Evaluation of Anti-Oxidant from Natural Products

천연물로부터 유래한 천연 항산화제 규명

  • Kwon, Jin-A (Department of Natural Medicine Resources, Semyung University) ;
  • Yang, Yoon-Jung (Department of Natural Medicine Resources, Semyung University) ;
  • Park, Jong-Hyuk (Department of Natural Medicine Resources, Semyung University) ;
  • Kang, Se-Chan (Department of Natural Medicine Resources, Semyung University)
  • 권진아 (세명대학교 자연약재과학과) ;
  • 양윤정 (세명대학교 자연약재과학과) ;
  • 박종혁 (세명대학교 자연약재과학과) ;
  • 강세찬 (세명대학교 자연약재과학과)
  • Published : 2011.10.21

Abstract

In this study, we analyzed 80%MeOH extract of fruits of sorbaria sorbifolia var. stellipila MAX. to measure the total antioxidant capacity by oxygen radical absorbance capacity (ORAC) assay, individual flavonoid content by high-performance liquid chromatography (HPLC). n-Hexane ($1.02{\pm}0.036$), $CH_2Cl_2$ ($0.95{\pm}0.025$), EtOAc ($1.94{\pm}0.065$), n-BuOH ($1.98{\pm}0.054$), D.W. ($1.2{\pm}0.032$) fractions were examined antioxidative activity by ORAC assay. It was revealed that EtOAc($1.94{\pm}0.065$), n-BuOH($1.98{\pm}0.054$) fractions had significant antioxidative activity. The isolation and separation were facilitated using open column chromatography, while separation, purification and identification were accomplished by using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR).

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