• Title/Summary/Keyword: Anti-histamine

Search Result 332, Processing Time 0.024 seconds

Galgeun-tang, an Herbal Formula, Ameliorates Atopic Dermatitis Responses in Dust Mite Extract-treated NC/Nga Mice

  • Ha, Hyekyung;Lee, Jun Kyoung;Lee, Mee-Young;Lim, Hye-Sun;Shin, Hyeunkyoo
    • The Journal of Korean Medicine
    • /
    • v.34 no.4
    • /
    • pp.1-11
    • /
    • 2013
  • Objectives: Galgeun-tang (GGT, gegen-tang, kakkon-to), an herbal formula, is used to treat the common cold, fevers, headaches, hangovers and neck and upper back stiffness. The drugs currently used to treat atopic dermatitis (AD) are limited by the significant adverse effects associated with their long-term usage. The need to efficiently manage the AD response while reducing side effects has led to the development of alternative remedies. Methods: To assess the effects of GGT on AD, the anti-inflammatory and anti-AD properties of GGT were evaluated in both in vitro and in vivo systems. Results: Nitric oxide (NO) and histamine production on lipopolysaccharide (LPS)-treated RAW264.7 cells and phorbol-12 myristate 13-acetate (PMA)/A23187-treated MC/9 cells, respectively, were inhibited by GGT. GGT reduced thymus and activation-regulated chemokine (TARC/CCL17) release on TNF-${\alpha}$/IFN-${\gamma}$ stimulated HaCaT cells in a dose-dependent manner. GGT reduced both plasma levels of IgE and histamine and the dermatitis score in house dust mite induced atopic dermatitis-like lesions on NC/Nga mice. However, there were no significant histopathological differences observed between the GGT group and the AD-induced group, such as AD-like lesions in the dorsal skin or ear or mast cell infiltration in the dorsal skin. Conclusions: These results indicate that GGT inhibits chemokine production by keratinocytes and the atopic dermatitis response in NC/Nga mice, suggesting that GGT may be useful as a therapeutic remedy for treating AD and allergic inflammation-related diseases.

Experimental Study of Inhibitory Effect on Anaphylactic Shock of Sosihotang by Anal Therapy (항장요법(肛腸療法)에 의한 소시호탕(小柴胡湯)의 아나필락시 쇼크 억제(抑制)에 대한 실험적(實驗的) 연구(硏究))

  • Kang, Tai-Hee;Moon, Seok-Jae;Moon, Goo;Won, Jin-Hee;Kim, Hyoung-Min
    • The Journal of Korean Medicine
    • /
    • v.19 no.2
    • /
    • pp.439-449
    • /
    • 1998
  • Anal Therapy is another way of taking medicine. It is a traditional pathway but not available in common situation. Nevertheless, It has many benifect and usefulness, it has not treated so much. Through Anal Therapy, the valid compound of Herb med can be reach to the desination in theory of the organism and loca1 medical action. The former is called Jung-Chei Theory(整體論), which is the one of the most important basements in building traditional Korean medicine. As there are many kinds of Anal therapy, this study use reservation type. Sosihotang(SSHT) is one of the well-known korean medicines for a long time. It is used for the treatment of such dieases as infectious diseases, hepatic diseases and gastroenteritis and so on. In this study, the author investigated the effect of an aqueous extract of SSHT by Anal therapy(Reservative Enema) in anaphylactic shock. The following results were obtained 1. SSHT inhibited anaphylactic shock 100% with a dose of 1.0 g/kg 1 hr before intraperitoneal injection of compound 48/80. SSHT significantly reduced serum histamine contents induced by compound 48/80. 2. SSHT (0.1 g/kg) also inhibited to 30.9% (P<0.05)) local cutaneous anaphylactic reaction activated by anti-dinitrophenyl (DNP) IgE. 3. The validity rate of reservative enema is as much as oral pathway. 4. In addition, SSHT dose-dependently inhibited the histamine release from the peritoneal mast cells by compound 48/80 or anti-DNP IgE. These results provide evidence that Anal Therapy(Reservative enema) of SSHT may be beneficial in the treatment of systemic and local anaphylactic reaction. Moreover, I wish another much sincere study of Anal Therapy (Reservative enema) would be obtained.

  • PDF

Allergenicity Test of Genetically Modified Soybean in Sprague Dawley Rats

  • Chang, Hyun-Sung;Bae, Youn-Kyoung;Lim, Si-Kyu;Jeong, Tae-Cheon;Kim, Hyung-Soo;Chung, Seung-Tae;Kim, Dong-Sup;Nam, Doo-Hyun
    • Archives of Pharmacal Research
    • /
    • v.24 no.3
    • /
    • pp.256-261
    • /
    • 2001
  • Allergenicity of genetically-modified (GM) soybean was evaluated in male Sprague Dawley rats. To confirm the GM soybean used in this study, the polymerase chain reaction (PCR) was performed using the chromosomal DNA of soybeans. The PCR result provided the clear discrimination of genetically-modified (GM) soybeans. To evaluate the allergenicity of GM soybean and non-GM control one, the soybean homogenate was sensitized subcutaneously 3 times a week for 3 weeks. The doses of soybean were 0, 2 and 20 mg/kg in the protein basis. A week after the last sensitization, antisera were recovered from individual animals. When the sera were injected intradermally on the clipped back of unsensitized rats with various dilutions, followed by a challenge with 20 mg/kg of soybean homogenate containing 1% Evans blue, no sign of passive cutaneous anaphylaxis reaction was detected. In addition, when the sera were treated in the cultures of peritoneal mast cells, the increase of histamine release by anti-(GM soybean) sera was not observed when compared to that by anti-(non-GM soybean) sera. The present results indicate that the GM soybean might not act as a strong allergen in male Sprague Dawley rats.

  • PDF

Inhibitory Effect of Artemisiae Asiaticae Herba on Degranulation, Production of Cytokine, and $Fc{\varepsilon}RI$ Expression in RBL-2H3 Cells (애엽(艾葉)의 RBL-2H3 비만세포 탈과립, cytokine 생산 및 $Fc{\varepsilon}RI$ 유전자 발현에 미치는 영향)

  • Ban, Hak Jin;Park, Dong II;Kang, Kyung Hwa
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.26 no.6
    • /
    • pp.915-921
    • /
    • 2012
  • Artemisiae Asiaticae Herba (AAH) has been used to remedy of symptoms such as bleeding, dysmenorrhea, eczema and itchy skin in Oriental Medicine. In this study, we investigated the protective effect of AAH on allergic response. The effect of AAH was analyzed by ELISA and RT-PCR in RBL-2H3 cells. We investigated cell viability, ${\beta}$-hexosaminidase and histamine as markers of degranulation, production of IL-4 and TNF-${\alpha}$, and gene expression of HDC2, cytokines and $Fc{\varepsilon}RI$ ${\alpha}{\beta}{\gamma}$ subunit. We found that AAH suppressed ${\beta}$-hexosaminidase and histamine release, the production of IL-4 and TNF-${\alpha}$ in RBL-2H3 by the anti-DNP IgE plus DNP-HSA stimulation. AAH also significantly decreased cytokine mRNA expressions, such as IL-$1{\beta}$, IL-2, IL-3, IL-4, IL-5, IL-6, IL-12, IL-13, TNF-${\alpha}$, and GM-CSF, and increased cytokine mRNA expressions of IL-10 in RBL-2H3. In addition, AAH suppressed mRNA expression of $Fc{\varepsilon}RI$ ${\alpha}{\beta}{\gamma}$ subunit on cell surface. Our results indicate that AAH protects against allergic response and exerts an anti-inflammatory effect through the inhibition of degranulation and production of cytokines and expression of $Fc{\varepsilon}RI$ ${\alpha}{\beta}{\gamma}$ subunit.

Ribes fasciculatum var. chinense Attenuated Allergic Inflammation In Vivo and In Vitro

  • Jung, Ji-Wook;Kim, Su-Jin;Ahn, Eun-Mi;Oh, Sa-Rang;Lee, Hye-Ja;Jeong, Ji-Ahn;Lee, Ju-Young
    • Biomolecules & Therapeutics
    • /
    • v.22 no.6
    • /
    • pp.547-552
    • /
    • 2014
  • Ribes fasciculatum var. chinense MAX. (R. fasciculatum) has traditionally been used in Korea to treat inflammatory diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of R. fasciculatum is not completely understood. We aimed to ascertain the pharmacological effects of R. fasciculatum on both compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of R. fasciculatum, we evaluated the effects of R. fasciculatum on the production of inflammatory mediators in LPS-stimulated macrophage cells. Treatment of R. fasciculatum significantly reduced compound 48/80- or histamine-induced the pruritus in mice. R. fasciculatum attenuated the AD symptoms such as eczematous, erythema and dryness and serum IgE levels in AD model. Additionally, R. fasciculatum inhibited the production of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-6 (IL-6). The maximal rates of TNF-${\alpha}$ and IL-6 inhibition by R. fasciculatum (1 mg/ml) were approximately 32.12% and 46.24%, respectively. We also showed that R. fasciculatum inhibited the activation of nuclear factor-kappa B in LPS-stimulated macrophages. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of R. fasciculatum as a potential molecule for use in the treatment of allergic inflammatory diseases.

Suppressing Effects of Ethanol Extract from Dioscoreae Rhizoma on atope Dermatitis (부채마 에탄올 추출물의 아토피 피부염 억제 효과)

  • Do, Young-Bog;Song, Young-Soon
    • Journal of Digital Convergence
    • /
    • v.17 no.8
    • /
    • pp.257-264
    • /
    • 2019
  • This study was conducted to investigate the effect of 70% ethanol extract (DR) on the mast cell-mediated allergic contact dermatitis induced by dinitrofluorobenzene in BALB / c mice, which affects the cell activity by antigen in RBL-2H3 mast cells Respectively. The ethanol extracts of RBL-2H3 cells activated by DNP-HSA and anti-DNP IgE antibodies inhibited the secretion of ${\beta}$-hexosaminidase, histamine, and IL-4 and $TNF-{\alpha}$ Production was suppressed. In the DNFB-induced contact allergic dermatitis animal model, treatment with ethanol extract reduced ear swelling and inhibited serum histamine and IL-4 secretion, and DR treatment effectively prevented mast cell infiltration in dermatitis-induced areas. As a result, the ethanol extract may be used as a therapeutic agent for mast cell-mediated allergic diseases such as atopic dermatitis.

Kaempferol Isolated from Nelumbo nucifera Stamens Negatively Regulates $Fc{\varepsilon}RI$ Expression in Human Basophilic KU812F Cells

  • Shim, Sun-Yup;Choi, Jae-Sue;Byun, Dae-Seok
    • Journal of Microbiology and Biotechnology
    • /
    • v.19 no.2
    • /
    • pp.155-160
    • /
    • 2009
  • Mast cells and basophils perform important functions as pivotal effector cells in IgE-mediated allergic reactions. KU812F cells, a human basophilic cell line isolated originally from chronic myelocytic leukemia, express a high affinity receptor of IgE, $Fc{\varepsilon}RI$. Kaempferol was extracted and isolated from a methanolic extract of flavonoid-rich Nelumbo nucifera stamens. In the present study, the inhibitory effects of kaempferol on $Fc{\varepsilon}RI$ expression in human basophilic KU812F cells was examined. Flow cytometric analysis revealed that $Fc{\varepsilon}RI$ expression on the cell surface was suppressed in a concentration-dependent manner when the cells were cultured with kaempferol. Moreover, RT-PCR analysis showed that the mRNA levels for $Fc{\varepsilon}RI$ ${\alpha}-\;and\;{\gamma}$-chains were reduced as the result of kaempferol treatment in a concentration-dependent manner. Kaempferol showed its suppressive effects on intracellular $Ca^{2+}$ concentration and histamine release from anti-$Fc{\varepsilon}RI$ ${\alpha}$-chain antibody-stimulated cells in a concentration-dependent manner. These observations indicate that kaempferol may exert antiallergic effect via down regulation of $Fc{\varepsilon}RI$ expression and degranulation.

Inhibitory Effects of Water Extract of Lindera obtusiloba on the Mast Cell-Mediated Allergic Inflammation (생강나무 추출물의 알레르기성 염증반응 억제 효과)

  • Kim, Sang-Hyun;Son, Jun-Ho;Lee, Seung-Ho
    • Korean Journal of Pharmacognosy
    • /
    • v.40 no.3
    • /
    • pp.233-237
    • /
    • 2009
  • Lindera obtusiloba has been used for centuries as a traditional medicine in Korea and recently known to have an anti-fibrotic effect. In this report, we investigated the effect of hot water extract from L. obtusiloba (WELB) on the mast cell-mediated allergic inflammation and studied its possible mechanisms of action. WELB inhibited phorbol-12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-induced histamine release in HMC-1 human mast cells. WELB reduced PMACI-induced gene expression and secretion of proinflammatory cytokines such as tumor necrosis factor-$\alpha$, interleukin (IL)-$1{\beta}$, IL-6, and IL-8. The inhibitory effect of WELB on the expression of proinflammatory cytokines was c-jun N-terminal kinase and nuclear factor-${\kappa}B$ dependent. These results indicate that WELB may be beneficial in the treatment of mast cell-mediated allergic inflammation.

Effect of Bopaewon-tang on Allergic Reaction (보폐원탕(補肺元湯)이 알러지반응에 미치는 영향)

  • Jeon Yong-Keun;Leem Jae-Yoon;Song Jung-Mo;Eun Jae-Soon
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.19 no.6
    • /
    • pp.1604-1609
    • /
    • 2005
  • The purpose of this research was to investigate the effects of Bopaewon-tang (BT) on allergic reaction. In the present study, we examined the effect of BT on type 1 and type tV allergic reaction. BT (500 mg/kg) did not affect the systemic anaphylaxis induced by compound 48180 and the passive cutaneous anaphylaxis induced by anti-dinitrophenyl (DNP)-IgE and DNP-human serum albumin in vivo. Also, BT did not affect the release of histamine from peritoneal mast cells in rats. In addition, BT did not affect the permeability of evans blue into peritoneal cavity, but inhibited the writhing syndrome induced by acetic acid. BT inhibited the delayed type hypersensitivity induced by SRBC and the contact dermatitis induced by dinitrofluorobenzene. These results indicate that BT may be useful for the prevention and treatment of type IV allergy related disease.

Effect of Polyphenol Compounds from Persimmon Leaves (Diospyros kaki folium) on Antiallergy (감잎(Diospyros kaki folium) Polyphenol 화합물군의 알레르기 저해효과)

  • Park, Mu-Hee;Choi, Cheong;Son, Gyu-Mok;An, Bong-Jeon;Bae, Man-Jong
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.29 no.1
    • /
    • pp.116-119
    • /
    • 2000
  • This study was conducted to investigate antiallergy of the polyphenol fractions isolated from persimmon leaves (Diospyros kaki folum). The result of the passive cutaneous anaphylaxis (PCA) reaction of expriment on anti-allergy, the allergy remarkably decreased in polyphenol fraction I, II and III groups in comparison with the control group. After antigen challenge, histamine content of blood of the polyphenol groups was lowered to near the normal group.

  • PDF