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Preliminary Screening of Some Jeju Island Native Plants for Whitening and Antioxidant Activity  

Yoo, Byoung-Sam (Cosmetic R&D Center, Cosmax Inc.)
Moon, Ji-Young (Cosmetic R&D Center, Cosmax Inc.)
Kim, Ju-Ho (Cosmetic R&D Center, Cosmax Inc.)
Hyun, Jin-Won (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
Kang, Kyoung-Ah (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
Koh, Jea-Sook (Dermapro Co., LTD.)
Seo, Young-Kyoung (Dermapro Co., LTD.)
Baek, Ji-Hwoon (Dermapro Co., LTD.)
Park, Deok-Hoon (Biospectrum Life Science Institute)
Lee, Jong-Sung (Biospectrum Life Science Institute)
Jung, Eun-Sun (Biospectrum Life Science Institute)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.32, no.3, 2006 , pp. 161-171 More about this Journal
Abstract
In this study, we investigated the whitening and anti-oxidant activity of 37 Jeju island native plants. The active ingredients of the plants were prepared by methanol extraction. Whitening activity of plant extracts was examined from the inhibitory effect of tyrosinase and the inhibition of melanin synthesis of the B16-F1 cell line. Their anti-oxidant activity was measured by electron donating ability of DPPH (1,1-diphenyl-2-picrylhydrazyl) and ROS (reactive oxygen species) scavenging activity in V79-4 lung fibroblast cells using DCF-DA (dichlorofluorescin diacetate). Cytotoxicity of the extracts on cell s based experiments was investigated by MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay. Also, the toxicity test using a rabbit and the human skin patch test were carried out for examining the safety of the extracts which showed the high whitening activity. It is interesting that the extracts of Lespedeza cuneata, Ligustrum lucidum (stem), Morus bombycis (stem) and Prunella vulgaris var. lilacina showed both potent whitening and anti-oxidant activities.
Keywords
tyrosinase; DPPH; DCF-DA; MTT; skin-patch test;
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  • Reference
1 K. Maeda and M. Fkuda, In vitro effectiveness of several whitening cosmetic components in hwnan melanocyte, J. Soc. Cosmet. Chem., 42, 361 (1991)
2 박수남, 천연물의 피부세포에 미치는 영향, 대한화장품학회지, 25(1) (1999)
3 S. K. Khan, R. Agarwal and H. Mukhtar, Inhibition of 12-O-tetradecanoyl-phorobol-13-acetate tumor promotion in 7,12-dimethyben z[$\alpha$]antracene-initiated SENCER mouse skin by a polyphenolic fraction isolated from green tea., Cancer Res. 52, 6890 (1992)
4 김보건, 제주자생 약용식물의 활성 및 성분에 관한 조사 연구, 제주대학교 교육대학원 석사학위논문 (2002)
5 P. Bernard, N. Boone, E. Amoult, T. Scior, and Q. T. Do, Preliminary screening of some tropical plants for anti-tyrosinase activity, J. Ethnophurmcology, 82, 155 (2002)   DOI   ScienceOn
6 T. Mosmann, Rapid colorimetric assay for the cellular growth and survival : application to proliferation and cytotoxic assay, J. Immun Methods, 65, 55 (1983)   DOI
7 S. F. Lo, S. M. Mulabagal, S. Matthew, C. L. Chen, C. L. Kuo, and H. S. Tsay, In vitro propagation by asymbiotic seed germination and 1,1-diphenyl-2-picrylhydyazyl(DPPH) radical scavenging activity studies on tissue culture raised plants of three medicinally important species of Dendrobium, Biol. Pharm. Bull., 27, 731 (2004)   DOI   ScienceOn
8 S. T. Omaye, K. A. Reddy, and C. E. Cross, Effect of butylated hydroxytoluene and other antioxidants on mouse lung metabolism, J. Toxicol. Environ Health, 3, 829 (1977)   DOI
9 김정호, HS-시리즈의 미백 효과와 tyrosinase의 활성 저해 연구, 부산대학교 대학원 석사 학위 논문 (2005)
10 H. Masaki, S. Sakaki, T. Atsumi and H. Sakurai, Active-oxygen scavenging activity of plant extracts, Biol. Pharm. Bull., 18, 162 (1995)   DOI   ScienceOn
11 A. R. Rosenkranz, S. Schmaldienst, K. M. Stuhlmeier, W. Chen, W. Knapp, and G. J. Zlabinger, A microplate assay for the detection of oxidative products using 2',6' -dichlorofluorescein-diacetate, J. Immunl. Methods, 156, 39 (1992)   DOI   ScienceOn
12 T. Hatano, Constituents of natural medicines with scavenging effects on active oxygen speciestannins and related polyphenols, Natural Medicines, 49, 357 (1995)
13 R. Lutan and D. Lutan, Stimulation of melanogenesis in a human melanoma cell line by retinoids, Cancer Res., 40, 3345 (1980)
14 K. Maeda and M. Fkuda, Mechanism of its depigmentation in human melanocyte culture, American Soc. Pharm. Exp. Therapeutics, 276(2), 765 (1996)
15 S. Marklund and G. Marklund, Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase, Eur. J. Biochem., 47, 469 (1974)   DOI   ScienceOn
16 김문홍, 제주식물도감, 제주도인쇄협동조합 (1992)
17 M. Iwata, T. Com, S. Iwata, M. Evertte, and B. B. Fuller, The relationship between tyrosinase activity and skin color in human foreskins, J. Invest Dermatol., 195, 9 (1990)
18 안명수, 원종숙, 솔잎추출물의 옥배유에 대한 항산화 효과, 성신여대 생활문화연구, 83 (2001)
19 김원희, 천연식물 추출물의 멜라닌 생성 저해에 관한 연구, 덕성여자대학교 대학원 석사학위 논문, 45 (2003)
20 A. L. Branen, Toxicology and biochemistry of butylated hydroxytoluene, J. AM. Oil Chem. Soc., 52, 59 (1975)   DOI
21 C. Koide, M. Senoo, and I. Hoshino, Melanogenesisinhibitory effect of kojic acid-glabridintoil soluble licorice extract) composite, Fragrance J., 25(9), 43 (1997)