cardioprotective properties. As part of the overall safety assessment of HS-1793, a novel resveratrol analogue free from the restriction of metabolic instability and the high dose requirement of resveratrol, we assessed genotoxicity in three in vitro assays: a bacterial mutation assay, a comet assay, and a chromosomal aberration assay. In the bacterial reverse mutation assay, HS-1793 did not increase revertant colony numbers in S. typhimurium strains (TA98, TA100, TA1535 and TA1537) or an E. coli strain (WP2 uvrA) regardless of metabolic activation. HS-1793 showed no evidence of genotoxic activity such as DNA damage on L5178Y $Tk^{+/-}$ mouse lymphoma cells with or without the S9 mix in the in vitro comet assay. No statistically significant differences in the incidence of chromosomal aberrations following HS-1793 treatment was observed on Chinese hamster lung cells exposed with or without the S9 mix. These results provide additional evidence that HS-1793 is non-genotoxic at the dose tested in three standard tests and further supports the generally recognized as safe determination of HS-1793 during early drug development.

• 융합정보논문지
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• 제11권5호
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• pp.190-198
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• 2021

본 연구의 목적은 흑구기자의 생리학적 활성을 평가하여 화장품 산업에 활용 가능한 원료로서의 가능성을 조사하고자 흑구기자 열매에 70% 메탄올(LRM)과 열수(LRW)를 이용하여 추출물을 획득하였다. DPPH와 ABTS 라디컬 소거능 결과 LRW 추출물 보다 LRM 추출물에서 라디컬 소거 활성이 증가하였다. FRAP 분석 결과 LRM 추출물에서 LRW 추출물보다 FRAP 값이 1.3배 증가하였다. 총 폴리페놀 함량은 각각 LRM 추출물에서 31.883±1.395 mg/g, LRW 추출물에서 27.748±2.741 mg/g으로 확인되었다. LRM 추출물은 LPS 자극 후 RAW264.7 세포에서 NO 생성을 억제하였다. 또한 LRM 추출물은 LPS에 의한 COX-2, PGE2, IL-6 및 TNF-α의 발현을 저해하였다. 이러한 결과들로 흑구기자 열매는 화장품에 활용되는 천연 항산화 및 항염 물질로서 가능성이 높을 것으로 기대된다.

• 대한약침학회지
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• 제22권1호
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• pp.16-27
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• 2019

Generative and vegetative parts of the cactuses have had a long-lasting position in folk medicine and their effects could partly be confirmed in scientific experiments. Nowadays, the cactus, fruits, and cladodes are the focus of many studies because of their desirable properties. Therefore, the summarized reports of valuable properties of medicinal plants may be a good way to familiarize researches with a new source of drugs with lower side effects and higher efficacy. Opuntia dillenii, a well-known member of the Cactaceae family, is used as a medicinal plant in various countries and grows in the desert, semi-desert, tropical and sub-tropical areas. It shows diverse pharmacological activities such as: antioxidant, anti-inflammatory, anti-tumor, neuroprotective, hepatoprotective, hypotensive etc. OD fruit also possesses valuable constitutes for instance: betalains, ascorbic acid, total phenol, protein as well as essential elements which suggest the significant potential of this plant as a complementary therapy against several pathological conditions. This review describes experimental evidence about pharmacological and therapeutic potential of OD in order to give the basis of its application in the prevention and treatment of some chronic diseases. More studies on OD can help better understanding of its pharmacological mechanism of action to explain its traditional uses and to identify its potential new therapeutic applications.

• 생명과학회지
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• 제28권9호
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• pp.1048-1055
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• 2018

본 연구에서는 달맞이꽃 종자 물, 에탄올, 메탄올 및 70% 에탄올 추출물의 총 폴리페놀 및 플라보노이드 함량, 항산화, 신경세포 손상 억제, 항암, 대식세포 면역조절 활성 및 에 대하여 비교하였다. 달맞이꽃 종자 추출물 중 메탄올 추출물에서 각각 31.90 mg GAE/g 및 20.66 mg QE/g의 총 폴리페놀 및 총 플라보노이드 함량을 나타내었으며 나머지 추출물과 비교하여 가장 활성이 높았다. 라디칼 소거능 및 환원력 활성은 달맞이꽃 종자 추출물의 처리에 따라 농도의존적으로 증가하였다. 또한 물, 에탄올 및 메탄올 추출물을 $25-100{\mu}g/ml$의 농도로 전처리 하였을 때 글루탐산에 의해 유도되는 신경세포 손상 효과를 감소시키는 것으로 확인되었다. 암세포의 경우 메탄올 추출물이 간암, 폐암, 유방암 및 흑색종 피부암의 세포 생존율을 효과적으로 감소시켜 낮은 $IC_{50}$ 값을 보였으며 대장암과 전립선암에서는 물 추출물과 에탄올 추출물이 가장 낮은 $IC_{50}$ 값을 나타내었다. 또한, 달맞이꽃 종자 70% 에탄올 추출물은 RAW 264.7 마우스 대식세포의 생존율에 영향을 미치지 않는 농도에서 유의적으로 산화질소(nitric oxide, NO)의 생성을 통해 대식세포의 면역활성을 유도하였다. 따라서, 달맞이꽃 종자 추출물은 풍부한 폴리페놀 함량과 다양한 생리활성 효능을 나타내며 이를 통해 달맞이꽃 종자 추출물을 기능성 식품 원료 및 천연물 의약품으로 이용할 수 있을 것으로 사료된다.

• 한국식품저장유통학회지
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• 제23권6호
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• pp.890-898
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• 2016

본 연구의 목적은 갈색거저리의 추출물에 따른 약리활성에 대한 검증 및 효능 평가이다. 갈색거저리의 항산화, 항염증에 대하여 효과를 확인 하였다. 염증 반응은 자극이 가해지면 histamin, serotonin, prostaglandin과 같은 혈관 활성물질에 의해 혈관 투과성이 증대되어 염증을 유발하고 cytokine, free radical, lysosomal enzyme 등 다양한 매개 인자가 관여한다. 자극에 의한 macrophage cell의 염증반응은 tumor necrosis $factor-{\alpha}$($TNF-{\alpha}$), interleukin-6(IL-6), $interleukin-1{\beta}$($IL-1{\beta}$)와 같은 pro-inflammatory cytokine의 발현이 유도되고, inducible nitric oxide synthase(iNOS)와 cyclooxygenase-2(COX-2)에 영향을 받는 유전자의 발현을 자극하게 되어 nitric oxide(NO) 및 $PGE_2$등의 염증 인자가 생성된다. 이에 따라 갈색거지리 추출물의 항염증에 대한 연구를 위해 이에 영향을 주는 인자인 iNOS, COX-2, $PGE_2$, MAPKs의 단백질 발현억제 작용을 확인 하였다. 그 결과 TDW 처리군에서 iNOS 발현율이 19.7%, COX-2의 발현율은 23.2%의 값을 나타내었고, $PGE_2$의 저해 경로를 보기 위해 COX-2의 발현을 mRNA 수준에서 측정한 결과 최고 농도인 $100{\mu}g/mL$에서는 약 60%의 억제효과를 확인할 수 있었다. 결론적으로 TDW는 염증 생성 기전에 작용하여 이 활성을 억제하는데 있어서 효과를 줄 수 있으며 지속적으로 연구해볼 가치가 있다고 사료된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2011년도 임시총회 및 추계학술발표회
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• pp.20-20
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• 2011

In this study, we analyzed 80%MeOH extract of fruits of sorbaria sorbifolia var. stellipila MAX. to measure the total antioxidant capacity by oxygen radical absorbance capacity (ORAC) assay, individual flavonoid content by high-performance liquid chromatography (HPLC). n-Hexane ($1.02{\pm}0.036$), $CH_2Cl_2$ ($0.95{\pm}0.025$), EtOAc ($1.94{\pm}0.065$), n-BuOH ($1.98{\pm}0.054$), D.W. ($1.2{\pm}0.032$) fractions were examined antioxidative activity by ORAC assay. It was revealed that EtOAc($1.94{\pm}0.065$), n-BuOH($1.98{\pm}0.054$) fractions had significant antioxidative activity. The isolation and separation were facilitated using open column chromatography, while separation, purification and identification were accomplished by using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR).

• Asian Pacific Journal of Cancer Prevention
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• 제17권8호
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• pp.3797-3803
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• 2016

Cervical cancer is the second most common malignancy in women worldwide and thus one of the leading causes of mortality in women. Lovastatin, a non polar, anticholesterol drug has previously been reported to exert antitumour activity in vitro. In the present study, lovastatin from Aspergillus terreus (KM017963) was purified by adsoprtion chromatography and evaluated for its anticancer and anti-oxidant properties with a human cervical cancer cell line (HeLa). Growth inhibitory and proapoptotic effects of purified lovastatin on HeLa cells were investigated by determining its influence on cell numbers, mitochondrial membrane potential (MMP), DNA fragmentation and antioxidant properties in terms of hydroxy radical scavenging effects as well as levels of total reduced glutathione. Cell cycle analysis by flow cytometry (propidium iodide staining) confirmed induction of apoptotic cell death and revealed cell cycle arrest in the G0/G1 phase. Results of the study give leads for the anticancer effects of lovastatin and its potential usefulness in the chemotherapy of cervical cancer.