The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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An experimental study for the development of prescription on atopic dermatitis

아토피 피부염 외치치료제 처방 개발을 위한 실험적 연구

  • Kim, Gun Woo (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Bak, Ji Won (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Sim, Boo-Yong (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Kim, Dong Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
  • 김건우 (대전대학교 한의과대학 병리학교실) ;
  • 박지원 (대전대학교 한의과대학 병리학교실) ;
  • 심부용 (대전대학교 한의과대학 병리학교실) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Received : 2014.06.30
  • Accepted : 2014.07.16
  • Published : 2014.07.30
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Abstract

Objectives : Atotang was composed of 10 kinds of traditional medicinal herb. This research was performed to examine biological effects of Atotang for the development of prescription on atopic dermatitis. Methods : Atotang was extracted with 80% EtOH. Free radical scavenging assay has tested for anti-oxidative activity as well as the contents of total polyphenol. We observed the production of ROS, nitric oxide(NO) and the inflammatory cytokines such as interleukin-1beta(IL-${\beta}$), IL-6, tumor necrosis factor-alpha(TNF-${\alpha}$), Prostaglandin E2($PGE_2$) in Raw 264.7 cells stimulated by LPS. We used Disc diffusion method to investigate antibacterial activity on Candida albicans, Staphylococcus aureus and Staphylococcus epidermis. Result : Content of total phenolic compound of Atotang was 36.3 mg/g ext. DPPH and ABTS scavenging activities were 77% and 46% at 200 ug/ml respectively, showing dose-dependent increase. The amounts of ROS and NO in RAW 264.7 cells were decreased by 30% and 19% at 200 ug/ml, respectively, showing dose-dependent decrease. The prodcution of IL-1beta, IL-6 and TNF-alpha in RAW 264.7 cells were decreased dose-dependently by 81%, 67%, and 20% at 200 ug/ml, respectively. Atotang was reduced LPS-stimulated production of $PGE_2$ by 33%. Atotang on C. albicans, S. aureus and S. epidermis was selected by a disc diffusion method and inhibition effect of the Atotang on the growth of S. epidermis was the greatest. Conclusion : The results indicated that Atotang showed biological activities showing anti-oxidant, anti-inflammatory and antibacterial effects. Based on these results, it is concluded that Atotang can be applied to the prescription on atopic dermatitis.

Keywords

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