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http://dx.doi.org/10.13048/jkm.13022

Galgeun-tang, an Herbal Formula, Ameliorates Atopic Dermatitis Responses in Dust Mite Extract-treated NC/Nga Mice  

Ha, Hyekyung (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Lee, Jun Kyoung (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Lee, Mee-Young (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Lim, Hye-Sun (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Shin, Hyeunkyoo (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Publication Information
The Journal of Korean Medicine / v.34, no.4, 2013 , pp. 1-11 More about this Journal
Abstract
Objectives: Galgeun-tang (GGT, gegen-tang, kakkon-to), an herbal formula, is used to treat the common cold, fevers, headaches, hangovers and neck and upper back stiffness. The drugs currently used to treat atopic dermatitis (AD) are limited by the significant adverse effects associated with their long-term usage. The need to efficiently manage the AD response while reducing side effects has led to the development of alternative remedies. Methods: To assess the effects of GGT on AD, the anti-inflammatory and anti-AD properties of GGT were evaluated in both in vitro and in vivo systems. Results: Nitric oxide (NO) and histamine production on lipopolysaccharide (LPS)-treated RAW264.7 cells and phorbol-12 myristate 13-acetate (PMA)/A23187-treated MC/9 cells, respectively, were inhibited by GGT. GGT reduced thymus and activation-regulated chemokine (TARC/CCL17) release on TNF-${\alpha}$/IFN-${\gamma}$ stimulated HaCaT cells in a dose-dependent manner. GGT reduced both plasma levels of IgE and histamine and the dermatitis score in house dust mite induced atopic dermatitis-like lesions on NC/Nga mice. However, there were no significant histopathological differences observed between the GGT group and the AD-induced group, such as AD-like lesions in the dorsal skin or ear or mast cell infiltration in the dorsal skin. Conclusions: These results indicate that GGT inhibits chemokine production by keratinocytes and the atopic dermatitis response in NC/Nga mice, suggesting that GGT may be useful as a therapeutic remedy for treating AD and allergic inflammation-related diseases.
Keywords
Galgeun-tang; Herbal formula; Atopic Dermatitis; Dermatophagoides farinae; Nc/Nga mouse;
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1 Jiang D, Chen Y, Hou X, Xu J, Mu X, Chen W. Influence of Paeonia lactiflora roots extract on cAMP-phosphodiesterase activity and related anti-inflammatory action. J Ethnopharmacol 2011;137(1):914-20.   DOI   ScienceOn
2 Tung YT, Chua MT, Wang SY, Chang ST. Anti-inflammation activities of essential oil and its constituents from indigenous cinnamon (Cinnamomum osmophloeum) twigs. Bioresour Technol 2008;99(9):3908-13.   DOI   ScienceOn
3 Yang X, Hu W, Zhang Q, Wang Y, Sun L. Puerarin inhibits C-reactive protein expression via suppression of nuclear factor kappaB activation in lipopolysaccharide-induced peripheral blood mononuclear cells of patients with stable angina pectoris. Basic Clin Pharmacol Toxicol 2010;107(2):637-42.   DOI   ScienceOn
4 Sumiyoshi K, Nakao A, Setoguchi Y, Tsuboi R, Okumura K, Ogawa H. TGF-beta/Smad signaling inhibits IFN-gamma and TNF-alpha-induced TARC (CCL17) production in HaCaT cells. J Dermatol Sci 2003;31(1):53-8.   DOI   ScienceOn
5 Romagnani S. Cytokines and chemoattractants in allergic inflammation. Mol Immunol 2002;38(12-13):881-5.   DOI   ScienceOn
6 Hirota T, Saeki H, Tomita K, Tanaka S, Ebe K, Sakashita M, et al. Variants of C-C motif chemokine 22 (CCL22) are associated with susceptibility to atopic dermatitis: case-control studies. PLoS One 2011;6(11):e26987.   DOI
7 Shimada Y, Takehara K, Sato S. Both Th2 and Th1 chemokines (TARC/CCL17, MDC/CCL22, and Mig/CXCL9) are elevated in sera from patients with atopic dermatitis. J Dermatol Sci 2004;34(3):201-8.   DOI   ScienceOn
8 Fukuyama T, Tajima Y, Hayashi K, Ueda H, Kosaka T. Prior or coinstantaneous oral exposure to environmental immunosuppressive agents aggravates mite allergen-induced atopic dermatitis-like immunoreaction in NC/Nga mice. Toxicology 2011;289(2-3):132-40.   DOI   ScienceOn
9 US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research. Guidance for industry on estimating the maximum safe starting dose in initial clinical trials for therapeutics in adult healthy volunteers. 2005. Available at: URL: http://www.fda.gov/downloads/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/ucm078932.pdf.
10 Hanifin JM, Cooper KD, Ho VC, Kang S, Krafchik BR, Margolis DJ, et al. Guidelines of care for atopic dermatitis, developed in accordance with the American Academy of Dermatology (AAD)/American Academy of Dermatology Association "Administrative Regulations for Evidence-Based Clinical Practice Guidelines". J Am Acad Dermatol 2004;50(3):391-404.   DOI   ScienceOn
11 Boguniewicz M, Fiedler VC, Raimer S, Lawrence ID, Leung DY, Hanifin JM. A randomized, vehicle-controlled trial of tacrolimus ointment for treatment of atopic dermatitis in children. Pediatric Tacrolimus Study Group. J Allergy Clin Immunol 1998;102(4 Pt 1):637-44.   DOI   ScienceOn
12 Tomi NS, Luger TA. The treatment of atopic dermatitis with topical immunomodulators. Clin Dermatol 2003;21(3):215-24.   DOI   ScienceOn
13 Kurokawa M, Tsurita M, Brown J, Fukuda Y, Shiraki K. Effect of interleukin-12 level augmented by Kakkon-to, a herbal medicine, on the early stage of influenza infection in mice. Antiviral Res 2002;56(2):183-8.   DOI   ScienceOn
14 Yamamoto T, Fujiwara K, Yoshida M, Kageyama-Yahara N, Kuramoto H, Shibahara N, et al. Therapeutic effect of kakkonto in a mouse model of food allergy with gastrointestinal symptoms. Int Arch Allergy Immunol 2009;148(3):175-85.   DOI   ScienceOn
15 Ishijima Y, Kawamura T, Kimura A, Kohno A, Okada T, Tsuji T, et al. Toll-like receptor 4-dependent adjuvant activity of Kakkon-to extract exists in the high molecular weight polysaccharide fraction. Int J Immunopathol Pharmacol 2011;24(1):43-54.   DOI
16 Firenzuoli F, Gori L. Herbal medicine today: clinical and research issues. Evid Based Complement Alternat Med 2007;4(Suppl 1):37-40.   DOI
17 Cheng CW, Bian ZX, Wu TX. Systematic review of Chinese herbal medicine for functional constipation. World J Gastroenterol. 2009;15(39):4886-95.   DOI
18 Terui T. Analysis of the mechanism for the development of allergic skin inflammation and the application for its treatment: overview of the pathophysiology of atopic dermatitis. J Pharmacol Sci 2009;110(3):232-6.   DOI   ScienceOn
19 Seo CS, Kim JH, Shin HK. Simultaneous Determination of Albiflorin, Cinnamaldehyde, Cinnamic Acid, Daidzin, Glycyrrhizin, Liquiritin, Paeoniflorin and Puerarin in Galgeun-tang by HPLC-PDA. J Korean Orient Med 2010;31(6):8-15.
20 Asl MN, Hosseinzadeh H. Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds. Phytother Res 2008;22(6):709-24.   DOI   ScienceOn
21 Nagai H, Teramachi H, Tuchiya T. Recent advances in the development of anti-allergic drugs. Allergol Int 2006;55(1):35-42.   DOI   ScienceOn
22 Aoyama H, Tabata N, Tanaka M, Uesugi Y, Tagami H. Successful treatment of resistant facial lesions of atopic dermatitis with 0.1% FK506 ointment. Br J Dermatol 1995;133(3):494-6.   DOI   ScienceOn
23 Wu MS, Yen HR, Chang CW, Peng TY, Hsieh CF, Chen CJ, et al. Mechanism of action of the suppression of influenza virus replication by Ko-Ken Tang through inhibition of the phosphatidylinositol 3-kinase/Akt signaling pathway and viral RNP nuclear export. J Ethnopharmacol 2011;134(3):614-23.   DOI   ScienceOn