• Journal of Microbiology and Biotechnology
• /
• 제26권1호
• /
• pp.110-119
• /
• 2016

High-solid anaerobic digestion of sewage sludge achieves highly efficient volatile solid reduction, and production of volatile fatty acid (VFA) and methane compared with conventional low-solid anaerobic digestion. In this study, the potential mechanisms of the better performance in high-solid anaerobic digestion of sewage sludge were investigated by using 454 high-throughput pyrosequencing and real-time PCR to analyze the microbial characteristics in sewage sludge fermentation reactors. The results obtained by 454 highthroughput pyrosequencing revealed that the phyla Chloroflexi, Bacteroidetes, and Firmicutes were the dominant functional microorganisms in high-solid and low-solid anaerobic systems. Meanwhile, the real-time PCR assays showed that high-solid anaerobic digestion significantly increased the number of total bacteria, which enhanced the hydrolysis and acidification of sewage sludge. Further study indicated that the number of total archaea (dominated by Methanosarcina) in a high-solid anaerobic fermentation reactor was also higher than that in a low-solid reactor, resulting in higher VFA consumption and methane production. Hence, the increased key bacteria and methanogenic archaea involved in sewage sludge hydrolysis, acidification, and methanogenesis resulted in the better performance of high-solid anaerobic sewage sludge fermentation.

• 한국물환경학회지
• /
• 제21권5호
• /
• pp.490-495
• /
• 2005

Since there are very limited numbers of thermophilic anaerobic digesters being operated, it is often difficult to start up a new one using sludge from an existing reactor as a seed. However, for obvious reasons it seems few attempts have been made to compare the start-up performance of thermophilic anaerobic digestion using different sources of seed sludges. The purpose of this study was to evaluate the start-up performance of anaerobic digestion using aerobic Waste Activated Sludge (WAS) from a plant and mesophilic Anaerobic Digested Sludge (ADS) as the seed source at both mesophilic ($35^{\circ}C$) and thermophilic ($55^{\circ}C$) temperatures. In this study, two experiments were conducted. First, thermophilic anaerobic reactors were seeded with WAS (VSS = 4,400 mg/L) and ADS (VSS = 14,500 mg/L) to investigate start-up performance with a feed of acetate as well as propionate. The results show that WAS started to produce $CH_4$ soon after acetate feeding without a lag time, while ADS had a lag time of 10 days. When the feed was changed to propionate, WAS removed propionate down to below the detection limit of 10 mg/L, while ADS removed little propionate and produced little $CH_4$. Second, in order to further compare the methanogenic activity of WAS and ADS, both mesophilic and thermophilic reactors were operated. WAS acclimated to anaerobic conditions shortly and after acclimating it produced more $CH_4$ than ADS. WAS at mesophilic temperature biodegraded acetate at the same rate as for thermophilic. However WAS at mesophilic temperature biodegraded propionate at a much faster rate than at thermophilic. WAS as the seed source of anaerobic digestion resulted in much better performance than ADS at both mesophilic and thermophilic temperatures for both acetate and propionate metabolism.

• 한국물환경학회지
• /
• 제26권5호
• /
• pp.810-815
• /
• 2010

Ultrasonic sludge pre-treatment has been studied to enhance the performance of anaerobic digestion by increasing sludge hydrolysis which is regarded as the rate-limiting-step of anaerobic digestion. In this study, the effect of ultrasonic pre-treatment on sludge hydrolysis (solubilization) and methane production was investigated. Sludge solubilization efficiency increased with ultrasonic energy input. However, it is uneconomical to apply more than 720 kJ/L as the solubilization efficiency per energy input declines afterwards. Volatile fatty acids concentration increased after the ultrasonic sludge hydrolysis. Anaerobic batch digestion showed that methane volume reached 64.7 and 84.5 mL after 18 days of incubation with the control sludge and ultrasonically hydrolyzed sludge, respectively. Methane production potential, maximum methane production rate, and the lag time of modified Gompertz equation were changed from 70 mL, 6.4 mL/day, and 1.2 days to 89 mL, 9.6 mL/day, and 0.5 day, respectively, after the ultrasonic sludge treatment. The results proved that ultrasonic pre-treatment contributed significantly not only for the methane production but also for the reduction of anaerobic digestion time which is critical for the performance of anaerobic sludge digestion.

• 디지털정책학회지
• /
• 1권1호
• /
• pp.7-13
• /
• 2022

본 연구는 횡격막에 적용된 테이핑 처치가 30초간의 무산소 운동수행력에 미치는 영향을 알아보고자 실시하였다. 건강한 대학생 18명을 대상으로 실험군(KT)과 대조군(Sham)에 각각 9명씩 무작위 할당되었다. 두 그룹 모두 윙게이트 검사 기반의 사전 측정이 이루어지고 1주일 휴식 후 처치와 함께 사후 측정이 실시되었다. 두 집단간 변인들의 통계는 사전, 사후값의 변화율을 Mann-Whitney U검정으로 실시하였고, 그 결과 대조군의 평균 파워(AP)에서만 통계적으로 유의한 차이가 나타났다. 이는 횡격막 테이핑이 30초 동안의 무산소 운동 수행에 영향을 미치지 않았음을 의미한다. 하지만 30초보다 긴 장기간 무산소 운동에서 유산소 대사가 증가함을 고려할 때 이후 다양한 시간 범위 내 무산소 운동 수행에 대한 추가 연구가 필요할 것으로 생각된다.

• Journal of Biosystems Engineering
• /
• 제43권1호
• /
• pp.37-44
• /
• 2018

Purpose: The objective of this study was to investigate the high-efficiency anaerobic digestion of organic wastes with high fat content. Specifically, the analysis focused on biogas production performance with a focus on carbon number and the double bond count of the long-chain fatty acids (LCFAs), which are hydrolysis products of triglycerides. Methods: Experiments were performed under mesophilic anaerobic conditions with a feed-to-microorganism ratio (F/M) of 1.0. Biogas production performance was analyzed through biogas production patterns, lag-phase, and the time required for 90% biogas production (T90). Results: Biogas production increased when the content of unsaturated LCFAs (containing relatively large numbers of carbon atoms) increased. In substrate containing LCFAs with four or more double bonds, although the initial lag-phase in biogas production was shortened, development of a three-step lag-phase resulted in decreased biogas production. These results suggest that high rates of anaerobic digestion are possible when the LCFAs have high unsaturated fatty acid content with three or fewer double bonds. Conclusions: When various types of LCFAs are digested anaerobically, biogas production performance can be improved if the unsaturated fatty acid content and number of double bonds are optimized for maximum production.

• 한국토양비료학회지
• /
• 제48권2호
• /
• pp.94-99
• /
• 2015

The performance of chamber and bag digesters for solid state anaerobic digestion (SS-AD) of separated solid fraction of swine manure was investigated using lab-scale digester (4,460 mL total volume and 1,800 mL of effective volume) operating at $37^{\circ}C$ for 63 days. The performance of two different digester types was evaluated in terms of the kinetic constants of methane production obtained from the Gompertz and Gaussian equations. Methane production potential of chamber and bag digester was 202 and $218N{\cdot}mL$ $CH_4/g$ VS. Time to produce 95% methane production potential (T95) and calculated effective anaerobic digestion time were 55.5 days and 41.8 days for chamber digester and 52.8 days and 43.5 days for bag digester, respectively. Our results reveal that the performance was not significantly different between chamber and bag digester.

• 한국환경보건학회지
• /
• 제22권3호
• /
• pp.72-80
• /
• 1996

This study was carried to investigate the biodegradability of phenol wastewater in the sluge blanket-packed bed reactor(SBPBR). The reactor consisted of two regions. The lower region was a sludge blanket of 0.5 m height and the upper region was a packed-bed. The phenol and COD concentration of the effluent, the gas production and the composition of gas were measured to determine the performance of the anaerobic wastewater treatment system as the phenol concentration of the influent was increased from 600 to 1800 mg/l. Stable biodegradation of phenol wastewater could be achieved with the anaerobic treatment system from 600 to 1200 mg/l of the influent phenol concentration. But the SBPBR system was getting more serious at 1800 mg/l of influent phenol concentration. At the steady state of the influent phenol concentration of 600-1200 mg/l, the treatment performance showed the phenol removal efficiency of 94.5~96.3%, the COD removal efficiency of 93.3~96% and the gas production of 4.94~9.64 l/day.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
• /
• 제2권2호
• /
• pp.99-108
• /
• 1998

Results obtained from this research showed that the anaerobic contact process was applicable to pear waste with COD removal efficiencies of up to $95\%$ depending on conditions, provided ammonium and phosphate salts were added as well as other nutrients, present in the commercial fertilizer, Milorganite or in yeast extract. These latter materials were required in minimum concentrations of 5 and 1.5 g/L, respectively, in the feed independent of HRT and volatile solids loading rate, with part of the effect due to the mineral fraction. Digestion was satisfactory over the whole range of volatile solids loading rates and liquid retention time of 30 to 0.5 days tested, although treatment efficiency dropped off noticeably between 1 and 0.5 day liquid retention time because of poorer flocculation and separation of anaerobic bacteria. Settling of anaerobic bacteria including methane producing bacteria was related to settling of mixed liquor suspended solids only at 1 to 5 days liquid retention times, at other liquid retention times anaerobic microorganism settled markedly less efficiently than mixed liquor suspended solids. Further studies are being made to provide information of practical and basic interest. Data on the composition of the active fraction of yeast extract might solve many practical nutrient problems encountered with the anaerobic contact process and improve its economics. Further improvement in the flocculation and settling of anaerobic bacteria as well as other bacteria would improve overall performance and allow the use of shorter liquid retention times with dilute waste. Knowledge about the numbers of methane formers present would allow a degree of understanding and control of the process not presently attainable.

• Journal of Microbiology and Biotechnology
• /
• 제22권8호
• /
• pp.1148-1154
• /
• 2012

A novel two-phase anaerobic treatment technology was developed to treat high-concentration organic cassava bioethanol wastewater. The start-up process and contribution of organics (COD, total nitrogen, and $NH_4^+$-N) removal in spatial succession of the whole process and spatial microbial diversity changing when sampling were analyzed. The results of the start-up phase showed that the organic loading rate could reach up to $10kg\;COD/m^3d$, with the COD removal rate remaining over 90% after 25 days. The sample results indicated that the contribution of COD removal in the pre-anaerobic and anaerobic phases was 40% and 60%, respectively, with the highest efficiency of 98.5%; TN and $NH_4^+$-N had decreased to 0.05 g/l and 0.90 g/l, respectively, and the mineralization rate of total nitrogen was 94.8%, 76.56% of which was attributed to the anaerobic part. The microbial diversity changed remarkably among different sample points depending on the physiological characteristics of identified strains. Moraxellaceae, Planococcaceae, and Prevotellaceae were dominant in the pre-anaerobic phase and Bacteroidetes, Campylobacterales, Acinetobacter, Lactobacillus, Clostridium, and Bacillus for the anaerobic phase. Methanosarcinaceae and Methanosaeta were the two main phylotypes in the anaerobic reactor.

• Journal of Microbiology and Biotechnology
• /
• 제22권10호
• /
• pp.1452-1456
• /
• 2012

The anaerobic oxidation of methane (AOM) in anaerobic sewage sludge was characterized. The net methane oxidation was observed in samples amended with methane plus sulfate or with methane alone, whereas methane formation was observed in the samples without methane, indicating that methane oxidation and formation occurred simultaneously. The ratio of the net methane oxidation rate to $H_2S$ formation was 100:1, suggesting that the AOM was not closely associated with sulfate reduction in the anaerobic sludge. The net AOM was positively associated with the methane concentration and sludge dilution ratio. However, the rate of AOM was negatively correlated with organic substrate (acetate) concentration. Therefore, the production and oxidation of methane could be controlled by environmental conditions and dissolved organic compounds in the bulk solution.