• Korean Institute of Interior Design Journal
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• v.16 no.1 s.60
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• pp.21-30
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• 2007

Frank O. Gehry is known to be an architect whose work ranges over different realms, especially between fine arts and architecture. He himself mentioned about this interdisciplinary aspect of his own work: he was inspired by, worked together with, and sometimes directly influenced by contemporary artists. Among the artists, the most influential ones are the sculptors, especially Richard Serra of Post-Minimalism and Claes Oldenburg of Pop Art. Based on this historically known fact, although very brief, this study explores how the features of Post-Minimal sculpture were transferred into Gerhy's architecture, and how Gehry has developed them into his own language. In Post-Minimal sculpture, the main concept 'Anti-Form' was realized by emphasis on materiality, process and intuitiveness of work of in. Those features appeared vividly in Gehry's works especially in his second stage of his life, but seemed to have disappeared in the third stage. However, in the fourth stage, Gehry went beyond the influence of Post-Minimalism, and he created very unique formal language dialectically formed between Post-Minimal sculptural language and his architectural language.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.590-600
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• 2003

The matrix metalloproteinases (MMPs) are a family of enzymes responsible for degrading connective tissue. MMPs catalyze the breakdown of collagen from the extracellular matrix, leading to wrinkle formation and accelerated skin aging. Furthermore, ultraviolet irradiation causes increased expression of certain MMPs. In the extracellular matrix turnover, MMPs are interacting with endogenous regulators named tissue inhibitors of metalloproteinases (TIMPs). Using peptide substrate assays, it has been demonstrated that TIMP-MMP complexes interact highly specifically with $K_{i}$ values of 10$^{-9}$ -10$^{-16}$ M. Therefore applications for TIMP as inhibitor of collagen degradation are suggested for cosmetic anti-aging products to prevent wrinkle formation and loss of elasticity. To date four TIMP proteins (TIMP-1, TIMP-2, TIMP-3 and TIMP-4) have been identified which show a high degree in sequence similarity. The production of human TIMP-2, a 194-residue nonglycosylated protein, was performed by fed-batch culture of Escherichia coli. TIMP-2 accumulated in the bacterial cells in an insoluble form as inclusion bodies. The inclusion bodies were solubilized and the protein refolded to yield the native TIMP-2 in the active form. The integrity of the protein was confirmed by mass analysis, Edman sequencing and gel shift experiments with authentic samples. The inhibitory activity of the refolded and purified TIMP-2 was demonstrated with MMP-1 and MMP-2 assays using synthetic fluorogenic peptide substrates.s.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.2532-2537
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• 2005

FIR(Finite Impulse Response) filter is well known to be ideal for the finite time state-space model, but it requires much computation due to its inherent non-recursive structure especially when the measurement interval grows to a large extent. And often a fixed-lag smoother based on the finite time interval is needed to monitor the soundness of the system model and the measurement model, but the computation burden of FIR-type smoother imposes much restriction of its usage for real-time application. Conventional recursive forms of FIR estimator[1]-[4] could not be used for real time applications, since they are numerically unstable in their recursive equations. To cope with this problem, we suggest a stable recursive form FIR estimator(SRFIR) and its usefulness is demonstrated for designing the real-time fixed-lag smoother on the finite time window through an example of detection of rate bias in the anti-aircraft gun fire control system.

• Journal of the Korean Home Economics Association
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• v.43 no.8 s.210
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• pp.161-174
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• 2005

The purpose of this study was to examine the history and significance of t-shirts used in school that have a dress code to examine the meaning of the various messages that appears on t-shirts and also to examine how these t-shirts are used as form of communication. Many people that have strong social or political views or opinions use t-shirts with message as a means of communicating their opinion and views. Due to the low cost and ease in manufacturing these types of t-shirts, it is possible to express various slogans, messages and drawings. Based on our observations, there were various messages on t-shirts such as, 'no war', 'peace', 'no drugs', 'anti-smocking', 'protect the environment' and 'race or sex discrimination' among others. In this study, t-shirts were grouped based on their messages. T-shirts were put in to the following groups anti- war t-shirts, political t-shirts, t-shirts with messages about social issues and t-shirts with messages about fashion. Both international and domestic t-shirts were examined.

• Biomedical Science Letters
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• v.9 no.3
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• pp.111-121
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• 2003

Recombinant single chain Fv (scFv) antibodies offer many advantages over mouse monoclonal antibodies such as faster clearance from blood, improved tumor localization, reduced human anti-mouse antibody (HAMA) response, and the availability to manipulate the scFv through genetic approaches. The recombinant phage display was constructed using lym-l hybridoma cells as a source of genetic starting material. mRNA was isolated from the corresponding antibodies hybridoma cells. VH and VL cDNA were amplified with RT-PCR and linked with ScFv by linker DNA to form ScFv DNA, which then were inserted into phagemid pCANTAB5E. The phage of positive clones selected with tube containing raji lymphoma cell and infected by competent E. coli HB2151 to express soluble scFv. The scFv lym-l was secreted into the cytosol and culture supernatant and shown to be of expected size (approximately 32 kDa) by western blot. An active scFv lym-l could be produced in E. coli with soluble form and high yield from hybridoma cell line, using phage display system. Immunoreactivity indicated that scFv lym1 showed a potential biding affinity against the raji lymphoma cell as its parental antibody (intact lym-l Ab).

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.4
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• pp.427-436
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• 2018

In this study, we investigated anti-inflammatory and anti-bacterial constituents from Daucus carota var. sativa (carrot) areal parts. For the extract and solvent fractions, the anti-inflammatory activities were examined by measuring the nitric oxide (NO) production using LPS-stimulated RAW 264.7 cells. Among them, the ethyl acetate (EtOAc) fraction decreased the NO level in a dose-dependent manner. To elucidate further anti-inflammatory mechanisms, EtOAc fraction was evaluated by estimating their effects on the production of prostaglandin $E_2$ and pro-inflammatory cytokines as well as on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). As a result, the EtOAc fraction was determined to inhibit the production of $PGE_2$, IL-$1{\beta}$, IL-6 and reduce the iNOS, COX-2 protein expression. Upon the anti-bacterial tests using Staphylococcus epidermidis and Propionibacterium acnes, n-hexane (Hex) and EtOAc fractions showed the most potent activities. Three phytochemicals were isolated form the EtOAc fraction; diosmetin (1), diosmin (2), cynaroside (3). The chemical structures of the isolated compounds were elucidated based on the spectroscopic data including $^1H$ and $^{13}C$ NMR spectra, as well as comparison of the data to the literatures. Anti-inflammatory and anti-bacterial effects were studied for the isolates. All of the compounds (1 - 3) decreased the NO production, effectively. Also, compound 3 showed anti-bacterial activity on P. acnes. Based on these results, D. carota var. sativa extract could be potentially applicable as anti-inflammatory and anti-bacterial ingredients in cosmetic formulations.

• Journal of Fashion Business
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• v.3 no.1
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• pp.147-158
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• 1999

The Purpose of this study is to comprehend the form of Post-modernism in 1980~90 Fashion by comparing to the representative form of Modernism. For empirical data, photographs and critics about fashion were collected from Vogue fashion magazine to differentiate from other studies which is mostly analysed from collection. The results are as follows: The Modernism in fashion can be epitomized simple and functional form as a rational costume and Post-modern fashion expresses all sorts of emotion as a react of rational theme of modernism with manners of anti-formal pluralism such as pastiche, parody and Kitsch. The fashion in 1980-90s in Vogue expressed as follow :1) The Postmodernism is well exploited around 1990 and costrained by traditional and classical Mode. 2) The other main character of Postmodernism, romantic eroticism is broadly expressed with material and slim curved body conscious line. 3) The Modernism and Postmodernism coexisted in the end of 1990, in more matured format.

• Structural Engineering and Mechanics
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• v.64 no.1
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• pp.71-79
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• 2017

In this study, interaction of several interface cracks located between a functionally graded material (FGM) layer and an elastic layer under anti-plane deformation based on the distributed dislocation technique (DDT) is analyzed. The variation of the shear modulus of the functionally graded coating is modeled by an exponential and linear function along the thickness of the layer. The complex Fourier transform is applied to governing equation to derive a system of singular integral equations with Cauchy type kernel. These equations are solved by a numerical method to obtain the stress intensity factors (SIFs) at the crack tips. The effects of non-homogeneity parameters for exponentially and linearly form of shear modulus, the thickness of the layers and the length of crack on the SIFs for several interface cracks are investigated. The results reveal that the magnitude of SIFs decrease with increasing of FG parameter and thickness of FGM layer. The values of SIFs for FGM layer with exponential form is less than the linear form.

• Journal of Fashion Business
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• v.20 no.5
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• pp.87-101
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• 2016

In this study, a variety of influence in the field of design and analysis about the value of a Grunge Design and the creative design inspirations in the field of hair design to help the development of the quality of research. The research methods use the Internet publications such as local and foreign information, analysis and related research and book form, such as the network search, library goes for consideration by a literature search. The contents of this study used review of the case and by Grunge design, expressive characteristics by color, texture and form of Grunge hair design, from 2014 to 2016 trend collections in the last three years through the analysis of design by date of the case. The result of this study is, Grunge design appearing in the areas of hair design, that are grunge anti fashion like the beauty of the disorder, the disharmony, the incomplete, the kitsch, the poverty. Although Grunge means dirt, filth, rubbish as a slang but it is valuable which was raised from the anti fashion to high fashion and alternative of main stream fashion and the hair design in modern fashion also brought. In this study, we can understand the grunge hair design in modern fashion was started from lower place as alternative and forecast the potentialities, the formativeness of the grunge design and value of the beauty and grunge anti fashion the identity and the spirit appearing steadily a modern fashion influence are reflected in our next fashion and design characteristics.

• The Journal of Pediatrics of Korean Medicine
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• v.22 no.1
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• pp.103-111
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• 2008

Objectives Seunggal-tang is one of the prescriptions of oriental herbal medicine, which has been applied to several allergic diseases such as atopic dermatitis. This study was planned to compare differences of anti-allergic effects based on different form of Seunggal-tang by manufacturing differently. Methods Two types herb medicine products were used; aqueous extract (SG-T, Seunggal-Tang) and powder (SG-S, Seunggal-San) which were made from the same mixed formula of Seunggal-tang. To investigate in vitro anti-allergic activities, rat basophilic leukemia (RBL-2H3) cells were treated with SG-T and SG-S for 1 hour, and then stimulated with the phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187. We examined the release of beta-hexosaminidase, as a marker of degranulation, and the releases of tumor necrosis factor (TNF)-alpha and interleukin (IL)-4, as proinflammatory cytokines. Results SG-T and SG-S didn't have effects on cell viabilities in concentrations under 2㎎/㎖. In additionto that, SG-T more inhibited releasing ${\beta}$-hexosaminidase, TNF-${\alpha}$ and IL-4 than SG-S. Conclusions These results indicate that SG-T is more effective against mast cell-mediated allergic reactions than SG-S.