• 한국식품과학회지
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• 제53권4호
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• pp.408-415
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• 2021

본 연구는 커피의 향미개선에 효과가 있다고 알려진 침지 발아를 로부스타 커피에 적용하여 그 유용성을 검증하였다. 베트남 로부스타 커피 생두를 정제수와 함초 추출물에 침지 발아시켜 정수발아(WGC)와 함초발아(SGC) 원두를 제조하고 에스프레소 커피로 추출한 다음 그 기능성을 평가하였다. 400 ㎍/mL 농도에서 Folin-Ciocalteu법으로 분석한 총 폴리페놀 함량은 WGC (20.17%)>NGC (19.45%)>SGC (16.71%) 순으로 WGC의 총 폴리페놀 함량이 가장 높았다. 비발아 커피 뿐만 아니라 발아커피는 25~100 ug/ml 모든 농도에서 DPPH 제거능으로 평가된 실험에서 우수한 항산화 활성을 나타내었다. XOD 저해 효과 또한 400 ㎍/mL 농도에서 WGC 42%, NGC 39%, SGC 32%로 관찰되었다. 비발아 및 발아커피는 멜라닌 생합성의 주요 단계에 관여하는 효소인 tyrosinase 활성을 억제하였으며, α-MSH로 유도된 B16F10 세포에서 멜라닌 생성을 억제하였다. 이 결과는 대조약물 arbutin과 유사한 결과를 나타내었으며, NGC, SGC, WGC는 모두 미백효능이 있는 것으로 확인되었다. Oil red O 염색을 통해 NGC, SGC, WGC의 지방분화 억제효과를 측정한 결과 분화된 대조군 보다 로부스타 커피를 처리한 시료들의 지방구 양이 유의적으로 감소된 것을 확인할 수 있었다. 이상의 연구결과 로부스타 커피의 향미를 개선하기 위해 물 또는 함초 추출물을 사용하여 침지 발아된 커피는 항산화, 미백, 항비만 등 다양한 기능성을 나타내는 것을 시사한다.

• Mycobiology
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• 제40권2호
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• pp.117-123
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• 2012

Nuruk contributes to the unique characteristics of Korean alcoholic beverages. In this study, the effects of nuruk extracts (NE) on anti-oxidant characters, melanogenesis, and anti-photoaging activity were investigated. NEs were obtained from the 70% ethanol extracts of six types of nuruk, which have been used in brewing of fermented alcohol beverages in Korea. First, various antioxidant characteristics were identified in terms of 2,2'-azino-bis(3-ethylbenzthiozoline-6-sulphonic acid) (ABTS) radical scavenging activity, superoxide dismutase (SOD) expression, and inhibition of xanthine oxidase activity. NE#4 exhibited potent ABTS radical scavenging activity ($IC_{50}$ = 19.51 ${\mu}g$/mL). Compared with NE#4, relatively lower levels of activity were observed for NE#3 and NE#6, with $IC_{50}$ values of 90.99 and 76.88 ${\mu}g$/mL, respectively. According to results of western blot analysis for determination of SOD expression in $H_2O_2$-treated HepG2 cells, NE#5 and NE#6 induced a dramatic increase in the expression ratio of SOD, compared to the group treated with $H_2O_2$ only. Activity of xanthine oxidase, which converts xanthine into uric acid, generating superoxide ions, was inhibited by NE#4 and NE#6 in a dose-dependent manner. NE#4 induced significant inhibition of mushroom tyrosinase activity. A reduction in cellular melanin contents of 80% was observed in B16F1 melanocytes treated with NE#5 and NE#6; these effects were similar to those of arbutin at 100 ${\mu}M$. In addition, gelatin zymography and reverse transcription-PCR analysis were performed for assessment of anti-photoaging activity of Nuruk. Treatment with NE#6 resulted in dramatically inhibited activities of matrix metalloproteinase (MMP)-2/-9, suppressed expression of MMP-1, and increased expression of type-1 procollagen. Results of gelatin zymography for NE#4 and NE#5 were similar, to a slightly lesser degree. These results suggest the potential of NE#4 and NE#6 as natural ingredients for use in functional foods and cosmetics.

• 한국축산식품학회지
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• 제34권6호
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• pp.844-851
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• 2014

This study focused on the anti-oxidative and collagenase- and elastase inhibition effects of low molecular weight peptides (LMP) from commercial Jeju horse leg bone hydrolysates (JHLB) on pancreatin, via enzymatic hydrolysis. Cell viability of dermal fibroblasts exposed to UVB radiation upon treatment with LMP from JHLB was evaluated. Determination of the antioxidant activity of various concentrations of LMP from JHLB were carried out by assessing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethybenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC). The DPPH radical scavenging activity of LMP from JHLB (20 mg/mL) was 92.21% and ABTS radical scavenging activity (15 mg/mL) was 99.50%. FRAP activity (30 mg/mL) was $364.72{\mu}M/TE$ and ORAC activity (1 mg/mL) was $101.85{\mu}M/TE$. The anti-wrinkle potential was assessed by evaluating the elastase- and collagenase inhibition potential of these LMP. We found that 200 mg/mL of LMP from JHLB inhibited elastase activity by 41.32%, and 100 mg/mL of LMP from JHLB inhibited collagenase activity by 91.32%. The cell viability of untreated HS68 human dermal fibroblasts was 45% when exposed to a UVB radiation dose of $100mJ/cm^2$. After 24 h of incubation with $500{\mu}g/mL$ LMP from JHLB, the cell viability increased to 60%. These results indicate that LMP from JHLB has potential utility as an anti-oxidant and anti-wrinkle agent in the food and cosmetic industry. Additional in vivo tests should be carried out to further characterize these potential benefits.

• 대한본초학회지
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• 제29권4호
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• pp.13-20
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• 2014

Objectives : Atotang was composed of 10 kinds of traditional medicinal herb. This research was performed to examine biological effects of Atotang for the development of prescription on atopic dermatitis. Methods : Atotang was extracted with 80% EtOH. Free radical scavenging assay has tested for anti-oxidative activity as well as the contents of total polyphenol. We observed the production of ROS, nitric oxide(NO) and the inflammatory cytokines such as interleukin-1beta(IL-${\beta}$), IL-6, tumor necrosis factor-alpha(TNF-${\alpha}$), Prostaglandin E2($PGE_2$) in Raw 264.7 cells stimulated by LPS. We used Disc diffusion method to investigate antibacterial activity on Candida albicans, Staphylococcus aureus and Staphylococcus epidermis. Result : Content of total phenolic compound of Atotang was 36.3 mg/g ext. DPPH and ABTS scavenging activities were 77% and 46% at 200 ug/ml respectively, showing dose-dependent increase. The amounts of ROS and NO in RAW 264.7 cells were decreased by 30% and 19% at 200 ug/ml, respectively, showing dose-dependent decrease. The prodcution of IL-1beta, IL-6 and TNF-alpha in RAW 264.7 cells were decreased dose-dependently by 81%, 67%, and 20% at 200 ug/ml, respectively. Atotang was reduced LPS-stimulated production of $PGE_2$ by 33%. Atotang on C. albicans, S. aureus and S. epidermis was selected by a disc diffusion method and inhibition effect of the Atotang on the growth of S. epidermis was the greatest. Conclusion : The results indicated that Atotang showed biological activities showing anti-oxidant, anti-inflammatory and antibacterial effects. Based on these results, it is concluded that Atotang can be applied to the prescription on atopic dermatitis.

• 셀메드
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• 제4권1호
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• pp.7.1-7.8
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• 2014

Metabolic effects of ten daily doses of standardized extract of Andrographis paniculata leaves (AP) rich in andrographolide were evaluated in a rat model of type-2 diabetes and in diet induced obese rats. AP was administered per-orally as suspension in 0.3% carboxymethylcellulose at doses of 50, 100 and 200 mg/kg/day for 10 consecutive days. Blood glucose, insulin and lipid profile of rats were measured by using enzyme kits. In addition, effects of such treatments on anti-oxidant enzymes activity and histopathological changes in various organs of diabetic rats were assessed. AP treatments reversed body weight losses and increased plasma insulin level in diabetic rats. The anti-oxidant enzymes activity became normal and histopathological changes observed in pancreas, liver, kidney and spleen of diabetic animals were less severe in extract treated groups. On the other hand, hyperinsulinemia and increased body weight gains observed in high fat or fructose fed rats were less severe in the extract treated groups. These observations revealed therapeutic potentials of the extract for treatments of diabesity associated metabolic disorders, and suggest that the effects of the extract on insulin homeostasis depend on the metabolic status of animals. Activation of cytoprotective mechanisms could be involved in its mode of action.

• Natural Product Sciences
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• 제23권3호
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• pp.217-221
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• 2017

Eleven compounds (1-11) were isolated from the rhizomes of Osmunda japonica, and their structures were elucidated based on $^1H$, $^{13}C-NMR$ and LC-IT-TOF MS data. Of these compounds, all compounds (1 - 11) have been previously reported, although five (6 - 9, 11) have not previously been isolated from this plant. The antioxidant activities of isolated compounds (1 - 11) were measured by DPPH and ABTS assays, and compound 10 showed the high antioxidant activity.

• Natural Product Sciences
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• 제25권2호
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• pp.122-129
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• 2019

The roots of Phlomis umbrosa (Turcz.) (Phlomidis Radix) have been traditionally used to treat cold, reduce swelling and staunch bleeding. Four iridoids (1 - 3 and 5) and six phenylethanoid derivatives (4, and 6 - 10) were isolated from the roots of P. umbrosa. A simple, sensitive, and reliable analytical HPLC/PDA method was developed, validated, and applied to determine 10 marker compounds in Phlomidis Radix. Furthermore, the isolates were evaluated for cytotoxic and anti-oxidant activities as well as DPPH-HPLC method. Among them, compounds 4 and 6 - 9 displayed potent anti-oxidant capacities using DPPH assay with $IC_{50}$ values of $27.7{\pm}2.4$, $10.2{\pm}1.1$, $18.0{\pm}0.8$, $19.1{\pm}0.3$, and $19.9{\pm}0.6{\mu}M$, and compounds 6, 8, and 9 displayed significant cytotoxic activity against HL-60 with $IC_{50}$ values of $35.4{\pm}3.1$, $18.6{\pm}2.0$, and $42.9{\pm}3.0{\mu}M$, respectively.

• Mycobiology
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• 제51권3호
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• pp.169-177
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• 2023

To further explore the molecular mechanism of triterpenoid biosynthesis and acquire high-value strain of Sanghuangporus baumii, the Agrobacterium tumefaciens-mediated transformation (ATMT) system was studied. The key triterpenoid biosynthesis-associated gene isopentenyl diphosphate isomerase (IDI) was transformed into S. baumii by ATMT system. Then, the qRT-PCR technique was used to analyze gene transcript level, and the widely targeted metabolomics was used to investigate individual triterpenoid content. Total triterpenoid content and anti-oxidant activity were determined by spectrophotometer. In this study, we for the first time established an efficient ATMT system and transferred the IDI gene into S. baumii. Relative to the wild-type (WT) strain, the IDI-transformant (IT) strain showed significantly higher transcript levels of IDI and total triterpenoid content. We then investigated individual triterpenoids in S. baumii, which led to the identification of 10 distinct triterpenoids. The contents of individual triterpenoids produced by the IT2 strain were 1.76-10.03 times higher than those produced by the WT strain. The triterpenoid production showed a significant positive correlation with the IDI gene expression. Besides, IT2 strain showed better anti-oxidant activity. The findings provide valuable information about the biosynthetic pathway of triterpenoids and provide a strategy for cultivating high-value S. baumii strains.

• 한국식품저장유통학회지
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• 제24권5호
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• pp.638-646
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• 2017

본 연구에서는 복숭아의 품종인 미황, 가납암백도 및 천홍을 성숙정도에 따라 미숙과와 성숙과로 분류하여 항산화 및 항염증 효과간의 상관관계 등을 분석함으로서 향후 염증 및 산화스트레스와 관련된 질병의 예방 또는 치료할 수 있는 기능성 소재를 발굴하고자 수행하였다. 총 페놀 및 플라보노이드 함량은 3품종 모두 성숙과에 비해 미숙과에서 함량이 많았고, 미숙과 중에서는 천홍이, 성숙과 중에서는 미황이 유의성 있게 높은 함량을 나타내었다. 항산화 활성은 미숙과 천홍에서 가장 높았고, 성숙과에서는 DPPH 라디칼 소거능 측정에 의해서는 미황이 유의성 있게 높았으나 FRAP과 CUPRAC 측정에서는 3품종간 유의적인 차이가 없었다. RAW 264.7 세포에서는 미황 미숙과 추출물이 약 30%의 NO 생성 억제 효과가 관찰되었고, 가납암백도 미숙과는 약 29%, 천홍 미숙과는 약 20%의 저해활성을 보이지만, 복숭아 성숙과 3품종에서는 NO 생성 억제 효과가 나타나지 않았다. 3품종 모두 성숙과에 비해 미숙과에서 저해 활성이 높게 나타났다. 총 페놀 함량과 플라보노이드 함량에 따른 항산화 활성들 간의 상관관계에서 고도의 정의 상관성을 나타내었으며, 항염증 활성과 항산화 활성, 항염증 활성과 총 페놀 및 플라보노이드 함량은 낮은 상관관계를 나타내었다.

• 대한화장품학회지
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• 제32권3호
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• pp.161-171
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• 2006

본 연구에서는 제주도에서 자생하는 37 종의 식물추출물에 대한 미백 및 항산화 효능을 조사하였다. 미백 효능은 in vitro tyrosinase 저해활성과 B16-F1 세포를 이용한 멜라닌 합성 저해효과를 조사하여 확인하였고, 항산화 효능은 DPPH (1,1-diphenyl-2-picrylhydrazyl)를 이용한 전자공여능 측정방법과 DCF-DA (dichlorofluorescin diacetate)를 이용한 V79-4 폐 섬유아세포에서 활성 산소종 소거작용 능력을 조사하여 측정하였다. 세포실험 조건에서 식물추출물들의 투여농도에 따른 세포독성 정도를 조사하기 위하여 MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay를 수행하였다. 미백 효능조사 실험에서 우수한 결과를 나타내었던 8종의 식물 추출물들은 토끼를 이용한 국소 독성 시험 및 인체 피부 자극 임상 시험에 적용하여 안전성을 조사하였다. 연구결과에서 높은 미백과 항산화 효능을 동시에 나타내었던 비수리(Lespedeza cuneata), 제주광나무(Ligustrum lucidum (stem)), 산뽕나무(Morus bombycis (stem)), 꿀풀(Prunella vulgaris var. lilacina)의 식물추출물들은 화장품 원료화에 매우 유리한 조건을 갖고 있음을 확인하였다.