• Journal of Korean Neurosurgical Society
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• 제53권6호
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• pp.323-330
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• 2013

Objective : To develop a simple, reproducible model of disc degeneration in rabbits through percutaneous annular puncture and to confirm the degree of degeneration over time. Methods : Fifteen New Zealand white rabbits (4 to 5 months old and weighing approximately 3 to 3.5 kg each) underwent annular puncture of the L2-L3, L3-L4, and L4-L5 discs. Rabbits were sacrificed at 4, 8, or 20 weeks after puncture. For a longitudinal study to assess changes in disc height over time, serial X-rays were performed at 0, 2, 4, 8, and 20 weeks for rabbits in the 20-week group. Upon sacrifice, the whole spinal column and discs were extracted and analyzed with magnetic resonance imaging (MRI), real time reverse transcriptase-polymerase chain reaction, and histological staining. Results : The X-rays showed a slow, progressive decrease in disc height over time. Significant disc space narrowing compared to preoperative disc height was observed during the time period (p<0.001). The MRI grade, aggrecan, and matrix metalloprotease-13 mRNA expression and hematoxylin and eosin/safranin O/anti-collagen II staining were consistently indicative of degeneration, supporting the results of the X-ray data. Conclusion : Percutaneous annular puncture resulted in slow, reproducible disc degeneration that was confirmed by radiology, biochemistry, and histology. This in vivo model can be used to study and evaluate the safety and efficacy of biologic treatments for degenerative disc disease.

• Journal of Korean Neurosurgical Society
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• 제55권5호
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• pp.237-243
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• 2014

Objective : Symptomatic disc degeneration develops from inflammatory reactions in the annulus fibrosus (AF). Although inflammatory mediators during annular inflammation have been studied, the roles of matrix metalloproteinases (MMPs) and their inhibitors have not been fully elucidated. In this study, we evaluated the production of MMPs and tissue inhibitors of metalloproteinase (TIMPs) during annular inflammation using an in vitro co-culture system. We also examined the effect of notochordal cells on annular inflammation. Methods : Human AF (hAF) pellet was co-cultured for 48 hours with phorbol myristate acetate-stimulated macrophage-like THP-1 cells. hAF pellet and conditioned media (CM) from co-cultured cells were assayed for MMPs, TIMPs, and insulin-like growth factor (IGF)-1 levels using real-time reverse-transcriptase polymerase chain reaction and enzyem-linked immunosorbent assay. To evaluate whether notochordal cells affected MMPs or TIMPs production on annular inflammation, hAF co-cultured with notochordal cells from adult New Zealand White rabbits, were assayed. Results : MMP-1, -3, -9; and TIMP-1 levels were significantly increased in CM of hAF co-cultured with macrophage-like cells compared with hAF alone, whereas TIMP-2 and IGF-1 levels were significantly decreased (p<0.05). After macrophage exposure, hAF produced significantly more MMP-1 and -3 and less TIMP-1 and -2. Interleukin-$1{\beta}$ stimulation enhanced MMP-1 and -3 levels, and significantly diminished TIMP-2 levels. Co-culturing with rabbit notochordal cells did not significantly influence MMPs and TIMPs production or COL1A2 gene expression. Conclusion : Our results indicate that macrophage-like cells evoke annular degeneration through the regulation of major degradative enzymes and their inhibitors, produced by hAF, suggesting that the selective regulation of these enzymes provides future targets for symptomatic disc degeneration therapy.

• 한국광학회지
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• 제12권4호
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• pp.270-275
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• 2001

본논문에서는 구면수차가 포함된 광학 디스크 시스템에 환형 위상변조 apodizer를 적용하여 bump 형태에 따른 재생신호를 스칼라 회절 이론을 이용하여 조사하였다. 이때 구면수차의 영향을 최소로 하는 최적변수를 결정하기 위해 동(pupil)의 반경 r에 대한 파면수차량에 절대값을 취해서 얻는 최대치와 임의의 값의 비로 W$_{R}$을 정의하였으며 원추형과 준원추형 그리고 원추형 bump로 다양한 형태의 bump를 고려하였다. 환형 위상변조 apodizer는 bump 반경과 형태의 변화에 따른 재생신호가 균일한 진폭과 유사한 경향을 나타냈으며 항상높은 최대재생신호를 가졌다. 구면수차의 증가에 따른 최대재생신호의 변화는 아주 작았으며 실제 광학 디시크 시스템에 사용 가능한 0.6 이상의 재생신호를 갖는 신호 bump 반경의 변화가 거의 없었다. 특히 $W_R$ 이 0.4와 0.6인 경우에는 구면수차에 대한 보정효과가 가장 큼을 알 수 있었다.

• Journal of Mechanical Science and Technology
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• 제17권11호
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• pp.1793-1800
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• 2003

The present study addresses an experimental investigation of the near field flow structures of supersonic, dual, coaxial, free, jet, which is discharged from the coaxial annular nozzle. The secondary stream is made from the annular nozzle of a design Mach number of 1.0 and the primary inner stream from a convergent-divergent nozzle. The objective of the present study is to investigate the interactions between the secondary stream and inner supersonic jets. The resulting flow fields are quantified by pitot impact and static pressure measurements and are visualized by using a shadowgraph optical method. The pressure ratios of the primary jet are varied to obtain over-expanded flows and moderately under-expanded flows at the exit of the coaxial nozzle. The pressure ratio of the secondary annular stream is varied between 1.0 and 4.0. The results show that the secondary annular stream significantly changes the Mach disc diameter and location, and the impact pressure distributions. The effects of the secondary annular stream on the primary supersonic jet flow are strongly dependent on whether the primary jet is under-expanded or over-expanded at the exit of the coaxial nozzle.

• 한국소음진동공학회논문집
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• 제17권7호
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• pp.596-604
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• 2007

이 논문은 후판 환형 디스크의 기하학적인 형상이 래디얼 방향 진동에 의해 방사되는 소음에 미치는 영향을 연구하였다. 디스크의 내경을 고정한 상태에서 두께와 외경을 주어진 범위내에서 변경하면서 이론적인 해를 이용하여 래디얼 모드의 고유진동수와 진동모드의 변화를 검토하였다. 이 결과를 이용하여, 해당 형상을 가진 디스크의 래디얼 방향 고유진동에 의해 발생되는 원격음장, 음향파워 및 방사효율을 계산하였다. 이 결과로부터 고유진동에 의한 음향파워와 방사효율을 최소화할 수 있는 기하학적인 형상을 선택하였다. 마지막으로, 최적화된 디스크의 임의 위치에 단위 하모닉 가진을 가했을 경우에 발생되는 음향파워 및 방사효율 스펙트럼을 구하였으며, 전산해석을 통해 그 정확성을 검증하였다. 이 논문에 소개된 방법을 적용하면 주어진 제한 조건을 만족하면서 목표 주파수 범위내에서 래디얼 진동에 의해 발생되는 소음을 최소화할 수 있는 기하학적인 형상을 간편하고 논리적으로 구할 수 있다.

• Coupled systems mechanics
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• 제8권3호
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• pp.273-287
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• 2019

Elastoplastic analysis of an annular disc, being fully constrained on its outer rim and interacting with a purely elastic inclusion perfectly bonded with its inner rim, is conducted to study its plastic deformation and residual stress under thermal cycles. The system is termed the composite disc. Quasi-static plane-strain deformation is assumed, and the von Mises yield criterion with or without the Ludwik hardening rule is adopted in our finite element calculations. Effects of multiple material properties simultaneously being temperature dependent on the plastic behavior of the composite disc are considered. Residual stress is analyzed from a complete loading and unloading cycle. Results are discussed for various inclusion radii. It is found that when temperature dependent material properties are considered, the maximum residual stress may be greater than the maximum stress inside the disc at the temperature-loaded state due to lower temperature having larger yield stress. Temperature independent material properties overestimate stresses inside materials, as well as the elastic irreversible temperature and plastic collapse temperature.

• Journal of Korean Neurosurgical Society
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• 제44권5호
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• pp.327-333
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• 2008

Objective: The purpose of this study is to verify the usefulness of the rabbit model for disc degeneration study. Materials: The L1-L2, L2-L3, L3-L4. or L4-L5 lumbar intervertebral disc (IVD) of 9 mature male New Zealand White rabbits were injured by inserting a 16-gauge needle to a depth of 5 mm in the left anterolateral annulus fibrosus while leaving L5-L6 IVD uninjured. Three other rabbits also received intradiscal injections of rabbit disc cells transfected with adenovirus and bone morphogenetic protein-2 (ad-BMP-2) at L4-L5 in addition to injury by 16-gauge needle at the L1-L2 level. Using digitized radiographs, measurements of IVD height were made and analyzed by using the disc height index (DHI). Magnetic resonance imaging (MRI) scans of the injured discs, injected discs, and uninjured L5-L6 discs were performed at 15 weeks post surgery and compared with preoperative MRI scans. Results: All twelve rabbits showed consistent results of disc degeneration within 15 weeks following annular puncture. DHIs of injured discs were significantly lower than that of the uninjured L5-L6 discs (p<0.05). The mean value of disc degeneration grade of injured discs was significantly higher than that of uninjured discs (p<0.05). The injection of disc cell transfected with ad-BMP-2 did not induce disc regeneration at 15 weeks after injection. Conclusion: This study showed that the injured disc had a significant change in DHI on simple lateral radiograph and disc degeneration grade on MRI scans within 15 weeks in all rabbits. Rabbit annular puncture model can be useful as a disc degeneration model in vivo.

• Journal of information and communication convergence engineering
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• 제16권4호
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• pp.252-257
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• 2018

A wideband double radiator circular disc annular monopole antenna is proposed is this work. The radiators are etched on the surfaces of two Taconic TLY-5 substrates with a circular hole cut out of each of the radiators initially at the centers of the radiators with subsequent downward displacement of the holes. The antenna is designed with a two-step feeding transformer system for impedance matching between the input power source supplied by a $50-{\Omega}$ SMA connector and the monopole radiators. The transformer system improves the bandwidth performance at higher frequencies. The proposed antenna achieves a wideband having the capability of working between 0.645 and 18.775 GHz, corresponding to a -10 dB bandwidth of 186.7% with gain ranging from 0.95 to 8.26 dBi. In comparison to other metal disc planar monopole antennas, the proposed antenna has a small total size width due to the size of the ground plane, which has a diameter 100 mm. The frequency range of the antenna provides applications in global positioning systems, mobile communications, ultra-wideband short distance communications, and wireless computer networks.

• Journal of Korean Neurosurgical Society
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• 제51권3호
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• pp.182-186
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• 2012

In conventional percutaneous disc surgery, introducing instruments into disc space starts by inserting a guide needle into the triangular working zone. However, landing the guide needle tip on the annular window is a challenging step in endoscopic discectomy. Surgeons tend to repeat the needling procedure to reach an optimal position on the annular target. Obturator guiding technique is a modification of standard endoscopic lumbar discectomy, in which, obturator is used to access triangular working zone instead of a guide needle. Obturator guiding technique provides more vivid feedback and easy manipulation. This technique decreases the steps of inserting instruments and takes safer route from the peritoneum.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2005년도 추계학술대회논문집
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• pp.911-914
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• 2005

The vibrational characteristics of the piezoelectric disc for a torsional vibration transducer is theoretically studied in this paper. The characteristic equation of the piezoelectric annular disc has been derived from Newton's End law and Gibb's free energy equations. With an anisotropic material property of the disc, the characteristic equation has yielded resonance frequencies. Numerically-calculated results were compared with the values obtained by finite element analysis and experiments